t denticola atcc 35405 protease prtp  (ATCC)


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    Name:
    Treponema denticola
    Description:

    Catalog Number:
    35405
    Price:
    None
    Applications:
    Produces major surface proteinProduces methyl-accepting chemotaxis proteinProduces prolyl aminopeptidase proline iminopeptidase
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    Structured Review

    ATCC t denticola atcc 35405 protease prtp
    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. <t>denticola</t> . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola <t>ATCC</t> 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    https://www.bioz.com/result/t denticola atcc 35405 protease prtp/product/ATCC
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    t denticola atcc 35405 protease prtp - by Bioz Stars, 2020-04
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    Images

    1) Product Images from "Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola"

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0071281

    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.
    Figure Legend Snippet: Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Techniques Used:

    Related Articles

    Clone Assay:

    Article Title: Characterization of a novel family of fibronectin-binding proteins with M23 peptidase domains from Treponema denticola
    Article Snippet: Ten genes encoding potential outer membrane proteins were cloned and expressed and the recombinant proteins were purified ( ) and characterized for their abilities to bind plasma or matrix forms of fibronectin ( ). .. Comparisons between the T. pallidum Nichols and T. denticola ATCC 35405 genomes have become possible because they have both been fully sequenced and annotated.

    Centrifugation:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: Protein expression was induced by the addition of isopropyl-β- d -thiogalactopyranoside (IPTG) to 0.2 mM, and the culture was further incubated for 3 h. Cells were collected by centrifugation for 10 min at 4,000 rpm and lysed by suspension in 1× Laemmli sample buffer and repeated passage through a 26-gauge needle. .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ).

    Amplification:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, DNA amplification and detection were performed in a qPCR system (StepOnePlus, Applied Biosystems Life Technologies, Basel, Switzerland), using master mix SYBR Green Master Mix (Applied Biosystems Life Technologies, Basel, Switzerland) for the amplification reaction. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Blocking Assay:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: .. Receptor blocking experiments using rMsp from T. denticola ATCC 35405 confirmed that the N-terminal region of fibronectin is the only region targeted in vitro by T. denticola ATCC 35405. ..

    SYBR Green Assay:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, DNA amplification and detection were performed in a qPCR system (StepOnePlus, Applied Biosystems Life Technologies, Basel, Switzerland), using master mix SYBR Green Master Mix (Applied Biosystems Life Technologies, Basel, Switzerland) for the amplification reaction. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Incubation:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: Protein expression was induced by the addition of isopropyl-β- d -thiogalactopyranoside (IPTG) to 0.2 mM, and the culture was further incubated for 3 h. Cells were collected by centrifugation for 10 min at 4,000 rpm and lysed by suspension in 1× Laemmli sample buffer and repeated passage through a 26-gauge needle. .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ).

    Activity Assay:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: Strains of Treponema species that are deficient in CTLP activity generally show lower levels of binding to fibronectin , even though they appear to express Msp-like proteins. .. The Msp proteins from T. denticola ATCC 35405 and ATCC 35520 show high sequence identity (except for the central V region; Fig. ), whereas the Msp polypeptide from T. denticola OTK is significantly different and antigenically distinct ( ).

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: Several studies have reported that Msp has porin-like channel-forming activity ( , , , ). .. We noted that the immunogenic domain in ATCC 35405 Msp contains a single diproline motif (residues 249 and 250) that is absent in ATCC 33520 Msp. Because the significant inherent flexibility of the proline ring (particularly when doubled) could potentially influence protein structure predictions , we performed an in silico analysis of an ATCC 35405 Msp sequence in which the unique diproline motif was replaced with the cognate glycine-alanine from ATCC 33520 Msp ( ).

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola
    Article Snippet: There were homologues in T. pedis T A4 to a T. denticola ATCC 35405 surface antigen (TDE2258) involved in co-aggregration with the oral species T. forsythia and motility genes indirectly involved in biofilm formation with other bacteria (filament protein, flagellar hook protein) were highly conserved. .. These include the dentilisin operon in T. denticola , which is believed to contribute to periodontal disease by interfering with host signaling pathways and degrade host-cell matrix proteins by its proteolytic activity , .

    In Silico:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: First, we identified a surface-exposed epitope of T. denticola ATCC 35405 Msp within a 23-residue domain in the central region of the protein that lies between the predicted Msp N-terminal (pfam02707, residues 109 to 208) and C-terminal (pfam02722, residues 360 to 543) domains. .. Then, using in silico protein structural modeling, we showed that (i) the proposed structural model in which the N-terminal half of Msp is localized to the periplasmic space is inconsistent with the immunological data and (ii) a proposed model of ATCC 35405 Msp as a β-barrel porin is both similarly predicted for very diverse T. denticola Msp molecules and wholly consistent with the immunological data.), which has been regularly updated to incorporate new sequences and structures and improved analytical techniques ( , ).

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: .. We noted that the immunogenic domain in ATCC 35405 Msp contains a single diproline motif (residues 249 and 250) that is absent in ATCC 33520 Msp. Because the significant inherent flexibility of the proline ring (particularly when doubled) could potentially influence protein structure predictions , we performed an in silico analysis of an ATCC 35405 Msp sequence in which the unique diproline motif was replaced with the cognate glycine-alanine from ATCC 33520 Msp ( ). .. Two of the 5 models of ATCC 35405 with the Gly-Ala substitution for Pro-Pro predicted a single β-barrel structure consistent with identification of Msp as an outer membrane porin (data not shown).

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: .. Then, using in silico protein structural modeling, we showed that (i) the proposed structural model in which the N-terminal half of Msp is localized to the periplasmic space is inconsistent with the immunological data and (ii) a proposed model of ATCC 35405 Msp as a β-barrel porin is both similarly predicted for very diverse T. denticola Msp molecules and wholly consistent with the immunological data.), which has been regularly updated to incorporate new sequences and structures and improved analytical techniques ( , ). ..

    Expressing:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: In this study, recombinant Msp and defined fragments were successfully purified from E. coli , with no deleterious effects on the expression host and with minimal degradation of recombinant polypeptides. .. Receptor blocking experiments using rMsp from T. denticola ATCC 35405 confirmed that the N-terminal region of fibronectin is the only region targeted in vitro by T. denticola ATCC 35405.

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: Paragraph title: Expression of recombinant proteins in E. coli . ... Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ).

    Western Blot:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ). .. SDS-PAGE and Western immunoblotting were performed as described previously ( ).

    Derivative Assay:

    Article Title: Characterization of a novel family of fibronectin-binding proteins with M23 peptidase domains from Treponema denticola
    Article Snippet: Comparisons between the T. pallidum Nichols and T. denticola ATCC 35405 genomes have become possible because they have both been fully sequenced and annotated. .. Analysis of open reading frames suggests that T. pallidum and T. denticola are derived ancestrally.

    Generated:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: We noted that the immunogenic domain in ATCC 35405 Msp contains a single diproline motif (residues 249 and 250) that is absent in ATCC 33520 Msp. Because the significant inherent flexibility of the proline ring (particularly when doubled) could potentially influence protein structure predictions , we performed an in silico analysis of an ATCC 35405 Msp sequence in which the unique diproline motif was replaced with the cognate glycine-alanine from ATCC 33520 Msp ( ). .. To provide further information validating the high-scoring structural models generated by I-TASSER that resembled outer membrane porins, we used the TMBpro server, which is specifically designed to predict the tertiary structure and membrane orientation of transmembrane β-barrel (TMB) proteins embedded in the outer membranes of didermal bacteria ( ).

    Sequencing:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: .. The msp gene of T. denticola ATCC 35405 may be divided into three coding regions on the basis of sequence conservation with msp of T. denticola ATCC 33520 (Fig. ). .. These comprise an N-terminal region of 609 bp; a central, sequence variable (V) region of approximately 200 bp; and a C-terminal region of about 800 bp (Fig. ).

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: .. The Msp proteins from T. denticola ATCC 35405 and ATCC 35520 show high sequence identity (except for the central V region; Fig. ), whereas the Msp polypeptide from T. denticola OTK is significantly different and antigenically distinct ( ). .. The predicted sequence of Msp from T. denticola GM-1 is > 95% identical to that of Msp from strain OTK (A. M. Edwards, unpublished data).

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: .. We noted that the immunogenic domain in ATCC 35405 Msp contains a single diproline motif (residues 249 and 250) that is absent in ATCC 33520 Msp. Because the significant inherent flexibility of the proline ring (particularly when doubled) could potentially influence protein structure predictions , we performed an in silico analysis of an ATCC 35405 Msp sequence in which the unique diproline motif was replaced with the cognate glycine-alanine from ATCC 33520 Msp ( ). .. Two of the 5 models of ATCC 35405 with the Gly-Ala substitution for Pro-Pro predicted a single β-barrel structure consistent with identification of Msp as an outer membrane porin (data not shown).

    Recombinant:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ). .. Receptor blocking experiments using rMsp from T. denticola ATCC 35405 confirmed that the N-terminal region of fibronectin is the only region targeted in vitro by T. denticola ATCC 35405.

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: The msp gene of T. denticola ATCC 35405 may be divided into three coding regions on the basis of sequence conservation with msp of T. denticola ATCC 33520 (Fig. ). .. To determine if these regions encoded Msp fragments with substrate binding properties, recombinant polypeptides corresponding to sequences within the N-terminal (rN-Msp, 14 to 202 aa residues), variable (rV-Msp, 203 to 259 aa residues), and C-terminal (rC-Msp, 272 to 543 aa residues) regions were purified.

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ). .. SDS-PAGE and Western immunoblotting were performed as described previously ( ).

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: The pore diameter of native ATCC 35405 Msp was estimated to be 3.4 nm, based on single-channel conductance studies in black lipid bilayer model membranes , and recombinant Msp has been shown to have similar conductance characteristics ( , ). .. We noted that the immunogenic domain in ATCC 35405 Msp contains a single diproline motif (residues 249 and 250) that is absent in ATCC 33520 Msp. Because the significant inherent flexibility of the proline ring (particularly when doubled) could potentially influence protein structure predictions , we performed an in silico analysis of an ATCC 35405 Msp sequence in which the unique diproline motif was replaced with the cognate glycine-alanine from ATCC 33520 Msp ( ).

    Article Title: Characterization of a novel family of fibronectin-binding proteins with M23 peptidase domains from Treponema denticola
    Article Snippet: Ten genes encoding potential outer membrane proteins were cloned and expressed and the recombinant proteins were purified ( ) and characterized for their abilities to bind plasma or matrix forms of fibronectin ( ). .. Comparisons between the T. pallidum Nichols and T. denticola ATCC 35405 genomes have become possible because they have both been fully sequenced and annotated.

    Crocin Bleaching Assay:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, P. gingivalis (ATCC 33277 T; OMZ 925) was grown anaerobically on Columbia Blood Agar (CBA) agar plates for 3–4 days at 37 °C, followed by anaerobic subculturing for 2–3 days at 37 °C in broth. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    In Vivo:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: Thus, it is likely that CTLP ( ) or other components of the Msp complex may modulate fibronectin binding functions in vivo. .. The Msp proteins from T. denticola ATCC 35405 and ATCC 35520 show high sequence identity (except for the central V region; Fig. ), whereas the Msp polypeptide from T. denticola OTK is significantly different and antigenically distinct ( ).

    Mutagenesis:

    Article Title: Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola
    Article Snippet: .. Wild-type T. denticola ATCC 35405 was found to penetrate the tissue layers effectively, whereas a nonmotile mutant was unable to overcome the tissue barrier. .. Interestingly, the chemotaxis mutants also showed impaired tissue penetration.

    Isolation:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: The Msp proteins from T. denticola ATCC 35405 and ATCC 35520 show high sequence identity (except for the central V region; Fig. ), whereas the Msp polypeptide from T. denticola OTK is significantly different and antigenically distinct ( ). .. In contrast, T. vincentii and some related Treponema species isolated from animal infections show lower levels of binding to fibronectin than T. denticola ( ).

    Labeling:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: We noted that the immunogenic domain in ATCC 35405 Msp contains a single diproline motif (residues 249 and 250) that is absent in ATCC 33520 Msp. Because the significant inherent flexibility of the proline ring (particularly when doubled) could potentially influence protein structure predictions , we performed an in silico analysis of an ATCC 35405 Msp sequence in which the unique diproline motif was replaced with the cognate glycine-alanine from ATCC 33520 Msp ( ). .. The I-TASSER model with the highest score (C score of −1.64) is shown in Fig. S2, with the surface epitope (residues 229 to 251) labeled as in .

    Purification:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: In this study, recombinant Msp and defined fragments were successfully purified from E. coli , with no deleterious effects on the expression host and with minimal degradation of recombinant polypeptides. .. Receptor blocking experiments using rMsp from T. denticola ATCC 35405 confirmed that the N-terminal region of fibronectin is the only region targeted in vitro by T. denticola ATCC 35405.

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: The msp gene of T. denticola ATCC 35405 may be divided into three coding regions on the basis of sequence conservation with msp of T. denticola ATCC 33520 (Fig. ). .. To determine if these regions encoded Msp fragments with substrate binding properties, recombinant polypeptides corresponding to sequences within the N-terminal (rN-Msp, 14 to 202 aa residues), variable (rV-Msp, 203 to 259 aa residues), and C-terminal (rC-Msp, 272 to 543 aa residues) regions were purified.

    Article Title: Characterization of a novel family of fibronectin-binding proteins with M23 peptidase domains from Treponema denticola
    Article Snippet: Ten genes encoding potential outer membrane proteins were cloned and expressed and the recombinant proteins were purified ( ) and characterized for their abilities to bind plasma or matrix forms of fibronectin ( ). .. Comparisons between the T. pallidum Nichols and T. denticola ATCC 35405 genomes have become possible because they have both been fully sequenced and annotated.

    Construct:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: Plasmid DNAs carrying various msp constructs were introduced into the E. coli expression strain C41/pLysS. .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ).

    SDS Page:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: The msp gene of T. denticola ATCC 35405 may be divided into three coding regions on the basis of sequence conservation with msp of T. denticola ATCC 33520 (Fig. ). .. We were unable to express a stable recombinant polypeptide comprising the entire V region (203 to 271 aa residues). rMsp and rC-Msp migrated on SDS-PAGE according to their predicted molecular masses of 53 kDa and 31.7 kDa, respectively (Fig. , lanes 1 and 4).

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ). .. SDS-PAGE and Western immunoblotting were performed as described previously ( ).

    Plasmid Preparation:

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)
    Article Snippet: Plasmid DNAs carrying various msp constructs were introduced into the E. coli expression strain C41/pLysS. .. Samples were subjected to 10% SDS-PAGE and analyzed by Western blotting with rabbit antibodies raised against the ATCC 35405 Msp N-terminal domain (residues 14 to 202) , native ATCC 35405 Msp (this study), recombinant Msp , T. denticola ATCC 35405 cells , or T. denticola FlaA ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Paragraph title: Bacterial quantification by qPCR ... T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Binding Assay:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ). .. Receptor blocking experiments using rMsp from T. denticola ATCC 35405 confirmed that the N-terminal region of fibronectin is the only region targeted in vitro by T. denticola ATCC 35405.

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: Paragraph title: Binding regions of Msp to fibronectin and other host proteins. ... The msp gene of T. denticola ATCC 35405 may be divided into three coding regions on the basis of sequence conservation with msp of T. denticola ATCC 33520 (Fig. ).

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: Strains of T. socranskii and T. pectinovorum have been shown to produce 43-kDa Msp-like proteins , but their binding properties and their relationship to other Msp proteins from T. denticola are unknown. .. The Msp proteins from T. denticola ATCC 35405 and ATCC 35520 show high sequence identity (except for the central V region; Fig. ), whereas the Msp polypeptide from T. denticola OTK is significantly different and antigenically distinct ( ).

    In Vitro:

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405
    Article Snippet: .. Receptor blocking experiments using rMsp from T. denticola ATCC 35405 confirmed that the N-terminal region of fibronectin is the only region targeted in vitro by T. denticola ATCC 35405. ..

    Subculturing Assay:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, P. gingivalis (ATCC 33277 T; OMZ 925) was grown anaerobically on Columbia Blood Agar (CBA) agar plates for 3–4 days at 37 °C, followed by anaerobic subculturing for 2–3 days at 37 °C in broth. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Chemotaxis Assay:

    Article Title: Virulence Factors of the Oral Spirochete Treponema denticola
    Article Snippet: Chemotaxis of oral treponemes toward sera and albumin of rabbit . .. Genetic analysis of Treponema denticola ATCC 35405 biofilm formation .

    Article Title: Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola
    Article Snippet: In this study, we addressed the role of motility and chemotaxis in tissue penetration by the periodontal disease-associated oral spirochete Treponema denticola using an oral epithelial cell line-based experimental approach. .. Wild-type T. denticola ATCC 35405 was found to penetrate the tissue layers effectively, whereas a nonmotile mutant was unable to overcome the tissue barrier.

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    ATCC t denticola atcc 35405 protease prtp
    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. <t>denticola</t> . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola <t>ATCC</t> 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.
    T Denticola Atcc 35405 Protease Prtp, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Article Snippet: Amino acid alignments of T. denticola ATCC 35405 protease PrtP (TDE0762) and identified homologues.

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