t denticola atcc 35404  (ATCC)


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    Treponema denticola C CIP 103920
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    Catalog Number:
    35404
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    Structured Review

    ATCC t denticola atcc 35404
    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. <t>denticola</t> ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    https://www.bioz.com/result/t denticola atcc 35404/product/ATCC
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 35404 - by Bioz Stars, 2020-05
    94/100 stars

    Images

    1) Product Images from "Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment"

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment

    Journal: Infection and Immunity

    doi:

    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.
    Figure Legend Snippet: Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    Techniques Used: Southern Blot, Western Blot

    2) Product Images from "Treponema denticola Is Resistant to Human ?-Defensins "

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.7.3982-3984.2002

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Figure Legend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Techniques Used: Incubation

    3) Product Images from "Treponema denticola Is Resistant to Human ?-Defensins "

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.7.3982-3984.2002

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Figure Legend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Techniques Used: Incubation

    4) Product Images from "Treponema denticola Is Resistant to Human ?-Defensins "

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.7.3982-3984.2002

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Figure Legend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Techniques Used: Incubation

    5) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    6) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    7) Product Images from "Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment"

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment

    Journal: Infection and Immunity

    doi:

    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.
    Figure Legend Snippet: Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    Techniques Used: Southern Blot, Western Blot

    8) Product Images from "Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola"

    Article Title: Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola

    Journal: Infection and Immunity

    doi:

    SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.
    Figure Legend Snippet: SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.

    Techniques Used: SDS Page, Purification, Silver Staining, Autoradiography, Labeling

    9) Product Images from "Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola"

    Article Title: Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola

    Journal: Infection and Immunity

    doi:

    SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.
    Figure Legend Snippet: SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.

    Techniques Used: SDS Page, Purification, Silver Staining, Autoradiography, Labeling

    10) Product Images from "Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model"

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model

    Journal: Infection and Immunity

    doi:

    Virulence of T. denticola ATCC 35404 in mice. (Left) Mice were infected s.c. with 5 × 10 10 T. denticola organisms, and the animals developed a localized lesion (Primary). After recovery from the localized lesion, mice were reinfected with 5 × 10 10 T. denticola organisms (Reinfection). (Center) Lesion formation is also shown for mice actively immunized with T. denticola after (i) primary infection followed by immunization (Primary/Immune), (ii) T. denticola immunization (Immune/Primary), or (iii) primary T. denticola infection followed by control injection with IFA (Primary/Control). The lesion results for the Primary/Immune and Primary/Control groups are following a reinfection with 5 × 10 10 T. denticola organisms. Each bar indicates the mean lesion size from five mice per group in three independent experiments, and the error bars indicate one standard deviation. (Right) Comparison of lesion sizes for P. gingivalis and T. denticola . Mice were infected with 2 × 10 10 P. gingivalis or 5 × 10 10 T. denticola organisms s.c. The bars indicate the mean lesion sizes from groups of 5 to 15 mice. No control results are provided for the lesion data, since mice injected with GM-1 medium and reduced transport fluid developed no lesions. The asterisks indicate results significantly different from those for the immune group ( P
    Figure Legend Snippet: Virulence of T. denticola ATCC 35404 in mice. (Left) Mice were infected s.c. with 5 × 10 10 T. denticola organisms, and the animals developed a localized lesion (Primary). After recovery from the localized lesion, mice were reinfected with 5 × 10 10 T. denticola organisms (Reinfection). (Center) Lesion formation is also shown for mice actively immunized with T. denticola after (i) primary infection followed by immunization (Primary/Immune), (ii) T. denticola immunization (Immune/Primary), or (iii) primary T. denticola infection followed by control injection with IFA (Primary/Control). The lesion results for the Primary/Immune and Primary/Control groups are following a reinfection with 5 × 10 10 T. denticola organisms. Each bar indicates the mean lesion size from five mice per group in three independent experiments, and the error bars indicate one standard deviation. (Right) Comparison of lesion sizes for P. gingivalis and T. denticola . Mice were infected with 2 × 10 10 P. gingivalis or 5 × 10 10 T. denticola organisms s.c. The bars indicate the mean lesion sizes from groups of 5 to 15 mice. No control results are provided for the lesion data, since mice injected with GM-1 medium and reduced transport fluid developed no lesions. The asterisks indicate results significantly different from those for the immune group ( P

    Techniques Used: Mouse Assay, Infection, Injection, Immunofluorescence, Standard Deviation

    11) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    12) Product Images from "Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola"

    Article Title: Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola

    Journal: Infection and Immunity

    doi:

    SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.
    Figure Legend Snippet: SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.

    Techniques Used: SDS Page, Purification, Silver Staining, Autoradiography, Labeling

    Related Articles

    Incubation:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. E. coli incubated in GM-1 medium was used as a control for hβD-2 activity and was quantitated by serial dilution on LB agar.

    Metabolic Labelling:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: .. T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. The proteins were electrophoretically transferred onto nitrocellulose paper (0.25-μm pore size; Schleicher & Schuell, Inc., Keene, N.H.) by using 25 mM Tris–192 mM glycine–20% methanol buffer (pH 8.3) at 8 V/cm for 3 h at 4°C ( ).

    Microscopy:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Purification:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: .. Chu L, Holt S C. Purification and characterization of a 45 kDa hemolysin from Treponema denticola ATCC 35404. ..

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    ATCC t denticola atcc 35405
    Localization of T. <t>denticola</t> major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola <t>ATCC</t> 35405 with or without membrane permeabilization, using polyclonal antibodies raised
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Localization of T. denticola major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola ATCC 35405 with or without membrane permeabilization, using polyclonal antibodies raised

    Journal: Infection and Immunity

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿

    doi: 10.1128/IAI.05701-11

    Figure Lengend Snippet: Localization of T. denticola major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola ATCC 35405 with or without membrane permeabilization, using polyclonal antibodies raised

    Article Snippet: T. denticola ATCC 35405 ( ) and isogenic msp mutant strain MHE ( ) were grown in NOS broth medium as previously described ( , ), with erythromycin (Em) (40 μg ml−1 ) added as appropriate.

    Techniques: Immunofluorescence

    Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Journal: Journal of microbiological methods

    Article Title: A simplified erythromycin resistance cassette for Treponema denticola mutagenesis

    doi: 10.1016/j.mimet.2010.07.020

    Figure Lengend Snippet: Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Article Snippet: T. denticola ATCC 35405 (the Type strain, which has been passaged extensively in various laboratories) was electroporated with the resulting linear DNA s and plated in NOS-GN soft agar medium containing erythromycin (40 μg ml−1 , Sigma Chemical Co., St. Louis, MO) as described previously.

    Techniques:

    Mean areas of lesions at infection sites after challenge with T. denticola ATCC 35405 and prtP mutant K1. Mice were injected with live T. denticola ATCC 35405 (open bars) or mutant K1 (hatched bars), and lesion areas were determined at the indicated times following infection.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Mean areas of lesions at infection sites after challenge with T. denticola ATCC 35405 and prtP mutant K1. Mice were injected with live T. denticola ATCC 35405 (open bars) or mutant K1 (hatched bars), and lesion areas were determined at the indicated times following infection.

    Article Snippet: T. denticola ATCC 35405 was propagated in TYGVS medium , while Porphyromonas gingivalis and Fusobacterium nucleatum were maintained on Tripticase soy agar (Becton Dickinson and Company, Cockeysville, Md.) containing 10% defribrinated horse blood, 5 μg of hemin per ml, and 0.5 μg of menadione per ml.

    Techniques: Infection, Mutagenesis, Mouse Assay, Injection

    Immunoblot analysis of T. denticola ATCC 35405 and K1 with anti- T. denticola ATCC 35405 whole-cell serum. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 and K1 with anti- T. denticola ATCC 35405 whole-cell serum. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Article Snippet: T. denticola ATCC 35405 was propagated in TYGVS medium , while Porphyromonas gingivalis and Fusobacterium nucleatum were maintained on Tripticase soy agar (Becton Dickinson and Company, Cockeysville, Md.) containing 10% defribrinated horse blood, 5 μg of hemin per ml, and 0.5 μg of menadione per ml.

    Techniques:

    Southern blot analysis of T. denticola ATCC 35405 and prtP mutant K1. Chromosomal DNAs from T. denticola ATCC 35405 (lanes 1 and 3) and prtP mutant K1 (lanes 2 and 4) were digested with Hin dIII and hybridized with a digoxigenin-labeled Kpn I- Pst I fragment from the prtP gene (lanes 1 and 2) or the Kpn I- Bam HI fragment from pVA2198 (lanes 3 and 4). Numbers at right are kilobase pairs.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Southern blot analysis of T. denticola ATCC 35405 and prtP mutant K1. Chromosomal DNAs from T. denticola ATCC 35405 (lanes 1 and 3) and prtP mutant K1 (lanes 2 and 4) were digested with Hin dIII and hybridized with a digoxigenin-labeled Kpn I- Pst I fragment from the prtP gene (lanes 1 and 2) or the Kpn I- Bam HI fragment from pVA2198 (lanes 3 and 4). Numbers at right are kilobase pairs.

    Article Snippet: T. denticola ATCC 35405 was propagated in TYGVS medium , while Porphyromonas gingivalis and Fusobacterium nucleatum were maintained on Tripticase soy agar (Becton Dickinson and Company, Cockeysville, Md.) containing 10% defribrinated horse blood, 5 μg of hemin per ml, and 0.5 μg of menadione per ml.

    Techniques: Southern Blot, Mutagenesis, Labeling

    Gelatin zymography of sonicates of T. denticola ATCC 35405 (lane 1) and K1 (lane 2).

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Gelatin zymography of sonicates of T. denticola ATCC 35405 (lane 1) and K1 (lane 2).

    Article Snippet: T. denticola ATCC 35405 was propagated in TYGVS medium , while Porphyromonas gingivalis and Fusobacterium nucleatum were maintained on Tripticase soy agar (Becton Dickinson and Company, Cockeysville, Md.) containing 10% defribrinated horse blood, 5 μg of hemin per ml, and 0.5 μg of menadione per ml.

    Techniques: Zymography

    SDS-PAGE analysis of sonicates of T. denticola ATCC 35405 and K1. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). After electrophoresis, the gel was stained with Coomassie brilliant blue R-250. Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: SDS-PAGE analysis of sonicates of T. denticola ATCC 35405 and K1. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). After electrophoresis, the gel was stained with Coomassie brilliant blue R-250. Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Article Snippet: T. denticola ATCC 35405 was propagated in TYGVS medium , while Porphyromonas gingivalis and Fusobacterium nucleatum were maintained on Tripticase soy agar (Becton Dickinson and Company, Cockeysville, Md.) containing 10% defribrinated horse blood, 5 μg of hemin per ml, and 0.5 μg of menadione per ml.

    Techniques: SDS Page, Electrophoresis, Staining

    Immunoblot analysis of T. denticola ATCC 35405 (lane 1; boiled) and K1 (lane 2; boiled) with antidentilisin serum.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 (lane 1; boiled) and K1 (lane 2; boiled) with antidentilisin serum.

    Article Snippet: T. denticola ATCC 35405 was propagated in TYGVS medium , while Porphyromonas gingivalis and Fusobacterium nucleatum were maintained on Tripticase soy agar (Becton Dickinson and Company, Cockeysville, Md.) containing 10% defribrinated horse blood, 5 μg of hemin per ml, and 0.5 μg of menadione per ml.

    Techniques:

    MSP amino acid sequence alignment of T. denticola strains ATCC 35405 and Group A (panel A), and ATCC 33520 and Group B (panel B) . The grey areas indicate single amino acid substitutions compared with ATCC 35405 (panel A) and ATCC 33520 (panel B).

    Journal: BMC Infectious Diseases

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis

    doi: 10.1186/1471-2334-10-345

    Figure Lengend Snippet: MSP amino acid sequence alignment of T. denticola strains ATCC 35405 and Group A (panel A), and ATCC 33520 and Group B (panel B) . The grey areas indicate single amino acid substitutions compared with ATCC 35405 (panel A) and ATCC 33520 (panel B).

    Article Snippet: Based on these findings, the 17 samples were divided into 2 distinct groups, A (15 samples) and B (the remaining 2), whose msp nucleotide sequences were closely related to T. denticola ATCC 35405 and ATCC 33520, respectively.

    Techniques: Sequencing

    Diversity of msp sequences . Sequence alignments of 17 central regions (from 600 to 900 nucleotides) from T. denticola positive clinical specimens. In panel A are shown the clinical samples of Group A. In panel B are shown the clinical samples of Group B. The upper line contains the sequence of T. denticola strain ATCC 35405 and ATCC 33520, respectively, in both panels. The grey areas indicate variations of single nucleotide positions compared with T. denticola ATCC 35405.

    Journal: BMC Infectious Diseases

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis

    doi: 10.1186/1471-2334-10-345

    Figure Lengend Snippet: Diversity of msp sequences . Sequence alignments of 17 central regions (from 600 to 900 nucleotides) from T. denticola positive clinical specimens. In panel A are shown the clinical samples of Group A. In panel B are shown the clinical samples of Group B. The upper line contains the sequence of T. denticola strain ATCC 35405 and ATCC 33520, respectively, in both panels. The grey areas indicate variations of single nucleotide positions compared with T. denticola ATCC 35405.

    Article Snippet: Based on these findings, the 17 samples were divided into 2 distinct groups, A (15 samples) and B (the remaining 2), whose msp nucleotide sequences were closely related to T. denticola ATCC 35405 and ATCC 33520, respectively.

    Techniques: Sequencing

    Evolutionary relationships of T. denticola msp gene, deduced from the sequences of 17 clinical samples, ATCC 35405, ATCC 33520, and OTK . The phylogenetic analysis was performed using the neighbor-joining method with MEGA 4 on aligned sequences from the msp complete cds sequence (bootstrap values > 75 are shown at nodes).

    Journal: BMC Infectious Diseases

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis

    doi: 10.1186/1471-2334-10-345

    Figure Lengend Snippet: Evolutionary relationships of T. denticola msp gene, deduced from the sequences of 17 clinical samples, ATCC 35405, ATCC 33520, and OTK . The phylogenetic analysis was performed using the neighbor-joining method with MEGA 4 on aligned sequences from the msp complete cds sequence (bootstrap values > 75 are shown at nodes).

    Article Snippet: Based on these findings, the 17 samples were divided into 2 distinct groups, A (15 samples) and B (the remaining 2), whose msp nucleotide sequences were closely related to T. denticola ATCC 35405 and ATCC 33520, respectively.

    Techniques: Sequencing

    Antigenicity plot of T. denticola strains ATCC 35405 (frame A), ATCC 33520 (frame B), and 2 representative specimens (B66, frame C; B23, frame D) . In each frame, the area of the plot that is surrounded by a continuous line represents the central region of MSP. The internal smaller area, surrounded by the dotted line, represents the portion that has the greatest difference in predicted antigenicity between specimens B66 and B23 and ATCC35405.

    Journal: BMC Infectious Diseases

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis

    doi: 10.1186/1471-2334-10-345

    Figure Lengend Snippet: Antigenicity plot of T. denticola strains ATCC 35405 (frame A), ATCC 33520 (frame B), and 2 representative specimens (B66, frame C; B23, frame D) . In each frame, the area of the plot that is surrounded by a continuous line represents the central region of MSP. The internal smaller area, surrounded by the dotted line, represents the portion that has the greatest difference in predicted antigenicity between specimens B66 and B23 and ATCC35405.

    Article Snippet: Based on these findings, the 17 samples were divided into 2 distinct groups, A (15 samples) and B (the remaining 2), whose msp nucleotide sequences were closely related to T. denticola ATCC 35405 and ATCC 33520, respectively.

    Techniques: