t denticola atcc 35404  (ATCC)


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    Name:
    Treponema denticola C CIP 103920
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    Catalog Number:
    35404
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    Structured Review

    ATCC t denticola atcc 35404
    T. <t>denticola</t> proteases are not responsible for resistance to hβD-2. (A) T. denticola <t>ATCC</t> 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    https://www.bioz.com/result/t denticola atcc 35404/product/ATCC
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 35404 - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Treponema denticola Is Resistant to Human ?-Defensins "

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.7.3982-3984.2002

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Figure Legend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Techniques Used: Incubation

    2) Product Images from "Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment"

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment

    Journal: Infection and Immunity

    doi:

    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.
    Figure Legend Snippet: Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    Techniques Used: Southern Blot, Western Blot

    3) Product Images from "Treponema denticola Is Resistant to Human ?-Defensins "

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.7.3982-3984.2002

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Figure Legend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Techniques Used: Incubation

    4) Product Images from "Treponema denticola Is Resistant to Human ?-Defensins "

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.7.3982-3984.2002

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Figure Legend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Techniques Used: Incubation

    5) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    6) Product Images from "Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola"

    Article Title: Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola

    Journal: Infection and Immunity

    doi:

    SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.
    Figure Legend Snippet: SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.

    Techniques Used: SDS Page, Purification, Silver Staining, Autoradiography, Labeling

    7) Product Images from "Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment"

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment

    Journal: Infection and Immunity

    doi:

    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.
    Figure Legend Snippet: Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    Techniques Used: Southern Blot, Western Blot

    8) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    9) Product Images from "Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola"

    Article Title: Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola

    Journal: Infection and Immunity

    doi:

    SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.
    Figure Legend Snippet: SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.

    Techniques Used: SDS Page, Purification, Silver Staining, Autoradiography, Labeling

    10) Product Images from "Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model"

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model

    Journal: Infection and Immunity

    doi:

    Virulence of T. denticola ATCC 35404 in mice. (Left) Mice were infected s.c. with 5 × 10 10 T. denticola organisms, and the animals developed a localized lesion (Primary). After recovery from the localized lesion, mice were reinfected with 5 × 10 10 T. denticola organisms (Reinfection). (Center) Lesion formation is also shown for mice actively immunized with T. denticola after (i) primary infection followed by immunization (Primary/Immune), (ii) T. denticola immunization (Immune/Primary), or (iii) primary T. denticola infection followed by control injection with IFA (Primary/Control). The lesion results for the Primary/Immune and Primary/Control groups are following a reinfection with 5 × 10 10 T. denticola organisms. Each bar indicates the mean lesion size from five mice per group in three independent experiments, and the error bars indicate one standard deviation. (Right) Comparison of lesion sizes for P. gingivalis and T. denticola . Mice were infected with 2 × 10 10 P. gingivalis or 5 × 10 10 T. denticola organisms s.c. The bars indicate the mean lesion sizes from groups of 5 to 15 mice. No control results are provided for the lesion data, since mice injected with GM-1 medium and reduced transport fluid developed no lesions. The asterisks indicate results significantly different from those for the immune group ( P
    Figure Legend Snippet: Virulence of T. denticola ATCC 35404 in mice. (Left) Mice were infected s.c. with 5 × 10 10 T. denticola organisms, and the animals developed a localized lesion (Primary). After recovery from the localized lesion, mice were reinfected with 5 × 10 10 T. denticola organisms (Reinfection). (Center) Lesion formation is also shown for mice actively immunized with T. denticola after (i) primary infection followed by immunization (Primary/Immune), (ii) T. denticola immunization (Immune/Primary), or (iii) primary T. denticola infection followed by control injection with IFA (Primary/Control). The lesion results for the Primary/Immune and Primary/Control groups are following a reinfection with 5 × 10 10 T. denticola organisms. Each bar indicates the mean lesion size from five mice per group in three independent experiments, and the error bars indicate one standard deviation. (Right) Comparison of lesion sizes for P. gingivalis and T. denticola . Mice were infected with 2 × 10 10 P. gingivalis or 5 × 10 10 T. denticola organisms s.c. The bars indicate the mean lesion sizes from groups of 5 to 15 mice. No control results are provided for the lesion data, since mice injected with GM-1 medium and reduced transport fluid developed no lesions. The asterisks indicate results significantly different from those for the immune group ( P

    Techniques Used: Mouse Assay, Infection, Injection, Immunofluorescence, Standard Deviation

    11) Product Images from "Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola"

    Article Title: Activation of Murine Macrophages by Lipoprotein and Lipooligosaccharide of Treponema denticola

    Journal: Infection and Immunity

    doi:

    SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.
    Figure Legend Snippet: SDS-PAGE of the purified LOS of T. denticola ATCC 35404. Lane 1, silver stain; lane 2, autoradiography of the cis [9- 3 H]octadecanoic acid-labeled LOS.

    Techniques Used: SDS Page, Purification, Silver Staining, Autoradiography, Labeling

    12) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    Related Articles

    Incubation:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. E. coli incubated in GM-1 medium was used as a control for hβD-2 activity and was quantitated by serial dilution on LB agar.

    Metabolic Labelling:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: .. T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. The proteins were electrophoretically transferred onto nitrocellulose paper (0.25-μm pore size; Schleicher & Schuell, Inc., Keene, N.H.) by using 25 mM Tris–192 mM glycine–20% methanol buffer (pH 8.3) at 8 V/cm for 3 h at 4°C ( ).

    Microscopy:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Purification:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: .. Chu L, Holt S C. Purification and characterization of a 45 kDa hemolysin from Treponema denticola ATCC 35404. ..

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    ATCC wild type t denticola atcc 35405
    Motility of wild-type T. <t>denticola</t> <t>ATCC</t> 35405 in KBM for epithelial cell lines in a 5% CO 2 atmosphere at 35°C (○) and under anaerobic conditions (85% N 2 , 10% H 2 , 5% CO 2 ) at 35°C (□). Values for each condition were obtained in triplicate in two independent experiments. More than 600 cells were examined for cellular motility in each experiment. All mutant derivatives except the nonmotile strain HL53 showed similar results (data not shown).
    Wild Type T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type t denticola atcc 35405/product/ATCC
    Average 99 stars, based on 25 article reviews
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    Motility of wild-type T. denticola ATCC 35405 in KBM for epithelial cell lines in a 5% CO 2 atmosphere at 35°C (○) and under anaerobic conditions (85% N 2 , 10% H 2 , 5% CO 2 ) at 35°C (□). Values for each condition were obtained in triplicate in two independent experiments. More than 600 cells were examined for cellular motility in each experiment. All mutant derivatives except the nonmotile strain HL53 showed similar results (data not shown).

    Journal: Infection and Immunity

    Article Title: Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola

    doi: 10.1128/IAI.69.10.6276-6283.2001

    Figure Lengend Snippet: Motility of wild-type T. denticola ATCC 35405 in KBM for epithelial cell lines in a 5% CO 2 atmosphere at 35°C (○) and under anaerobic conditions (85% N 2 , 10% H 2 , 5% CO 2 ) at 35°C (□). Values for each condition were obtained in triplicate in two independent experiments. More than 600 cells were examined for cellular motility in each experiment. All mutant derivatives except the nonmotile strain HL53 showed similar results (data not shown).

    Article Snippet: Wild-type T. denticola ATCC 35405 was found to penetrate the tissue layers effectively, whereas a nonmotile mutant was unable to overcome the tissue barrier.

    Techniques: Mutagenesis

    Correlation of penetration rate and tissue resistance. Penetration rates were determined after 8 h of coincubation of the tissues with wild-type T. denticola . Tissue resistance was measured before and after the experiment.

    Journal: Infection and Immunity

    Article Title: Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola

    doi: 10.1128/IAI.69.10.6276-6283.2001

    Figure Lengend Snippet: Correlation of penetration rate and tissue resistance. Penetration rates were determined after 8 h of coincubation of the tissues with wild-type T. denticola . Tissue resistance was measured before and after the experiment.

    Article Snippet: Wild-type T. denticola ATCC 35405 was found to penetrate the tissue layers effectively, whereas a nonmotile mutant was unable to overcome the tissue barrier.

    Techniques:

    Tissue penetration rates (A) and motility (B) of wild-type T. denticola (Td), T. pallidum (Tp), and T. phagedenis (Tph) and various T. denticola motility and chemotaxis mutant strains. Values were determined after 8 h of coincubation of the bacteria with the tissue. Tissues used for these experiments exhibited resistances between 11 and 14Ω. More than 600 cells of each strain were examined for cellular motility.

    Journal: Infection and Immunity

    Article Title: Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola

    doi: 10.1128/IAI.69.10.6276-6283.2001

    Figure Lengend Snippet: Tissue penetration rates (A) and motility (B) of wild-type T. denticola (Td), T. pallidum (Tp), and T. phagedenis (Tph) and various T. denticola motility and chemotaxis mutant strains. Values were determined after 8 h of coincubation of the bacteria with the tissue. Tissues used for these experiments exhibited resistances between 11 and 14Ω. More than 600 cells of each strain were examined for cellular motility.

    Article Snippet: Wild-type T. denticola ATCC 35405 was found to penetrate the tissue layers effectively, whereas a nonmotile mutant was unable to overcome the tissue barrier.

    Techniques: Chemotaxis Assay, Mutagenesis

    Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Journal: Journal of microbiological methods

    Article Title: A simplified erythromycin resistance cassette for Treponema denticola mutagenesis

    doi: 10.1016/j.mimet.2010.07.020

    Figure Lengend Snippet: Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Article Snippet: T. denticola ATCC 35405 (the Type strain, which has been passaged extensively in various laboratories) was electroporated with the resulting linear DNA s and plated in NOS-GN soft agar medium containing erythromycin (40 μg ml−1 , Sigma Chemical Co., St. Louis, MO) as described previously.

    Techniques:

    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Article Snippet: The wide set of T. pedis genes was compared to genes in T. denticola ATCC 35405 complemented with genes from 12 additional T. denticola draft WGS assemblies.

    Techniques: