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t cell immune regulator 1  (Novus Biologicals)


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    Novus Biologicals t cell immune regulator 1
    T Cell Immune Regulator 1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    t cell immune regulator 1 - by Bioz Stars, 2025-04
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    Primers used for quantitative real-time PCR analysis of genes expressions.
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    RAW264.7 macrophages were infected with either L . major Δ gp63 + gp63 or Δ gp63 opsonized metacyclic promastigotes for 6 h. Lysates were placed in a sucrose gradient and fractionated from the top. (A) Western blot showing the presence of GP63 and LPG in light (2–4) or denser fractions (5–8). GRP78, CNX, CRT, and PDI were used as ER markers, Sec22b as an ERGIC marker, and <t>TCIRG1</t> as a maker of endosomes and lysosomes. Light vesicle-containing fractions are delimited by the exclusive appearance of LC3B-II, which is membrane-bound. TCL, total cell lysate. (B) BMM were infected with opsonized L . major Δ gp63 + gp63 metacyclic promastigotes for 6 h and the colocalization (white pixels, middle and rightmost panels) of GP63 (green) or PGs (red) with ER marker Sec23 (blue), or ERGIC markers ERGIC53 (blue) and Sec22b (blue) was assessed by confocal immunofluorescence microscopy. DNA is in cyan. 5X-enlarged insets of representative cytoplasmic regions are shown. White arrowheads denote internalized parasites. Bar, 5 μm. (C) (i) Immuno-electron microscopy image (bar, 500 nm) of a representative 6 h-infected BMM stained for Sec23 (20 nm nanoparticles) and GP63 (10 nm nanoparticles). Red arrowheads denote regions where both Sec23 and GP63 were in close proximity, two of which were magnified (bar, 100 nm) in rightmost panels (ii) and (iii). Lm , L . major -containing PV; N, BMM nucleus. (D) Enzyme protection assay with vesicles that were pelleted from fraction 6 and treated with Prot K ± Triton X-100 or PI-PLC. The protection of GP63 from these enzymes was compared to that of the host’s Sec22b, which faces the cytoplasmic side and is not GPI-anchored. These results are representative of at least two independent experiments.
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    RAW264.7 macrophages were infected with either L . major Δ gp63 + gp63 or Δ gp63 opsonized metacyclic promastigotes for 6 h. Lysates were placed in a sucrose gradient and fractionated from the top. (A) Western blot showing the presence of GP63 and LPG in light (2–4) or denser fractions (5–8). GRP78, CNX, CRT, and PDI were used as ER markers, Sec22b as an ERGIC marker, and <t>TCIRG1</t> as a maker of endosomes and lysosomes. Light vesicle-containing fractions are delimited by the exclusive appearance of LC3B-II, which is membrane-bound. TCL, total cell lysate. (B) BMM were infected with opsonized L . major Δ gp63 + gp63 metacyclic promastigotes for 6 h and the colocalization (white pixels, middle and rightmost panels) of GP63 (green) or PGs (red) with ER marker Sec23 (blue), or ERGIC markers ERGIC53 (blue) and Sec22b (blue) was assessed by confocal immunofluorescence microscopy. DNA is in cyan. 5X-enlarged insets of representative cytoplasmic regions are shown. White arrowheads denote internalized parasites. Bar, 5 μm. (C) (i) Immuno-electron microscopy image (bar, 500 nm) of a representative 6 h-infected BMM stained for Sec23 (20 nm nanoparticles) and GP63 (10 nm nanoparticles). Red arrowheads denote regions where both Sec23 and GP63 were in close proximity, two of which were magnified (bar, 100 nm) in rightmost panels (ii) and (iii). Lm , L . major -containing PV; N, BMM nucleus. (D) Enzyme protection assay with vesicles that were pelleted from fraction 6 and treated with Prot K ± Triton X-100 or PI-PLC. The protection of GP63 from these enzymes was compared to that of the host’s Sec22b, which faces the cytoplasmic side and is not GPI-anchored. These results are representative of at least two independent experiments.
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    RAW264.7 macrophages were infected with either L . major Δ gp63 + gp63 or Δ gp63 opsonized metacyclic promastigotes for 6 h. Lysates were placed in a sucrose gradient and fractionated from the top. (A) Western blot showing the presence of GP63 and LPG in light (2–4) or denser fractions (5–8). GRP78, CNX, CRT, and PDI were used as ER markers, Sec22b as an ERGIC marker, and <t>TCIRG1</t> as a maker of endosomes and lysosomes. Light vesicle-containing fractions are delimited by the exclusive appearance of LC3B-II, which is membrane-bound. TCL, total cell lysate. (B) BMM were infected with opsonized L . major Δ gp63 + gp63 metacyclic promastigotes for 6 h and the colocalization (white pixels, middle and rightmost panels) of GP63 (green) or PGs (red) with ER marker Sec23 (blue), or ERGIC markers ERGIC53 (blue) and Sec22b (blue) was assessed by confocal immunofluorescence microscopy. DNA is in cyan. 5X-enlarged insets of representative cytoplasmic regions are shown. White arrowheads denote internalized parasites. Bar, 5 μm. (C) (i) Immuno-electron microscopy image (bar, 500 nm) of a representative 6 h-infected BMM stained for Sec23 (20 nm nanoparticles) and GP63 (10 nm nanoparticles). Red arrowheads denote regions where both Sec23 and GP63 were in close proximity, two of which were magnified (bar, 100 nm) in rightmost panels (ii) and (iii). Lm , L . major -containing PV; N, BMM nucleus. (D) Enzyme protection assay with vesicles that were pelleted from fraction 6 and treated with Prot K ± Triton X-100 or PI-PLC. The protection of GP63 from these enzymes was compared to that of the host’s Sec22b, which faces the cytoplasmic side and is not GPI-anchored. These results are representative of at least two independent experiments.
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    Primers used for quantitative real-time PCR analysis of genes expressions.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Zinc and Copper with New Triazine Hydrazone Ligand: Two Novel Organic Complexes Enhanced Expression of Peptide Growth Factors and Cytokine Genes in Weaned V-Line Rabbit

    doi: 10.3390/ani9121134

    Figure Lengend Snippet: Primers used for quantitative real-time PCR analysis of genes expressions.

    Article Snippet: Then, qRT-PCR was performed to determine the expression of seven target genes of insulin-like growth factor-1 ( IGF-1 ), growth hormone receptor ( GHR ), fibroblast growth factor 1 ( FGF1 ), transforming growth factor beta-1 ( TGFB1 ), T-cell immune regulator 1 ( TCIRG1 ), interleukin 10 ( IL10 ), and adenosine deaminase ( ADA ), in both liver and spleen tissues, using the ABI 7500 Realtime Detection System (Applied Biosystems, Foster City, CA, USA) and qRT-PCR reagents (TransGen Biotech, Beijing, China).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing, Amplification

    The qRT-PCR validation of mRNA expression for IGF-1 , GHR, FGF1, TGFB1, TCIRG1, IL10 , and ADA in liver and spleen tissue among groups of control, Zn/CuSO 4 , Zn/Cu–Mnt, and Zn/Cu–Thz. IGF-1 : insulin like growth factor 1, GHR : growth hormone receptor, FGF1 : fibroblast growth factor 1, TGFB1 : transforming growth factor beta-1, TCIRG1 : T-cell immune regulator 1, IL10 : interleukin 10, ADA : adenosine deaminase. cDNA samples, liver/group ( n = 5) and spleen/group ( n = 5).

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Zinc and Copper with New Triazine Hydrazone Ligand: Two Novel Organic Complexes Enhanced Expression of Peptide Growth Factors and Cytokine Genes in Weaned V-Line Rabbit

    doi: 10.3390/ani9121134

    Figure Lengend Snippet: The qRT-PCR validation of mRNA expression for IGF-1 , GHR, FGF1, TGFB1, TCIRG1, IL10 , and ADA in liver and spleen tissue among groups of control, Zn/CuSO 4 , Zn/Cu–Mnt, and Zn/Cu–Thz. IGF-1 : insulin like growth factor 1, GHR : growth hormone receptor, FGF1 : fibroblast growth factor 1, TGFB1 : transforming growth factor beta-1, TCIRG1 : T-cell immune regulator 1, IL10 : interleukin 10, ADA : adenosine deaminase. cDNA samples, liver/group ( n = 5) and spleen/group ( n = 5).

    Article Snippet: Then, qRT-PCR was performed to determine the expression of seven target genes of insulin-like growth factor-1 ( IGF-1 ), growth hormone receptor ( GHR ), fibroblast growth factor 1 ( FGF1 ), transforming growth factor beta-1 ( TGFB1 ), T-cell immune regulator 1 ( TCIRG1 ), interleukin 10 ( IL10 ), and adenosine deaminase ( ADA ), in both liver and spleen tissues, using the ABI 7500 Realtime Detection System (Applied Biosystems, Foster City, CA, USA) and qRT-PCR reagents (TransGen Biotech, Beijing, China).

    Techniques: Quantitative RT-PCR, Expressing

    RAW264.7 macrophages were infected with either L . major Δ gp63 + gp63 or Δ gp63 opsonized metacyclic promastigotes for 6 h. Lysates were placed in a sucrose gradient and fractionated from the top. (A) Western blot showing the presence of GP63 and LPG in light (2–4) or denser fractions (5–8). GRP78, CNX, CRT, and PDI were used as ER markers, Sec22b as an ERGIC marker, and TCIRG1 as a maker of endosomes and lysosomes. Light vesicle-containing fractions are delimited by the exclusive appearance of LC3B-II, which is membrane-bound. TCL, total cell lysate. (B) BMM were infected with opsonized L . major Δ gp63 + gp63 metacyclic promastigotes for 6 h and the colocalization (white pixels, middle and rightmost panels) of GP63 (green) or PGs (red) with ER marker Sec23 (blue), or ERGIC markers ERGIC53 (blue) and Sec22b (blue) was assessed by confocal immunofluorescence microscopy. DNA is in cyan. 5X-enlarged insets of representative cytoplasmic regions are shown. White arrowheads denote internalized parasites. Bar, 5 μm. (C) (i) Immuno-electron microscopy image (bar, 500 nm) of a representative 6 h-infected BMM stained for Sec23 (20 nm nanoparticles) and GP63 (10 nm nanoparticles). Red arrowheads denote regions where both Sec23 and GP63 were in close proximity, two of which were magnified (bar, 100 nm) in rightmost panels (ii) and (iii). Lm , L . major -containing PV; N, BMM nucleus. (D) Enzyme protection assay with vesicles that were pelleted from fraction 6 and treated with Prot K ± Triton X-100 or PI-PLC. The protection of GP63 from these enzymes was compared to that of the host’s Sec22b, which faces the cytoplasmic side and is not GPI-anchored. These results are representative of at least two independent experiments.

    Journal: PLoS Pathogens

    Article Title: The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole

    doi: 10.1371/journal.ppat.1007982

    Figure Lengend Snippet: RAW264.7 macrophages were infected with either L . major Δ gp63 + gp63 or Δ gp63 opsonized metacyclic promastigotes for 6 h. Lysates were placed in a sucrose gradient and fractionated from the top. (A) Western blot showing the presence of GP63 and LPG in light (2–4) or denser fractions (5–8). GRP78, CNX, CRT, and PDI were used as ER markers, Sec22b as an ERGIC marker, and TCIRG1 as a maker of endosomes and lysosomes. Light vesicle-containing fractions are delimited by the exclusive appearance of LC3B-II, which is membrane-bound. TCL, total cell lysate. (B) BMM were infected with opsonized L . major Δ gp63 + gp63 metacyclic promastigotes for 6 h and the colocalization (white pixels, middle and rightmost panels) of GP63 (green) or PGs (red) with ER marker Sec23 (blue), or ERGIC markers ERGIC53 (blue) and Sec22b (blue) was assessed by confocal immunofluorescence microscopy. DNA is in cyan. 5X-enlarged insets of representative cytoplasmic regions are shown. White arrowheads denote internalized parasites. Bar, 5 μm. (C) (i) Immuno-electron microscopy image (bar, 500 nm) of a representative 6 h-infected BMM stained for Sec23 (20 nm nanoparticles) and GP63 (10 nm nanoparticles). Red arrowheads denote regions where both Sec23 and GP63 were in close proximity, two of which were magnified (bar, 100 nm) in rightmost panels (ii) and (iii). Lm , L . major -containing PV; N, BMM nucleus. (D) Enzyme protection assay with vesicles that were pelleted from fraction 6 and treated with Prot K ± Triton X-100 or PI-PLC. The protection of GP63 from these enzymes was compared to that of the host’s Sec22b, which faces the cytoplasmic side and is not GPI-anchored. These results are representative of at least two independent experiments.

    Article Snippet: Rabbit polyclonal antibodies anti-Sec22b, -Stx5, -VAMP3 and -VAMP8 were obtained from Synaptic Systems; anti-β-actin, -ERGIC53 and -microtubule-associated protein 1 light chain 3 (LC3B) from Sigma; anti-PDI and -CNX from Enzo Life Sciences; anti-CRT from ThermoFisher; anti-glucose-regulated protein 78 (GRP78/BiP) from BD Signalling; and anti-Sec23 and T-cell immune regulator 1 (TCIRG1) from Abcam.

    Techniques: Infection, Western Blot, Marker, Immunofluorescence, Microscopy, Immuno-Electron Microscopy, Staining