Structured Review

GenScript synthetic double stranded dna fragments
Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and <t>V5</t> epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) <t>DNA</t> samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .
Synthetic Double Stranded Dna Fragments, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synthetic double stranded dna fragments/product/GenScript
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
synthetic double stranded dna fragments - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells"

Article Title: VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

Journal: eLife

doi: 10.7554/eLife.36654

Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and V5 epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) DNA samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .
Figure Legend Snippet: Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and V5 epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) DNA samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .

Techniques Used: Western Blot, SDS Page, Purification, Isolation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining

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    GenScript synthetic double stranded dna fragments
    Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and <t>V5</t> epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) <t>DNA</t> samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .
    Synthetic Double Stranded Dna Fragments, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synthetic double stranded dna fragments/product/GenScript
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    synthetic double stranded dna fragments - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    84
    GenScript dsdna parts
    Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and <t>V5</t> epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) <t>DNA</t> samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .
    Dsdna Parts, supplied by GenScript, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsdna parts/product/GenScript
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dsdna parts - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

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    Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and V5 epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) DNA samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .

    Journal: eLife

    Article Title: VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

    doi: 10.7554/eLife.36654

    Figure Lengend Snippet: Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and V5 epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) DNA samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .

    Article Snippet: For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments (GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit (New England Biolabs ), creating pEL558-pEL561.

    Techniques: Western Blot, SDS Page, Purification, Isolation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining