Synthetic Double Stranded Dna Fragments, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells"
Article Title: VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells
Figure Legend Snippet: Control experiments for yeast VivosX. ( A,B ) Immunoblots showing the different effects of the FLAG and V5 epitope tags on the SDS-PAGE mobility of the H2A.Z and H2A histones. ( C ) DNA samples purified from chromatin isolated from the indicated strains before and after MNase digestion were analyzed by agarose gel electrophoresis and SYBR Green staining. 1x MNase represents 0.08 U/µL of MNase. Circled lanes: MNase digestion conditions selected for the experiment in Figure 3D . ( D,E ) Logarithmically growing HTZ1(T46C) FL cells were fixed with sodium azide (0.1%) for 15 min on ice before treatment with and without 4-DPS (180 µM) for 20 min. The cells were then fixed with TCA fixation and the proteins extracted and analyzed as described for Figure 1D .
Techniques Used: Western Blot, SDS Page, Purification, Isolation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining