Journal: PLoS ONE

Article Title: Anti-Obesity Sodium Tungstate Treatment Triggers Axonal and Glial Plasticity in Hypothalamic Feeding Centers

doi: 10.1371/journal.pone.0039087

Figure Lengend Snippet: Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) HPC-1 show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.

Article Snippet: The sections were incubated overnight with primary antibodies: c-fos, syntaxin 1A (HPC-1) 1∶100 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); β-tubulin 1∶200, synaptosomal-associated protein 25 (SNAP-25) 1∶100, collapsing response mediator protein 2 (CRMP2) 1∶50 (Abcam, Cambridge, United Kingdom); glial fibrillary acidic protein (GFAP) 1∶100 (Sigma-Aldrich); and neurogenic differentiation factor 1 (NeuroD1) 1∶200 (Chemicon, Temecula, CA, USA).

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