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Santa Cruz Biotechnology synpo
Nphs2-, <t>Nphs1-</t> and <t>Synpo</t> staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
Synpo, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synpo/product/Santa Cruz Biotechnology
Average 80 stars, based on 8 article reviews
Price from $9.99 to $1999.99
synpo - by Bioz Stars, 2022-09
80/100 stars

Images

1) Product Images from "Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression"

Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

Journal: PLoS ONE

doi: 10.1371/journal.pone.0137043

Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
Figure Legend Snippet: Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

Techniques Used: Staining, Immunohistochemistry, Mouse Assay, Labeling

Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.
Figure Legend Snippet: Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

Techniques Used: Staining, Immunohistochemistry, Mouse Assay, Expressing

2) Product Images from "Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids"

Article Title: Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

Journal: Nature Communications

doi: 10.1038/ncomms9715

Tubular organoids recapitulate kidney development and architecture. ( a ) Phase contrast images of spheroid differentiation into tubular organoids. Red arrowheads highlight epithelia. ( b ) Confocal optical sections showing LTL with nephron progenitor markers Sine oculis homeobox homologue 2 (SIX2), Lin11-Isl1-Mec3 (LIM) homeobox 1 (LHX1), paired box gene 2 (PAX2) and ( c ) proximal tubule markers low density lipoprotein-related protein 2/megalin and cubilin in tubular organoids. ( d ) Electron micrographs of a representative tubule, with progressive magnifications of regions in coloured boxes highlighting apical microvilli (arrowheads) and tight junctions (arrows). ( e ) Wide-field images showing tubule anatomical progression from E-cadherin (ECAD) + to LTL + to PODXL + organoid segments. ( f ) Low-magnification image of organoid with interlacing tubules and peripheral PODXL + aggregates (left) and high-magnification confocal optical section showing co-localization of synaptopodin (SYNPO) and Wilms tumour protein (WT1) in organoid podocyte-like cells (right). ( g ) Wide-field immunofluorescence showing co-localization of CD31 with von Willebrand factor (vWF, left), or with nephron markers in tubular organoids derived from hESCs and iPSCs (right). White arrowheads show interactions between tubular, podocyte-like and endothelial compartments. White dashed outline highlights a representative tubular terminus. Images are representative of one hESC line and three iPSC lines from different patients. ( h ) Number of tubular organoids formed per unit surface area in cultures of hESCs and iPSCs (left) and per cent of these LTL + organoids associated with CD31 + and PODXL + cell types within the organoid (right). ( i ) Confocal images of organoid-derived human tubule (H) with LTL reactivity after 3 weeks of growth inside the developing mouse kidney cortex (m). Scale bars, 100 μm. Error bars, s.e.m ( n ≥3 experiments).
Figure Legend Snippet: Tubular organoids recapitulate kidney development and architecture. ( a ) Phase contrast images of spheroid differentiation into tubular organoids. Red arrowheads highlight epithelia. ( b ) Confocal optical sections showing LTL with nephron progenitor markers Sine oculis homeobox homologue 2 (SIX2), Lin11-Isl1-Mec3 (LIM) homeobox 1 (LHX1), paired box gene 2 (PAX2) and ( c ) proximal tubule markers low density lipoprotein-related protein 2/megalin and cubilin in tubular organoids. ( d ) Electron micrographs of a representative tubule, with progressive magnifications of regions in coloured boxes highlighting apical microvilli (arrowheads) and tight junctions (arrows). ( e ) Wide-field images showing tubule anatomical progression from E-cadherin (ECAD) + to LTL + to PODXL + organoid segments. ( f ) Low-magnification image of organoid with interlacing tubules and peripheral PODXL + aggregates (left) and high-magnification confocal optical section showing co-localization of synaptopodin (SYNPO) and Wilms tumour protein (WT1) in organoid podocyte-like cells (right). ( g ) Wide-field immunofluorescence showing co-localization of CD31 with von Willebrand factor (vWF, left), or with nephron markers in tubular organoids derived from hESCs and iPSCs (right). White arrowheads show interactions between tubular, podocyte-like and endothelial compartments. White dashed outline highlights a representative tubular terminus. Images are representative of one hESC line and three iPSC lines from different patients. ( h ) Number of tubular organoids formed per unit surface area in cultures of hESCs and iPSCs (left) and per cent of these LTL + organoids associated with CD31 + and PODXL + cell types within the organoid (right). ( i ) Confocal images of organoid-derived human tubule (H) with LTL reactivity after 3 weeks of growth inside the developing mouse kidney cortex (m). Scale bars, 100 μm. Error bars, s.e.m ( n ≥3 experiments).

Techniques Used: Immunofluorescence, Derivative Assay

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    Santa Cruz Biotechnology synaptopodin
    Effects of conditioned MSCs on expression of <t>synaptopodin</t> and fibronectin in podocytes. Podocytes were incubated without or with 15 ng/ml TGF-β1, conditioned MSCs and/or ascorbic acid for 72 hrs. Cell lysates were analysed with Western blotting. ( A ) Representative Western blot and bar graph analysis of relative protein level of synaptopodin, which were normalized to control. ( B ) Bar graph analysis of quantitative PCR analysis of relative fibronectin expression to GAPDH normalized to control. * P
    Synaptopodin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of conditioned MSCs on expression of synaptopodin and fibronectin in podocytes. Podocytes were incubated without or with 15 ng/ml TGF-β1, conditioned MSCs and/or ascorbic acid for 72 hrs. Cell lysates were analysed with Western blotting. ( A ) Representative Western blot and bar graph analysis of relative protein level of synaptopodin, which were normalized to control. ( B ) Bar graph analysis of quantitative PCR analysis of relative fibronectin expression to GAPDH normalized to control. * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Conditioned mesenchymal stem cells attenuate progression of chronic kidney disease through inhibition of epithelial-to-mesenchymal transition and immune modulation

    doi: 10.1111/j.1582-4934.2012.01610.x

    Figure Lengend Snippet: Effects of conditioned MSCs on expression of synaptopodin and fibronectin in podocytes. Podocytes were incubated without or with 15 ng/ml TGF-β1, conditioned MSCs and/or ascorbic acid for 72 hrs. Cell lysates were analysed with Western blotting. ( A ) Representative Western blot and bar graph analysis of relative protein level of synaptopodin, which were normalized to control. ( B ) Bar graph analysis of quantitative PCR analysis of relative fibronectin expression to GAPDH normalized to control. * P

    Article Snippet: The membrane was incubated with 1A4 anti-α- SMA (Clone1A4, 1:1000 dilution; Sigma-Aldrich), fibronectin (H-300, 1:400 dilution; Santa Cruz, CA, USA) or synaptopodin (H-140, 1:1000 dilution; Santa Cruz) mAb.

    Techniques: Expressing, Incubation, Western Blot, Real-time Polymerase Chain Reaction