sybrgreen polymerase chain reaction pcr mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sybrgreen polymerase chain reaction pcr mix
    Sybrgreen Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen polymerase chain reaction pcr mix/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sybrgreen polymerase chain reaction pcr mix - by Bioz Stars, 2020-08
    92/100 stars

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    Polymerase Chain Reaction:

    Article Title: Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors
    Article Snippet: .. The fluorescent SYBRGreen polymerase chain reaction (PCR) mix (Thermo Scientific) was used to quantify the PCR products. .. Specific primers for the genes of interest (insulin-like growth factor 1 receptor [IGF-1R], FSHR, luteotropic hormone receptor [LHR], and CYP19A1) were added, and a final volume of 10 ml was used for the PCR step. β-Actin was used as a reference (normalization) gene for each sample.

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    Thermo Fisher total rna
    <t>RNA-seq</t> after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c – f Volcano plots of fold change vs p -value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c , MAB-MiPs untreated vs MAB-MiPs+PMC d , fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e , fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f . Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from <t>ChIP-qPCR</t> experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA ( n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 277 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher fast sybrgreen master mix
    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and <t>SybrGreen</t> PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.
    Fast Sybrgreen Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybrgreen master mix/product/Thermo Fisher
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    fast sybrgreen master mix - by Bioz Stars, 2020-08
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    93
    Thermo Fisher sybrgreen master mix
    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and <t>SybrGreen</t> PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.
    Sybrgreen Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen master mix/product/Thermo Fisher
    Average 93 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    sybrgreen master mix - by Bioz Stars, 2020-08
    93/100 stars
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    RNA-seq after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c – f Volcano plots of fold change vs p -value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c , MAB-MiPs untreated vs MAB-MiPs+PMC d , fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e , fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f . Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from ChIP-qPCR experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA ( n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)

    Journal: Nature Communications

    Article Title: MicroRNAs promote skeletal muscle differentiation of mesodermal iPSC-derived progenitors

    doi: 10.1038/s41467-017-01359-w

    Figure Lengend Snippet: RNA-seq after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c – f Volcano plots of fold change vs p -value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c , MAB-MiPs untreated vs MAB-MiPs+PMC d , fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e , fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f . Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from ChIP-qPCR experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA ( n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)

    Article Snippet: Gene qPCR was performed on 1:5 diluted cDNA obtained from 1 μg total RNA (SybrGreen mix, SSIII cDNA production kit and RNA extraction kit from Thermo Fisher Scientific), using Viia7 384-plate reader (Thermo Fisher Scientific; final primer concentration, 100 nM; final volume, 10 μl; PGK , internal reference; thermal profile, 95 °C 15 s, 60 °C 60 s, 40×). miRNA qPCR was performed on 1:15 diluted cDNA obtained from 20 ng total miRNA preparation (miRNA isolation kit, Thermo Fisher Scientific).

    Techniques: RNA Sequencing Assay, Quantitation Assay, Methylation Sequencing, Methylation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Marker, Derivative Assay

    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction