sybrgreen pcr master mix  (Thermo Fisher)


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    Name:
    PCR Master Mix 2X
    Description:
    Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase dNTPs and all of the components required for PCR except DNA template and primers This pre mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up The mix is optimized for efficient and reproducible PCR Highlights• Convenient ready to use mix• Thermostable half life is more than 40 min at 95°C• Generates PCR products with 3 dA overhangs• Incorporates modified nucleotides e g biotin digoxigenin fluorescently labeled nucleotides Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate of Taq DNA Polymerase in PCR is 2 2 x 10 5 errors per nt per cycle as determined by a modified method described in Accordingly the accuracy of PCR is 4 5 x 104 Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
    Catalog Number:
    k0171
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|Routine PCR
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher sybrgreen pcr master mix
    HPV <t>SybrGreen</t> <t>PCR</t> and differentials of association curves of PCR products. A: Real-time SybrGreen amplification patterns of four SiHa cell line dilutions corresponding to an input of 50, 25, 12, and 2 HPV genomes ( A ) and a selection of 6 of the 25 ovarian cancer samples. The four SiHa cell line dilutions and 3 of the 25 ovarian cancer cases passed the threshold ( B ). For reasons of clarity, only 5 of the 22 other ovarian cancer samples not passing the threshold are depicted ( C ). A (mock tissue) negative control ( D ) is also shown. B: The differentials of the association curves of the samples in A (identical labeling patterns). With two exceptions, the low-copy number HPV input sample and one of the three ovarian cancer samples that surpassed the SybrGreen threshold, the curves can be placed in three clusters: one with a peak around 80°C (representing fragmented high-molecular weight DNA that smears higher up on a gel), one with a peak around 73°C (representing HPV amplicons that give a distinct band on gel), and one with a peak around 63°C (representing fragmented low-molecular weight DNA and/or primer dimers that run low in the gel).
    Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase dNTPs and all of the components required for PCR except DNA template and primers This pre mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up The mix is optimized for efficient and reproducible PCR Highlights• Convenient ready to use mix• Thermostable half life is more than 40 min at 95°C• Generates PCR products with 3 dA overhangs• Incorporates modified nucleotides e g biotin digoxigenin fluorescently labeled nucleotides Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate of Taq DNA Polymerase in PCR is 2 2 x 10 5 errors per nt per cycle as determined by a modified method described in Accordingly the accuracy of PCR is 4 5 x 104 Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
    https://www.bioz.com/result/sybrgreen pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 213 article reviews
    Price from $9.99 to $1999.99
    sybrgreen pcr master mix - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction"

    Article Title: A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction

    Journal: The American Journal of Pathology

    doi:

    HPV SybrGreen PCR and differentials of association curves of PCR products. A: Real-time SybrGreen amplification patterns of four SiHa cell line dilutions corresponding to an input of 50, 25, 12, and 2 HPV genomes ( A ) and a selection of 6 of the 25 ovarian cancer samples. The four SiHa cell line dilutions and 3 of the 25 ovarian cancer cases passed the threshold ( B ). For reasons of clarity, only 5 of the 22 other ovarian cancer samples not passing the threshold are depicted ( C ). A (mock tissue) negative control ( D ) is also shown. B: The differentials of the association curves of the samples in A (identical labeling patterns). With two exceptions, the low-copy number HPV input sample and one of the three ovarian cancer samples that surpassed the SybrGreen threshold, the curves can be placed in three clusters: one with a peak around 80°C (representing fragmented high-molecular weight DNA that smears higher up on a gel), one with a peak around 73°C (representing HPV amplicons that give a distinct band on gel), and one with a peak around 63°C (representing fragmented low-molecular weight DNA and/or primer dimers that run low in the gel).
    Figure Legend Snippet: HPV SybrGreen PCR and differentials of association curves of PCR products. A: Real-time SybrGreen amplification patterns of four SiHa cell line dilutions corresponding to an input of 50, 25, 12, and 2 HPV genomes ( A ) and a selection of 6 of the 25 ovarian cancer samples. The four SiHa cell line dilutions and 3 of the 25 ovarian cancer cases passed the threshold ( B ). For reasons of clarity, only 5 of the 22 other ovarian cancer samples not passing the threshold are depicted ( C ). A (mock tissue) negative control ( D ) is also shown. B: The differentials of the association curves of the samples in A (identical labeling patterns). With two exceptions, the low-copy number HPV input sample and one of the three ovarian cancer samples that surpassed the SybrGreen threshold, the curves can be placed in three clusters: one with a peak around 80°C (representing fragmented high-molecular weight DNA that smears higher up on a gel), one with a peak around 73°C (representing HPV amplicons that give a distinct band on gel), and one with a peak around 63°C (representing fragmented low-molecular weight DNA and/or primer dimers that run low in the gel).

    Techniques Used: Polymerase Chain Reaction, Amplification, Selection, Negative Control, Labeling, Low Copy Number, Molecular Weight

    Comparison of real-time HPV PCR amplification reported by molecular beacons and SybrGreen. A selection of three samples from cervical cancer patients and a negative control (mock tissue section) is shown. HPV molecular beacon PCR ( filled symbols ): the three patient samples were genotyped as HPV-16 ( squares ), HPV-45 ( triangles ), and HPV-18 ( circles ). The mock tissue was negative with all seven HPV molecular beacons ( diamonds ). HPV SybrGreen PCR ( open symbols ): all three patient samples reported positive, whereas the negative control did not. The dashed line represents the threshold.
    Figure Legend Snippet: Comparison of real-time HPV PCR amplification reported by molecular beacons and SybrGreen. A selection of three samples from cervical cancer patients and a negative control (mock tissue section) is shown. HPV molecular beacon PCR ( filled symbols ): the three patient samples were genotyped as HPV-16 ( squares ), HPV-45 ( triangles ), and HPV-18 ( circles ). The mock tissue was negative with all seven HPV molecular beacons ( diamonds ). HPV SybrGreen PCR ( open symbols ): all three patient samples reported positive, whereas the negative control did not. The dashed line represents the threshold.

    Techniques Used: Polymerase Chain Reaction, Amplification, Selection, Negative Control

    Ct values of the β-globin molecular beacon PCR ( closed circles ) and the HPV-CPI/IIG-SybrGreen PCR ( open squares ) as a function of the amount of input SiHa cell DNA. SiHa carries one to two copies of HPV-16. Bars indicate standard errors of three independent experiments, the range of standard errors: 0.1 to 0.76 cycles. With the ΔCt approach accurate quantification of viral over nuclear genome copy number is possible even with 20 cell equivalents.
    Figure Legend Snippet: Ct values of the β-globin molecular beacon PCR ( closed circles ) and the HPV-CPI/IIG-SybrGreen PCR ( open squares ) as a function of the amount of input SiHa cell DNA. SiHa carries one to two copies of HPV-16. Bars indicate standard errors of three independent experiments, the range of standard errors: 0.1 to 0.76 cycles. With the ΔCt approach accurate quantification of viral over nuclear genome copy number is possible even with 20 cell equivalents.

    Techniques Used: Polymerase Chain Reaction

    Related Articles

    Amplification:

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Article Title: Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia
    Article Snippet: .. For realtime PCR, reverse transcribed material corresponding to 40 ng RNA was amplified with the TaqMan assays described below in 25 μl Universal PCR Master Mix, No AmpErase UNG on the SDS 7000 (Applied Biosystems) using the standard thermal protocol. ..

    Polymerase Chain Reaction:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Article Title: The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo
    Article Snippet: .. To screen samples for presence of the Tam-Cre transgene, PCR master mix was prepared to a final concentration of 0.2 mM dNTP (ThermoScientific Fermentas R0192), 2 mM MgCl2 and 0.625 units Platinum® Taq DNA polymerase (Invitrogen 10966-034) and 0.5 μM each of specified primers (Ventura et al. ). .. Samples were incubated at 94 °C for 180 s, followed by 35 cycles of 94 °C for 30 s, 58 °C for 90 s and 72 °C for 60 s. Samples were held at a final extension temperature of 72 °C for 300 s. For iFat1 reactions, PCR master mix was composed of 0.3 mM dNTP, 10× PCR buffer, 1.5 mM MgCl2 and 1.5 units of Platinum ® Taq and 0.4 μM each of: 5′-ACGTCAGTAGTCATAGGAACTGCGGTCG-3′(F) and 5′-CCAACCGGTGGGACATTT GAGTTG-3′(R).

    Article Title: Regulator of G protein signaling 2 is a functionally important negative regulator of angiotensin II-induced cardiac fibroblast responses
    Article Snippet: .. Reverse-transcribed (TaqMan reverse transcription reagents) RNA samples from fibroblasts were subjected to real-time PCR using FAM-labeled TaqMan probes for RGS2, RGS3, RGS5, GAPDH, and 18S and universal PCR master mix according to the manufacturer's instructions (Applied Biosystems, Carlsbad, CA). .. Each sample was assayed in duplicate in two independent PCR reactions and normalized to 18S expression.

    Article Title: Characterization of the transcripts and protein isoforms for cytoplasmic polyadenylation element binding protein-3 (CPEB3) in the mouse retina
    Article Snippet: .. PCR master mix containing Taq DNA polymerase, dNTPs and optimized reaction buffer were also obtained from Applied Biosystems. .. PCR reactions were performed with an ABI 7300 real-time PCR system using the following conditions: 50°C, 2 min; 95°C, 10 min; 40 cycles of (95°C, 15 sec; 60°C, 1 min).

    Article Title: Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia
    Article Snippet: .. For realtime PCR, reverse transcribed material corresponding to 40 ng RNA was amplified with the TaqMan assays described below in 25 μl Universal PCR Master Mix, No AmpErase UNG on the SDS 7000 (Applied Biosystems) using the standard thermal protocol. ..

    Article Title: ING1b-inducible microRNA203 inhibits cell proliferation
    Article Snippet: .. The TaqMan Array Human MicroRNA Card Set v3.0, TaqMan MicroRNA Reverse Transcription Kit, the primers for miRNAs and U6B and universal PCR Master Mix were purchased from Applied Biosystems (Foster City, CA, USA). .. MicroRNA mimics and MicroRNA mimic controls were purchased from Dharmacon (Lafayette, CO, USA).

    Sequencing:

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Article Title: Regulator of G protein signaling 2 is a functionally important negative regulator of angiotensin II-induced cardiac fibroblast responses
    Article Snippet: .. Reverse-transcribed (TaqMan reverse transcription reagents) RNA samples from fibroblasts were subjected to real-time PCR using FAM-labeled TaqMan probes for RGS2, RGS3, RGS5, GAPDH, and 18S and universal PCR master mix according to the manufacturer's instructions (Applied Biosystems, Carlsbad, CA). .. Each sample was assayed in duplicate in two independent PCR reactions and normalized to 18S expression.

    Concentration Assay:

    Article Title: The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo
    Article Snippet: .. To screen samples for presence of the Tam-Cre transgene, PCR master mix was prepared to a final concentration of 0.2 mM dNTP (ThermoScientific Fermentas R0192), 2 mM MgCl2 and 0.625 units Platinum® Taq DNA polymerase (Invitrogen 10966-034) and 0.5 μM each of specified primers (Ventura et al. ). .. Samples were incubated at 94 °C for 180 s, followed by 35 cycles of 94 °C for 30 s, 58 °C for 90 s and 72 °C for 60 s. Samples were held at a final extension temperature of 72 °C for 300 s. For iFat1 reactions, PCR master mix was composed of 0.3 mM dNTP, 10× PCR buffer, 1.5 mM MgCl2 and 1.5 units of Platinum ® Taq and 0.4 μM each of: 5′-ACGTCAGTAGTCATAGGAACTGCGGTCG-3′(F) and 5′-CCAACCGGTGGGACATTT GAGTTG-3′(R).

    Expressing:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Tumor Suppressor Role for the SPOP Ubiquitin Ligase in Signal-Dependent Proteolysis of the Oncogenic Coactivator SRC-3/AIB1
    Article Snippet: .. Each TaqMan probe for its corresponding target gene mRNA and One-Step RT-PCR master mix also were from Applied Biosystems. .. Soft Agar Assay This assay was performed according to Cell Transformation Detection Assay (Chemicon International).

    Chromatin Immunoprecipitation:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

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    Thermo Fisher fast sybrgreen master mix
    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and <t>SybrGreen</t> PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.
    Fast Sybrgreen Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybrgreen master mix/product/Thermo Fisher
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    fast sybrgreen master mix - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Thermo Fisher master mix maxima sybrgreen qpcr mm
    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and <t>SybrGreen</t> PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.
    Master Mix Maxima Sybrgreen Qpcr Mm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/master mix maxima sybrgreen qpcr mm/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    master mix maxima sybrgreen qpcr mm - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction