sybrgreen pcr master kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    SYBR Green PCR Master Mix
    Description:
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis Everything you need for SYBR Green dye based PCR amplification and detection in a convenient single tube format Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye AmpliTaq Gold DNA Polymerase dNTPs with dUTP Passive Reference 1 and optimized buffer in the convenience of a single vial • Premixed components stored at 2 8°C significantly reduce assay setup time• SYBR Green I dye detects double stranded DNA so specific probes are not required• AmpliTaq Gold DNA polymerase minimizes nonspecific product formation to achieve superior performance• dUTP significantly reduces carryover contamination when used in conjunction with uracil DNA glycosylase• Proprietary buffer enhancements ensure performance and reliabilityMaximum Flexibility and ConvenienceApplied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target specific TaqMan probes are required SYBR Green I dye is a double stranded DNA binding dye that detects any double stranded DNA generated during PCR The hot start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation including primer dimers yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non PCR related fluorescence fluctuations This minimizes well to well variability that can result from a variety of causes such as pipetting error or sample evaporation SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed
    Catalog Number:
    4309155
    Price:
    None
    Applications:
    Enzymes & Master Mixes for Real-Time PCR|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher sybrgreen pcr master kit
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis Everything you need for SYBR Green dye based PCR amplification and detection in a convenient single tube format Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye AmpliTaq Gold DNA Polymerase dNTPs with dUTP Passive Reference 1 and optimized buffer in the convenience of a single vial • Premixed components stored at 2 8°C significantly reduce assay setup time• SYBR Green I dye detects double stranded DNA so specific probes are not required• AmpliTaq Gold DNA polymerase minimizes nonspecific product formation to achieve superior performance• dUTP significantly reduces carryover contamination when used in conjunction with uracil DNA glycosylase• Proprietary buffer enhancements ensure performance and reliabilityMaximum Flexibility and ConvenienceApplied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target specific TaqMan probes are required SYBR Green I dye is a double stranded DNA binding dye that detects any double stranded DNA generated during PCR The hot start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation including primer dimers yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non PCR related fluorescence fluctuations This minimizes well to well variability that can result from a variety of causes such as pipetting error or sample evaporation SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed
    https://www.bioz.com/result/sybrgreen pcr master kit/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    sybrgreen pcr master kit - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    amplitaq gold polymerase
    icycler
    cdna
    qpcr
    bio-rad cfx

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix Applied Biosystems). ..

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System. ..

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology
    Article Snippet: .. 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix. .. For the SYBR green PCR assay, the 20 μl PCR mix reaction mix was comprised of 5 μl of cDNA and 15 μl of master mix prepared using 1 μl of the 10 pmol forward and reverse primers, 10 μl SYBR® Green I and 3 μl of nuclease free water.

    Amplification:

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
    Article Snippet: .. An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA). .. The sample target RNA copy numbers were calculated using SDS 1.9 Software (Applied Biosystems, Foster City, CA, USA).

    Article Title: RNase L restricts the mobility of engineered retrotransposons in cultured human cells
    Article Snippet: .. The resultant cDNA was amplified using Sybr Green PCR master mix (Gibco/Life Technologies) on a StepOnePlus system according to manufacturer’s protocol. ..

    In Vitro:

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology
    Article Snippet: .. 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix. .. For the SYBR green PCR assay, the 20 μl PCR mix reaction mix was comprised of 5 μl of cDNA and 15 μl of master mix prepared using 1 μl of the 10 pmol forward and reverse primers, 10 μl SYBR® Green I and 3 μl of nuclease free water.

    Sequencing:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Quantitative RT-PCR:

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology
    Article Snippet: .. 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix. .. For the SYBR green PCR assay, the 20 μl PCR mix reaction mix was comprised of 5 μl of cDNA and 15 μl of master mix prepared using 1 μl of the 10 pmol forward and reverse primers, 10 μl SYBR® Green I and 3 μl of nuclease free water.

    SYBR Green Assay:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix Applied Biosystems). ..

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
    Article Snippet: .. An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA). .. The sample target RNA copy numbers were calculated using SDS 1.9 Software (Applied Biosystems, Foster City, CA, USA).

    Article Title: RNase L restricts the mobility of engineered retrotransposons in cultured human cells
    Article Snippet: .. The resultant cDNA was amplified using Sybr Green PCR master mix (Gibco/Life Technologies) on a StepOnePlus system according to manufacturer’s protocol. ..

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System. ..

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology
    Article Snippet: .. 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix. .. For the SYBR green PCR assay, the 20 μl PCR mix reaction mix was comprised of 5 μl of cDNA and 15 μl of master mix prepared using 1 μl of the 10 pmol forward and reverse primers, 10 μl SYBR® Green I and 3 μl of nuclease free water.

    Article Title: hCAF1/CNOT7 regulates interferon signalling by targeting STAT1
    Article Snippet: .. After DNA purification, qPCRs were performed in triplicate in a 96-well optical reaction plate using SYBR Green PCR Master Mix (Life Technology). .. The –ΔΔCt values for each locus were calculated with respect to the ChIP input DNA, normalized to a reference locus (3′ downstream region of the GAPDH gene).

    Immunoprecipitation:

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    DNA Purification:

    Article Title: hCAF1/CNOT7 regulates interferon signalling by targeting STAT1
    Article Snippet: .. After DNA purification, qPCRs were performed in triplicate in a 96-well optical reaction plate using SYBR Green PCR Master Mix (Life Technology). .. The –ΔΔCt values for each locus were calculated with respect to the ChIP input DNA, normalized to a reference locus (3′ downstream region of the GAPDH gene).

    Polymerase Chain Reaction:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix Applied Biosystems). ..

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
    Article Snippet: .. An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA). .. The sample target RNA copy numbers were calculated using SDS 1.9 Software (Applied Biosystems, Foster City, CA, USA).

    Article Title: RNase L restricts the mobility of engineered retrotransposons in cultured human cells
    Article Snippet: .. The resultant cDNA was amplified using Sybr Green PCR master mix (Gibco/Life Technologies) on a StepOnePlus system according to manufacturer’s protocol. ..

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System. ..

    Article Title: hCAF1/CNOT7 regulates interferon signalling by targeting STAT1
    Article Snippet: .. After DNA purification, qPCRs were performed in triplicate in a 96-well optical reaction plate using SYBR Green PCR Master Mix (Life Technology). .. The –ΔΔCt values for each locus were calculated with respect to the ChIP input DNA, normalized to a reference locus (3′ downstream region of the GAPDH gene).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher fast sybrgreen master mix
    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and <t>SybrGreen</t> PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.
    Fast Sybrgreen Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybrgreen master mix/product/Thermo Fisher
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    fast sybrgreen master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher powerup sybrgreen master mix
    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and <t>SybrGreen</t> PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.
    Powerup Sybrgreen Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powerup sybrgreen master mix/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    powerup sybrgreen master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction