dnase i  (New England Biolabs)


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  • 99
    Name:
    DNase I RNase free
    Description:
    DNase I RNase free 5 000 units
    Catalog Number:
    m0303l
    Price:
    282
    Size:
    5 000 units
    Category:
    Deoxyribonucleases DNase
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    Structured Review

    New England Biolabs dnase i
    DNase I RNase free
    DNase I RNase free 5 000 units
    https://www.bioz.com/result/dnase i/product/New England Biolabs
    Average 99 stars, based on 1246 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Stability Assay:

    Article Title: In vitro selection of an XNA aptamer capable of small-molecule recognition
    Article Snippet: .. TNA stability assay FAM-labeled DNA aptamer A08 and TNA aptamer A04T.2 were prepared in three nuclease challenge conditions: 1.5 U DNase I (RNase-free, New England Biolabs) in 1× DNase I reaction buffer (10 mM Tris–HCl, 2.5 mM MgCl2 , 0.5 mM CaCl2 , pH 7.6), 50% (v/v) human blood serum (normal pool, Fisher BioReagents) in 1× Dulbecco's PBS, 0.5 mg/ml pooled human liver microsomes (XenoTech) in 1× selection buffer. .. TNA and DNA aptamer (4 pmol) were each folded in the respective buffer for each condition and DNase I, human blood serum, and human liver microsomes were added to the desired concentration.

    Fluorescence:

    Article Title: In-Cell RNA Hydrolysis Assay: A Method for the Determination of the RNase Activity of Potential RNases
    Article Snippet: .. The fluorescence intensity of RiboGreen was similar in the presence and absence of DNase I (Fig. f). .. DNA molecules packaged into chromatin in the nucleus would not be attacked by a protein with DNA- and RNA-hydrolyzing activities, such as 3D8 scFv antibody; therefore, the majority of RiboGreen fluorescence in this assay can be attributed to RNA, not to DNA.

    Article Title: In-Cell RNA Hydrolysis Assay: A Method for the Determination of the RNase Activity of Potential RNases
    Article Snippet: .. The fluorescence intensity of RiboGreen in the conditioned medium of cells treated with RNase A or 3D8 scFv antibody was similar in the presence and absence of DNase I. ..

    Positive Control:

    Article Title: Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not
    Article Snippet: .. Bovine DNase I (New England Biolabs) was used as positive control. .. The reaction was stopped by a 15 min incubation with 2 mg/mL of proteinase K and 2% SDS.

    Lambda DNA Preparation:

    Article Title: Multipronged regulatory functions of a novel endonuclease (TieA) from Helicobacter pylori
    Article Snippet: .. TieA showed a non-specific endonuclease activity similar to DNase I as it cleaved both circular pUC19 and linearized lambda DNA, whereas Rv3131 (lane 6) as well as heat-inactivated TieA (lane 4) did not show any detectable DNA cleavage activity (Figure ). .. Furthermore, our results demonstrated a Ca2+ –Mg2+ dependent DNA cleavage activity of TieA, since EDTA and SDS markedly inhibited the cleavage activity of TieA (Figure , lanes 8, 9, 17, 18).

    Purification:

    Article Title: The Chromatin Structure of the Long Control Region of Human Papillomavirus Type 16 Represses Viral Oncoprotein Expression
    Article Snippet: .. DNase I-treated DNA was purified by standard procedures and used as a template for the extension reaction with Vent (exo-) polymerase (New England Biolabs). .. The primer (5′-GATCGCAGATCTCGAGCCCGGGCTAGCACG-3′) was derived from the luciferase vector.

    Incubation:

    Article Title: Evidence that Altered Cis Element Spacing Affects PpsR Mediated Redox Control of Photosynthesis Gene Expression in Rubrivivax gelatinosus
    Article Snippet: .. Initial binding reaction mixtures were incubated for 30 min at 22°C followed by a 15 min DNase I digestion that was initiated by adding 3 μl of DNase I (New England Biolabs) at an approximately 1:100 dilution (0.02 units/μl) in a footprint binding buffer, which gave partial probe digestion. ..

    Selection:

    Article Title: In vitro selection of an XNA aptamer capable of small-molecule recognition
    Article Snippet: .. TNA stability assay FAM-labeled DNA aptamer A08 and TNA aptamer A04T.2 were prepared in three nuclease challenge conditions: 1.5 U DNase I (RNase-free, New England Biolabs) in 1× DNase I reaction buffer (10 mM Tris–HCl, 2.5 mM MgCl2 , 0.5 mM CaCl2 , pH 7.6), 50% (v/v) human blood serum (normal pool, Fisher BioReagents) in 1× Dulbecco's PBS, 0.5 mg/ml pooled human liver microsomes (XenoTech) in 1× selection buffer. .. TNA and DNA aptamer (4 pmol) were each folded in the respective buffer for each condition and DNase I, human blood serum, and human liver microsomes were added to the desired concentration.

    Activity Assay:

    Article Title: Multipronged regulatory functions of a novel endonuclease (TieA) from Helicobacter pylori
    Article Snippet: .. TieA showed a non-specific endonuclease activity similar to DNase I as it cleaved both circular pUC19 and linearized lambda DNA, whereas Rv3131 (lane 6) as well as heat-inactivated TieA (lane 4) did not show any detectable DNA cleavage activity (Figure ). .. Furthermore, our results demonstrated a Ca2+ –Mg2+ dependent DNA cleavage activity of TieA, since EDTA and SDS markedly inhibited the cleavage activity of TieA (Figure , lanes 8, 9, 17, 18).

    Binding Assay:

    Article Title: Evidence that Altered Cis Element Spacing Affects PpsR Mediated Redox Control of Photosynthesis Gene Expression in Rubrivivax gelatinosus
    Article Snippet: .. Initial binding reaction mixtures were incubated for 30 min at 22°C followed by a 15 min DNase I digestion that was initiated by adding 3 μl of DNase I (New England Biolabs) at an approximately 1:100 dilution (0.02 units/μl) in a footprint binding buffer, which gave partial probe digestion. ..

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