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Lonza sybr stain
Sybr Stain, supplied by Lonza, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sybr stain/product/Lonza
Average 80 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sybr stain - by Bioz Stars, 2020-01
80/100 stars

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Combined Bisulfite Restriction Analysis Assay:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: Paragraph title: ALU-Combined Bisulfite Restriction Analysis (COBRA) ... The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA).

Amplification:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: ALU-Combined Bisulfite Restriction Analysis (COBRA) To observe methylation levels of Alu in samples, the sodium-bisulfite-treated DNA in each sample was amplified by PCR containing 1x PCR buffer (Qiagen, Germany), 0.2 mM of deoxynucleotide triphosphate (Promega, USA), 1 mM of magnesium chloride (Qiagen, Germany), 25 U of HotStarTaq DNA Polymerase (Qiagen, Germany), and 0.3 μM primer pairs: ALU-BRev (5′-CTAACTTTTTATATTTTTAATAAAAACRAAATTTCAC CA-3′) where R = A and G and Y = C and T. For Alu amplification, the program was set as follows: 95 °C for 15 min, 40 cycles of 95 °C for 45 s, 57 °C for 45 s, and 72 °C for 45 s, followed by a final extension of 72 °C for 7 min [ ]. .. The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA).

Methylation:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: ALU-Combined Bisulfite Restriction Analysis (COBRA) To observe methylation levels of Alu in samples, the sodium-bisulfite-treated DNA in each sample was amplified by PCR containing 1x PCR buffer (Qiagen, Germany), 0.2 mM of deoxynucleotide triphosphate (Promega, USA), 1 mM of magnesium chloride (Qiagen, Germany), 25 U of HotStarTaq DNA Polymerase (Qiagen, Germany), and 0.3 μM primer pairs: ALU-BRev (5′-CTAACTTTTTATATTTTTAATAAAAACRAAATTTCAC CA-3′) where R = A and G and Y = C and T. For Alu amplification, the program was set as follows: 95 °C for 15 min, 40 cycles of 95 °C for 45 s, 57 °C for 45 s, and 72 °C for 45 s, followed by a final extension of 72 °C for 7 min [ ]. .. The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA).

Polymerase Chain Reaction:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: .. The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA). .. The band intensity of Alu methylation was observed and measured by typhoon fla 7000 and ImageQuanNT Software (Amersham biosciences, UK) (Additional file : Figure S1) [ ].

Incubation:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: Alu PCR products were subjected to COBRA using 2 U of TaqI (Thermo scientific, USA), 2 U of TasI (Thermo scientific, USA), 5x NEB3 buffer (New England Biolabs, USA), and 1 μg/ul bovine serum albumin (BSA) (New England Biolabs, USA) and incubated at 65 °C overnight. .. The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA).

Acrylamide Gel Assay:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: .. The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA). .. The band intensity of Alu methylation was observed and measured by typhoon fla 7000 and ImageQuanNT Software (Amersham biosciences, UK) (Additional file : Figure S1) [ ].

Staining:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: .. The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA). .. The band intensity of Alu methylation was observed and measured by typhoon fla 7000 and ImageQuanNT Software (Amersham biosciences, UK) (Additional file : Figure S1) [ ].

Software:

Article Title: The association between Alu hypomethylation and severity of type 2 diabetes mellitus
Article Snippet: The cut PCR products were analyzed by 8% acrylamide gel and SYBR stain (Lonza, USA). .. The band intensity of Alu methylation was observed and measured by typhoon fla 7000 and ImageQuanNT Software (Amersham biosciences, UK) (Additional file : Figure S1) [ ].

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    Lonza sybr green
    Sybr Green, supplied by Lonza, used in various techniques. Bioz Stars score: 84/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green/product/Lonza
    Average 84 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    sybr green - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    90
    Lonza sybr green i
    Allele-specific amplification and detection of the PCR products . ( A ) Schematic representation of the allele-specific primer PCR method. 'Koshihikari' (Ksh) allele specific primer forms a perfect match at the 3' end (SNP) with Ksh DNA sequence (1) but forms a mismatch with Kal DNA (2). 'Kasalath' (Kal) allele specific primer similarly forms a 3' end match with Kal DNA (4) and 3' end mismatch with Ksh DNA (3). Both allele specific primer has an artificial mismatch at third base from 3' end (blue circle) according to the result from Table 1. ( B ) Allele-specific amplification of SNP marker S0285 detected EtBr after gel electrophoresis. Fluorescence of same samples were detected with a UV transilluminator at room temperature (25°C) or immediately after heating to 80°C using <t>SYBR</t> Green I. Ksh allele-specific primers (lane 1 and 2) and Kal allele-specific primers (lane 3 and 4) were used for PCR of Ksh genomic DNA (lane 1 and 3) and Kal genomic DNA (lane 2 and 4). The specificity of the reaction is evident. ( C ) The effect of temperature on SYBR <t>Green</t> I fluorescence. The green line indicates the fluorescence intensity of the sample in which amplification had occurred, and the blue line shows the amplification-independent background fluorescence. The ratio of both (signal/noise ratio) is shown in red. ( D ) The relationship between the number of SNPs and samples in a single PCR operation using 96-well plate. In a single PCR operation, 48 samples SNP genotype could be examined (multi-sample, Fig. 2), or 48 locus SNPs of one sample could be examined (multi-locus, Fig. 3)
    Sybr Green I, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i/product/Lonza
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results


    Allele-specific amplification and detection of the PCR products . ( A ) Schematic representation of the allele-specific primer PCR method. 'Koshihikari' (Ksh) allele specific primer forms a perfect match at the 3' end (SNP) with Ksh DNA sequence (1) but forms a mismatch with Kal DNA (2). 'Kasalath' (Kal) allele specific primer similarly forms a 3' end match with Kal DNA (4) and 3' end mismatch with Ksh DNA (3). Both allele specific primer has an artificial mismatch at third base from 3' end (blue circle) according to the result from Table 1. ( B ) Allele-specific amplification of SNP marker S0285 detected EtBr after gel electrophoresis. Fluorescence of same samples were detected with a UV transilluminator at room temperature (25°C) or immediately after heating to 80°C using SYBR Green I. Ksh allele-specific primers (lane 1 and 2) and Kal allele-specific primers (lane 3 and 4) were used for PCR of Ksh genomic DNA (lane 1 and 3) and Kal genomic DNA (lane 2 and 4). The specificity of the reaction is evident. ( C ) The effect of temperature on SYBR Green I fluorescence. The green line indicates the fluorescence intensity of the sample in which amplification had occurred, and the blue line shows the amplification-independent background fluorescence. The ratio of both (signal/noise ratio) is shown in red. ( D ) The relationship between the number of SNPs and samples in a single PCR operation using 96-well plate. In a single PCR operation, 48 samples SNP genotype could be examined (multi-sample, Fig. 2), or 48 locus SNPs of one sample could be examined (multi-locus, Fig. 3)

    Journal: Plant Methods

    Article Title: Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species

    doi: 10.1186/1746-4811-6-12

    Figure Lengend Snippet: Allele-specific amplification and detection of the PCR products . ( A ) Schematic representation of the allele-specific primer PCR method. 'Koshihikari' (Ksh) allele specific primer forms a perfect match at the 3' end (SNP) with Ksh DNA sequence (1) but forms a mismatch with Kal DNA (2). 'Kasalath' (Kal) allele specific primer similarly forms a 3' end match with Kal DNA (4) and 3' end mismatch with Ksh DNA (3). Both allele specific primer has an artificial mismatch at third base from 3' end (blue circle) according to the result from Table 1. ( B ) Allele-specific amplification of SNP marker S0285 detected EtBr after gel electrophoresis. Fluorescence of same samples were detected with a UV transilluminator at room temperature (25°C) or immediately after heating to 80°C using SYBR Green I. Ksh allele-specific primers (lane 1 and 2) and Kal allele-specific primers (lane 3 and 4) were used for PCR of Ksh genomic DNA (lane 1 and 3) and Kal genomic DNA (lane 2 and 4). The specificity of the reaction is evident. ( C ) The effect of temperature on SYBR Green I fluorescence. The green line indicates the fluorescence intensity of the sample in which amplification had occurred, and the blue line shows the amplification-independent background fluorescence. The ratio of both (signal/noise ratio) is shown in red. ( D ) The relationship between the number of SNPs and samples in a single PCR operation using 96-well plate. In a single PCR operation, 48 samples SNP genotype could be examined (multi-sample, Fig. 2), or 48 locus SNPs of one sample could be examined (multi-locus, Fig. 3)

    Article Snippet: Add 2 μl 10 × SYBR Green I (catalogue No. 50513, Lonza, Basel, Switzerland) to the PCR product, and detect the fluorescence at 75°C.

    Techniques: Amplification, Polymerase Chain Reaction, Sequencing, Marker, Nucleic Acid Electrophoresis, Fluorescence, SYBR Green Assay