sybr select master mix  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    SYBR Select Master Mix
    Description:
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis SYBR Select Master Mix is a superior and cost effective solution for your real time PCR applications Features of the SYBR Select Master Mix include • Specific minimize primer dimer and non specific amplification• Reproducible and sensitive consistent amplification across a wide dynamic range• Bright contains SYBR GreenER dye for maximum brightness• Carry over contamination control contains heat labile UDG• Can be used in either standard or fast cycling modeFormulated for Maximum SpecificitySYBR Select Master Mix contains all the components needed for your real time PCR reaction except the template and primers in a convenient 2X concentration premix The master mix includes AmpliTaq DNA Polymerase UP a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity Obtain Reproducible Results Across a Wide Dynamic RangeSYBR Select Master Mix is specially formulated to provide robust results from 100 ng to 0 1 pg cDNA per reaction Figure 1 and can reliably detect a single copy of genomic DNA Figure 2 Contains SYBR GreenER Dye and Heat labile UDG• SYBR GreenER dye is less inhibitory to PCR than SYBR Green I dye resulting in brighter signals• Heat labile uracil DNA glycosylase UDG is included for worry free carryover contamination controlInstrument CompatibilitySYBR Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real time PCR instruments except the 7900HT real time PCR system It is also compatible with the Bio Rad IQ 5 Roche LightCycler LC480 and Stratagene MX3005P systems View performance data
    Catalog Number:
    4472903
    Price:
    None
    Applications:
    Enzymes & Master Mixes for Real-Time PCR|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher sybr select master mix
    Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using <t>SYBR</t> green technology <t>StepOne</t> RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis SYBR Select Master Mix is a superior and cost effective solution for your real time PCR applications Features of the SYBR Select Master Mix include • Specific minimize primer dimer and non specific amplification• Reproducible and sensitive consistent amplification across a wide dynamic range• Bright contains SYBR GreenER dye for maximum brightness• Carry over contamination control contains heat labile UDG• Can be used in either standard or fast cycling modeFormulated for Maximum SpecificitySYBR Select Master Mix contains all the components needed for your real time PCR reaction except the template and primers in a convenient 2X concentration premix The master mix includes AmpliTaq DNA Polymerase UP a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity Obtain Reproducible Results Across a Wide Dynamic RangeSYBR Select Master Mix is specially formulated to provide robust results from 100 ng to 0 1 pg cDNA per reaction Figure 1 and can reliably detect a single copy of genomic DNA Figure 2 Contains SYBR GreenER Dye and Heat labile UDG• SYBR GreenER dye is less inhibitory to PCR than SYBR Green I dye resulting in brighter signals• Heat labile uracil DNA glycosylase UDG is included for worry free carryover contamination controlInstrument CompatibilitySYBR Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real time PCR instruments except the 7900HT real time PCR system It is also compatible with the Bio Rad IQ 5 Roche LightCycler LC480 and Stratagene MX3005P systems View performance data
    https://www.bioz.com/result/sybr select master mix/product/Thermo Fisher
    Average 99 stars, based on 681 article reviews
    Price from $9.99 to $1999.99
    sybr select master mix - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles"

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19113515

    Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using SYBR green technology StepOne RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p
    Figure Legend Snippet: Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using SYBR green technology StepOne RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p

    Techniques Used: Expressing, Quantitative RT-PCR, SYBR Green Assay, Step One RT-PCR, Amplification

    2) Product Images from "Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles"

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19113515

    Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using SYBR green technology StepOne RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p
    Figure Legend Snippet: Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using SYBR green technology StepOne RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p

    Techniques Used: Expressing, Quantitative RT-PCR, SYBR Green Assay, Step One RT-PCR, Amplification

    3) Product Images from "Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1)"

    Article Title: Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1)

    Journal: Oncotarget

    doi: 10.18632/oncotarget.11720

    Loss of LASP1 in breast cancer cells affects gene expression of matrix metalloproteinases qRT-PCR in human MDA-MB-231-shLASP1 cells was performed with SYBR Select Master Mix. Bar graphs show the relative mRNA expression, calculated by the comparative ddCT-method and normalized to the housekeeping gene ribosomalprotein large P0 (RPLP0). LASP1 depletion decreased MMP1, MMP3, and MMP9 gene expression while MMP2 gene expression increased. MMP14 mRNA was not affected by LASP1 knockdown. *p
    Figure Legend Snippet: Loss of LASP1 in breast cancer cells affects gene expression of matrix metalloproteinases qRT-PCR in human MDA-MB-231-shLASP1 cells was performed with SYBR Select Master Mix. Bar graphs show the relative mRNA expression, calculated by the comparative ddCT-method and normalized to the housekeeping gene ribosomalprotein large P0 (RPLP0). LASP1 depletion decreased MMP1, MMP3, and MMP9 gene expression while MMP2 gene expression increased. MMP14 mRNA was not affected by LASP1 knockdown. *p

    Techniques Used: Expressing, Quantitative RT-PCR, Multiple Displacement Amplification

    4) Product Images from "Lipidomic analysis of cancer cells cultivated at acidic pH reveals phospholipid fatty acids remodelling associated with transcriptional reprogramming"

    Article Title: Lipidomic analysis of cancer cells cultivated at acidic pH reveals phospholipid fatty acids remodelling associated with transcriptional reprogramming

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    doi: 10.1080/14756366.2020.1748025

    Gene expression analysis of Mel501 cells cultured in buffered (Mel501) and in acidic conditions (Mel501ac). Transcripts were determined in a StepOne RT-PCR machine (Applied Biosystems, USA) using SYBR ® Select Master Mix (Life Technologies). Panel (A) displays the fold change in Mel501ac with respect to Mel501. GAPDH gene was used as endogenous control (set 1). Panel (B) reports the fold change of SCD5 and ELOVL7 gene in Mel501 cultivated at progressively lower pH. For each experiment, the analysis was repeated three times in triplicate. The mean ± SD of a representative experiment is reported (* p
    Figure Legend Snippet: Gene expression analysis of Mel501 cells cultured in buffered (Mel501) and in acidic conditions (Mel501ac). Transcripts were determined in a StepOne RT-PCR machine (Applied Biosystems, USA) using SYBR ® Select Master Mix (Life Technologies). Panel (A) displays the fold change in Mel501ac with respect to Mel501. GAPDH gene was used as endogenous control (set 1). Panel (B) reports the fold change of SCD5 and ELOVL7 gene in Mel501 cultivated at progressively lower pH. For each experiment, the analysis was repeated three times in triplicate. The mean ± SD of a representative experiment is reported (* p

    Techniques Used: Expressing, Cell Culture, Step One RT-PCR

    5) Product Images from "Disruption of the Zdhhc9 intellectual disability gene leads to behavioural abnormalities in a mouse model"

    Article Title: Disruption of the Zdhhc9 intellectual disability gene leads to behavioural abnormalities in a mouse model

    Journal: Experimental Neurology

    doi: 10.1016/j.expneurol.2018.06.014

    PCR and immunoblotting analysis of Zdhhc9 expression in wild-type and mutant mice. (A) Agarose gel electrophoresis of end-point PCR products from WT and mutant brain cDNA samples. mRNA confirmation assay 1 (MA1) primers were designed to amplify a Zdhhc9 mRNA region of 283 bp from the 5′UTR to Exon 2. mRNA confirmation assay 2 (MA2) primers were designed to amplify a Zdhhc9 mRNA region of 154 bp from Exon 2 to Exon 3. z9v1 primers were designed to amplify a mRNA region of 139 bp from exon 5 to exon 7, and z9v2 primers were designed to amplify a mRNA region of 106 bp from exon 5 to exon 7. HyperLadder 100 bp ladder (Bioline) was used as a marker of DNA size. (B) Comparison of the average ΔCt values of WT and mutant mouse brain samples ( n = 3 WT, 3 mutant) for MA2 and z9v1 after normalisation against Tbp and Hprt1 reference genes. cDNA from WT and mutant mouse brain was amplified for 40 cycles using specific primers for the different targets (MA2, z9v1, Tbp and Hprt1 ) and SYBR Select Master Mix. Statistical analysis (unpaired t -test, Minitab version 17) revealed a significant effect of genotype for MA2- Tbp ( p = .042), MA2- Hprt1 ( p = .046) and z9v1- Hprt1 ( p = .037). p value for z9v1- Tbp was 0.075. (C) PCR products amplified from cDNA derived from mRNA extracted from WT and Zdhhc9 mutant mouse brain. Primers were designed to anneal to a region in exon 1 (see MAO F in Table 1 for sequence of primer) and the 3′-UTR (3UTR R in Table 1 for sequence of primer). HyperLadder 1 kb ladder (Bioline) was included as marker of DNA size. (D) Lysates from HEK293T cells transfected with HA-tagged zDHHCs were probed with antibodies against zDHHC9 ( top ) and HA ( bottom ). (E) Brain lysates from WT and mutant mice were probed with antibodies against zDHHC9 ( top ) and beta actin ( bottom ). Position of molecular weight markers is shown on the left of all immunoblots.
    Figure Legend Snippet: PCR and immunoblotting analysis of Zdhhc9 expression in wild-type and mutant mice. (A) Agarose gel electrophoresis of end-point PCR products from WT and mutant brain cDNA samples. mRNA confirmation assay 1 (MA1) primers were designed to amplify a Zdhhc9 mRNA region of 283 bp from the 5′UTR to Exon 2. mRNA confirmation assay 2 (MA2) primers were designed to amplify a Zdhhc9 mRNA region of 154 bp from Exon 2 to Exon 3. z9v1 primers were designed to amplify a mRNA region of 139 bp from exon 5 to exon 7, and z9v2 primers were designed to amplify a mRNA region of 106 bp from exon 5 to exon 7. HyperLadder 100 bp ladder (Bioline) was used as a marker of DNA size. (B) Comparison of the average ΔCt values of WT and mutant mouse brain samples ( n = 3 WT, 3 mutant) for MA2 and z9v1 after normalisation against Tbp and Hprt1 reference genes. cDNA from WT and mutant mouse brain was amplified for 40 cycles using specific primers for the different targets (MA2, z9v1, Tbp and Hprt1 ) and SYBR Select Master Mix. Statistical analysis (unpaired t -test, Minitab version 17) revealed a significant effect of genotype for MA2- Tbp ( p = .042), MA2- Hprt1 ( p = .046) and z9v1- Hprt1 ( p = .037). p value for z9v1- Tbp was 0.075. (C) PCR products amplified from cDNA derived from mRNA extracted from WT and Zdhhc9 mutant mouse brain. Primers were designed to anneal to a region in exon 1 (see MAO F in Table 1 for sequence of primer) and the 3′-UTR (3UTR R in Table 1 for sequence of primer). HyperLadder 1 kb ladder (Bioline) was included as marker of DNA size. (D) Lysates from HEK293T cells transfected with HA-tagged zDHHCs were probed with antibodies against zDHHC9 ( top ) and HA ( bottom ). (E) Brain lysates from WT and mutant mice were probed with antibodies against zDHHC9 ( top ) and beta actin ( bottom ). Position of molecular weight markers is shown on the left of all immunoblots.

    Techniques Used: Polymerase Chain Reaction, Expressing, Mutagenesis, Mouse Assay, Agarose Gel Electrophoresis, Marker, Amplification, Derivative Assay, Sequencing, Transfection, Molecular Weight, Western Blot

    6) Product Images from "The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection"

    Article Title: The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection

    Journal: mBio

    doi: 10.1128/mBio.02567-18

    IFNs decrease Vps4A and Vps4B mRNA levels in TG mouse neurons. (A) mRNA levels of Vps4A, Vps4B, Chmp4B, Chmp2B, and Isg15 were analyzed by qPCR using TG mRNA from infected LC3-GFP +/− mice during the indicated times. Quantification of Sybr Green signal was normalized to β-actin; n = 5 for all genes analyzed and all time points, except for Chmp2B Mock ( n = 3). All samples were performed with two technical replicates. *, P
    Figure Legend Snippet: IFNs decrease Vps4A and Vps4B mRNA levels in TG mouse neurons. (A) mRNA levels of Vps4A, Vps4B, Chmp4B, Chmp2B, and Isg15 were analyzed by qPCR using TG mRNA from infected LC3-GFP +/− mice during the indicated times. Quantification of Sybr Green signal was normalized to β-actin; n = 5 for all genes analyzed and all time points, except for Chmp2B Mock ( n = 3). All samples were performed with two technical replicates. *, P

    Techniques Used: Real-time Polymerase Chain Reaction, Infection, Mouse Assay, SYBR Green Assay

    7) Product Images from "The Influence of E1A C-Terminus on Adenovirus Replicative Cycle"

    Article Title: The Influence of E1A C-Terminus on Adenovirus Replicative Cycle

    Journal: Viruses

    doi: 10.3390/v9120387

    C-terminus deletions in E1A affect induction of cell cycle genes in arrested cells. Arrested WI-38 cells were infected with the indicated mutant viruses at a MOI of 100. At the indicated time points, total RNA was extracted using the TRIzol reagent and treated with DNase I. RNA was converted to cDNA using VILO Master Mix reverse transcriptase and relative levels of the indicated cellular mRNAs were quantified by qPCR using BioRad CFX96 with Applied Biosystems SYBR Master Mix for CFX. Expression levels were compared to those obtained from mock-infected cells using the Pfaffl method with GAPDH as reference. Statistically significant changes as compared to genes expressed in dl 309-infected cells are indicated with an asterix (*), others are not significant. For statistically significant differences, the p values are as follows: BLM — p ≤ 0.0001; PCNA — p ≤ 0.0132; MCM4 — p ≤ 0.0003. Error bars represent standard deviation of biological replicates, n = 3.
    Figure Legend Snippet: C-terminus deletions in E1A affect induction of cell cycle genes in arrested cells. Arrested WI-38 cells were infected with the indicated mutant viruses at a MOI of 100. At the indicated time points, total RNA was extracted using the TRIzol reagent and treated with DNase I. RNA was converted to cDNA using VILO Master Mix reverse transcriptase and relative levels of the indicated cellular mRNAs were quantified by qPCR using BioRad CFX96 with Applied Biosystems SYBR Master Mix for CFX. Expression levels were compared to those obtained from mock-infected cells using the Pfaffl method with GAPDH as reference. Statistically significant changes as compared to genes expressed in dl 309-infected cells are indicated with an asterix (*), others are not significant. For statistically significant differences, the p values are as follows: BLM — p ≤ 0.0001; PCNA — p ≤ 0.0132; MCM4 — p ≤ 0.0003. Error bars represent standard deviation of biological replicates, n = 3.

    Techniques Used: Infection, Mutagenesis, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation

    Relative viral gene expression profiles as compared to dl 309. Arrested WI-38 cells were infected at a MOI of 100 and total RNA was extracted at the indicated time points using the TRIzol reagent and treated with DNase I. RNA was converted to cDNA using VILO Master Mix reverse transcriptase and relative levels of viral mRNAs were quantified by qPCR using BioRad CFX96 with Applied Biosystems SYBR Master Mix for CFX. Expression levels were compared to those obtained from dl 309-infected cells using the Pfaffl method with GAPDH as a reference. Statistically insignificant differences are indicated with an n . The p values for all the genes at a time point that are significant are: 16 h— p
    Figure Legend Snippet: Relative viral gene expression profiles as compared to dl 309. Arrested WI-38 cells were infected at a MOI of 100 and total RNA was extracted at the indicated time points using the TRIzol reagent and treated with DNase I. RNA was converted to cDNA using VILO Master Mix reverse transcriptase and relative levels of viral mRNAs were quantified by qPCR using BioRad CFX96 with Applied Biosystems SYBR Master Mix for CFX. Expression levels were compared to those obtained from dl 309-infected cells using the Pfaffl method with GAPDH as a reference. Statistically insignificant differences are indicated with an n . The p values for all the genes at a time point that are significant are: 16 h— p

    Techniques Used: Expressing, Infection, Real-time Polymerase Chain Reaction

    8) Product Images from "The source of SYBR green master mix determines outcome of nucleic acid amplification reactions"

    Article Title: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions

    Journal: BMC Research Notes

    doi: 10.1186/s13104-016-2093-4

    Not all PCR mixes result in optimal specificity of cDNA amplification reactions. The performance of three commercial SYBR green PCR mixes was compared by amplifying cDNA with 70 primer-based assays targeting different mRNA transcripts. A single melting curve peak indicates specificity of the amplification. The figure shows examples of melting curves and corresponding gel blots for several primer sets from Table 3 , in situations where all three mixes gave optimal results and where one or more mixes resulted in a suboptimal amplification reaction
    Figure Legend Snippet: Not all PCR mixes result in optimal specificity of cDNA amplification reactions. The performance of three commercial SYBR green PCR mixes was compared by amplifying cDNA with 70 primer-based assays targeting different mRNA transcripts. A single melting curve peak indicates specificity of the amplification. The figure shows examples of melting curves and corresponding gel blots for several primer sets from Table 3 , in situations where all three mixes gave optimal results and where one or more mixes resulted in a suboptimal amplification reaction

    Techniques Used: Polymerase Chain Reaction, Amplification, SYBR Green Assay

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Disruption of the Zdhhc9 intellectual disability gene leads to behavioural abnormalities in a mouse model
    Article Snippet: .. Quantitative PCR (RT-qPCR) was performed in an Applied Biosystems StepOnePlus Real-Time PCR System, using the SYBR Select Master Mix (Life Technologies). .. The PCR reaction consisted of an initial polymerase activation step of 2 min at 95 °C, followed by 40 cycles of a denaturing stage of 15 s at 95 °C and an annealing and extension step at 60 °C for 1 min. A post-PCR melt curve analysis was included for qualitative analyses of the PCR product.

    Article Title: Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1)
    Article Snippet: .. Quantitative real time PCR (qRT-PCR) was performed on a ViiA7 with SYBR Select Master Mix (Life Technologies). .. Relative mRNA expression was calculated by the comparative ΔΔCt-Method normalized to the housekeeping gene ribosomal protein, large, P0 (RPLP0).

    Article Title: The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection
    Article Snippet: .. SYBR Select master mix (Life Technologies) was used, and RT-qPCR was performed using a CFX96 Touch real-time PCR detection system. .. Oligonucleotides were as follows: β-actin, Fw, AGT GTG ACG TTG ACA TCC GT, and Rv, TGC TAG GAG CCA GAG CAG TA; Isg15, Fw, TGA GCA TCC TGG TGA GGA ACG AAA, and Rv, AGC CAG AAC TGG TCT TCG TGA CTT; Vps4A, Fw, GAC AAC GTC AAC CCT CCA GA, and Rv, AGC ATG CTG GTA GAG ACG GA; Vps4B, Fw, GCC TTG TCT GTA GTA GGG GAC, and Rv, TTC CCA GCT TTG TCT TCC TGG; Chmp4B, Fw, GCC CGA AAC AGT CCC TCT AC, and Rv, TTC CTT CTT CTT GGC GGG TT; Chmp2B, Fw, AAG CAG CTT GTC CAC CTA CG, and Rv, TTG CAT TGT CTT TGC AGT GGT.

    Article Title: Rotavirus Nonstructural Protein 1 Suppresses Virus-Induced Cellular Apoptosis To Facilitate Viral Growth by Activating the Cell Survival Pathways during Early Stages of Infection ▿Rotavirus Nonstructural Protein 1 Suppresses Virus-Induced Cellular Apoptosis To Facilitate Viral Growth by Activating the Cell Survival Pathways during Early Stages of Infection ▿ ‡
    Article Snippet: .. RNA was extracted with Trizol reagent (Invitrogen), and a quantitative PCR was performed in triplicates with SYBR green Mastermix using an ABI7500 (Applied Biosystems, Inc., Foster City, CA). .. Specific primers for VP6 (VP6-F-5′-GCACAGCCATTCGAACATCATGC-3′ and VP6-R5′-TGCATCGGCGAGTACAGAC TAC-3′), GAPDH (F-5′-GAGAACGGGAAGCTTGTCATC-3′ and R-5′-CATGACGAACATGGGGGCATC-3′), ISG56 (F-5′-TGGGCCTTGCTGAAGT-3′ and R-5′-GGCCCATCCTTCCTCA-3′), IFN-β TaqMan probe ID Hs01077958_s1, and IRF3 TaqMan probe ID Hs00155574_m1 were used.

    Step One RT-PCR:

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. 4.8. qRT-PCR RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Disruption of the Zdhhc9 intellectual disability gene leads to behavioural abnormalities in a mouse model
    Article Snippet: .. Quantitative PCR (RT-qPCR) was performed in an Applied Biosystems StepOnePlus Real-Time PCR System, using the SYBR Select Master Mix (Life Technologies). .. The PCR reaction consisted of an initial polymerase activation step of 2 min at 95 °C, followed by 40 cycles of a denaturing stage of 15 s at 95 °C and an annealing and extension step at 60 °C for 1 min. A post-PCR melt curve analysis was included for qualitative analyses of the PCR product.

    Article Title: Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1)
    Article Snippet: .. Quantitative real time PCR (qRT-PCR) was performed on a ViiA7 with SYBR Select Master Mix (Life Technologies). .. Relative mRNA expression was calculated by the comparative ΔΔCt-Method normalized to the housekeeping gene ribosomal protein, large, P0 (RPLP0).

    Article Title: The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection
    Article Snippet: .. SYBR Select master mix (Life Technologies) was used, and RT-qPCR was performed using a CFX96 Touch real-time PCR detection system. .. Oligonucleotides were as follows: β-actin, Fw, AGT GTG ACG TTG ACA TCC GT, and Rv, TGC TAG GAG CCA GAG CAG TA; Isg15, Fw, TGA GCA TCC TGG TGA GGA ACG AAA, and Rv, AGC CAG AAC TGG TCT TCG TGA CTT; Vps4A, Fw, GAC AAC GTC AAC CCT CCA GA, and Rv, AGC ATG CTG GTA GAG ACG GA; Vps4B, Fw, GCC TTG TCT GTA GTA GGG GAC, and Rv, TTC CCA GCT TTG TCT TCC TGG; Chmp4B, Fw, GCC CGA AAC AGT CCC TCT AC, and Rv, TTC CTT CTT CTT GGC GGG TT; Chmp2B, Fw, AAG CAG CTT GTC CAC CTA CG, and Rv, TTG CAT TGT CTT TGC AGT GGT.

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. 4.8. qRT-PCR RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    SYBR Green Assay:

    Article Title: A Transcriptionally Permissive Epigenetic Landscape at the Vasoactive Intestinal Peptide Receptor-1 Promoter Suggests an Euchromatin Nuclear Position in Murine CD4 T Cells
    Article Snippet: .. SYBR green master mix and Taqman master mix were obtained from Applied Biosystems. .. The following antibodies (and clones) were obtained from Biolegend: anti-CD4-FITC (RM4-4), anti-CD4-FITC-PE-Cy5 (G1C1.5), anti-CD25-PE (3C7), anti-CD43-PE (1B1), anti-CD44-PE (IM7), anti-CD127-PE (1L-7Ra), anti-CD62L-PE (Mel-14), anti-CD117-PE (ACK2), anti-CD44-FITC (IM7), antiCD-69-PE (H1,2F3) and antiCD45R-FITC (RA3-6B2).

    Article Title: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
    Article Snippet: .. Performance of three different PCR mixes was compared, including SYBR Select Master Mix (Applied Biosystems), iQ SYBR green supermix (Bio-Rad, Hercules, CA, USA), and FastStart essential DNA Green Master (Roche Diagnostics). .. We evaluated four different HRM mixes on the lighter cycler 96, namely high resolution melting master (Roche Diagnostics), SensiFast HRM Kit (Bioline, London, UK), qPCRBIO HRM Mix(PCR Biosystems, London, UK), and MeltDoctor HRM Master Mix (Applied Biosystems).

    Article Title: Rotavirus Nonstructural Protein 1 Suppresses Virus-Induced Cellular Apoptosis To Facilitate Viral Growth by Activating the Cell Survival Pathways during Early Stages of Infection ▿Rotavirus Nonstructural Protein 1 Suppresses Virus-Induced Cellular Apoptosis To Facilitate Viral Growth by Activating the Cell Survival Pathways during Early Stages of Infection ▿ ‡
    Article Snippet: .. RNA was extracted with Trizol reagent (Invitrogen), and a quantitative PCR was performed in triplicates with SYBR green Mastermix using an ABI7500 (Applied Biosystems, Inc., Foster City, CA). .. Specific primers for VP6 (VP6-F-5′-GCACAGCCATTCGAACATCATGC-3′ and VP6-R5′-TGCATCGGCGAGTACAGAC TAC-3′), GAPDH (F-5′-GAGAACGGGAAGCTTGTCATC-3′ and R-5′-CATGACGAACATGGGGGCATC-3′), ISG56 (F-5′-TGGGCCTTGCTGAAGT-3′ and R-5′-GGCCCATCCTTCCTCA-3′), IFN-β TaqMan probe ID Hs01077958_s1, and IRF3 TaqMan probe ID Hs00155574_m1 were used.

    Expressing:

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. 4.8. qRT-PCR RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
    Article Snippet: .. Performance of three different PCR mixes was compared, including SYBR Select Master Mix (Applied Biosystems), iQ SYBR green supermix (Bio-Rad, Hercules, CA, USA), and FastStart essential DNA Green Master (Roche Diagnostics). .. We evaluated four different HRM mixes on the lighter cycler 96, namely high resolution melting master (Roche Diagnostics), SensiFast HRM Kit (Bioline, London, UK), qPCRBIO HRM Mix(PCR Biosystems, London, UK), and MeltDoctor HRM Master Mix (Applied Biosystems).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher powerup sybr green qpcr kit
    Accumulation of immunostimulatory 5′-ppp-vtRNAs during lytic reactivation. a Predicted secondary structure of vtRNAs generated by RNAfold. b <t>SYBR-Gold</t> staining of in vitro transcribed vtRNAs with or without CIP treatment. c HCT116 ISG54-luciferase reporter cells were transfected with 100 ng in vitro transcribed vtRNAs with or without CIP treatment. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. d BC-3 cells were reactivated for 3 days and expression of DUSP11 was quantified by <t>RT-qPCR.</t> L latency, D1–D3 lytic reactivation for 1 day to 3 days. The DUSP11 expression was normalized to the level of 18S rRNA and L was set as 1. e Cell lysates were prepared from BC-3 cells described in ( d ) and DUSP11 protein levels were monitored by Western blot. GAPDH was run as a loading control. f Latent and lytic BC-3 cells were subjected to RNAP II ChIP-qPCR analysis. Signals were normalized to input. g ). h HCT116 ISG54-luciferase reporter cells were transfected with vtRNA or U1 RNA isolated by antisense oligonucleotide affinity selection from either latent or lytic BC-3 cells. Cells were harvested 12 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, * p
    Powerup Sybr Green Qpcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powerup sybr green qpcr kit/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    powerup sybr green qpcr kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybr select master mix
    Accumulation of immunostimulatory 5′-ppp-vtRNAs during lytic reactivation. a Predicted secondary structure of vtRNAs generated by RNAfold. b <t>SYBR-Gold</t> staining of in vitro transcribed vtRNAs with or without CIP treatment. c HCT116 ISG54-luciferase reporter cells were transfected with 100 ng in vitro transcribed vtRNAs with or without CIP treatment. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. d BC-3 cells were reactivated for 3 days and expression of DUSP11 was quantified by <t>RT-qPCR.</t> L latency, D1–D3 lytic reactivation for 1 day to 3 days. The DUSP11 expression was normalized to the level of 18S rRNA and L was set as 1. e Cell lysates were prepared from BC-3 cells described in ( d ) and DUSP11 protein levels were monitored by Western blot. GAPDH was run as a loading control. f Latent and lytic BC-3 cells were subjected to RNAP II ChIP-qPCR analysis. Signals were normalized to input. g ). h HCT116 ISG54-luciferase reporter cells were transfected with vtRNA or U1 RNA isolated by antisense oligonucleotide affinity selection from either latent or lytic BC-3 cells. Cells were harvested 12 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, * p
    Sybr Select Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr select master mix/product/Thermo Fisher
    Average 99 stars, based on 681 article reviews
    Price from $9.99 to $1999.99
    sybr select master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Accumulation of immunostimulatory 5′-ppp-vtRNAs during lytic reactivation. a Predicted secondary structure of vtRNAs generated by RNAfold. b SYBR-Gold staining of in vitro transcribed vtRNAs with or without CIP treatment. c HCT116 ISG54-luciferase reporter cells were transfected with 100 ng in vitro transcribed vtRNAs with or without CIP treatment. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. d BC-3 cells were reactivated for 3 days and expression of DUSP11 was quantified by RT-qPCR. L latency, D1–D3 lytic reactivation for 1 day to 3 days. The DUSP11 expression was normalized to the level of 18S rRNA and L was set as 1. e Cell lysates were prepared from BC-3 cells described in ( d ) and DUSP11 protein levels were monitored by Western blot. GAPDH was run as a loading control. f Latent and lytic BC-3 cells were subjected to RNAP II ChIP-qPCR analysis. Signals were normalized to input. g ). h HCT116 ISG54-luciferase reporter cells were transfected with vtRNA or U1 RNA isolated by antisense oligonucleotide affinity selection from either latent or lytic BC-3 cells. Cells were harvested 12 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, * p

    Journal: Nature Communications

    Article Title: RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection

    doi: 10.1038/s41467-018-07314-7

    Figure Lengend Snippet: Accumulation of immunostimulatory 5′-ppp-vtRNAs during lytic reactivation. a Predicted secondary structure of vtRNAs generated by RNAfold. b SYBR-Gold staining of in vitro transcribed vtRNAs with or without CIP treatment. c HCT116 ISG54-luciferase reporter cells were transfected with 100 ng in vitro transcribed vtRNAs with or without CIP treatment. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. d BC-3 cells were reactivated for 3 days and expression of DUSP11 was quantified by RT-qPCR. L latency, D1–D3 lytic reactivation for 1 day to 3 days. The DUSP11 expression was normalized to the level of 18S rRNA and L was set as 1. e Cell lysates were prepared from BC-3 cells described in ( d ) and DUSP11 protein levels were monitored by Western blot. GAPDH was run as a loading control. f Latent and lytic BC-3 cells were subjected to RNAP II ChIP-qPCR analysis. Signals were normalized to input. g ). h HCT116 ISG54-luciferase reporter cells were transfected with vtRNA or U1 RNA isolated by antisense oligonucleotide affinity selection from either latent or lytic BC-3 cells. Cells were harvested 12 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, * p

    Article Snippet: RNA was DNase I (NEB) treated at 37 °C for 20 min, and inactivated with EDTA at 70 °C for 10 min. cDNA was synthesized from DNase-treated RNA with random 9-mer (Integrated DNA Technologies) and M-MLV RT (Promega). qPCR was performed using the PowerUp SYBR Green qPCR kit (Thermo Scientific) with appropriate primers (Supplementary Table ).

    Techniques: Generated, Staining, In Vitro, Luciferase, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Isolation, Selection