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TaKaRa sybr premix ex taq
Sybr Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RNA Extraction:

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland).

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: Paragraph title: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols.

Amplification:

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA). .. All PCRs were done in triplicate on a 96-well RT-PCR plate under the following conditions (two-step amplification): 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s, and 60 °C for 34 s. A subsequent melting curve (95 °C for 15 s, 60 °C for 1 min and 95 °C for 15 s) with continuous fluorescence measurement and final cooling to 25 °C was processed.

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. The amplification efficiency of the target gene and housekeeping gene were estimated by using E = (10−1/slope )−1, where the slope was derived from the plot of the cycle threshold (Ct) value versus the log of the serially diluted template concentration.

Article Title: Autophagy inhibits chemotherapy-induced apoptosis through downregulating Bad and Bim in hepatocellular carcinoma cells
Article Snippet: The mRNA expression of the genes was analyzed with specific primers as follows: 5′-GAAGGTGAAGGTCGGAGTC-3′, 5′-GAAGATGGTGATGGGATTTC-3′ for GAPDH, and the length of this amplicon was 225 bp; 5′-CGGAGGATGAGTGACGAGTT-3′, 5′-GATGTGGAGCGAAGGTCACT-3′ for Bad, and the length of this amplicon was 180 bp; 5′-TAAGTTCTGAGTGTGACCGAGA-3′, 5′-GCTCTGTCTGTAGGGAGGTAGG-3′ for Bim, and the length of this amplicon was 96 bp. .. The q-PCR was conducted in a Roche LightCycler480 using the SYBR Premix Ex Taq (TaKaRa, Cat. DRR420A).

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: .. The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols. .. The primer sequences for SCIN gene amplification were as follows: Forward, AGGAAGGTCTGAACTAAT and reverse, CTGTTACTTATGTCTGCTAT.

Agarose Gel Electrophoresis:

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: Meanwhile, the integrity of RNA in each sample was assessed using 1% denatured agarose gel electrophoresis. .. The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA).

Fluorescence:

Article Title: Let-7a inhibits proliferation and promotes apoptosis of human asthmatic airway smooth muscle cells
Article Snippet: .. This reaction was performed at 25°C for 5 min, 50°C for 20 min then 75°C for 5 min. qPCR was performed with SYBR® Premix Ex Taq™ (Takara Bio Inc., Otsu, Japan; cat. no. DRR041A) according to the manufacturer's instructions, using an ABI 7500 real-time fluorescence qPCR machine (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA). .. All PCRs were done in triplicate on a 96-well RT-PCR plate under the following conditions (two-step amplification): 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s, and 60 °C for 34 s. A subsequent melting curve (95 °C for 15 s, 60 °C for 1 min and 95 °C for 15 s) with continuous fluorescence measurement and final cooling to 25 °C was processed.

Synthesized:

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: RNA extraction and RT-qPCR Total RNA from the frozen tissue specimens was extracted using TRIzol reagent (cat. no. 9108), and cDNA was synthesized from 0.5 µg of RNA using RT reagents (cat. no. DRR036s; both Takara, Dalian, China). qPCR was performed according to the manufacturer's protocol. .. SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland).

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: Total RNA was reverse-transcribed using the Prime-Script® RT reagent kit with gDNA Eraser (Cat. RR047A, Takara, Dalian, China) according to the manufacturer’s instructions. cDNA was synthesized and stored at −20 °C for further analysis. .. The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA).

Article Title: De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes
Article Snippet: Complementary DNA was synthesized using an equal amount of total RNA (1 μg) from the two samples according to the instructions of the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China). .. Each 20 μL of PCR master mix contained 2 μL of diluted cDNA template, 10 μL of SYBR Premix Ex Taq™, 0.4 μL of forward primer (10 μM), 0.4 μL of reverse primer (10 μM), 0.4 μL of Rox Reference Dye II (50×) and 6.8 μL of RNase-free H2 O, according to the instructions of SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China).

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: Complementary DNA was synthesized with equal amount of total RNA (1 ug) from eggs, first-instar larvae, third-instar larvae, fifth-instar larvae, pupae and adults according to the instructions of the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China). .. Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China).

Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis
Article Snippet: Total RNA (500 ng/µl aliquots) were reverse-transcribed into cDNA using a PrimeScript RT Reagent kit (cat. no. DRR037S; Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using the SYBR Premix Ex Taq™ (cat. no. DRR041S; Takara Biotechnology Co., Ltd.). .. The following gene-specific primers were designed and synthesized by Takara Biotechnology Co., Ltd. and used for the qPCR: Col-I forward, 5′-CTCCTGGCAAGAACGGAGATG-3′ and reverse, 5′-CTGTTCCAGGCAATCCACGA-3′; β-actin forward, 5′-CCCGCGAGTACAACCTTCTT-3′ and reverse, 5′-TCATCCATGGCGAACTGGTG-3′.

Multiple Displacement Amplification:

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: Total RNA was extracted from BC MDA-MB-231, T-47D and MCF-7 cell lines respectively using TRIzol reagent (Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols.

Isolation:

Article Title: Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer
Article Snippet: Total RNA was isolated from cells and tissue samples using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. RNA was reverse transcribed using the PrimeScript RT reagent kit (cat. no. RR037; Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol, and then processed for qPCR using SYBR Premix Ex Taq (cat. no. RR390; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol.

Article Title: IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production
Article Snippet: Paragraph title: Isolation of RNA, q-PCR, and semi-quantitative RT-PCR ... Then, q-PCR was performed using a 96-well CFX-96 detection system (Bio-Rad Laboratories) with SYBR Premix Ex Taq™ (Takara, Cat. No. DRR041A).

Article Title: SNX10 promotes phagosome maturation in macrophages and protects mice against Listeria monocytogenes infection
Article Snippet: Paragraph title: RNA isolation and quantitative PCR (Q-PCR) ... Then, Q-PCR was performed using a 96-well CFX-96 detection system (Bio-Rad Laboratories) with SYBR Premix Ex Taq™ (TaKaRa, Cat. No. DRR041A).

Article Title: Autophagy inhibits chemotherapy-induced apoptosis through downregulating Bad and Bim in hepatocellular carcinoma cells
Article Snippet: Paragraph title: RNA isolation, reverse transcription, and quantitative real-time PCR (q-PCR) ... The q-PCR was conducted in a Roche LightCycler480 using the SYBR Premix Ex Taq (TaKaRa, Cat. DRR420A).

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: The cDNA was retro-transcribed from isolated RNA using the M-MLV kit (cat no. M1705, Promega Corporation, Madison, WI, USA). .. The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols.

Plasmid Preparation:

Article Title: Lime and ammonium carbonate fumigation coupled with bio‐organic fertilizer application steered banana rhizosphere to assemble a unique microbiome against Panama disease
Article Snippet: Standard curves were constructed using 10‐fold serial dilutions of a plasmid containing a copy of the ITS fragment from Foc 4. .. Quantitative PCR amplifications were performed in a 20‐μl mixture for each reaction containing 2 μl template DNA, 10 μl SYBR® Premix Ex Taq™ (TaKaRa, Shiga, Ja pan), 0.4 μl of each primer, 0.4 μl ROX Reference Dye II and nuclease‐free water.

Size-exclusion Chromatography:

Article Title: Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer
Article Snippet: RNA was reverse transcribed using the PrimeScript RT reagent kit (cat. no. RR037; Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol, and then processed for qPCR using SYBR Premix Ex Taq (cat. no. RR390; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. Briefly, the qPCR reaction mixture was preheated to 95°C for 10 sec, followed by 35 cycles consisting of 95°C for 15 sec, 65°C for 30 sec and 72°C for 30 sec. GAPDH was used as an internal control.

Article Title: Let-7a inhibits proliferation and promotes apoptosis of human asthmatic airway smooth muscle cells
Article Snippet: This reaction was performed at 25°C for 5 min, 50°C for 20 min then 75°C for 5 min. qPCR was performed with SYBR® Premix Ex Taq™ (Takara Bio Inc., Otsu, Japan; cat. no. DRR041A) according to the manufacturer's instructions, using an ABI 7500 real-time fluorescence qPCR machine (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. The reaction conditions for PCR were as follows: Initial denaturation at 95°C for 3 min and 40 cycles of 95°C for 15 sec and 60°C for 30 sec.

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland). .. The PCR conditions were as follows: Initial denaturation at 95°C for 30 sec; followed by 35 cycles of denaturation at 94°C for 30 sec, annealing at 50°C for 30 sec, and extension at 70°C for 30 sec.

Article Title: miRNA-21 enhances chemoresistance to cisplatin in epithelial ovarian cancer by negatively regulating PTEN
Article Snippet: RT was performed using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) at 16°C for 30 min, 42°C for 30 min and 85°C for 5 min. qPCR was performed using SYBR Premix Ex Taq (Takara Bio, Inc.) according to the manufacturer's protocol, with 1.33 µl cDNA from the RT reaction. .. The thermocycler conditions were, an initial 15 min at 95°C, then 40 cycles of 15 sec at 95°C and 60 sec at 60°C.

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: .. The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols. .. The primer sequences for SCIN gene amplification were as follows: Forward, AGGAAGGTCTGAACTAAT and reverse, CTGTTACTTATGTCTGCTAT.

Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis
Article Snippet: Total RNA (500 ng/µl aliquots) were reverse-transcribed into cDNA using a PrimeScript RT Reagent kit (cat. no. DRR037S; Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using the SYBR Premix Ex Taq™ (cat. no. DRR041S; Takara Biotechnology Co., Ltd.). .. The following thermocycling conditions were used for the qPCR: Initial denaturation at 95°C for 10 sec, 40 cycles of 95°C for 5 sec and 60°C for 20 sec using the LightCycle 2.0 real-time PCR System (Roche Diagnostics, Indianapolis, IN, USA).

Quantitative RT-PCR:

Article Title: Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... RNA was reverse transcribed using the PrimeScript RT reagent kit (cat. no. RR037; Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol, and then processed for qPCR using SYBR Premix Ex Taq (cat. no. RR390; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol.

Article Title: CDK11p58 inhibits ERα-positive breast cancer invasion by targeting integrin β3 via the repression of ERα signaling
Article Snippet: Paragraph title: TaqMan® Array human metastasis gene expression assays and quantitative reverse transcription polymerase chain reaction (qRT-PCR) ... After converting total RNA to cDNA in a reverse transcription (RT) reaction by Cdna Synthesis Kit (Cat. 6110A, TaKaRa, Dalian, China), qPCR were used to quantitate the mRNA expression levels by SYBR ® Premix Ex Taq™(Cat. RR420Q, TaKaRa).

Article Title: Let-7a inhibits proliferation and promotes apoptosis of human asthmatic airway smooth muscle cells
Article Snippet: Paragraph title: RT-qPCR ... This reaction was performed at 25°C for 5 min, 50°C for 20 min then 75°C for 5 min. qPCR was performed with SYBR® Premix Ex Taq™ (Takara Bio Inc., Otsu, Japan; cat. no. DRR041A) according to the manufacturer's instructions, using an ABI 7500 real-time fluorescence qPCR machine (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland).

Article Title: miRNA-21 enhances chemoresistance to cisplatin in epithelial ovarian cancer by negatively regulating PTEN
Article Snippet: Paragraph title: RT-qPCR analysis ... RT was performed using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) at 16°C for 30 min, 42°C for 30 min and 85°C for 5 min. qPCR was performed using SYBR Premix Ex Taq (Takara Bio, Inc.) according to the manufacturer's protocol, with 1.33 µl cDNA from the RT reaction.

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: RNA was used for quantitative RT-PCR analysis when the sample had a 28S/18S rRNA ratio ≥ 1.8 [ ]. .. The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA).

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: Paragraph title: qRT-PCR analysis of Of RyR expression ... Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China).

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: .. The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols. .. The primer sequences for SCIN gene amplification were as follows: Forward, AGGAAGGTCTGAACTAAT and reverse, CTGTTACTTATGTCTGCTAT.

Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... Total RNA (500 ng/µl aliquots) were reverse-transcribed into cDNA using a PrimeScript RT Reagent kit (cat. no. DRR037S; Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using the SYBR Premix Ex Taq™ (cat. no. DRR041S; Takara Biotechnology Co., Ltd.).

Real-time Polymerase Chain Reaction:

Article Title: Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer
Article Snippet: .. RNA was reverse transcribed using the PrimeScript RT reagent kit (cat. no. RR037; Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol, and then processed for qPCR using SYBR Premix Ex Taq (cat. no. RR390; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. Briefly, the qPCR reaction mixture was preheated to 95°C for 10 sec, followed by 35 cycles consisting of 95°C for 15 sec, 65°C for 30 sec and 72°C for 30 sec. GAPDH was used as an internal control.

Article Title: CDK11p58 inhibits ERα-positive breast cancer invasion by targeting integrin β3 via the repression of ERα signaling
Article Snippet: .. After converting total RNA to cDNA in a reverse transcription (RT) reaction by Cdna Synthesis Kit (Cat. 6110A, TaKaRa, Dalian, China), qPCR were used to quantitate the mRNA expression levels by SYBR ® Premix Ex Taq™(Cat. RR420Q, TaKaRa). ..

Article Title: Lime and ammonium carbonate fumigation coupled with bio‐organic fertilizer application steered banana rhizosphere to assemble a unique microbiome against Panama disease
Article Snippet: .. Quantitative PCR amplifications were performed in a 20‐μl mixture for each reaction containing 2 μl template DNA, 10 μl SYBR® Premix Ex Taq™ (TaKaRa, Shiga, Ja pan), 0.4 μl of each primer, 0.4 μl ROX Reference Dye II and nuclease‐free water. ..

Article Title: Let-7a inhibits proliferation and promotes apoptosis of human asthmatic airway smooth muscle cells
Article Snippet: .. This reaction was performed at 25°C for 5 min, 50°C for 20 min then 75°C for 5 min. qPCR was performed with SYBR® Premix Ex Taq™ (Takara Bio Inc., Otsu, Japan; cat. no. DRR041A) according to the manufacturer's instructions, using an ABI 7500 real-time fluorescence qPCR machine (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: .. SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland). .. The PCR conditions were as follows: Initial denaturation at 95°C for 30 sec; followed by 35 cycles of denaturation at 94°C for 30 sec, annealing at 50°C for 30 sec, and extension at 70°C for 30 sec.

Article Title: miRNA-21 enhances chemoresistance to cisplatin in epithelial ovarian cancer by negatively regulating PTEN
Article Snippet: .. RT was performed using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) at 16°C for 30 min, 42°C for 30 min and 85°C for 5 min. qPCR was performed using SYBR Premix Ex Taq (Takara Bio, Inc.) according to the manufacturer's protocol, with 1.33 µl cDNA from the RT reaction. .. The thermocycler conditions were, an initial 15 min at 95°C, then 40 cycles of 15 sec at 95°C and 60 sec at 60°C.

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: .. The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA). .. The total volume of the PCR reaction system was 20 µL.

Article Title: De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes
Article Snippet: Each 20 μL of PCR master mix contained 2 μL of diluted cDNA template, 10 μL of SYBR Premix Ex Taq™, 0.4 μL of forward primer (10 μM), 0.4 μL of reverse primer (10 μM), 0.4 μL of Rox Reference Dye II (50×) and 6.8 μL of RNase-free H2 O, according to the instructions of SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. Thermal cycling was completed on a 7500 Real-time PCR system (Applied Biosystems, USA) using the following program: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Subsequently, a melting curve analysis from 60 °C to 90 °C was applied to all reactions to verify a single PCR product.

Article Title: SNX10 promotes phagosome maturation in macrophages and protects mice against Listeria monocytogenes infection
Article Snippet: Paragraph title: RNA isolation and quantitative PCR (Q-PCR) ... Then, Q-PCR was performed using a 96-well CFX-96 detection system (Bio-Rad Laboratories) with SYBR Premix Ex Taq™ (TaKaRa, Cat. No. DRR041A).

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: qRT-PCR analysis of Of RyR expression The relative expression levels of Of RyR mRNA in different developmental stages were examined by quantitative real-time PCR (qRT-PCR). .. Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China).

Article Title: Autophagy inhibits chemotherapy-induced apoptosis through downregulating Bad and Bim in hepatocellular carcinoma cells
Article Snippet: Paragraph title: RNA isolation, reverse transcription, and quantitative real-time PCR (q-PCR) ... The q-PCR was conducted in a Roche LightCycler480 using the SYBR Premix Ex Taq (TaKaRa, Cat. DRR420A).

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: .. The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols. .. The primer sequences for SCIN gene amplification were as follows: Forward, AGGAAGGTCTGAACTAAT and reverse, CTGTTACTTATGTCTGCTAT.

Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis
Article Snippet: .. Total RNA (500 ng/µl aliquots) were reverse-transcribed into cDNA using a PrimeScript RT Reagent kit (cat. no. DRR037S; Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using the SYBR Premix Ex Taq™ (cat. no. DRR041S; Takara Biotechnology Co., Ltd.). .. The following gene-specific primers were designed and synthesized by Takara Biotechnology Co., Ltd. and used for the qPCR: Col-I forward, 5′-CTCCTGGCAAGAACGGAGATG-3′ and reverse, 5′-CTGTTCCAGGCAATCCACGA-3′; β-actin forward, 5′-CCCGCGAGTACAACCTTCTT-3′ and reverse, 5′-TCATCCATGGCGAACTGGTG-3′.

Concentration Assay:

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. The amplification efficiency of the target gene and housekeeping gene were estimated by using E = (10−1/slope )−1, where the slope was derived from the plot of the cycle threshold (Ct) value versus the log of the serially diluted template concentration.

Polymerase Chain Reaction:

Article Title: Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... RNA was reverse transcribed using the PrimeScript RT reagent kit (cat. no. RR037; Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol, and then processed for qPCR using SYBR Premix Ex Taq (cat. no. RR390; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol.

Article Title: Let-7a inhibits proliferation and promotes apoptosis of human asthmatic airway smooth muscle cells
Article Snippet: This reaction was performed at 25°C for 5 min, 50°C for 20 min then 75°C for 5 min. qPCR was performed with SYBR® Premix Ex Taq™ (Takara Bio Inc., Otsu, Japan; cat. no. DRR041A) according to the manufacturer's instructions, using an ABI 7500 real-time fluorescence qPCR machine (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. The reaction conditions for PCR were as follows: Initial denaturation at 95°C for 3 min and 40 cycles of 95°C for 15 sec and 60°C for 30 sec.

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland). .. The PCR conditions were as follows: Initial denaturation at 95°C for 30 sec; followed by 35 cycles of denaturation at 94°C for 30 sec, annealing at 50°C for 30 sec, and extension at 70°C for 30 sec.

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA). .. The total volume of the PCR reaction system was 20 µL.

Article Title: De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes
Article Snippet: .. Each 20 μL of PCR master mix contained 2 μL of diluted cDNA template, 10 μL of SYBR Premix Ex Taq™, 0.4 μL of forward primer (10 μM), 0.4 μL of reverse primer (10 μM), 0.4 μL of Rox Reference Dye II (50×) and 6.8 μL of RNase-free H2 O, according to the instructions of SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. Thermal cycling was completed on a 7500 Real-time PCR system (Applied Biosystems, USA) using the following program: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Subsequently, a melting curve analysis from 60 °C to 90 °C was applied to all reactions to verify a single PCR product.

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: .. Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. Thermal cycling was completed on a 7500 Real-time PCR System (Applied Biosystems) using the program of 95°C for 30 s, followed by 40 cycles of 95°C for 5 s and 60°C for 34 s. After the cycling protocol, a melting curve analysis from 60°C to 95°C was applied to all reactions to verify a single PCR product.

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: Paragraph title: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols.

Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... Total RNA (500 ng/µl aliquots) were reverse-transcribed into cDNA using a PrimeScript RT Reagent kit (cat. no. DRR037S; Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using the SYBR Premix Ex Taq™ (cat. no. DRR041S; Takara Biotechnology Co., Ltd.).

Construct:

Article Title: Lime and ammonium carbonate fumigation coupled with bio‐organic fertilizer application steered banana rhizosphere to assemble a unique microbiome against Panama disease
Article Snippet: Standard curves were constructed using 10‐fold serial dilutions of a plasmid containing a copy of the ITS fragment from Foc 4. .. Quantitative PCR amplifications were performed in a 20‐μl mixture for each reaction containing 2 μl template DNA, 10 μl SYBR® Premix Ex Taq™ (TaKaRa, Shiga, Ja pan), 0.4 μl of each primer, 0.4 μl ROX Reference Dye II and nuclease‐free water.

Expressing:

Article Title: Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer
Article Snippet: RNA was reverse transcribed using the PrimeScript RT reagent kit (cat. no. RR037; Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol, and then processed for qPCR using SYBR Premix Ex Taq (cat. no. RR390; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. The relative expression levels were calculated and normalized using the 2−ΔΔCq method ( ).

Article Title: CDK11p58 inhibits ERα-positive breast cancer invasion by targeting integrin β3 via the repression of ERα signaling
Article Snippet: .. After converting total RNA to cDNA in a reverse transcription (RT) reaction by Cdna Synthesis Kit (Cat. 6110A, TaKaRa, Dalian, China), qPCR were used to quantitate the mRNA expression levels by SYBR ® Premix Ex Taq™(Cat. RR420Q, TaKaRa). ..

Article Title: Association of BCSC-1 and MMP-14 with human breast cancer
Article Snippet: SYBR Premix Ex Taq™ (cat. no. DRR820s; Takara) was used for qPCR in a LightCycler 480 instrument (Roche, Basel, Switzerland). .. The relative mRNA expression level was determined by normalizing the cycle threshold (Cq) of the gene of interest to that of β-actin using the 2−ΔΔCt formula.

Article Title: miRNA-21 enhances chemoresistance to cisplatin in epithelial ovarian cancer by negatively regulating PTEN
Article Snippet: miRNA-21 and PTEN mRNA expression levels were detected by stem-loop RT-qPCR. .. RT was performed using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) at 16°C for 30 min, 42°C for 30 min and 85°C for 5 min. qPCR was performed using SYBR Premix Ex Taq (Takara Bio, Inc.) according to the manufacturer's protocol, with 1.33 µl cDNA from the RT reaction.

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: .. The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA). .. The total volume of the PCR reaction system was 20 µL.

Article Title: De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes
Article Snippet: Each 20 μL of PCR master mix contained 2 μL of diluted cDNA template, 10 μL of SYBR Premix Ex Taq™, 0.4 μL of forward primer (10 μM), 0.4 μL of reverse primer (10 μM), 0.4 μL of Rox Reference Dye II (50×) and 6.8 μL of RNase-free H2 O, according to the instructions of SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. The housekeeping gene β-actin from the ACB was used as a previously evaluated internal control and was applied as an appropriate reference for insecticide-induced gene expression profiling [ ].

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: Paragraph title: qRT-PCR analysis of Of RyR expression ... Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China).

Article Title: Autophagy inhibits chemotherapy-induced apoptosis through downregulating Bad and Bim in hepatocellular carcinoma cells
Article Snippet: The mRNA expression of the genes was analyzed with specific primers as follows: 5′-GAAGGTGAAGGTCGGAGTC-3′, 5′-GAAGATGGTGATGGGATTTC-3′ for GAPDH, and the length of this amplicon was 225 bp; 5′-CGGAGGATGAGTGACGAGTT-3′, 5′-GATGTGGAGCGAAGGTCACT-3′ for Bad, and the length of this amplicon was 180 bp; 5′-TAAGTTCTGAGTGTGACCGAGA-3′, 5′-GCTCTGTCTGTAGGGAGGTAGG-3′ for Bim, and the length of this amplicon was 96 bp. .. The q-PCR was conducted in a Roche LightCycler480 using the SYBR Premix Ex Taq (TaKaRa, Cat. DRR420A).

Article Title: Scinderin-knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells
Article Snippet: The RT-qPCR reaction conditions were at 95°C for 15 sec, at 95°C for 5 sec, then at 60°C for 30 sec, and the amplification was for 45 cycles using the Real-Time PCR Detection system (cat no. MX3000p; Agilent Technologies, Inc.) with SYBR Premix Ex Taq (cat no. DRR041B, Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocols. .. The relative mRNA expression levels were determined by the cycle threshold (Ct) normalized to GAPDH expression, using the 2−ΔΔCq formula ( ).

Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis
Article Snippet: Quantitative changes in mRNA expression of genes encoding Col-I were assayed by RT-qPCR. .. Total RNA (500 ng/µl aliquots) were reverse-transcribed into cDNA using a PrimeScript RT Reagent kit (cat. no. DRR037S; Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using the SYBR Premix Ex Taq™ (cat. no. DRR041S; Takara Biotechnology Co., Ltd.).

Reverse Transcription Polymerase Chain Reaction:

Article Title: IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production
Article Snippet: Paragraph title: Isolation of RNA, q-PCR, and semi-quantitative RT-PCR ... Then, q-PCR was performed using a 96-well CFX-96 detection system (Bio-Rad Laboratories) with SYBR Premix Ex Taq™ (Takara, Cat. No. DRR041A).

Article Title: CDK11p58 inhibits ERα-positive breast cancer invasion by targeting integrin β3 via the repression of ERα signaling
Article Snippet: Paragraph title: TaqMan® Array human metastasis gene expression assays and quantitative reverse transcription polymerase chain reaction (qRT-PCR) ... After converting total RNA to cDNA in a reverse transcription (RT) reaction by Cdna Synthesis Kit (Cat. 6110A, TaKaRa, Dalian, China), qPCR were used to quantitate the mRNA expression levels by SYBR ® Premix Ex Taq™(Cat. RR420Q, TaKaRa).

Article Title: Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
Article Snippet: .. The RT-PCR analysis of gene expression was performed using primers for IL-6 , IL-8 , TNF-α , TLR4 , NF-κB , HSP70 , ribosomal protein L4 (RPL4 ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) , and the SYBR® Premix Ex Taq™ (Cat. 420A, Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR System (Foster City, CA, USA). .. The total volume of the PCR reaction system was 20 µL.

Article Title: SNX10 promotes phagosome maturation in macrophages and protects mice against Listeria monocytogenes infection
Article Snippet: RNA isolation and quantitative PCR (Q-PCR) Total RNA was extracted using RNAiso Plus (TaKaRa), and reverse transcription (RT) was carried out using a PrimeScript™ RT-PCR kit (TaKaRa). .. Then, Q-PCR was performed using a 96-well CFX-96 detection system (Bio-Rad Laboratories) with SYBR Premix Ex Taq™ (TaKaRa, Cat. No. DRR041A).

Chloramphenicol Acetyltransferase Assay:

Article Title: Autophagy inhibits chemotherapy-induced apoptosis through downregulating Bad and Bim in hepatocellular carcinoma cells
Article Snippet: For the cDNA synthesis, 1 μg of RNA was reverse transcribed with the RT Reagent Kit with gDNA Eraser (TaKaRa, Cat.DRR047S). .. The q-PCR was conducted in a Roche LightCycler480 using the SYBR Premix Ex Taq (TaKaRa, Cat. DRR420A).

Spectrophotometry:

Article Title: Let-7a inhibits proliferation and promotes apoptosis of human asthmatic airway smooth muscle cells
Article Snippet: RT-qPCR Total RNA of was extracted from the cells using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.), and the purity and content of the extracted RNA were determined using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Inc.) and RNA samples with a A260/A280 ratio between 1.8 and 2.0 were used to synthesize cDNA. .. This reaction was performed at 25°C for 5 min, 50°C for 20 min then 75°C for 5 min. qPCR was performed with SYBR® Premix Ex Taq™ (Takara Bio Inc., Otsu, Japan; cat. no. DRR041A) according to the manufacturer's instructions, using an ABI 7500 real-time fluorescence qPCR machine (Applied Biosystems; Thermo Fisher Scientific, Inc.).

Derivative Assay:

Article Title: Molecular Cloning, Characterization and Expression Profiling of a Ryanodine Receptor Gene in Asian Corn Borer, Ostrinia furnacalis (Guen?e)
Article Snippet: Each 20 µL PCR master mix contained 2 µL diluted cDNA template, 10 µL SYBR Premix Ex Taq™, 0.4 µL forward primer (10 uM), 0.4 µL reverse primer (10 uM), 0.4 µL Rox Reference Dye II(50×) and 6.8 µL RNase free H2 O, which follows the instructions of the SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara, Dalian, China). .. The amplification efficiency of the target gene and housekeeping gene were estimated by using E = (10−1/slope )−1, where the slope was derived from the plot of the cycle threshold (Ct) value versus the log of the serially diluted template concentration.

Software:

Article Title: CDK11p58 inhibits ERα-positive breast cancer invasion by targeting integrin β3 via the repression of ERα signaling
Article Snippet: The results were analyzed using the RQ manager software. .. After converting total RNA to cDNA in a reverse transcription (RT) reaction by Cdna Synthesis Kit (Cat. 6110A, TaKaRa, Dalian, China), qPCR were used to quantitate the mRNA expression levels by SYBR ® Premix Ex Taq™(Cat. RR420Q, TaKaRa).

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    TaKaRa sybr premix ex taq ii
    Sybr Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 3775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 3775 article reviews
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