sybr premix ex taq ii  (TaKaRa)


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  • 99
    Name:
    Premix Ex Taq
    Description:

    Catalog Number:
    RR390A
    Price:
    None
    Category:
    qPCR
    Size:
    200 Rxns
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    Structured Review

    TaKaRa sybr premix ex taq ii
    The propagation of cell-adapted PEDV strain LJB/03 P23 in Vero cells (with or without trypsin), Vero/TMPRSS2 and Vero/MSPL cells. Ultrathin sections of PEDV LJB/03-infected Vero cells at 24 h post-infection were prepared, and massive virus particles as shown by the arrow (Bar = 200 nm) were observed by the electron microscopy (a); PEDV LJB/03 particles in culture media as shown by the arrow (Bar = 200 nm) were observed by the transmission electron microscopy (b). (c) Trypsin-dependence of LJB/03 P23 was determined by RT-PCR assay with <t>SYBR</t> Premix EX <t>Taq</t> II (* p

    https://www.bioz.com/result/sybr premix ex taq ii/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr premix ex taq ii - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Establishment of stable Vero cell lines expressing TMPRSS2 and MSPL: A useful tool for propagating porcine epidemic diarrhea virus in the absence of exogenous trypsin"

    Article Title: Establishment of stable Vero cell lines expressing TMPRSS2 and MSPL: A useful tool for propagating porcine epidemic diarrhea virus in the absence of exogenous trypsin

    Journal: Virulence

    doi: 10.1080/21505594.2020.1770491

    The propagation of cell-adapted PEDV strain LJB/03 P23 in Vero cells (with or without trypsin), Vero/TMPRSS2 and Vero/MSPL cells. Ultrathin sections of PEDV LJB/03-infected Vero cells at 24 h post-infection were prepared, and massive virus particles as shown by the arrow (Bar = 200 nm) were observed by the electron microscopy (a); PEDV LJB/03 particles in culture media as shown by the arrow (Bar = 200 nm) were observed by the transmission electron microscopy (b). (c) Trypsin-dependence of LJB/03 P23 was determined by RT-PCR assay with SYBR Premix EX Taq II (* p
    Figure Legend Snippet: The propagation of cell-adapted PEDV strain LJB/03 P23 in Vero cells (with or without trypsin), Vero/TMPRSS2 and Vero/MSPL cells. Ultrathin sections of PEDV LJB/03-infected Vero cells at 24 h post-infection were prepared, and massive virus particles as shown by the arrow (Bar = 200 nm) were observed by the electron microscopy (a); PEDV LJB/03 particles in culture media as shown by the arrow (Bar = 200 nm) were observed by the transmission electron microscopy (b). (c) Trypsin-dependence of LJB/03 P23 was determined by RT-PCR assay with SYBR Premix EX Taq II (* p

    Techniques Used: Infection, Electron Microscopy, Transmission Assay, Reverse Transcription Polymerase Chain Reaction

    2) Product Images from "Inhibition of NADPH oxidase 2 induces apoptosis in osteosarcoma: The role of reactive oxygen species in cell proliferation"

    Article Title: Inhibition of NADPH oxidase 2 induces apoptosis in osteosarcoma: The role of reactive oxygen species in cell proliferation

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8291

    Quantitative analysis of NOX2 and NOX4 mRNA expression in human OS cell lines. Quantitative analysis of the mRNA expression of NOX family proteins in five human OS cell lines was performed by RT-qPCR using a SYBR Green reagent. The Cq value during the exponential phase of amplification was determined by the real-time monitoring of the fluorescent emission of Taq polymerase nuclease activity. β- actin was used as an internal control for normalization. qPCR efficiencies of the target ( NOX2 and NOX4 ) and control (β-actin) proteins were approximately equal over a concentration range of 0.1–200 ng total cDNA. The relative transcripts were determined by the formula: 1/2 (Cq target-Cq control) ). The bars represent the mean (n=2). OS, osteosarcoma; NOX, NADPH oxidase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Cq, quantification cycle; PBMCs, peripheral blood mononuclear cells.
    Figure Legend Snippet: Quantitative analysis of NOX2 and NOX4 mRNA expression in human OS cell lines. Quantitative analysis of the mRNA expression of NOX family proteins in five human OS cell lines was performed by RT-qPCR using a SYBR Green reagent. The Cq value during the exponential phase of amplification was determined by the real-time monitoring of the fluorescent emission of Taq polymerase nuclease activity. β- actin was used as an internal control for normalization. qPCR efficiencies of the target ( NOX2 and NOX4 ) and control (β-actin) proteins were approximately equal over a concentration range of 0.1–200 ng total cDNA. The relative transcripts were determined by the formula: 1/2 (Cq target-Cq control) ). The bars represent the mean (n=2). OS, osteosarcoma; NOX, NADPH oxidase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Cq, quantification cycle; PBMCs, peripheral blood mononuclear cells.

    Techniques Used: Expressing, Quantitative RT-PCR, SYBR Green Assay, Amplification, Activity Assay, Real-time Polymerase Chain Reaction, Concentration Assay

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    Concentration Assay:

    Article Title: Increasing transforming growth factor-beta concentrations with age decrease apelin in the rat rotator cuff
    Article Snippet: .. First-strand cDNA synthesis was conducted using SuperScript III RT (Invitrogen) with the extracted RNA as a template, 2 μL cDNA, a specific primer set (0.2 μM final concentration), and 12.5 μL SYBR Premix Ex Taq (Takara, Shiga, Japan) in a total volume of 25 μL. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Advanced baseline immunosuppression is associated with elevated levels of plasma markers of fungal translocation and inflammation in long-term treated HIV-infected Tanzanians
    Article Snippet: .. Quantitative real-time PCR analyses was carried out with Premix Ex Taq (Takara-bio, Japan) and qPCR reactions was performed by using LightCycler 96 System (Roche Diagnostics GmbH, Mannheim, Germany). ..

    Article Title: A MYB transcription factor, PlMYB308, plays an essential role in flower senescence of herbaceous peony
    Article Snippet: .. SYBR Premix Ex Taq II (Takara, Otsu, Shiga, Japan) on StepOnePlus Real Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) was used for the qRT-PCR assay. ..

    Article Title: BMSC-Derived Exosomal MiRNAs Can Modulate Bone Restoration in Diabetic Rats with Femoral Defects
    Article Snippet: .. Quantitative real-time PCR (qPCR) was performed using SYBR® Premix Ex Taq™ (TaKaRa) in a Bio-Rad CFX96™ real-time PCR system using the following thermocycling conditions: pre-denaturation at 95 °C for 5 s, followed by 40 cycles of denaturation at 95 °C, 10 s; annealing at 57 °C, 20 s; and extension at 72 °C, 20 s. The relative expression of the specified genes was calculated using the 2−ΔΔCT method after normalization to GAPDH expression. ..

    Article Title: Prognostic role of DFNA5 in head and neck squamous cell carcinoma revealed by systematic expression analysis
    Article Snippet: .. RNA was then converted to cDNA using PrimeScript RT Master Mix (Takara). qPCR was performed on a Bio-Rad Thermal Cycler using SYBR Premix Ex Taq II (Takara) with primers against human DFNA5. ..

    Article Title: Synergistic interaction of hyposalinity stress with Vibrio infection causes mass mortalities in oysters by inducing host microflora imbalance and immune dysregulation
    Article Snippet: .. The amplification was performed on the LightCycler 480 real-time PCR instrument (Roche Diagnostics, Burgess Hill, UK) using SYBR® Premix Ex Taq™ (TaKaRa). ..

    Quantitative RT-PCR:

    Article Title: A MYB transcription factor, PlMYB308, plays an essential role in flower senescence of herbaceous peony
    Article Snippet: .. SYBR Premix Ex Taq II (Takara, Otsu, Shiga, Japan) on StepOnePlus Real Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) was used for the qRT-PCR assay. ..

    Expressing:

    Article Title: BMSC-Derived Exosomal MiRNAs Can Modulate Bone Restoration in Diabetic Rats with Femoral Defects
    Article Snippet: .. Quantitative real-time PCR (qPCR) was performed using SYBR® Premix Ex Taq™ (TaKaRa) in a Bio-Rad CFX96™ real-time PCR system using the following thermocycling conditions: pre-denaturation at 95 °C for 5 s, followed by 40 cycles of denaturation at 95 °C, 10 s; annealing at 57 °C, 20 s; and extension at 72 °C, 20 s. The relative expression of the specified genes was calculated using the 2−ΔΔCT method after normalization to GAPDH expression. ..

    Article Title: Temozolomide Drives Ferroptosis via a DMT1-Dependent Pathway in Glioblastoma Cells
    Article Snippet: .. The mRNA expression levels were detected by qPCR with an Applied Biosystems 7500 Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA) with SYBR Premix Ex Taq (Takara) according to the manufacturer's instructions and were normalized versus GAPDH mRNA. ..

    Amplification:

    Article Title: Synergistic interaction of hyposalinity stress with Vibrio infection causes mass mortalities in oysters by inducing host microflora imbalance and immune dysregulation
    Article Snippet: .. The amplification was performed on the LightCycler 480 real-time PCR instrument (Roche Diagnostics, Burgess Hill, UK) using SYBR® Premix Ex Taq™ (TaKaRa). ..

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  • 98
    TaKaRa pcr master mix
    cDNA sequence of mRNA de novo transcribed from <t>TNFSF9</t> in HepG2 clone 9-1 (A) HepG2 clone 9-1 cells were incubated with 500 ng/ml LPS for the indicated times. cDNA was synthesized from total RNA, and TNFSF9 expression was quantified by real-time <t>PCR</t> as described in “Materials and Methods”. (B) HepG2 cells were incubated with 500 ng/ml LPS in the presence of 5-ethynyl uridine (EU) for 16 hrs. EU-labeled RNA was isolated as described in “Materials and Methods”. TNFSF9 cDNA was amplified by PCR and the PCR products were isolated and sequenced. This experiment is representative of four independent experiments.
    Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/TaKaRa
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2021-09
    98/100 stars
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    99
    TaKaRa sybr premix ex taq ii kit
    Expression levels of miR-192, -194 and -215 in three colorectal cancer (CRC) cell lines (HT-29, HCT-116 and SW-620). Quantification of miRNAs was measured by <t>SYBR</t> Premix Ex <t>Taq</t> II. Data are presented in CRC cell lines relative to normal colorectal tissues
    Sybr Premix Ex Taq Ii Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr premix ex taq ii kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr premix ex taq ii kit - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    cDNA sequence of mRNA de novo transcribed from TNFSF9 in HepG2 clone 9-1 (A) HepG2 clone 9-1 cells were incubated with 500 ng/ml LPS for the indicated times. cDNA was synthesized from total RNA, and TNFSF9 expression was quantified by real-time PCR as described in “Materials and Methods”. (B) HepG2 cells were incubated with 500 ng/ml LPS in the presence of 5-ethynyl uridine (EU) for 16 hrs. EU-labeled RNA was isolated as described in “Materials and Methods”. TNFSF9 cDNA was amplified by PCR and the PCR products were isolated and sequenced. This experiment is representative of four independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: cDNA sequence of mRNA de novo transcribed from TNFSF9 in HepG2 clone 9-1 (A) HepG2 clone 9-1 cells were incubated with 500 ng/ml LPS for the indicated times. cDNA was synthesized from total RNA, and TNFSF9 expression was quantified by real-time PCR as described in “Materials and Methods”. (B) HepG2 cells were incubated with 500 ng/ml LPS in the presence of 5-ethynyl uridine (EU) for 16 hrs. EU-labeled RNA was isolated as described in “Materials and Methods”. TNFSF9 cDNA was amplified by PCR and the PCR products were isolated and sequenced. This experiment is representative of four independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Sequencing, Incubation, Synthesized, Expressing, Real-time Polymerase Chain Reaction, Labeling, Isolation, Amplification, Polymerase Chain Reaction

    DNA sequence of genomic TNFSF9 gene edited by CRISPR-Cas9 The TNFSF9 genes in genomic DNAs from wild type and mutated HepG2 cells were amplified by PCR. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the genomic TNFSF9 sequence. On the right are shown the DNA sequences around the region of the mutated triplet in the wildtype and mutant clones. This is one representative of five independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: DNA sequence of genomic TNFSF9 gene edited by CRISPR-Cas9 The TNFSF9 genes in genomic DNAs from wild type and mutated HepG2 cells were amplified by PCR. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the genomic TNFSF9 sequence. On the right are shown the DNA sequences around the region of the mutated triplet in the wildtype and mutant clones. This is one representative of five independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Sequencing, CRISPR, Amplification, Polymerase Chain Reaction, Isolation, Mutagenesis, Clone Assay

    Heteroduplex formation by the cDNA of de novo transcripts of TNFSF9 from HepG2 clone 9-1 Total RNA and EU-labeled RNAs were isolated from clone 9-1 HepG2 cells. cDNA was synthesized and the TNFSF9 cDNA was amplified by PCR as described in “Materials and Methods”. The PCR products were annealed and incubated in the presence or absence of T7E1 endonuclease, and the products were fractionated on 1.2% agarose gels and visualized under UV. This is one representative of three independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: Heteroduplex formation by the cDNA of de novo transcripts of TNFSF9 from HepG2 clone 9-1 Total RNA and EU-labeled RNAs were isolated from clone 9-1 HepG2 cells. cDNA was synthesized and the TNFSF9 cDNA was amplified by PCR as described in “Materials and Methods”. The PCR products were annealed and incubated in the presence or absence of T7E1 endonuclease, and the products were fractionated on 1.2% agarose gels and visualized under UV. This is one representative of three independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Labeling, Isolation, Synthesized, Amplification, Polymerase Chain Reaction, Incubation

    cDNA sequence of mRNA transcribed from mutant genomic TNFSF9 TNFSF9 cDNA was synthesized and amplified by PCR from total RNA extracted from wild type and mutated HepG2 cells. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the TNFSF9 cDNA sequences. On the right are shown the cDNA sequences around the region of the mutated triplet in the wild type and mutant clones. This is one representative of four independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: cDNA sequence of mRNA transcribed from mutant genomic TNFSF9 TNFSF9 cDNA was synthesized and amplified by PCR from total RNA extracted from wild type and mutated HepG2 cells. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the TNFSF9 cDNA sequences. On the right are shown the cDNA sequences around the region of the mutated triplet in the wild type and mutant clones. This is one representative of four independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Sequencing, Mutagenesis, Synthesized, Amplification, Polymerase Chain Reaction, Isolation, Clone Assay

    Expression levels of miR-192, -194 and -215 in three colorectal cancer (CRC) cell lines (HT-29, HCT-116 and SW-620). Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Data are presented in CRC cell lines relative to normal colorectal tissues

    Journal: Experimental and Therapeutic Medicine

    Article Title: microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

    doi: 10.3892/etm.2011.436

    Figure Lengend Snippet: Expression levels of miR-192, -194 and -215 in three colorectal cancer (CRC) cell lines (HT-29, HCT-116 and SW-620). Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Data are presented in CRC cell lines relative to normal colorectal tissues

    Article Snippet: According to the manufacturer's instructions, real-time PCR was performed using the SYBR Premix Ex Taq™ II kit (Takara Bio, Kyoto, Japan) with a Rotor-gene 6000 system (Qiagen, Valencia, CA, USA) ( ).

    Techniques: Expressing

    Expression levels of miR-192, -194 and -215 in 107 patients with colorectal cancer (CRC). (A, C and E) Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Each sample was analyzed in triplicate and repeated three times. Data are presented

    Journal: Experimental and Therapeutic Medicine

    Article Title: microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

    doi: 10.3892/etm.2011.436

    Figure Lengend Snippet: Expression levels of miR-192, -194 and -215 in 107 patients with colorectal cancer (CRC). (A, C and E) Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Each sample was analyzed in triplicate and repeated three times. Data are presented

    Article Snippet: According to the manufacturer's instructions, real-time PCR was performed using the SYBR Premix Ex Taq™ II kit (Takara Bio, Kyoto, Japan) with a Rotor-gene 6000 system (Qiagen, Valencia, CA, USA) ( ).

    Techniques: Expressing

    The propagation of cell-adapted PEDV strain LJB/03 P23 in Vero cells (with or without trypsin), Vero/TMPRSS2 and Vero/MSPL cells. Ultrathin sections of PEDV LJB/03-infected Vero cells at 24 h post-infection were prepared, and massive virus particles as shown by the arrow (Bar = 200 nm) were observed by the electron microscopy (a); PEDV LJB/03 particles in culture media as shown by the arrow (Bar = 200 nm) were observed by the transmission electron microscopy (b). (c) Trypsin-dependence of LJB/03 P23 was determined by RT-PCR assay with SYBR Premix EX Taq II (* p

    Journal: Virulence

    Article Title: Establishment of stable Vero cell lines expressing TMPRSS2 and MSPL: A useful tool for propagating porcine epidemic diarrhea virus in the absence of exogenous trypsin

    doi: 10.1080/21505594.2020.1770491

    Figure Lengend Snippet: The propagation of cell-adapted PEDV strain LJB/03 P23 in Vero cells (with or without trypsin), Vero/TMPRSS2 and Vero/MSPL cells. Ultrathin sections of PEDV LJB/03-infected Vero cells at 24 h post-infection were prepared, and massive virus particles as shown by the arrow (Bar = 200 nm) were observed by the electron microscopy (a); PEDV LJB/03 particles in culture media as shown by the arrow (Bar = 200 nm) were observed by the transmission electron microscopy (b). (c) Trypsin-dependence of LJB/03 P23 was determined by RT-PCR assay with SYBR Premix EX Taq II (* p

    Article Snippet: Real-time polymerase chain reaction (RT-PCR) analysisTo analyze the trypsin-dependence of PEDV LJB/03 P23 and PEDV isolates 2013-A and NJ, the copy number of viral RNA was determined by RT-PCR assay with SYBR Premix EX Taq II (Takara) and ABI 7500 real-time PCR system (Applied Biosystems, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Techniques: Infection, Electron Microscopy, Transmission Assay, Reverse Transcription Polymerase Chain Reaction

    Quantitative analysis of NOX2 and NOX4 mRNA expression in human OS cell lines. Quantitative analysis of the mRNA expression of NOX family proteins in five human OS cell lines was performed by RT-qPCR using a SYBR Green reagent. The Cq value during the exponential phase of amplification was determined by the real-time monitoring of the fluorescent emission of Taq polymerase nuclease activity. β- actin was used as an internal control for normalization. qPCR efficiencies of the target ( NOX2 and NOX4 ) and control (β-actin) proteins were approximately equal over a concentration range of 0.1–200 ng total cDNA. The relative transcripts were determined by the formula: 1/2 (Cq target-Cq control) ). The bars represent the mean (n=2). OS, osteosarcoma; NOX, NADPH oxidase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Cq, quantification cycle; PBMCs, peripheral blood mononuclear cells.

    Journal: Oncology Letters

    Article Title: Inhibition of NADPH oxidase 2 induces apoptosis in osteosarcoma: The role of reactive oxygen species in cell proliferation

    doi: 10.3892/ol.2018.8291

    Figure Lengend Snippet: Quantitative analysis of NOX2 and NOX4 mRNA expression in human OS cell lines. Quantitative analysis of the mRNA expression of NOX family proteins in five human OS cell lines was performed by RT-qPCR using a SYBR Green reagent. The Cq value during the exponential phase of amplification was determined by the real-time monitoring of the fluorescent emission of Taq polymerase nuclease activity. β- actin was used as an internal control for normalization. qPCR efficiencies of the target ( NOX2 and NOX4 ) and control (β-actin) proteins were approximately equal over a concentration range of 0.1–200 ng total cDNA. The relative transcripts were determined by the formula: 1/2 (Cq target-Cq control) ). The bars represent the mean (n=2). OS, osteosarcoma; NOX, NADPH oxidase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Cq, quantification cycle; PBMCs, peripheral blood mononuclear cells.

    Article Snippet: RT-qPCR was performed with SYBR Premix Ex Taq II (Takara Bio, Inc., Otsu, Shiga, Japan) in an ABI PRISM 7500 Sequence Detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay, Amplification, Activity Assay, Real-time Polymerase Chain Reaction, Concentration Assay