sybr pcr polymerase chain reaction master mix  (Thermo Fisher)


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    Thermo Fisher sybr pcr polymerase chain reaction master mix
    Sybr Pcr Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr pcr polymerase chain reaction master mix/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr pcr polymerase chain reaction master mix - by Bioz Stars, 2020-08
    85/100 stars

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    Polymerase Chain Reaction:

    Article Title: Identification of a stem-like cell population by exposing metastatic breast cancer cell lines to repetitive cycles of hypoxia and reoxygenation
    Article Snippet: .. The resulting cDNAs were mixed with the SYBR PCR [polymerase chain reaction] master mix (Applied Biosystems) and run on the StepOnePlus Applied Biosystems Real-time PCR machine. .. One cycle of denaturing step (10 minutes at 95°C) was applied, followed by 35 cycles of amplification (15 seconds at 95°C and 1 minute at 58°C), with fluorescence measured during the extension.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of a stem-like cell population by exposing metastatic breast cancer cell lines to repetitive cycles of hypoxia and reoxygenation
    Article Snippet: .. The resulting cDNAs were mixed with the SYBR PCR [polymerase chain reaction] master mix (Applied Biosystems) and run on the StepOnePlus Applied Biosystems Real-time PCR machine. .. One cycle of denaturing step (10 minutes at 95°C) was applied, followed by 35 cycles of amplification (15 seconds at 95°C and 1 minute at 58°C), with fluorescence measured during the extension.

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    Thermo Fisher sybr green i
    Correlation of detection of virus by Bio-Plex and RT-qPCR. Correlation between qPCR (Ct value) and Bio-Plex (MFI) detection of in-vitro grown ISAV standard curve by (A) <t>SYBR</t> <t>GREEN</t> I and (B)TaqMan; Correlation of detection of copy number of ISAV in plasma samples by (C) SYBR Green I (n = 13) and (D) TaqMan chemistries (n = 8).
    Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 13018 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2020-08
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    99
    Thermo Fisher fast sybr green master mix
    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with <t>SYBR</t> ™ Safe DNA Gel Stain in agarose gels. (C) <t>qRT-PCR</t> was performed to confirm the results in (B).
    Fast Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fast sybr green master mix - by Bioz Stars, 2020-08
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    Thermo Fisher real time quantitative pcr analysis
    Confirmation of RNA and protein expression levels by real-time quantitative <t>PCR</t> and western blotting analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blotting analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t -test for independent samples. * p
    Real Time Quantitative Pcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative pcr analysis/product/Thermo Fisher
    Average 99 stars, based on 266 article reviews
    Price from $9.99 to $1999.99
    real time quantitative pcr analysis - by Bioz Stars, 2020-08
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    Image Search Results


    Correlation of detection of virus by Bio-Plex and RT-qPCR. Correlation between qPCR (Ct value) and Bio-Plex (MFI) detection of in-vitro grown ISAV standard curve by (A) SYBR GREEN I and (B)TaqMan; Correlation of detection of copy number of ISAV in plasma samples by (C) SYBR Green I (n = 13) and (D) TaqMan chemistries (n = 8).

    Journal: PLoS ONE

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology

    doi: 10.1371/journal.pone.0159155

    Figure Lengend Snippet: Correlation of detection of virus by Bio-Plex and RT-qPCR. Correlation between qPCR (Ct value) and Bio-Plex (MFI) detection of in-vitro grown ISAV standard curve by (A) SYBR GREEN I and (B)TaqMan; Correlation of detection of copy number of ISAV in plasma samples by (C) SYBR Green I (n = 13) and (D) TaqMan chemistries (n = 8).

    Article Snippet: 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix.

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction, In Vitro, SYBR Green Assay

    RT-qPCR standard curves. Standard curve relating viral copy number to Ct value from RT-Polymerase chain reaction using 10-fold dilutions of in-vitro transcribed RNA using (a) SYBR ® Green I and (b) TaqMan chemistries.

    Journal: PLoS ONE

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology

    doi: 10.1371/journal.pone.0159155

    Figure Lengend Snippet: RT-qPCR standard curves. Standard curve relating viral copy number to Ct value from RT-Polymerase chain reaction using 10-fold dilutions of in-vitro transcribed RNA using (a) SYBR ® Green I and (b) TaqMan chemistries.

    Article Snippet: 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix.

    Techniques: Quantitative RT-PCR, Polymerase Chain Reaction, In Vitro, SYBR Green Assay

    Silencing of Snail in Caki-2 by a lentivirus-delivered short inhibitory RNA. A. Map of the lentivirualRNAi vectors [pCMV-G NR-U6-shRNA]. U6, polymerase III promoter to drive the transcription of shRNAs; GFP (green fluorescent protein) were driven by CMV promoter. shRNAs were inserted into HindIII and BamHI sites. B. Strategy plan for generating Snail shRNA constructs. pCMV-G NR-U6 vector was linearized by HindIII and BamH1, and then designed shRNAs were inserted into this site. The positive colonies were screened by colony PCR using the primers flanking the inserted site of shRNA. Selected Colonies were finally confirmed by sequencing. C. Quantitative real-time RT-PCR compare the knock-down efficiency of 3 Snail shRNAs in Caki-2 cells. RNAs were isolated from cells and gene reverse transcription was performed. Quantitative PCR was performed by using SYBR Green PCR Master Mix. Data were normalized to GAPDH mRNA levels, by using efficiency (2 -ΔΔCt ) method. The data were analyzed by student t -test, and were presented as the means ± s.e.m (n = 3). D. Western blotting assay confirmed that effectiveness of Snail shRNA viruses on protein level.The intensity of bands were determined by Image J, and ratio of Snail to GAPDH were shown here.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways

    doi:

    Figure Lengend Snippet: Silencing of Snail in Caki-2 by a lentivirus-delivered short inhibitory RNA. A. Map of the lentivirualRNAi vectors [pCMV-G NR-U6-shRNA]. U6, polymerase III promoter to drive the transcription of shRNAs; GFP (green fluorescent protein) were driven by CMV promoter. shRNAs were inserted into HindIII and BamHI sites. B. Strategy plan for generating Snail shRNA constructs. pCMV-G NR-U6 vector was linearized by HindIII and BamH1, and then designed shRNAs were inserted into this site. The positive colonies were screened by colony PCR using the primers flanking the inserted site of shRNA. Selected Colonies were finally confirmed by sequencing. C. Quantitative real-time RT-PCR compare the knock-down efficiency of 3 Snail shRNAs in Caki-2 cells. RNAs were isolated from cells and gene reverse transcription was performed. Quantitative PCR was performed by using SYBR Green PCR Master Mix. Data were normalized to GAPDH mRNA levels, by using efficiency (2 -ΔΔCt ) method. The data were analyzed by student t -test, and were presented as the means ± s.e.m (n = 3). D. Western blotting assay confirmed that effectiveness of Snail shRNA viruses on protein level.The intensity of bands were determined by Image J, and ratio of Snail to GAPDH were shown here.

    Article Snippet: Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System.

    Techniques: shRNA, Construct, Plasmid Preparation, Polymerase Chain Reaction, Sequencing, Quantitative RT-PCR, Isolation, Real-time Polymerase Chain Reaction, SYBR Green Assay, Western Blot

    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Journal: Heart rhythm

    Article Title: HuR-mediated SCN5A mRNA stability reduces arrhythmic risk in heart failure

    doi: 10.1016/j.hrthm.2018.02.018

    Figure Lengend Snippet: HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Article Snippet: Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out using gene-specific primers, Fast SYBR® Green Master Mix and 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Isolation, Synthesized, Polymerase Chain Reaction, Staining, Quantitative RT-PCR

    Confirmation of RNA and protein expression levels by real-time quantitative PCR and western blotting analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blotting analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t -test for independent samples. * p

    Journal: Proteome Science

    Article Title: Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells

    doi: 10.1186/1477-5956-12-13

    Figure Lengend Snippet: Confirmation of RNA and protein expression levels by real-time quantitative PCR and western blotting analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blotting analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t -test for independent samples. * p

    Article Snippet: The cDNA was then used as the template for real-time quantitative PCR analysis (Maxima SYBR Green/ROX qPCR Master Mix; Thermo Scientific, Pittsburgh, PA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot