sybr master mix  (Thermo Fisher)


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    Name:
    SYBR Select Master Mix
    Description:
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis SYBR Select Master Mix is a superior and cost effective solution for your real time PCR applications Features of the SYBR Select Master Mix include • Specific minimize primer dimer and non specific amplification• Reproducible and sensitive consistent amplification across a wide dynamic range• Bright contains SYBR GreenER dye for maximum brightness• Carry over contamination control contains heat labile UDG• Can be used in either standard or fast cycling modeFormulated for Maximum SpecificitySYBR Select Master Mix contains all the components needed for your real time PCR reaction except the template and primers in a convenient 2X concentration premix The master mix includes AmpliTaq DNA Polymerase UP a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity Obtain Reproducible Results Across a Wide Dynamic RangeSYBR Select Master Mix is specially formulated to provide robust results from 100 ng to 0 1 pg cDNA per reaction Figure 1 and can reliably detect a single copy of genomic DNA Figure 2 Contains SYBR GreenER Dye and Heat labile UDG• SYBR GreenER dye is less inhibitory to PCR than SYBR Green I dye resulting in brighter signals• Heat labile uracil DNA glycosylase UDG is included for worry free carryover contamination controlInstrument CompatibilitySYBR Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real time PCR instruments except the 7900HT real time PCR system It is also compatible with the Bio Rad IQ 5 Roche LightCycler LC480 and Stratagene MX3005P systems View performance data
    Catalog Number:
    4472903
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    Applications:
    Enzymes & Master Mixes for Real-Time PCR|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Gene Expression Analysis & Genotyping
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    Structured Review

    Thermo Fisher sybr master mix
    HEV induces <t>ISG15</t> both in vitro and in vivo . (A) Replication kinetics of viral RNAs from the HEV P6 infectious cDNA clone and HEV P6GLuc replicon in Huh7-S10-3 liver cells. The cells were transfected with capped RNA transcripts from the genotype 3 HEV P6 infectious cDNA clone (HEV P6) or with the HEV P6GLuc replicon (HEV P6GLuc; P6 encoding Gaussia luciferase clone). The culture supernatants were collected at various time points and used to measure viral RNA replication levels by HEV real-time quantitative RT-PCR or by the Gaussia luciferase assay (GLuc assay). RLU, relative light units. (B) ISG15 mRNA expression levels in Huh7-S10-3 liver cells transfected with capped RNA transcripts from the HEV P6 infectious cDNA clone or the HEV P6GLuc replicon at 1 and 5 days posttransfection (dpt) were determined by <t>Sybr</t> green-based quantitative PCR (qPCR). The ISG15-mRNA fold change was calculated using the 2 −ΔΔ CT method for comparisons to untransfected control cells, and the RPS18 gene was used as the housekeeping gene. (C and D) ISG15 protein expression levels in Huh7-S10-3 cells transfected with capped RNA from the HEV P6 infectious cDNA clone or the HEV P6GLuc replicon (C and D) or the HEV P6 replication-deficient infectious cDNA clone (P6GAD) (D). CC, untransfected cell control. The ISG15 protein expression, at 5 days posttransfection, in cell lysates (40 μg/lane) was analyzed by Western blotting using anti-ISG15 antibody (1:1,000 dilution). Fold change in band density, as calculated via normalization of ISG15 with β-actin (loading control), was measured using ImageJ software (NIH, Bethesda, MD). (E) ISG15 and USP18 mRNA expression levels in swine liver tissue samples obtained from animals experimentally infected with HEV ( n = 3) at 3 weeks postinfection. The fold changes in swine ISG15 and USP18 mRNA levels were calculated using the 2 −ΔΔ CT method compared to the uninfected control animals ( n = 2), and the RPS18 gene was used as the housekeeping gene. The data represent means ± standard errors of the means (SEM) of results from three independent transfection experiments (A, B, and C), one experiment representative of two independent transfection experiments (D), and HEV-infected animals ( n = 3) compared to uninfected animals ( n = 2) (E). **, P ≤ 0.01.
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis SYBR Select Master Mix is a superior and cost effective solution for your real time PCR applications Features of the SYBR Select Master Mix include • Specific minimize primer dimer and non specific amplification• Reproducible and sensitive consistent amplification across a wide dynamic range• Bright contains SYBR GreenER dye for maximum brightness• Carry over contamination control contains heat labile UDG• Can be used in either standard or fast cycling modeFormulated for Maximum SpecificitySYBR Select Master Mix contains all the components needed for your real time PCR reaction except the template and primers in a convenient 2X concentration premix The master mix includes AmpliTaq DNA Polymerase UP a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity Obtain Reproducible Results Across a Wide Dynamic RangeSYBR Select Master Mix is specially formulated to provide robust results from 100 ng to 0 1 pg cDNA per reaction Figure 1 and can reliably detect a single copy of genomic DNA Figure 2 Contains SYBR GreenER Dye and Heat labile UDG• SYBR GreenER dye is less inhibitory to PCR than SYBR Green I dye resulting in brighter signals• Heat labile uracil DNA glycosylase UDG is included for worry free carryover contamination controlInstrument CompatibilitySYBR Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real time PCR instruments except the 7900HT real time PCR system It is also compatible with the Bio Rad IQ 5 Roche LightCycler LC480 and Stratagene MX3005P systems View performance data
    https://www.bioz.com/result/sybr master mix/product/Thermo Fisher
    Average 99 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    sybr master mix - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication"

    Article Title: ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication

    Journal: Journal of Virology

    doi: 10.1128/JVI.00621-17

    HEV induces ISG15 both in vitro and in vivo . (A) Replication kinetics of viral RNAs from the HEV P6 infectious cDNA clone and HEV P6GLuc replicon in Huh7-S10-3 liver cells. The cells were transfected with capped RNA transcripts from the genotype 3 HEV P6 infectious cDNA clone (HEV P6) or with the HEV P6GLuc replicon (HEV P6GLuc; P6 encoding Gaussia luciferase clone). The culture supernatants were collected at various time points and used to measure viral RNA replication levels by HEV real-time quantitative RT-PCR or by the Gaussia luciferase assay (GLuc assay). RLU, relative light units. (B) ISG15 mRNA expression levels in Huh7-S10-3 liver cells transfected with capped RNA transcripts from the HEV P6 infectious cDNA clone or the HEV P6GLuc replicon at 1 and 5 days posttransfection (dpt) were determined by Sybr green-based quantitative PCR (qPCR). The ISG15-mRNA fold change was calculated using the 2 −ΔΔ CT method for comparisons to untransfected control cells, and the RPS18 gene was used as the housekeeping gene. (C and D) ISG15 protein expression levels in Huh7-S10-3 cells transfected with capped RNA from the HEV P6 infectious cDNA clone or the HEV P6GLuc replicon (C and D) or the HEV P6 replication-deficient infectious cDNA clone (P6GAD) (D). CC, untransfected cell control. The ISG15 protein expression, at 5 days posttransfection, in cell lysates (40 μg/lane) was analyzed by Western blotting using anti-ISG15 antibody (1:1,000 dilution). Fold change in band density, as calculated via normalization of ISG15 with β-actin (loading control), was measured using ImageJ software (NIH, Bethesda, MD). (E) ISG15 and USP18 mRNA expression levels in swine liver tissue samples obtained from animals experimentally infected with HEV ( n = 3) at 3 weeks postinfection. The fold changes in swine ISG15 and USP18 mRNA levels were calculated using the 2 −ΔΔ CT method compared to the uninfected control animals ( n = 2), and the RPS18 gene was used as the housekeeping gene. The data represent means ± standard errors of the means (SEM) of results from three independent transfection experiments (A, B, and C), one experiment representative of two independent transfection experiments (D), and HEV-infected animals ( n = 3) compared to uninfected animals ( n = 2) (E). **, P ≤ 0.01.
    Figure Legend Snippet: HEV induces ISG15 both in vitro and in vivo . (A) Replication kinetics of viral RNAs from the HEV P6 infectious cDNA clone and HEV P6GLuc replicon in Huh7-S10-3 liver cells. The cells were transfected with capped RNA transcripts from the genotype 3 HEV P6 infectious cDNA clone (HEV P6) or with the HEV P6GLuc replicon (HEV P6GLuc; P6 encoding Gaussia luciferase clone). The culture supernatants were collected at various time points and used to measure viral RNA replication levels by HEV real-time quantitative RT-PCR or by the Gaussia luciferase assay (GLuc assay). RLU, relative light units. (B) ISG15 mRNA expression levels in Huh7-S10-3 liver cells transfected with capped RNA transcripts from the HEV P6 infectious cDNA clone or the HEV P6GLuc replicon at 1 and 5 days posttransfection (dpt) were determined by Sybr green-based quantitative PCR (qPCR). The ISG15-mRNA fold change was calculated using the 2 −ΔΔ CT method for comparisons to untransfected control cells, and the RPS18 gene was used as the housekeeping gene. (C and D) ISG15 protein expression levels in Huh7-S10-3 cells transfected with capped RNA from the HEV P6 infectious cDNA clone or the HEV P6GLuc replicon (C and D) or the HEV P6 replication-deficient infectious cDNA clone (P6GAD) (D). CC, untransfected cell control. The ISG15 protein expression, at 5 days posttransfection, in cell lysates (40 μg/lane) was analyzed by Western blotting using anti-ISG15 antibody (1:1,000 dilution). Fold change in band density, as calculated via normalization of ISG15 with β-actin (loading control), was measured using ImageJ software (NIH, Bethesda, MD). (E) ISG15 and USP18 mRNA expression levels in swine liver tissue samples obtained from animals experimentally infected with HEV ( n = 3) at 3 weeks postinfection. The fold changes in swine ISG15 and USP18 mRNA levels were calculated using the 2 −ΔΔ CT method compared to the uninfected control animals ( n = 2), and the RPS18 gene was used as the housekeeping gene. The data represent means ± standard errors of the means (SEM) of results from three independent transfection experiments (A, B, and C), one experiment representative of two independent transfection experiments (D), and HEV-infected animals ( n = 3) compared to uninfected animals ( n = 2) (E). **, P ≤ 0.01.

    Techniques Used: In Vitro, In Vivo, Transfection, Luciferase, Quantitative RT-PCR, Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction, Western Blot, Software, Infection

    ISG15 regulates type I IFN signaling. (A) Relative pSTAT1 levels. Huh7-S10-3 liver cells were transfected with 20 nM control siRNA (siCnt) or ISG15-siRNA (siISG15) or UBE1L-siRNA plus UBE2L6-siRNA (siE1E2). At 24 hpt, cells were treated with IFN-α (100 IU/ml) for 30 min to 7 h. Cell lysate (20 μg/lane) was analyzed by Western blotting with the indicated antibody, anti-pSTAT1 (1:1,000 dilution), anti-STAT1 (1:1,000 dilution), and anti-β-actin (1:1,000 dilution). Fold change in band intensity was determined using ImageJ software (NIH, Bethesda, MD). The data represent means ± SEM of results from five independent experiments. a, P ≤ 0.05; aa, P ≤ 0.01 (compared with siCnt plus IFN-α at the given time point). (B) IFN-stimulated response element (ISRE) promoter activity levels. Huh7-S10-3 liver cells were cotransfected with siCnt/siISG15/siE1E2 along with pGL4.45[luc2P/ISRE/Hygro] (firefly luciferase) and pGL4.74[hRluc/TK] (renilla luciferase). At 24 hpt, cells were treated with various concentrations of IFN-α. Relative levels of fold induction of the cell-associated firefly luciferase activity, compared to the corresponding untreated cell control levels, at 18 h post-IFN-α treatment were estimated using a dual-luciferase assay kit and were normalized with renilla luciferase expression levels. The data represent means ± SEM of results from triplicate sample experiments. aa, P ≤ 0.01 (compared to siCnt plus IFN-α). (C to F) ISG mRNA levels in siRNA-transfected and IFN-α-treated samples were measured for Mx1 (C), OAS1 (D), PKR (E), and ISG15 (F) using Sybr green qPCR. Fold change in mRNA levels compared to the untransfected control was calculated using the 2 −ΔΔ CT method, and the RPS18 gene was used as the housekeeping gene. The data represent means ± SEM of results from three independent experiments. a, P ≤ 0.05; aa, P ≤ 0.01; aaa, P ≤ 0.001 (compared to siCnt plus IFN-α).
    Figure Legend Snippet: ISG15 regulates type I IFN signaling. (A) Relative pSTAT1 levels. Huh7-S10-3 liver cells were transfected with 20 nM control siRNA (siCnt) or ISG15-siRNA (siISG15) or UBE1L-siRNA plus UBE2L6-siRNA (siE1E2). At 24 hpt, cells were treated with IFN-α (100 IU/ml) for 30 min to 7 h. Cell lysate (20 μg/lane) was analyzed by Western blotting with the indicated antibody, anti-pSTAT1 (1:1,000 dilution), anti-STAT1 (1:1,000 dilution), and anti-β-actin (1:1,000 dilution). Fold change in band intensity was determined using ImageJ software (NIH, Bethesda, MD). The data represent means ± SEM of results from five independent experiments. a, P ≤ 0.05; aa, P ≤ 0.01 (compared with siCnt plus IFN-α at the given time point). (B) IFN-stimulated response element (ISRE) promoter activity levels. Huh7-S10-3 liver cells were cotransfected with siCnt/siISG15/siE1E2 along with pGL4.45[luc2P/ISRE/Hygro] (firefly luciferase) and pGL4.74[hRluc/TK] (renilla luciferase). At 24 hpt, cells were treated with various concentrations of IFN-α. Relative levels of fold induction of the cell-associated firefly luciferase activity, compared to the corresponding untreated cell control levels, at 18 h post-IFN-α treatment were estimated using a dual-luciferase assay kit and were normalized with renilla luciferase expression levels. The data represent means ± SEM of results from triplicate sample experiments. aa, P ≤ 0.01 (compared to siCnt plus IFN-α). (C to F) ISG mRNA levels in siRNA-transfected and IFN-α-treated samples were measured for Mx1 (C), OAS1 (D), PKR (E), and ISG15 (F) using Sybr green qPCR. Fold change in mRNA levels compared to the untransfected control was calculated using the 2 −ΔΔ CT method, and the RPS18 gene was used as the housekeeping gene. The data represent means ± SEM of results from three independent experiments. a, P ≤ 0.05; aa, P ≤ 0.01; aaa, P ≤ 0.001 (compared to siCnt plus IFN-α).

    Techniques Used: Transfection, Western Blot, Software, Activity Assay, Luciferase, Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction

    2) Product Images from "A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions ▿ That Have Dissimilar Geographic Distributions ▿ †"

    Article Title: A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions ▿ That Have Dissimilar Geographic Distributions ▿ †

    Journal:

    doi: 10.1128/JB.01264-07

    Multiplex SYBR green real-time PCR assay targeting genes btfc-orf18 (the BTFC gene cluster target) and BPSS0120 (the YLF gene cluster target). This assay divides B. pseudomallei into two distinct groups. Strain 305 was the positive control strain for
    Figure Legend Snippet: Multiplex SYBR green real-time PCR assay targeting genes btfc-orf18 (the BTFC gene cluster target) and BPSS0120 (the YLF gene cluster target). This assay divides B. pseudomallei into two distinct groups. Strain 305 was the positive control strain for

    Techniques Used: Multiplex Assay, SYBR Green Assay, Real-time Polymerase Chain Reaction, Positive Control

    3) Product Images from "Using Advanced Intercross Lines for High-Resolution Mapping of HDL Cholesterol Quantitative Trait Loci"

    Article Title: Using Advanced Intercross Lines for High-Resolution Mapping of HDL Cholesterol Quantitative Trait Loci

    Journal: Genome Research

    doi: 10.1101/gr.1185803

    Real-time PCR quantification of mRNA expression levels of candidate genes for plasma HDL concentrations in B6 and NZB mice. Total RNA was extracted from the livers of female mice-fed chow and from mice fed a high-fat diet for 4 weeks. It was then transcribed into cDNA. mRNA expression levels for each candidate gene was quantified with real time PCR by using fluorescent SYBR. Results were normalized to Gapd and expressed as mRNA copies of candidates per 1000 copies of Gapd . ( * P
    Figure Legend Snippet: Real-time PCR quantification of mRNA expression levels of candidate genes for plasma HDL concentrations in B6 and NZB mice. Total RNA was extracted from the livers of female mice-fed chow and from mice fed a high-fat diet for 4 weeks. It was then transcribed into cDNA. mRNA expression levels for each candidate gene was quantified with real time PCR by using fluorescent SYBR. Results were normalized to Gapd and expressed as mRNA copies of candidates per 1000 copies of Gapd . ( * P

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Mouse Assay

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Disruption of the Zdhhc9 intellectual disability gene leads to behavioural abnormalities in a mouse model
    Article Snippet: .. Quantitative PCR (RT-qPCR) was performed in an Applied Biosystems StepOnePlus Real-Time PCR System, using the SYBR Select Master Mix (Life Technologies). .. The PCR reaction consisted of an initial polymerase activation step of 2 min at 95 °C, followed by 40 cycles of a denaturing stage of 15 s at 95 °C and an annealing and extension step at 60 °C for 1 min. A post-PCR melt curve analysis was included for qualitative analyses of the PCR product.

    Article Title: Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1)
    Article Snippet: .. Quantitative real time PCR (qRT-PCR) was performed on a ViiA7 with SYBR Select Master Mix (Life Technologies). .. Relative mRNA expression was calculated by the comparative ΔΔCt-Method normalized to the housekeeping gene ribosomal protein, large, P0 (RPLP0).

    Article Title: The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection
    Article Snippet: .. SYBR Select master mix (Life Technologies) was used, and RT-qPCR was performed using a CFX96 Touch real-time PCR detection system. .. Oligonucleotides were as follows: β-actin, Fw, AGT GTG ACG TTG ACA TCC GT, and Rv, TGC TAG GAG CCA GAG CAG TA; Isg15, Fw, TGA GCA TCC TGG TGA GGA ACG AAA, and Rv, AGC CAG AAC TGG TCT TCG TGA CTT; Vps4A, Fw, GAC AAC GTC AAC CCT CCA GA, and Rv, AGC ATG CTG GTA GAG ACG GA; Vps4B, Fw, GCC TTG TCT GTA GTA GGG GAC, and Rv, TTC CCA GCT TTG TCT TCC TGG; Chmp4B, Fw, GCC CGA AAC AGT CCC TCT AC, and Rv, TTC CTT CTT CTT GGC GGG TT; Chmp2B, Fw, AAG CAG CTT GTC CAC CTA CG, and Rv, TTG CAT TGT CTT TGC AGT GGT.

    Step One RT-PCR:

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. 4.8. qRT-PCR RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Disruption of the Zdhhc9 intellectual disability gene leads to behavioural abnormalities in a mouse model
    Article Snippet: .. Quantitative PCR (RT-qPCR) was performed in an Applied Biosystems StepOnePlus Real-Time PCR System, using the SYBR Select Master Mix (Life Technologies). .. The PCR reaction consisted of an initial polymerase activation step of 2 min at 95 °C, followed by 40 cycles of a denaturing stage of 15 s at 95 °C and an annealing and extension step at 60 °C for 1 min. A post-PCR melt curve analysis was included for qualitative analyses of the PCR product.

    Article Title: Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1)
    Article Snippet: .. Quantitative real time PCR (qRT-PCR) was performed on a ViiA7 with SYBR Select Master Mix (Life Technologies). .. Relative mRNA expression was calculated by the comparative ΔΔCt-Method normalized to the housekeeping gene ribosomal protein, large, P0 (RPLP0).

    Article Title: The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection
    Article Snippet: .. SYBR Select master mix (Life Technologies) was used, and RT-qPCR was performed using a CFX96 Touch real-time PCR detection system. .. Oligonucleotides were as follows: β-actin, Fw, AGT GTG ACG TTG ACA TCC GT, and Rv, TGC TAG GAG CCA GAG CAG TA; Isg15, Fw, TGA GCA TCC TGG TGA GGA ACG AAA, and Rv, AGC CAG AAC TGG TCT TCG TGA CTT; Vps4A, Fw, GAC AAC GTC AAC CCT CCA GA, and Rv, AGC ATG CTG GTA GAG ACG GA; Vps4B, Fw, GCC TTG TCT GTA GTA GGG GAC, and Rv, TTC CCA GCT TTG TCT TCC TGG; Chmp4B, Fw, GCC CGA AAC AGT CCC TCT AC, and Rv, TTC CTT CTT CTT GGC GGG TT; Chmp2B, Fw, AAG CAG CTT GTC CAC CTA CG, and Rv, TTG CAT TGT CTT TGC AGT GGT.

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. 4.8. qRT-PCR RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    SYBR Green Assay:

    Article Title: A Transcriptionally Permissive Epigenetic Landscape at the Vasoactive Intestinal Peptide Receptor-1 Promoter Suggests an Euchromatin Nuclear Position in Murine CD4 T Cells
    Article Snippet: .. SYBR green master mix and Taqman master mix were obtained from Applied Biosystems. .. The following antibodies (and clones) were obtained from Biolegend: anti-CD4-FITC (RM4-4), anti-CD4-FITC-PE-Cy5 (G1C1.5), anti-CD25-PE (3C7), anti-CD43-PE (1B1), anti-CD44-PE (IM7), anti-CD127-PE (1L-7Ra), anti-CD62L-PE (Mel-14), anti-CD117-PE (ACK2), anti-CD44-FITC (IM7), antiCD-69-PE (H1,2F3) and antiCD45R-FITC (RA3-6B2).

    Article Title: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
    Article Snippet: .. Performance of three different PCR mixes was compared, including SYBR Select Master Mix (Applied Biosystems), iQ SYBR green supermix (Bio-Rad, Hercules, CA, USA), and FastStart essential DNA Green Master (Roche Diagnostics). .. We evaluated four different HRM mixes on the lighter cycler 96, namely high resolution melting master (Roche Diagnostics), SensiFast HRM Kit (Bioline, London, UK), qPCRBIO HRM Mix(PCR Biosystems, London, UK), and MeltDoctor HRM Master Mix (Applied Biosystems).

    Expressing:

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. 4.8. qRT-PCR RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles
    Article Snippet: .. RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
    Article Snippet: .. Performance of three different PCR mixes was compared, including SYBR Select Master Mix (Applied Biosystems), iQ SYBR green supermix (Bio-Rad, Hercules, CA, USA), and FastStart essential DNA Green Master (Roche Diagnostics). .. We evaluated four different HRM mixes on the lighter cycler 96, namely high resolution melting master (Roche Diagnostics), SensiFast HRM Kit (Bioline, London, UK), qPCRBIO HRM Mix(PCR Biosystems, London, UK), and MeltDoctor HRM Master Mix (Applied Biosystems).

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  • 90
    Thermo Fisher sybr green i
    Correlation of detection of virus by Bio-Plex and RT-qPCR. Correlation between qPCR (Ct value) and Bio-Plex (MFI) detection of in-vitro grown ISAV standard curve by (A) <t>SYBR</t> <t>GREEN</t> I and (B)TaqMan; Correlation of detection of copy number of ISAV in plasma samples by (C) SYBR Green I (n = 13) and (D) TaqMan chemistries (n = 8).
    Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i/product/Thermo Fisher
    Average 90 stars, based on 13018 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2020-08
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    99
    Thermo Fisher fast sybr green master mix
    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with <t>SYBR</t> ™ Safe DNA Gel Stain in agarose gels. (C) <t>qRT-PCR</t> was performed to confirm the results in (B).
    Fast Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybr green master mix/product/Thermo Fisher
    Average 99 stars, based on 3358 article reviews
    Price from $9.99 to $1999.99
    fast sybr green master mix - by Bioz Stars, 2020-08
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    Thermo Fisher real time quantitative pcr analysis
    Confirmation of RNA and protein expression levels by real-time quantitative <t>PCR</t> and western blotting analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blotting analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t -test for independent samples. * p
    Real Time Quantitative Pcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative pcr analysis/product/Thermo Fisher
    Average 99 stars, based on 266 article reviews
    Price from $9.99 to $1999.99
    real time quantitative pcr analysis - by Bioz Stars, 2020-08
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    Image Search Results


    Correlation of detection of virus by Bio-Plex and RT-qPCR. Correlation between qPCR (Ct value) and Bio-Plex (MFI) detection of in-vitro grown ISAV standard curve by (A) SYBR GREEN I and (B)TaqMan; Correlation of detection of copy number of ISAV in plasma samples by (C) SYBR Green I (n = 13) and (D) TaqMan chemistries (n = 8).

    Journal: PLoS ONE

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology

    doi: 10.1371/journal.pone.0159155

    Figure Lengend Snippet: Correlation of detection of virus by Bio-Plex and RT-qPCR. Correlation between qPCR (Ct value) and Bio-Plex (MFI) detection of in-vitro grown ISAV standard curve by (A) SYBR GREEN I and (B)TaqMan; Correlation of detection of copy number of ISAV in plasma samples by (C) SYBR Green I (n = 13) and (D) TaqMan chemistries (n = 8).

    Article Snippet: 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix.

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction, In Vitro, SYBR Green Assay

    RT-qPCR standard curves. Standard curve relating viral copy number to Ct value from RT-Polymerase chain reaction using 10-fold dilutions of in-vitro transcribed RNA using (a) SYBR ® Green I and (b) TaqMan chemistries.

    Journal: PLoS ONE

    Article Title: Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology

    doi: 10.1371/journal.pone.0159155

    Figure Lengend Snippet: RT-qPCR standard curves. Standard curve relating viral copy number to Ct value from RT-Polymerase chain reaction using 10-fold dilutions of in-vitro transcribed RNA using (a) SYBR ® Green I and (b) TaqMan chemistries.

    Article Snippet: 2.3.1 qRT-PCR with SYBR Green I chemistry or TaqMan® chemistry Real time PCR was performed on first strand cDNA (reverse transcribed from in-vitro transcribed RNA) using the Eppendorf® RealPlex2 Mastercycler gradient S instrument, using ISAV Segment 8 primer pair ISAV8F/ISAV8R (amplifying a 104 bp product), [ ], either with SYBR® Green I (Thermo Scientific) master mix or with TaqMan® master mix.

    Techniques: Quantitative RT-PCR, Polymerase Chain Reaction, In Vitro, SYBR Green Assay

    Silencing of Snail in Caki-2 by a lentivirus-delivered short inhibitory RNA. A. Map of the lentivirualRNAi vectors [pCMV-G NR-U6-shRNA]. U6, polymerase III promoter to drive the transcription of shRNAs; GFP (green fluorescent protein) were driven by CMV promoter. shRNAs were inserted into HindIII and BamHI sites. B. Strategy plan for generating Snail shRNA constructs. pCMV-G NR-U6 vector was linearized by HindIII and BamH1, and then designed shRNAs were inserted into this site. The positive colonies were screened by colony PCR using the primers flanking the inserted site of shRNA. Selected Colonies were finally confirmed by sequencing. C. Quantitative real-time RT-PCR compare the knock-down efficiency of 3 Snail shRNAs in Caki-2 cells. RNAs were isolated from cells and gene reverse transcription was performed. Quantitative PCR was performed by using SYBR Green PCR Master Mix. Data were normalized to GAPDH mRNA levels, by using efficiency (2 -ΔΔCt ) method. The data were analyzed by student t -test, and were presented as the means ± s.e.m (n = 3). D. Western blotting assay confirmed that effectiveness of Snail shRNA viruses on protein level.The intensity of bands were determined by Image J, and ratio of Snail to GAPDH were shown here.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways

    doi:

    Figure Lengend Snippet: Silencing of Snail in Caki-2 by a lentivirus-delivered short inhibitory RNA. A. Map of the lentivirualRNAi vectors [pCMV-G NR-U6-shRNA]. U6, polymerase III promoter to drive the transcription of shRNAs; GFP (green fluorescent protein) were driven by CMV promoter. shRNAs were inserted into HindIII and BamHI sites. B. Strategy plan for generating Snail shRNA constructs. pCMV-G NR-U6 vector was linearized by HindIII and BamH1, and then designed shRNAs were inserted into this site. The positive colonies were screened by colony PCR using the primers flanking the inserted site of shRNA. Selected Colonies were finally confirmed by sequencing. C. Quantitative real-time RT-PCR compare the knock-down efficiency of 3 Snail shRNAs in Caki-2 cells. RNAs were isolated from cells and gene reverse transcription was performed. Quantitative PCR was performed by using SYBR Green PCR Master Mix. Data were normalized to GAPDH mRNA levels, by using efficiency (2 -ΔΔCt ) method. The data were analyzed by student t -test, and were presented as the means ± s.e.m (n = 3). D. Western blotting assay confirmed that effectiveness of Snail shRNA viruses on protein level.The intensity of bands were determined by Image J, and ratio of Snail to GAPDH were shown here.

    Article Snippet: Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System.

    Techniques: shRNA, Construct, Plasmid Preparation, Polymerase Chain Reaction, Sequencing, Quantitative RT-PCR, Isolation, Real-time Polymerase Chain Reaction, SYBR Green Assay, Western Blot

    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Journal: Heart rhythm

    Article Title: HuR-mediated SCN5A mRNA stability reduces arrhythmic risk in heart failure

    doi: 10.1016/j.hrthm.2018.02.018

    Figure Lengend Snippet: HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Article Snippet: Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out using gene-specific primers, Fast SYBR® Green Master Mix and 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Isolation, Synthesized, Polymerase Chain Reaction, Staining, Quantitative RT-PCR

    Confirmation of RNA and protein expression levels by real-time quantitative PCR and western blotting analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blotting analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t -test for independent samples. * p

    Journal: Proteome Science

    Article Title: Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells

    doi: 10.1186/1477-5956-12-13

    Figure Lengend Snippet: Confirmation of RNA and protein expression levels by real-time quantitative PCR and western blotting analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blotting analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t -test for independent samples. * p

    Article Snippet: The cDNA was then used as the template for real-time quantitative PCR analysis (Maxima SYBR Green/ROX qPCR Master Mix; Thermo Scientific, Pittsburgh, PA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot