sybr green pcr master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sybr green pcr master mix
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 8949 article reviews
    Price from $9.99 to $1999.99
    sybr green pcr master mix - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

    Amplification:

    Article Title: Macrophage Migration Inhibitory Factor Is a Molecular Determinant of the Anti-EGFR Monoclonal Antibody Cetuximab Resistance in Human Colorectal Cancer Cells
    Article Snippet: .. RNA Extraction and qRT-PCR Total RNA was prepared using TRIsure reagent (BioLine, Randolph, MA, USA) and reverse-transcribed into cDNA by SensiFast reverse transcriptase (BioLine), according to the manufacturer’s instructions. qRT-PCR analysis was performed using the following primers: MIF Fw: 5′-CCCGGACAGGGTCTACATCA-3′; MIF Rv: 5′-GGAGTTGTTCCAGCCCACAT-3′; 18S Fw: 5′-GGCGACGACCCATTCGAAC-3′; 18S Rv: 5′-AGGCACGGCGACTACCATC-3′; Amplification was conducted using the SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicate using a Quant studio 7 Flex (Applied Biosystem).

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: .. For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids. .. We used 10 μ l of the real-time SYBR Green PCR Master Mix (2x), 2 μ l of the cDNA sample at a concentration of 0.2 μ g/μ l, 2 μ l of a first sense primer, 2 μ l of a reverse primer, and 4 μ l of ultrapure water.

    Article Title: CIRP-derived Peptide C23 Attenuates Inflammation and Tissue Injury in a Murine Model of Intestinal Ischemia-Reperfusion
    Article Snippet: A quantitative real-time PCR assay was carried-out in 20 μl of a final volume containing 0.08 μmol of each forward and reverse primer, cDNA (2 μl of 10× diluted cDNA), and 10 μl SYBR Green PCR master mix (Applied Biosystems). .. Amplification was conducted in an Applied Biosystems Step One Plus real-time PCR machine.

    Article Title: A Novel Microbial Culture Chamber Co-cultivation System to Study Algal-Bacteria Interactions Using Emiliania huxleyi and Phaeobacter inhibens as Model Organisms
    Article Snippet: qPCR Procedures qPCR amplifications were performed on 1–10 μl template DNA (10 ng) using SYBR Green PCR Master Mix (Applied Biosystems, CA, United States, Cat.No. .. The PCR amplification (10 min at 95°C followed by 40 cycles of three steps consisting of 15 s at 95°C, 1 min at 58°C, and 1 min at 72°C) was performed with an Mx3005P qPCR thermal cycler (Stratagene, La Jolla, CA, United States).

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: Standard curves were performed in order to obtain the concentration and amplification efficiency of the primers to be used. .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer.

    Synthesized:

    Article Title: The molecular landscape of glioma in patients with Neurofibromatosis 1
    Article Snippet: Total RNA was prepared using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturer’s instructions, and cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen). .. RT-qPCR was performed with a 7500 Real Time PCR thermal cycler system (Applied Biosystems), using SYBR Green PCR Master Mix (Applied Biosystems).

    Article Title: C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo
    Article Snippet: RNA was extracted with an RNeasy kit (Qiagen), and complementary DNA was synthesized with random hexamers and TaqMan reverse transcriptase (Applied Biosystems). .. For quantitative PCR, these primers were used in reactions with SYBR Green PCR master mix (Applied Biosystems).

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: RT-qPCRs were performed using the Applied Biosystems 7300 Real-Time PCR system equipment and SYBR green to detect the synthesized dsDNAs. .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer.

    Quantitative RT-PCR:

    Article Title: The molecular landscape of glioma in patients with Neurofibromatosis 1
    Article Snippet: .. RT-qPCR was performed with a 7500 Real Time PCR thermal cycler system (Applied Biosystems), using SYBR Green PCR Master Mix (Applied Biosystems). ..

    Article Title: Macrophage Migration Inhibitory Factor Is a Molecular Determinant of the Anti-EGFR Monoclonal Antibody Cetuximab Resistance in Human Colorectal Cancer Cells
    Article Snippet: .. RNA Extraction and qRT-PCR Total RNA was prepared using TRIsure reagent (BioLine, Randolph, MA, USA) and reverse-transcribed into cDNA by SensiFast reverse transcriptase (BioLine), according to the manufacturer’s instructions. qRT-PCR analysis was performed using the following primers: MIF Fw: 5′-CCCGGACAGGGTCTACATCA-3′; MIF Rv: 5′-GGAGTTGTTCCAGCCCACAT-3′; 18S Fw: 5′-GGCGACGACCCATTCGAAC-3′; 18S Rv: 5′-AGGCACGGCGACTACCATC-3′; Amplification was conducted using the SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicate using a Quant studio 7 Flex (Applied Biosystem).

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: .. For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids. .. We used 10 μ l of the real-time SYBR Green PCR Master Mix (2x), 2 μ l of the cDNA sample at a concentration of 0.2 μ g/μ l, 2 μ l of a first sense primer, 2 μ l of a reverse primer, and 4 μ l of ultrapure water.

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: Paragraph title: RNA Extraction And Quantitative Real-Time PCR (qRT-PCR) ... Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System.

    Article Title: Dapagliflozin Attenuates Hyperglycemia Related Osteoporosis in ZDF Rats by Alleviating Hypercalciuria
    Article Snippet: Paragraph title: Quantitative RT-PCR Assay ... The reaction mixtures were prepared using SYBR® Green PCR Master Mix (Life technology, Carlsbad, CA 92008 USA), 0.5 μM of each primer, 4 mM MgCl2 and 2 μl of cDNA in a final volume of 20 μl.

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems). .. Fold change was determined by comparing target gene expression with the reference gene Tbp , which was chosen because its levels do not vary significantly across adipocyte types and temperatures, unlike other commonly used references genes, such as 36B4 ( Rplp0 ), Rps18, Gapdh, cyclophilin A (Ppia) and β-actin ( Actb ).

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer. ..

    SYBR Green Assay:

    Article Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein
    Article Snippet: .. E2F and DP antibodies were gifts from Dr. Nicholas Dyson (Harvard Medical School). qPCR using SYBR green PCR Master Mix (Applied Biosystems) was performed on two biological replicates, and results in show averages of these experiments. .. Sequences for primers used for PCR were situated under predicted Rbf1 binding areas in promoter proximal regions, except for negative control genes, and are available upon request.

    Article Title: The molecular landscape of glioma in patients with Neurofibromatosis 1
    Article Snippet: .. RT-qPCR was performed with a 7500 Real Time PCR thermal cycler system (Applied Biosystems), using SYBR Green PCR Master Mix (Applied Biosystems). ..

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: .. SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California). .. Relative mRNA expression was calculated employing the ΔΔCt method using β-actin as the housekeeping gene and the vehicle-air treated SH group as control.

    Article Title: Macrophage Migration Inhibitory Factor Is a Molecular Determinant of the Anti-EGFR Monoclonal Antibody Cetuximab Resistance in Human Colorectal Cancer Cells
    Article Snippet: .. RNA Extraction and qRT-PCR Total RNA was prepared using TRIsure reagent (BioLine, Randolph, MA, USA) and reverse-transcribed into cDNA by SensiFast reverse transcriptase (BioLine), according to the manufacturer’s instructions. qRT-PCR analysis was performed using the following primers: MIF Fw: 5′-CCCGGACAGGGTCTACATCA-3′; MIF Rv: 5′-GGAGTTGTTCCAGCCCACAT-3′; 18S Fw: 5′-GGCGACGACCCATTCGAAC-3′; 18S Rv: 5′-AGGCACGGCGACTACCATC-3′; Amplification was conducted using the SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicate using a Quant studio 7 Flex (Applied Biosystem).

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: .. For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids. .. We used 10 μ l of the real-time SYBR Green PCR Master Mix (2x), 2 μ l of the cDNA sample at a concentration of 0.2 μ g/μ l, 2 μ l of a first sense primer, 2 μ l of a reverse primer, and 4 μ l of ultrapure water.

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: .. Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System. .. The miRNA level was determined using qRT-PCR analysis with TaqMan MicroRNA Assay Kit (Applied Biosystems).

    Article Title: C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo
    Article Snippet: .. For quantitative PCR, these primers were used in reactions with SYBR Green PCR master mix (Applied Biosystems). .. For quantitative PCR, primers specific for Flag-(GR) 80 are: forward primer 5′-GGCGCTTTCTCATAG CTCAC-3′ and reverse primer 5′-GCCTACATACCTCGCTCTGC-3′.

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Relative expression level was analyzed by the 2−△△Ct method, normalized to expression of GAPDH, and presented as mean ± SD of replicates.

    Article Title: Dapagliflozin Attenuates Hyperglycemia Related Osteoporosis in ZDF Rats by Alleviating Hypercalciuria
    Article Snippet: .. The reaction mixtures were prepared using SYBR® Green PCR Master Mix (Life technology, Carlsbad, CA 92008 USA), 0.5 μM of each primer, 4 mM MgCl2 and 2 μl of cDNA in a final volume of 20 μl. ..

    Article Title: CIRP-derived Peptide C23 Attenuates Inflammation and Tissue Injury in a Murine Model of Intestinal Ischemia-Reperfusion
    Article Snippet: .. A quantitative real-time PCR assay was carried-out in 20 μl of a final volume containing 0.08 μmol of each forward and reverse primer, cDNA (2 μl of 10× diluted cDNA), and 10 μl SYBR Green PCR master mix (Applied Biosystems). .. Amplification was conducted in an Applied Biosystems Step One Plus real-time PCR machine.

    Article Title: A Novel Microbial Culture Chamber Co-cultivation System to Study Algal-Bacteria Interactions Using Emiliania huxleyi and Phaeobacter inhibens as Model Organisms
    Article Snippet: .. qPCR Procedures qPCR amplifications were performed on 1–10 μl template DNA (10 ng) using SYBR Green PCR Master Mix (Applied Biosystems, CA, United States, Cat.No. ..

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems). .. Fold change was determined by comparing target gene expression with the reference gene Tbp , which was chosen because its levels do not vary significantly across adipocyte types and temperatures, unlike other commonly used references genes, such as 36B4 ( Rplp0 ), Rps18, Gapdh, cyclophilin A (Ppia) and β-actin ( Actb ).

    Article Title: In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
    Article Snippet: .. Each 25 µL reaction contained 12.5 µl SYBR Green PCR Master Mix (Thermo Fisher Scientific), 250 nM of each primer, and 1ul cDNA. .. All reactions were carried out in triplicate on an AB7500 Real-Time PCR system (Applied Biosystems).

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer. ..

    Incubation:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: For viral inoculation, SCC15, SCC25, or CAL27 cells were incubated with the viral supernatants (MOI = 10) in complete medium for 24 h. Medium was switched to standard culture media 24 h post infection. .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems).

    Expressing:

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California). .. Relative mRNA expression was calculated employing the ΔΔCt method using β-actin as the housekeeping gene and the vehicle-air treated SH group as control.

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: The analysis of the gene expression used in our study represents the relative quantification of the genes of interest using an endogenous control (normalizing gene). .. For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids.

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Relative expression level was analyzed by the 2−△△Ct method, normalized to expression of GAPDH, and presented as mean ± SD of replicates.

    Article Title: Dapagliflozin Attenuates Hyperglycemia Related Osteoporosis in ZDF Rats by Alleviating Hypercalciuria
    Article Snippet: To quantify disease or drug-induced changes in osteal expression of genes involved in calcium and phosphate regulation, quantitative RT-PCR was performed. .. The reaction mixtures were prepared using SYBR® Green PCR Master Mix (Life technology, Carlsbad, CA 92008 USA), 0.5 μM of each primer, 4 mM MgCl2 and 2 μl of cDNA in a final volume of 20 μl.

    Article Title: CIRP-derived Peptide C23 Attenuates Inflammation and Tissue Injury in a Murine Model of Intestinal Ischemia-Reperfusion
    Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction (PCR) for determining the expression of IL-6 and MIP-2 mRNAs in lung ... A quantitative real-time PCR assay was carried-out in 20 μl of a final volume containing 0.08 μmol of each forward and reverse primer, cDNA (2 μl of 10× diluted cDNA), and 10 μl SYBR Green PCR master mix (Applied Biosystems).

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems). .. Fold change was determined by comparing target gene expression with the reference gene Tbp , which was chosen because its levels do not vary significantly across adipocyte types and temperatures, unlike other commonly used references genes, such as 36B4 ( Rplp0 ), Rps18, Gapdh, cyclophilin A (Ppia) and β-actin ( Actb ).

    Article Title: In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
    Article Snippet: Gene expression was quantified by quantitative PCR. .. Each 25 µL reaction contained 12.5 µl SYBR Green PCR Master Mix (Thermo Fisher Scientific), 250 nM of each primer, and 1ul cDNA.

    BIA-KA:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Western Blot Analysis : Cells were lysed in RIPA buffer with 1 mmol L−1 phenylmethylsulfonyl fluoride (PMSF), normalized by BCA protein kit, and degenerated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) loading buffer.

    Western Blot:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Western Blot Analysis : Cells were lysed in RIPA buffer with 1 mmol L−1 phenylmethylsulfonyl fluoride (PMSF), normalized by BCA protein kit, and degenerated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) loading buffer.

    Activated Clotting Time Assay:

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System. .. The primer sequences were listed as follows: miR-769-5p RT 5´-CTC AAC TGG TGT CGT GGA GTC GGC AAT TCA GTT GAG AAC CCA TG-3´, forward 5´-ACA CTC CAG CTG GGT GAG ACC TCT GGG TTC TG-3´ and reverse 5´-CTC AAC TGG TGT CGT GGA-3´; U6 forward 5ʹ-CTC GCT TCG GCA GCA CA-3ʹ and reverse 5ʹ-AAC GCT TCA CGA ATT TGC GT-3ʹ; KMT2A forward 5ʹ-CCC CTT CGG GTC TCC TCA TT −3ʹ and reverse 5ʹ-ATC CTT CGA GGG CTT TGT CTG-3ʹ; protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1) forward 5′-TGT CTC TGG AGG AGA GCT ATT TGA-3′ and reverse 5′-GGT GAG CCA CAG CTT GTT CTT-3′; GAPDH forward 5ʹ-TGT TCG TCA TGG GTG TGA AC-3ʹ and reverse 5ʹ-ATG GCA TGG ACT GTG GTC AT-3ʹ.

    Transfection:

    Article Title: In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
    Article Snippet: RNA extractions, cDNA synthesis and quantitative PCR RNA was extracted 24 hours after transfection with the RNeasy plus minikit (Qiagen) following manufacturer’s instructions. .. Each 25 µL reaction contained 12.5 µl SYBR Green PCR Master Mix (Thermo Fisher Scientific), 250 nM of each primer, and 1ul cDNA.

    Ligation:

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

    Infection:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: For viral inoculation, SCC15, SCC25, or CAL27 cells were incubated with the viral supernatants (MOI = 10) in complete medium for 24 h. Medium was switched to standard culture media 24 h post infection. .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems).

    TaqMan microRNA Assay:

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System. .. The miRNA level was determined using qRT-PCR analysis with TaqMan MicroRNA Assay Kit (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: Paragraph title: RNA isolation/Quantitative RT-PCR ... Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems).

    Sequencing:

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: .. SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California). .. Relative mRNA expression was calculated employing the ΔΔCt method using β-actin as the housekeeping gene and the vehicle-air treated SH group as control.

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: Detection of Gene Transcripts by q-PCR Messenger RNAs (mRNAs) were indirectly verified by real-time PCR cDNA amplification with specific primers to LIC13479 and LIC10050 (Lsa46 and Lsa77 respectively) coding sequence. .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer.

    Binding Assay:

    Article Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein
    Article Snippet: E2F and DP antibodies were gifts from Dr. Nicholas Dyson (Harvard Medical School). qPCR using SYBR green PCR Master Mix (Applied Biosystems) was performed on two biological replicates, and results in show averages of these experiments. .. Sequences for primers used for PCR were situated under predicted Rbf1 binding areas in promoter proximal regions, except for negative control genes, and are available upon request.

    Isolation:

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: Paragraph title: Lung RNA isolation and real time-quantitative PCR ... SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California).

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative Real‐Time PCR Analysis : RNA extraction was isolated from cells or tissues with TRIZOL (TAKARA) according to the manufacturer's protocol. .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems).

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: Paragraph title: RNA isolation/Quantitative RT-PCR ... Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems).

    Negative Control:

    Article Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein
    Article Snippet: E2F and DP antibodies were gifts from Dr. Nicholas Dyson (Harvard Medical School). qPCR using SYBR green PCR Master Mix (Applied Biosystems) was performed on two biological replicates, and results in show averages of these experiments. .. Sequences for primers used for PCR were situated under predicted Rbf1 binding areas in promoter proximal regions, except for negative control genes, and are available upon request.

    Purification:

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

    Polymerase Chain Reaction:

    Article Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein
    Article Snippet: .. E2F and DP antibodies were gifts from Dr. Nicholas Dyson (Harvard Medical School). qPCR using SYBR green PCR Master Mix (Applied Biosystems) was performed on two biological replicates, and results in show averages of these experiments. .. Sequences for primers used for PCR were situated under predicted Rbf1 binding areas in promoter proximal regions, except for negative control genes, and are available upon request.

    Article Title: The molecular landscape of glioma in patients with Neurofibromatosis 1
    Article Snippet: .. RT-qPCR was performed with a 7500 Real Time PCR thermal cycler system (Applied Biosystems), using SYBR Green PCR Master Mix (Applied Biosystems). ..

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: .. SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California). .. Relative mRNA expression was calculated employing the ΔΔCt method using β-actin as the housekeeping gene and the vehicle-air treated SH group as control.

    Article Title: Macrophage Migration Inhibitory Factor Is a Molecular Determinant of the Anti-EGFR Monoclonal Antibody Cetuximab Resistance in Human Colorectal Cancer Cells
    Article Snippet: .. RNA Extraction and qRT-PCR Total RNA was prepared using TRIsure reagent (BioLine, Randolph, MA, USA) and reverse-transcribed into cDNA by SensiFast reverse transcriptase (BioLine), according to the manufacturer’s instructions. qRT-PCR analysis was performed using the following primers: MIF Fw: 5′-CCCGGACAGGGTCTACATCA-3′; MIF Rv: 5′-GGAGTTGTTCCAGCCCACAT-3′; 18S Fw: 5′-GGCGACGACCCATTCGAAC-3′; 18S Rv: 5′-AGGCACGGCGACTACCATC-3′; Amplification was conducted using the SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicate using a Quant studio 7 Flex (Applied Biosystem).

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: .. For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids. .. We used 10 μ l of the real-time SYBR Green PCR Master Mix (2x), 2 μ l of the cDNA sample at a concentration of 0.2 μ g/μ l, 2 μ l of a first sense primer, 2 μ l of a reverse primer, and 4 μ l of ultrapure water.

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: .. Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System. .. The miRNA level was determined using qRT-PCR analysis with TaqMan MicroRNA Assay Kit (Applied Biosystems).

    Article Title: C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo
    Article Snippet: .. For quantitative PCR, these primers were used in reactions with SYBR Green PCR master mix (Applied Biosystems). .. For quantitative PCR, primers specific for Flag-(GR) 80 are: forward primer 5′-GGCGCTTTCTCATAG CTCAC-3′ and reverse primer 5′-GCCTACATACCTCGCTCTGC-3′.

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Relative expression level was analyzed by the 2−△△Ct method, normalized to expression of GAPDH, and presented as mean ± SD of replicates.

    Article Title: Dapagliflozin Attenuates Hyperglycemia Related Osteoporosis in ZDF Rats by Alleviating Hypercalciuria
    Article Snippet: .. The reaction mixtures were prepared using SYBR® Green PCR Master Mix (Life technology, Carlsbad, CA 92008 USA), 0.5 μM of each primer, 4 mM MgCl2 and 2 μl of cDNA in a final volume of 20 μl. ..

    Article Title: CIRP-derived Peptide C23 Attenuates Inflammation and Tissue Injury in a Murine Model of Intestinal Ischemia-Reperfusion
    Article Snippet: .. A quantitative real-time PCR assay was carried-out in 20 μl of a final volume containing 0.08 μmol of each forward and reverse primer, cDNA (2 μl of 10× diluted cDNA), and 10 μl SYBR Green PCR master mix (Applied Biosystems). .. Amplification was conducted in an Applied Biosystems Step One Plus real-time PCR machine.

    Article Title: A Novel Microbial Culture Chamber Co-cultivation System to Study Algal-Bacteria Interactions Using Emiliania huxleyi and Phaeobacter inhibens as Model Organisms
    Article Snippet: .. qPCR Procedures qPCR amplifications were performed on 1–10 μl template DNA (10 ng) using SYBR Green PCR Master Mix (Applied Biosystems, CA, United States, Cat.No. ..

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems). .. Fold change was determined by comparing target gene expression with the reference gene Tbp , which was chosen because its levels do not vary significantly across adipocyte types and temperatures, unlike other commonly used references genes, such as 36B4 ( Rplp0 ), Rps18, Gapdh, cyclophilin A (Ppia) and β-actin ( Actb ).

    Article Title: In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
    Article Snippet: .. Each 25 µL reaction contained 12.5 µl SYBR Green PCR Master Mix (Thermo Fisher Scientific), 250 nM of each primer, and 1ul cDNA. .. All reactions were carried out in triplicate on an AB7500 Real-Time PCR system (Applied Biosystems).

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Western Blot Analysis : Cells were lysed in RIPA buffer with 1 mmol L−1 phenylmethylsulfonyl fluoride (PMSF), normalized by BCA protein kit, and degenerated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) loading buffer.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: A Novel Microbial Culture Chamber Co-cultivation System to Study Algal-Bacteria Interactions Using Emiliania huxleyi and Phaeobacter inhibens as Model Organisms
    Article Snippet: .. qPCR Procedures qPCR amplifications were performed on 1–10 μl template DNA (10 ng) using SYBR Green PCR Master Mix (Applied Biosystems, CA, United States, Cat.No. ..

    Chromatin Immunoprecipitation:

    Article Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein
    Article Snippet: Paragraph title: Chromatin Immunoprecipitation ... E2F and DP antibodies were gifts from Dr. Nicholas Dyson (Harvard Medical School). qPCR using SYBR green PCR Master Mix (Applied Biosystems) was performed on two biological replicates, and results in show averages of these experiments.

    SDS Page:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Western Blot Analysis : Cells were lysed in RIPA buffer with 1 mmol L−1 phenylmethylsulfonyl fluoride (PMSF), normalized by BCA protein kit, and degenerated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) loading buffer.

    Plasmid Preparation:

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: The vector information is listed in the Key Resources Table. .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein
    Article Snippet: .. E2F and DP antibodies were gifts from Dr. Nicholas Dyson (Harvard Medical School). qPCR using SYBR green PCR Master Mix (Applied Biosystems) was performed on two biological replicates, and results in show averages of these experiments. .. Sequences for primers used for PCR were situated under predicted Rbf1 binding areas in promoter proximal regions, except for negative control genes, and are available upon request.

    Article Title: The molecular landscape of glioma in patients with Neurofibromatosis 1
    Article Snippet: .. RT-qPCR was performed with a 7500 Real Time PCR thermal cycler system (Applied Biosystems), using SYBR Green PCR Master Mix (Applied Biosystems). ..

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: .. SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California). .. Relative mRNA expression was calculated employing the ΔΔCt method using β-actin as the housekeeping gene and the vehicle-air treated SH group as control.

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: Paragraph title: 2.8. Quantitative Real-Time PCR Analysis ... For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids.

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: .. Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System. .. The miRNA level was determined using qRT-PCR analysis with TaqMan MicroRNA Assay Kit (Applied Biosystems).

    Article Title: C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo
    Article Snippet: .. For quantitative PCR, these primers were used in reactions with SYBR Green PCR master mix (Applied Biosystems). .. For quantitative PCR, primers specific for Flag-(GR) 80 are: forward primer 5′-GGCGCTTTCTCATAG CTCAC-3′ and reverse primer 5′-GCCTACATACCTCGCTCTGC-3′.

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). .. Relative expression level was analyzed by the 2−△△Ct method, normalized to expression of GAPDH, and presented as mean ± SD of replicates.

    Article Title: Dapagliflozin Attenuates Hyperglycemia Related Osteoporosis in ZDF Rats by Alleviating Hypercalciuria
    Article Snippet: Real-time PCR was performed with the Applied Biosystems® 7500 Real-Time PCR Systems (Life technology, Carlsbad, CA 92008 USA). .. The reaction mixtures were prepared using SYBR® Green PCR Master Mix (Life technology, Carlsbad, CA 92008 USA), 0.5 μM of each primer, 4 mM MgCl2 and 2 μl of cDNA in a final volume of 20 μl.

    Article Title: CIRP-derived Peptide C23 Attenuates Inflammation and Tissue Injury in a Murine Model of Intestinal Ischemia-Reperfusion
    Article Snippet: .. A quantitative real-time PCR assay was carried-out in 20 μl of a final volume containing 0.08 μmol of each forward and reverse primer, cDNA (2 μl of 10× diluted cDNA), and 10 μl SYBR Green PCR master mix (Applied Biosystems). .. Amplification was conducted in an Applied Biosystems Step One Plus real-time PCR machine.

    Article Title: A Novel Microbial Culture Chamber Co-cultivation System to Study Algal-Bacteria Interactions Using Emiliania huxleyi and Phaeobacter inhibens as Model Organisms
    Article Snippet: .. qPCR Procedures qPCR amplifications were performed on 1–10 μl template DNA (10 ng) using SYBR Green PCR Master Mix (Applied Biosystems, CA, United States, Cat.No. ..

    Article Title: In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
    Article Snippet: Paragraph title: RNA extractions, cDNA synthesis and quantitative PCR ... Each 25 µL reaction contained 12.5 µl SYBR Green PCR Master Mix (Thermo Fisher Scientific), 250 nM of each primer, and 1ul cDNA.

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: RT-qPCRs were performed using the Applied Biosystems 7300 Real-Time PCR system equipment and SYBR green to detect the synthesized dsDNAs. .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer.

    RNA Extraction:

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: Anatomically uniform portions of frozen caudal lung lobes were separated and weighed (20–30 mg) for RNA extraction using the RNeasy mini kit (Qiagen, Valencia, California). .. SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California).

    Article Title: Macrophage Migration Inhibitory Factor Is a Molecular Determinant of the Anti-EGFR Monoclonal Antibody Cetuximab Resistance in Human Colorectal Cancer Cells
    Article Snippet: .. RNA Extraction and qRT-PCR Total RNA was prepared using TRIsure reagent (BioLine, Randolph, MA, USA) and reverse-transcribed into cDNA by SensiFast reverse transcriptase (BioLine), according to the manufacturer’s instructions. qRT-PCR analysis was performed using the following primers: MIF Fw: 5′-CCCGGACAGGGTCTACATCA-3′; MIF Rv: 5′-GGAGTTGTTCCAGCCCACAT-3′; 18S Fw: 5′-GGCGACGACCCATTCGAAC-3′; 18S Rv: 5′-AGGCACGGCGACTACCATC-3′; Amplification was conducted using the SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicate using a Quant studio 7 Flex (Applied Biosystem).

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: Paragraph title: RNA Extraction And Quantitative Real-Time PCR (qRT-PCR) ... Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System.

    Article Title: Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC, Periodic Oxaliplatin Administration in Synergy with PER2‐Mediated PCNA Transcription Repression Promotes Chronochemotherapeutic Efficacy of OSCC
    Article Snippet: Quantitative Real‐Time PCR Analysis : RNA extraction was isolated from cells or tissues with TRIZOL (TAKARA) according to the manufacturer's protocol. .. Quantitative real‐time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems).

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: We used TRIzol (Invitrogen) and Qiagen Micro RNeasy kit for total tissue RNA and TRAP-isolated RNA extraction, respectively, according to the manufacturers’ instructions. .. Quantitative RT-PCR (qRT-PCR) was performed using an Applied Biosystems 7900 thermocycler and SYBR Green PCR Master Mix (Applied Biosystems).

    RNA Expression:

    Article Title: Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats
    Article Snippet: SYBR® Green PCR Master Mix (Thermo Fisher, Waltham, Massachusetts) was used to perform quantitative PCR using the Applied Biosystems 7900HT Sequence Detection System (Foster City, California). .. The determination of relative lung mRNA expression was restricted to D + 1 groups since ozone-induced changes in RNA expression peak rapidly after first ozone exposure ( ; ).

    Spectrophotometry:

    Article Title: In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
    Article Snippet: RNA quantity and quality were evaluated with a Nanodrop Spectrophotometer (Labtech). cDNA synthesis was carried out with Superscript Vilo kit (Thermo Fisher Scientific). .. Each 25 µL reaction contained 12.5 µl SYBR Green PCR Master Mix (Thermo Fisher Scientific), 250 nM of each primer, and 1ul cDNA.

    Concentration Assay:

    Article Title: Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
    Article Snippet: For qRT-PCR, we used SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) for the amplification and quantification of nucleic acids. .. We used 10 μ l of the real-time SYBR Green PCR Master Mix (2x), 2 μ l of the cDNA sample at a concentration of 0.2 μ g/μ l, 2 μ l of a first sense primer, 2 μ l of a reverse primer, and 4 μ l of ultrapure water.

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: Standard curves were performed in order to obtain the concentration and amplification efficiency of the primers to be used. .. RT-qPCR was performed in a reaction volume of 25 μ l containing 25 ng of cDNA template, 300 nM each oligonucleotide (‘F' and ‘R'), and 12.5 μ l SYBR Green PCR Master Mix (Applied Biosystems) as recommended by the manufacturer.

    CTG Assay:

    Article Title: MicroRNA-769-5p Promotes The Growth Of Glioma Cells By Targeting Lysine Methyltransferase 2A
    Article Snippet: Real-time PCR assay was performed with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) in the ABI 7500 Real-time PCR System. .. The primer sequences were listed as follows: miR-769-5p RT 5´-CTC AAC TGG TGT CGT GGA GTC GGC AAT TCA GTT GAG AAC CCA TG-3´, forward 5´-ACA CTC CAG CTG GGT GAG ACC TCT GGG TTC TG-3´ and reverse 5´-CTC AAC TGG TGT CGT GGA-3´; U6 forward 5ʹ-CTC GCT TCG GCA GCA CA-3ʹ and reverse 5ʹ-AAC GCT TCA CGA ATT TGC GT-3ʹ; KMT2A forward 5ʹ-CCC CTT CGG GTC TCC TCA TT −3ʹ and reverse 5ʹ-ATC CTT CGA GGG CTT TGT CTG-3ʹ; protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1) forward 5′-TGT CTC TGG AGG AGA GCT ATT TGA-3′ and reverse 5′-GGT GAG CCA CAG CTT GTT CTT-3′; GAPDH forward 5ʹ-TGT TCG TCA TGG GTG TGA AC-3ʹ and reverse 5ʹ-ATG GCA TGG ACT GTG GTC AT-3ʹ.

    Gel Extraction:

    Article Title: RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Article Snippet: .. Ac-BSA (Sigma, cat. no. B8894) Acidic Tyrode’s solution (Sigma, cat. no. T1788) 1× PBS (pH 7.2) (Gibco, cat. no. 14249-95) 10× PCR Buffer II and 25 mM MgCl2 (Applied Biosystems, cat. no. 4379878) Nonidet P-40 SP (Roche, cat. no. 11332473001) SuperScript III Reverse Transcriptase with 0.1 M DTT (Invitrogen, cat. no. 18080-044 or 18080-085) RNase Inhibitor (Cloned) (40 U μl−1 ) (Applied Biosystems, cat. no. AM2682) SUPERase-In™ (20 U μl−1 ) (Applied Biosystems, cat. no. AM2694) T4 gene 32 Protein (Roche, cat. no. 972983) Exonuclease I (New England Biolabs, cat. no. M0293S) Terminal Transferase (TdT) (Invitrogen, cat. no. 10533-065 or 10533-073) 100 mM dATP (Promega, cat. no. U1201) Nuclease-free Water, 1 L (Applied Biosystems, cat. no. AM9932) RNase H (Invitrogen, cat. no. 18021-014 or 18021-071) TaKaRa Ex Taq™ HS (Includes: 10× ExTaq Buffer (mg2+ plus) and dNTP mixture) (Takara Bio Inc, cat. no. RR006A or RR006B) QIAquick PCR Purification Kit (250) (Qiagen, cat. no. 28106) QIAquick Gel Extraction Kit (250) (Qiagen, cat. no. 28706) RNeasy Mini Kit (50) (Qiagen, cat. no. 74104) PCR tubes, 0.5 ml (Eppendorf, cat. no. 951010057) PCR tubes, 0.2 ml thin-wall (MLS) Filtered pipettor tips (MLS) SYBR Green PCR Mastermix (Applied Biosystems, cat. no. 4334973) GeneAmp® dNTP Blend (100 mM) (Applied Biosystems, cat. no. N8080261) 3 M sodium acetate (pH 5.5) (Applied Biosystems/Ambion, cat. no. AM9740) 1 M Tris, pH 8.0 (100 ml) (Applied Biosystems/Ambion, AM9855G) Nuclease-free Water (1 L) (Applied Biosystems/Ambion, cat. no. AM9932) 10× NEBuffer2 (New England BioLabs® Inc., B7002S) Ethanol (Sigma-Aldrich®, cat. no. E7023) Ethylene glycol (American Bioanalytical, cat. no. AB00455-01000) Covaris microTUBE with AFA fiber and Snap-Cap with pre-slit Teflon/silicone/Teflon septa (Covaris™ Inc., cat. no. 520045) End-It™ DNA End-Repair Kit (Epicentre®, cat. no. ER0720) DNA Polymerase I (E. coli), (10U μl−1 ) (New England BioLabs® Inc., cat. no. M0209L) Quick Ligation™ kit (New England Biolabs, cat. no. M2200L) SYBR GreenER® qPCR SuperMix Universal (Invitrogen™ Corporation, cat. no. 11762-100) OR SYBR Green PCR Master Mix (Applied Biosystems, cat. no. 4309155) Agilent DNA 1000 Kit (Agilent Technologies, cat. no. 5067-1504) Agencourt® AMPure® 60 ml kit (Agencourt, cat. no. 000130) AmpliTaq® DNA Polymerase, LD with Buffer I (Applied Biosystems, cat. no. N8080157) Cloned Pfu polymerase (2.5 U μl−1 ) (Stratagene, cat. no. 600153) Invitrogen Platinum® PCR SuperMix (Invitrogen™ Corporation, cat. no. 11306-016) Quant-iT™ dsDNA HS Assay Kit, 100 assays (Invitrogen™ Corporation, cat. no. ) SOLiD™ Fragment Library Oligos Kit (Applied Biosystems, cat. no. 4401151) .. Brown-flaming micropipette puller (Sutter Instrument Co., Model P-80) Covaris™ S2 System, (for system materials summary, see “Covaris™ S2 System Materials Summary,” SOLiD ™ System 2.0 Site Preparation Guide .)

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    Thermo Fisher taq dna polymerase
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher absolute fast sybr green master mix
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