sybr green master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sybr green master mix
    Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green master mix/product/Thermo Fisher
    Average 99 stars, based on 1660 article reviews
    Price from $9.99 to $1999.99
    sybr green master mix - by Bioz Stars, 2020-01
    99/100 stars

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    Amplification:

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: .. The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ). .. Tested genes were described in previous publications from the literature [ – ] or selected here based on their predicted function; primers were designed with the use of Primer3 online software.

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer. .. Primer specificity and amplification efficiency were verified for each gene.

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: .. Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water. .. The amplification protocol was as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 30 sec and 60°C for 60 sec. Specificity was identified by melting-curve analysis.

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA). .. The qPCR conditions were: 50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles at 95°C for 15 s, and 60 °C for 1 min. Amplification of specific transcripts was confirmed by melting curve profiles generated at the end of the PCR program.

    Article Title: Epigenetic Regulation of the Ontogenic Expression of the Dopamine Transporter
    Article Snippet: A single PCR product of expected amplicon size was confirmed by agarose gel electrophoresis (data not shown). .. Reactions were prepared in a total volume of 25 µl using SYBR Green Master Mix (Applied Biosystems, Foster City, CA). qPCR was performed using the ViiA7™ Real-Time PCR system using the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 1 min at the annealing temperature for each primer set.

    Synthesized:

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: Complementary DNA (cDNA) was synthesized from RNA isolated using the SuperscriptTM II RNase H Reverse Transcriptase (Invitrogen, Sao Paulo, Brazil). .. The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer.

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: .. Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water. .. The amplification protocol was as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 30 sec and 60°C for 60 sec. Specificity was identified by melting-curve analysis.

    Article Title: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis
    Article Snippet: For miR-149-3p expression analysis, cDNA was synthesized from 50 ng extracted RNA using a miScript reverse transcription kit (Qiagen, Germany). .. SYBR Green Master Mix (Life Technologies) was used for gene expression level measurement.

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: For qPCR, total RNA was extracted using an RNeasy mini kit (Qiagen, Valencia, CA) and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). .. Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA).

    Quantitative RT-PCR:

    Article Title: PSPC1-interchanged interactions with PTK6 and β-catenin synergize oncogenic subcellular translocations and tumor progression
    Article Snippet: .. Quantitative RT-PCR was performed by using the SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s protocols. ..

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: Paragraph title: RT-qPCR validation of S . mansoni developmental genes ... The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ).

    Article Title: CDK2-mediated site-specific phosphorylation of EZH2 drives and maintains triple-negative breast cancer
    Article Snippet: Paragraph title: Quantitative real-time RT–PCR ... All PCR reactions were performed in triplicate with SYBR Green Master Mix (Applied Biosystems) in the presence of 1 μM of both the forward and reverse primer according to the manufacturer’s recommended thermocycling conditions, and then subjected to melt-curve analysis.

    Article Title: Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions
    Article Snippet: .. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) qRT-PCR analysis was performed by using SYBR Green Master Mix (Applied BioSystems, Foster City, CA, USA) following the manufacturer’s instructions [ ]. .. After treatment with Cav, THP-1 cells were lysed with Trizol (Invitrogen, Carlsbad, CA, USA).

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) ... Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water.

    Article Title: MicroRNA-181 inhibits the proliferation, drug sensitivity and invasion of human glioma cells by targeting Selenoprotein K (SELK)
    Article Snippet: .. Thereafter the quantitative RT-PCR was performed by using SYBR Green master mix (Applied Biosystems; Foster City, Calif, USA) on an ABI 7900HT system. .. The cycling conditions were as follows: 95°C for 20 sec, followed by 40 cycles of 95°C for 15 sec, and 58°C for 1 min. All reactions were carried out in triplicates and the gene expression was determined by the 2-ΔΔCt method.

    Article Title: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR ... SYBR Green Master Mix (Life Technologies) was used for gene expression level measurement.

    Article Title: Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling
    Article Snippet: .. The resulting cDNA was diluted 1:25 in water and used to perform qRT-PCR with SYBR green master mix (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: PSPC1-interchanged interactions with PTK6 and β-catenin synergize oncogenic subcellular translocations and tumor progression
    Article Snippet: .. Quantitative RT-PCR was performed by using the SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s protocols. ..

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: .. Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific). .. The antibodies and primers used in ChIP-qPCR assays are listed in .

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: .. The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ). .. Tested genes were described in previous publications from the literature [ – ] or selected here based on their predicted function; primers were designed with the use of Primer3 online software.

    Article Title: CDK2-mediated site-specific phosphorylation of EZH2 drives and maintains triple-negative breast cancer
    Article Snippet: .. All PCR reactions were performed in triplicate with SYBR Green Master Mix (Applied Biosystems) in the presence of 1 μM of both the forward and reverse primer according to the manufacturer’s recommended thermocycling conditions, and then subjected to melt-curve analysis. .. The quantity of the target gene for each sample was divided by the average sample quantity of the GAPDH to obtain the relative level of the gene expression.

    Article Title: Simvastatin Evokes An Unpredicted Antagonism For Tamoxifen In MCF-7 Breast Cancer Cells
    Article Snippet: .. Each RT-reaction served as a template in a 20 μL PCR reaction containing 0.2 μmol/L of each primer and SYBR green master mix (Thermo Fisher Scientific, UK). ..

    Article Title: Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions
    Article Snippet: .. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) qRT-PCR analysis was performed by using SYBR Green Master Mix (Applied BioSystems, Foster City, CA, USA) following the manufacturer’s instructions [ ]. .. After treatment with Cav, THP-1 cells were lysed with Trizol (Invitrogen, Carlsbad, CA, USA).

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: .. The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer. ..

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: .. Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water. .. The amplification protocol was as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 30 sec and 60°C for 60 sec. Specificity was identified by melting-curve analysis.

    Article Title: MicroRNA-181 inhibits the proliferation, drug sensitivity and invasion of human glioma cells by targeting Selenoprotein K (SELK)
    Article Snippet: .. Thereafter the quantitative RT-PCR was performed by using SYBR Green master mix (Applied Biosystems; Foster City, Calif, USA) on an ABI 7900HT system. .. The cycling conditions were as follows: 95°C for 20 sec, followed by 40 cycles of 95°C for 15 sec, and 58°C for 1 min. All reactions were carried out in triplicates and the gene expression was determined by the 2-ΔΔCt method.

    Article Title: Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function
    Article Snippet: .. Quantitative PCR (qPCR) was performed using either predesigned TaqMan probes or primers with SYBR Green Master Mix (Life Technologies) using a ViiA 7 RT-PCR system (Life Technologies). .. CYP1A1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (internal control) SYBR Green primers were obtained from Sigma-Aldrich as previously described, and miR-29b (000413 human miR-29b) and 001094 RNU44 internal control TaqMan primer/probes were purchased commercially (Life Technologies).

    Article Title: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis
    Article Snippet: .. SYBR Green Master Mix (Life Technologies) was used for gene expression level measurement. .. The qRT-PCR procedures were performed as follows: 50 °C for 2 min, 95 °C for 10 min, 35 cycles at 95 °C for 15 s, and 60 °C for 1 min.

    Article Title: Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α
    Article Snippet: .. Then, the SYBR Green Master Mix (Applied Biosystems, U.S.A.) was used to determine the relative expression levels of the genes under the reaction conditions as follows: an initial denaturation at 95°C for 30 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealed and extended at 60°C for 30 s. The relative mRNA levels were determined by the 2−ΔΔC T methods [ ]. β-actin served as a stable internal control. ..

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: .. Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA). .. The qPCR conditions were: 50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles at 95°C for 15 s, and 60 °C for 1 min. Amplification of specific transcripts was confirmed by melting curve profiles generated at the end of the PCR program.

    Article Title: Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling
    Article Snippet: .. The resulting cDNA was diluted 1:25 in water and used to perform qRT-PCR with SYBR green master mix (Applied Biosystems). ..

    Article Title: Epigenetic Regulation of the Ontogenic Expression of the Dopamine Transporter
    Article Snippet: .. Reactions were prepared in a total volume of 25 µl using SYBR Green Master Mix (Applied Biosystems, Foster City, CA). qPCR was performed using the ViiA7™ Real-Time PCR system using the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 1 min at the annealing temperature for each primer set. .. All samples were run in duplicate and analyzed using the 2−ΔΔCT ( ) with TATA binding protein used as a normalizer for each gene.

    Random Hexamer Labeling:

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: RT-qPCR validation of S . mansoni developmental genes For quantitative RT-PCR, complementary DNAs were obtained by reverse transcription (RT) of 50 ng adult worms total RNA using SuperScript IV Reverse Transcriptase (Invitrogen) and random hexamer primers in a 20 μL volume, according to the manufacturer’s recommendations. .. The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ).

    Expressing:

    Article Title: CDK2-mediated site-specific phosphorylation of EZH2 drives and maintains triple-negative breast cancer
    Article Snippet: All PCR reactions were performed in triplicate with SYBR Green Master Mix (Applied Biosystems) in the presence of 1 μM of both the forward and reverse primer according to the manufacturer’s recommended thermocycling conditions, and then subjected to melt-curve analysis. .. The quantity of the target gene for each sample was divided by the average sample quantity of the GAPDH to obtain the relative level of the gene expression.

    Article Title: Simvastatin Evokes An Unpredicted Antagonism For Tamoxifen In MCF-7 Breast Cancer Cells
    Article Snippet: Paragraph title: Determination Of mRNA Expression Of Bax And Bcl-2 ... Each RT-reaction served as a template in a 20 μL PCR reaction containing 0.2 μmol/L of each primer and SYBR green master mix (Thermo Fisher Scientific, UK).

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: For evaluation of gene expression levels for ABCG2, CX37 and CX43, the ovarian fragments were submitted to RNA extraction with TRIzol® Plus RNA Purification Kit (Invitrogen, São Paulo, SP, Brazil). .. The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer.

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: First-strand cDNA synthesis was performed on 1 μg of total RNA using GoScript™ Reverse Transcriptase (Promega, Beijing, China) according to the manufacturer's recommendations. qRT-PCR analysis was performed with the resulting cDNAs to assess the relative mRNA expression levels of BRD7 and GAPDH (an internal control). .. Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water.

    Article Title: MicroRNA-181 inhibits the proliferation, drug sensitivity and invasion of human glioma cells by targeting Selenoprotein K (SELK)
    Article Snippet: Thereafter the quantitative RT-PCR was performed by using SYBR Green master mix (Applied Biosystems; Foster City, Calif, USA) on an ABI 7900HT system. .. The cycling conditions were as follows: 95°C for 20 sec, followed by 40 cycles of 95°C for 15 sec, and 58°C for 1 min. All reactions were carried out in triplicates and the gene expression was determined by the 2-ΔΔCt method.

    Article Title: Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function
    Article Snippet: Quantitative PCR (qPCR) was performed using either predesigned TaqMan probes or primers with SYBR Green Master Mix (Life Technologies) using a ViiA 7 RT-PCR system (Life Technologies). .. Gene expression was normalized to an internal control (ΔCt = Ct gene of interest – Ct internal control).

    Article Title: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis
    Article Snippet: .. SYBR Green Master Mix (Life Technologies) was used for gene expression level measurement. .. The qRT-PCR procedures were performed as follows: 50 °C for 2 min, 95 °C for 10 min, 35 cycles at 95 °C for 15 s, and 60 °C for 1 min.

    Article Title: Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α
    Article Snippet: .. Then, the SYBR Green Master Mix (Applied Biosystems, U.S.A.) was used to determine the relative expression levels of the genes under the reaction conditions as follows: an initial denaturation at 95°C for 30 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealed and extended at 60°C for 30 s. The relative mRNA levels were determined by the 2−ΔΔC T methods [ ]. β-actin served as a stable internal control. ..

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA). .. Expression levels of target genes were normalized to the expression of 18S (internal control) and calculated based on the comparative cycle threshold (CT) method (2−ΔΔCt ).

    Western Blot:

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: Paragraph title: Western Blotting and qPCR ... Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA).

    Immunoprecipitation:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Diluted remains were subjected to second round immunoprecipitation against the second protein. .. Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: PSPC1-interchanged interactions with PTK6 and β-catenin synergize oncogenic subcellular translocations and tumor progression
    Article Snippet: RNA preparation and quantitative reversed transcription PCR Total cellular RNA was extracted using TRIzol™ reagent (Invitrogen) for RT-PCR. .. Quantitative RT-PCR was performed by using the SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s protocols.

    Article Title: Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions
    Article Snippet: .. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) qRT-PCR analysis was performed by using SYBR Green Master Mix (Applied BioSystems, Foster City, CA, USA) following the manufacturer’s instructions [ ]. .. After treatment with Cav, THP-1 cells were lysed with Trizol (Invitrogen, Carlsbad, CA, USA).

    Article Title: Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function
    Article Snippet: .. Quantitative PCR (qPCR) was performed using either predesigned TaqMan probes or primers with SYBR Green Master Mix (Life Technologies) using a ViiA 7 RT-PCR system (Life Technologies). .. CYP1A1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (internal control) SYBR Green primers were obtained from Sigma-Aldrich as previously described, and miR-29b (000413 human miR-29b) and 001094 RNU44 internal control TaqMan primer/probes were purchased commercially (Life Technologies).

    Article Title: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis
    Article Snippet: To detect mRNA expression of LINC00665 and RNF2, extracted RNA was reversely transcribed into cDNA by PrimeScript One-step RT-PCR kit (Takara, China). .. SYBR Green Master Mix (Life Technologies) was used for gene expression level measurement.

    Generated:

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA). .. The qPCR conditions were: 50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles at 95°C for 15 s, and 60 °C for 1 min. Amplification of specific transcripts was confirmed by melting curve profiles generated at the end of the PCR program.

    Sequencing:

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: .. Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water. .. The amplification protocol was as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 30 sec and 60°C for 60 sec. Specificity was identified by melting-curve analysis.

    Sonication:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Sonicated chromatin was diluted to a final volume of 1000 μl in ChIP lysis buffer supplemented with protease inhibitors before pre-clearing with 30 μl protein A Dynabeads (Thermo Fisher Scientific) for 2 h at 4°C. .. Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific).

    Binding Assay:

    Article Title: Epigenetic Regulation of the Ontogenic Expression of the Dopamine Transporter
    Article Snippet: Reactions were prepared in a total volume of 25 µl using SYBR Green Master Mix (Applied Biosystems, Foster City, CA). qPCR was performed using the ViiA7™ Real-Time PCR system using the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 1 min at the annealing temperature for each primer set. .. All samples were run in duplicate and analyzed using the 2−ΔΔCT ( ) with TATA binding protein used as a normalizer for each gene.

    ChIP-sequencing:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific). .. ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems).

    Isolation:

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: Complementary DNA (cDNA) was synthesized from RNA isolated using the SuperscriptTM II RNase H Reverse Transcriptase (Invitrogen, Sao Paulo, Brazil). .. The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer.

    Article Title: Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function
    Article Snippet: mRNA was isolated utilizing the Total RNA Purification Kit Plus (Norgen Biotek Corp.) Reverse transcription of mRNA was performed utilizing the Superscript VILO system (Life Technologies). .. Quantitative PCR (qPCR) was performed using either predesigned TaqMan probes or primers with SYBR Green Master Mix (Life Technologies) using a ViiA 7 RT-PCR system (Life Technologies).

    Article Title: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR ... SYBR Green Master Mix (Life Technologies) was used for gene expression level measurement.

    Size-exclusion Chromatography:

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water. .. The amplification protocol was as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 30 sec and 60°C for 60 sec. Specificity was identified by melting-curve analysis.

    Article Title: MicroRNA-181 inhibits the proliferation, drug sensitivity and invasion of human glioma cells by targeting Selenoprotein K (SELK)
    Article Snippet: Thereafter the quantitative RT-PCR was performed by using SYBR Green master mix (Applied Biosystems; Foster City, Calif, USA) on an ABI 7900HT system. .. The cycling conditions were as follows: 95°C for 20 sec, followed by 40 cycles of 95°C for 15 sec, and 58°C for 1 min. All reactions were carried out in triplicates and the gene expression was determined by the 2-ΔΔCt method.

    Purification:

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: For evaluation of gene expression levels for ABCG2, CX37 and CX43, the ovarian fragments were submitted to RNA extraction with TRIzol® Plus RNA Purification Kit (Invitrogen, São Paulo, SP, Brazil). .. The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer.

    Article Title: Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function
    Article Snippet: mRNA was isolated utilizing the Total RNA Purification Kit Plus (Norgen Biotek Corp.) Reverse transcription of mRNA was performed utilizing the Superscript VILO system (Life Technologies). .. Quantitative PCR (qPCR) was performed using either predesigned TaqMan probes or primers with SYBR Green Master Mix (Life Technologies) using a ViiA 7 RT-PCR system (Life Technologies).

    Polymerase Chain Reaction:

    Article Title: PSPC1-interchanged interactions with PTK6 and β-catenin synergize oncogenic subcellular translocations and tumor progression
    Article Snippet: Paragraph title: RNA preparation and quantitative reversed transcription PCR ... Quantitative RT-PCR was performed by using the SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s protocols.

    Article Title: CDK2-mediated site-specific phosphorylation of EZH2 drives and maintains triple-negative breast cancer
    Article Snippet: .. All PCR reactions were performed in triplicate with SYBR Green Master Mix (Applied Biosystems) in the presence of 1 μM of both the forward and reverse primer according to the manufacturer’s recommended thermocycling conditions, and then subjected to melt-curve analysis. .. The quantity of the target gene for each sample was divided by the average sample quantity of the GAPDH to obtain the relative level of the gene expression.

    Article Title: Simvastatin Evokes An Unpredicted Antagonism For Tamoxifen In MCF-7 Breast Cancer Cells
    Article Snippet: .. Each RT-reaction served as a template in a 20 μL PCR reaction containing 0.2 μmol/L of each primer and SYBR green master mix (Thermo Fisher Scientific, UK). ..

    Article Title: Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions
    Article Snippet: Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) qRT-PCR analysis was performed by using SYBR Green Master Mix (Applied BioSystems, Foster City, CA, USA) following the manufacturer’s instructions [ ]. .. Each sample contains 10 ng of cDNA, 1× Master Mix, and gene-specific primers at a final concentration of 100 nM in a 20-μL total volume and was used for PCR.

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) ... Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water.

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA). .. The qPCR conditions were: 50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles at 95°C for 15 s, and 60 °C for 1 min. Amplification of specific transcripts was confirmed by melting curve profiles generated at the end of the PCR program.

    Article Title: Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling
    Article Snippet: Samples were diluted to 10 ng/μL for mtDNA copy number analysis by PCR using mtDNA and nuclear DNA primers as described ( ). .. The resulting cDNA was diluted 1:25 in water and used to perform qRT-PCR with SYBR green master mix (Applied Biosystems).

    Article Title: Epigenetic Regulation of the Ontogenic Expression of the Dopamine Transporter
    Article Snippet: A single PCR product of expected amplicon size was confirmed by agarose gel electrophoresis (data not shown). .. Reactions were prepared in a total volume of 25 µl using SYBR Green Master Mix (Applied Biosystems, Foster City, CA). qPCR was performed using the ViiA7™ Real-Time PCR system using the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 1 min at the annealing temperature for each primer set.

    Blocking Assay:

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: After blocking with 5 % BSA, membranes were probed with primary antibodies to STAT1 (#9172), pSTAT1 (#9167), β-actin (8H10D10, all Cell Signaling, Danvers, MA). .. Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA).

    Chromatin Immunoprecipitation:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Paragraph title: Chromatin immunoprecipitation (ChIP) and Re-ChIP qPCR ... Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific).

    Software:

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ). .. Tested genes were described in previous publications from the literature [ – ] or selected here based on their predicted function; primers were designed with the use of Primer3 online software.

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water. .. The crossing threshold (Ct) value was obtained during the exponential amplification phase using the instrument's software (SDS v.2.3).

    Real-time Polymerase Chain Reaction:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: .. Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific). .. The antibodies and primers used in ChIP-qPCR assays are listed in .

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: .. The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ). .. Tested genes were described in previous publications from the literature [ – ] or selected here based on their predicted function; primers were designed with the use of Primer3 online software.

    Article Title: Simvastatin Evokes An Unpredicted Antagonism For Tamoxifen In MCF-7 Breast Cancer Cells
    Article Snippet: Real-time PCR analysis was conducted using the thermocycler Step One™ (Applied Biosystems). .. Each RT-reaction served as a template in a 20 μL PCR reaction containing 0.2 μmol/L of each primer and SYBR green master mix (Thermo Fisher Scientific, UK).

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: .. The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer. ..

    Article Title: Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function
    Article Snippet: .. Quantitative PCR (qPCR) was performed using either predesigned TaqMan probes or primers with SYBR Green Master Mix (Life Technologies) using a ViiA 7 RT-PCR system (Life Technologies). .. CYP1A1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (internal control) SYBR Green primers were obtained from Sigma-Aldrich as previously described, and miR-29b (000413 human miR-29b) and 001094 RNU44 internal control TaqMan primer/probes were purchased commercially (Life Technologies).

    Article Title: Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α
    Article Snippet: Paragraph title: Real-time quantitative PCR ... Then, the SYBR Green Master Mix (Applied Biosystems, U.S.A.) was used to determine the relative expression levels of the genes under the reaction conditions as follows: an initial denaturation at 95°C for 30 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealed and extended at 60°C for 30 s. The relative mRNA levels were determined by the 2−ΔΔC T methods [ ]. β-actin served as a stable internal control.

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: Paragraph title: Western Blotting and qPCR ... Each reaction mixture contained 10 μl of SYBR Green Master Mix (Applied Biosystems), 0.5 μl of forward and reverse primers (Invitrogen) and 3 μl of cDNA (corresponding to 50 ng of RNA).

    Article Title: Epigenetic Regulation of the Ontogenic Expression of the Dopamine Transporter
    Article Snippet: .. Reactions were prepared in a total volume of 25 µl using SYBR Green Master Mix (Applied Biosystems, Foster City, CA). qPCR was performed using the ViiA7™ Real-Time PCR system using the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 1 min at the annealing temperature for each primer set. .. All samples were run in duplicate and analyzed using the 2−ΔΔCT ( ) with TATA binding protein used as a normalizer for each gene.

    RNA Extraction:

    Article Title: Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles
    Article Snippet: Paragraph title: 4.11. Ribonucleic Acid (RNA) Extraction and Quantitative Real-Time PCR ... The qPCR reactions had a final volume of 20 μL and contained the following components: 1 μL cDNA as template in 7.5 μL SYBR Green Master Mix (PE Applied Biosystems, Foster City, CA, USA), 5.5 μL of ultrapure water and 0.5 μL of each primer.

    Article Title: Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) ... Gene-specific amplification was performed in an ABI Prism 7900HT Sequence Detection System (Life Technologies, Carlsbad, CA, USA) in a final volume of 15 μl SYBR Green Master Mix, which contained the following: 0.5 μl of cDNA (synthesized as described above), 7.5 μl of 2x SYBR Green Master Mix (Invitrogen, USA), 2 μl of each pair of oligonucleotide primers and 5 μl of nuclease-free water.

    Agarose Gel Electrophoresis:

    Article Title: Epigenetic Regulation of the Ontogenic Expression of the Dopamine Transporter
    Article Snippet: A single PCR product of expected amplicon size was confirmed by agarose gel electrophoresis (data not shown). .. Reactions were prepared in a total volume of 25 µl using SYBR Green Master Mix (Applied Biosystems, Foster City, CA). qPCR was performed using the ViiA7™ Real-Time PCR system using the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 1 min at the annealing temperature for each primer set.

    Incubation:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: The DNA sample was incubated overnight at 65°C to reverse crosslinks, diluted 2-fold in 50 mM Tris, pH 8, plus 10 mM EDTA, and then sequentially digested with RNase A for 2 h at 37°C and proteinase K at 55°C. .. Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific).

    Spectrophotometry:

    Article Title: Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling
    Article Snippet: Samples were centrifuged at 13,000 g for 1 minute, and the DNA concentration in the supernatant was measured using a nanodrop spectrophotometer. .. The resulting cDNA was diluted 1:25 in water and used to perform qRT-PCR with SYBR green master mix (Applied Biosystems).

    Concentration Assay:

    Article Title: Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions
    Article Snippet: Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) qRT-PCR analysis was performed by using SYBR Green Master Mix (Applied BioSystems, Foster City, CA, USA) following the manufacturer’s instructions [ ]. .. Each sample contains 10 ng of cDNA, 1× Master Mix, and gene-specific primers at a final concentration of 100 nM in a 20-μL total volume and was used for PCR.

    Article Title: Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling
    Article Snippet: Samples were centrifuged at 13,000 g for 1 minute, and the DNA concentration in the supernatant was measured using a nanodrop spectrophotometer. .. The resulting cDNA was diluted 1:25 in water and used to perform qRT-PCR with SYBR green master mix (Applied Biosystems).

    Lysis:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Sample was washed three times with ChIP lysis buffer, and eluted in elution buffer (50 mM Tris, pH 8, 10 mM EDTA, 1% SDS) at 65°C for 15 min. For Re-ChIP-qPCR assay, the samples after de-crosslink were subjected to qPCR assay and remains were diluted with ChIP lysis buffer. .. Then, DNA enrichment was quantified by real-time PCR (ABI 7900) using SYBR Green Master Mix (Thermo Fisher Scientific).

    T-Test:

    Article Title: In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms
    Article Snippet: The resulting cDNA was diluted 8-fold in water and qPCR amplification was done with 2.5 μL of diluted cDNA in a total volume of 10 μL using SYBR Green Master Mix (Life Technologies) and specific primer pairs ( ). .. Results were analyzed by comparative Ct method and the statistical significance was calculated with the two-sided t-test.

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