sybr green i staining  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Thermo Fisher sybr green i staining
    Effect of Cre- loxP system on the concatemer production in RCR. pUC_OriC300 (− loxP ) or pUC_OLDT (+ loxP ) (0.05 ng) was incubated in the RCR mixture at 33 °C for 3 h in the absence (0 mU) or presence (5, 15, 50, or 150 mU) of Cre recombinase. The product was analyzed by 0.5% TBE-agarose gel electrophoresis and <t>SYBR</t> <t>Green</t> I staining. The ratio of concatemers to the sum of concatemers and supercoils is shown in a graph.
    Sybr Green I Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i staining/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green i staining - by Bioz Stars, 2022-09
    88/100 stars

    Images

    1) Product Images from "Efficient Arrangement of the Replication Fork Trap for In Vitro Propagation of Monomeric Circular DNA in the Chromosome-Replication Cycle Reaction"

    Article Title: Efficient Arrangement of the Replication Fork Trap for In Vitro Propagation of Monomeric Circular DNA in the Chromosome-Replication Cycle Reaction

    Journal: Life

    doi: 10.3390/life8040043

    Effect of Cre- loxP system on the concatemer production in RCR. pUC_OriC300 (− loxP ) or pUC_OLDT (+ loxP ) (0.05 ng) was incubated in the RCR mixture at 33 °C for 3 h in the absence (0 mU) or presence (5, 15, 50, or 150 mU) of Cre recombinase. The product was analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The ratio of concatemers to the sum of concatemers and supercoils is shown in a graph.
    Figure Legend Snippet: Effect of Cre- loxP system on the concatemer production in RCR. pUC_OriC300 (− loxP ) or pUC_OLDT (+ loxP ) (0.05 ng) was incubated in the RCR mixture at 33 °C for 3 h in the absence (0 mU) or presence (5, 15, 50, or 150 mU) of Cre recombinase. The product was analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The ratio of concatemers to the sum of concatemers and supercoils is shown in a graph.

    Techniques Used: Incubation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining

    Effect of Tus on the RCR propagation of circular DNA in which the oriC-ter arrangement mimics the chromosome position. pKZter_1 (+ ter ) is a pKOZ ( −ter ) derivative containing inward-facing ter sites opposite oriC . The regions available for fork progression from the oriC cassette are indicated by dotted arrows on the circular map. pPKOZ or pKZter_1 (2.5 ng) was incubated in the RCR mixture at 30 °C for 3 h in the presence of the indicated concentrations of Tus. Aliquots (0.2 µL) were analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The linear DNA size marker fragments were derived from lambda phage DNA (marker).
    Figure Legend Snippet: Effect of Tus on the RCR propagation of circular DNA in which the oriC-ter arrangement mimics the chromosome position. pKZter_1 (+ ter ) is a pKOZ ( −ter ) derivative containing inward-facing ter sites opposite oriC . The regions available for fork progression from the oriC cassette are indicated by dotted arrows on the circular map. pPKOZ or pKZter_1 (2.5 ng) was incubated in the RCR mixture at 30 °C for 3 h in the presence of the indicated concentrations of Tus. Aliquots (0.2 µL) were analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The linear DNA size marker fragments were derived from lambda phage DNA (marker).

    Techniques Used: Incubation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Marker, Derivative Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Thermo Fisher sybr green i staining
    Effect of Cre- loxP system on the concatemer production in RCR. pUC_OriC300 (− loxP ) or pUC_OLDT (+ loxP ) (0.05 ng) was incubated in the RCR mixture at 33 °C for 3 h in the absence (0 mU) or presence (5, 15, 50, or 150 mU) of Cre recombinase. The product was analyzed by 0.5% TBE-agarose gel electrophoresis and <t>SYBR</t> <t>Green</t> I staining. The ratio of concatemers to the sum of concatemers and supercoils is shown in a graph.
    Sybr Green I Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i staining/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green i staining - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybr green i
    Representative images of 1 day (A), 2 day (B), 3 day (C), 4 day (D), 5 day (E) and 6 day (F) old B. henselae cultures. The Bartonella henselae cultures of varying ages were stained with <t>SYBR</t> Green I/PI assay and observed under the fluorescence microscope (400 × magnification). The bacterial cells were stained as green by SYBR <t>Green</t> I
    Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of Cre- loxP system on the concatemer production in RCR. pUC_OriC300 (− loxP ) or pUC_OLDT (+ loxP ) (0.05 ng) was incubated in the RCR mixture at 33 °C for 3 h in the absence (0 mU) or presence (5, 15, 50, or 150 mU) of Cre recombinase. The product was analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The ratio of concatemers to the sum of concatemers and supercoils is shown in a graph.

    Journal: Life

    Article Title: Efficient Arrangement of the Replication Fork Trap for In Vitro Propagation of Monomeric Circular DNA in the Chromosome-Replication Cycle Reaction

    doi: 10.3390/life8040043

    Figure Lengend Snippet: Effect of Cre- loxP system on the concatemer production in RCR. pUC_OriC300 (− loxP ) or pUC_OLDT (+ loxP ) (0.05 ng) was incubated in the RCR mixture at 33 °C for 3 h in the absence (0 mU) or presence (5, 15, 50, or 150 mU) of Cre recombinase. The product was analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The ratio of concatemers to the sum of concatemers and supercoils is shown in a graph.

    Article Snippet: An aliquot (2 µL) was analyzed by 0.5% or 1.0% agarose gel electrophoresis, followed by SYBR Green I staining (Molecular Probes, Eugene, USA).

    Techniques: Incubation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining

    Effect of Tus on the RCR propagation of circular DNA in which the oriC-ter arrangement mimics the chromosome position. pKZter_1 (+ ter ) is a pKOZ ( −ter ) derivative containing inward-facing ter sites opposite oriC . The regions available for fork progression from the oriC cassette are indicated by dotted arrows on the circular map. pPKOZ or pKZter_1 (2.5 ng) was incubated in the RCR mixture at 30 °C for 3 h in the presence of the indicated concentrations of Tus. Aliquots (0.2 µL) were analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The linear DNA size marker fragments were derived from lambda phage DNA (marker).

    Journal: Life

    Article Title: Efficient Arrangement of the Replication Fork Trap for In Vitro Propagation of Monomeric Circular DNA in the Chromosome-Replication Cycle Reaction

    doi: 10.3390/life8040043

    Figure Lengend Snippet: Effect of Tus on the RCR propagation of circular DNA in which the oriC-ter arrangement mimics the chromosome position. pKZter_1 (+ ter ) is a pKOZ ( −ter ) derivative containing inward-facing ter sites opposite oriC . The regions available for fork progression from the oriC cassette are indicated by dotted arrows on the circular map. pPKOZ or pKZter_1 (2.5 ng) was incubated in the RCR mixture at 30 °C for 3 h in the presence of the indicated concentrations of Tus. Aliquots (0.2 µL) were analyzed by 0.5% TBE-agarose gel electrophoresis and SYBR Green I staining. The linear DNA size marker fragments were derived from lambda phage DNA (marker).

    Article Snippet: An aliquot (2 µL) was analyzed by 0.5% or 1.0% agarose gel electrophoresis, followed by SYBR Green I staining (Molecular Probes, Eugene, USA).

    Techniques: Incubation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Marker, Derivative Assay

    Representative images of 1 day (A), 2 day (B), 3 day (C), 4 day (D), 5 day (E) and 6 day (F) old B. henselae cultures. The Bartonella henselae cultures of varying ages were stained with SYBR Green I/PI assay and observed under the fluorescence microscope (400 × magnification). The bacterial cells were stained as green by SYBR Green I

    Journal: BMC Microbiology

    Article Title: Effect of different drugs and drug combinations on killing stationary phase and biofilms recovered cells of Bartonella henselae in vitro

    doi: 10.1186/s12866-020-01777-9

    Figure Lengend Snippet: Representative images of 1 day (A), 2 day (B), 3 day (C), 4 day (D), 5 day (E) and 6 day (F) old B. henselae cultures. The Bartonella henselae cultures of varying ages were stained with SYBR Green I/PI assay and observed under the fluorescence microscope (400 × magnification). The bacterial cells were stained as green by SYBR Green I

    Article Snippet: 10 μL SYBR Green I (100× stock, Invitrogen, Waltham, MA, USA) and propidium iodide (PI, 600 μM, Sigma, St. Louis MO, USA) staining mixture was added to each well and mixed thoroughly.

    Techniques: Staining, SYBR Green Assay, Fluorescence, Microscopy

    Biparametric flow cytrometry virus plots. Four different viral populations were discriminated combining side scatter signal vs. green fluorescent signal after staining with SYBR Green I (reflecting the amount of DNA and hence genome sizes): I, Small viruses, II, medium viruses, III, large viruses, IV, huge viruses ( A ) (see also Figure S2 ). Abundance of small (I), medium (II), large (III), and huge (IV) viruses determined by FCM in PAME-I ( B ). Blue circles show no addition of carbon in the form of glucose, while red circles show three Redfield additions of carbon. Open and filled circles display perturbations with or without silicate (open: no Si addition; filled: Si addition). See Figure 2 for more information about experimental design.

    Journal: Viruses

    Article Title: The Response of Heterotrophic Prokaryote and Viral Communities to Labile Organic Carbon Inputs Is Controlled by the Predator Food Chain Structure

    doi: 10.3390/v9090238

    Figure Lengend Snippet: Biparametric flow cytrometry virus plots. Four different viral populations were discriminated combining side scatter signal vs. green fluorescent signal after staining with SYBR Green I (reflecting the amount of DNA and hence genome sizes): I, Small viruses, II, medium viruses, III, large viruses, IV, huge viruses ( A ) (see also Figure S2 ). Abundance of small (I), medium (II), large (III), and huge (IV) viruses determined by FCM in PAME-I ( B ). Blue circles show no addition of carbon in the form of glucose, while red circles show three Redfield additions of carbon. Open and filled circles display perturbations with or without silicate (open: no Si addition; filled: Si addition). See Figure 2 for more information about experimental design.

    Article Snippet: Enumeration of virus-like particles was performed on samples fixed with glutaraldehyde (final concentration 0.5% v /v ) prior to staining with 1 × SYBR Green I (Molecular Probes, Eugene, OR, USA).

    Techniques: Flow Cytometry, Staining, SYBR Green Assay

    Representative flow cytometry plots showing E. huxleyi cells, inoculated and non-inoculated with viruses (20 hours PI) (A) without fluorescence dye, or stained with the fluorescence dyes (B) lipid-specific dye FM 1-43, (C) CM-H 2 DCFDA for detection of accumulated Reactive Oxygen Species in cells and (D) DNA dye SYBR Green I. Infected and non-infected cells were discriminated on the basis of their red autofluorescence (610 nm) or the green fluorescence (522 nm) of SYBR Green I and CM-H 2 DCFDA versus side scatter, green dye fluorescence or orange lipid dye FM 1-43 fluorescence (488 nm).

    Journal: PLoS ONE

    Article Title: Targeted Sorting of Single Virus-Infected Cells of the Coccolithophore Emiliania huxleyi

    doi: 10.1371/journal.pone.0022520

    Figure Lengend Snippet: Representative flow cytometry plots showing E. huxleyi cells, inoculated and non-inoculated with viruses (20 hours PI) (A) without fluorescence dye, or stained with the fluorescence dyes (B) lipid-specific dye FM 1-43, (C) CM-H 2 DCFDA for detection of accumulated Reactive Oxygen Species in cells and (D) DNA dye SYBR Green I. Infected and non-infected cells were discriminated on the basis of their red autofluorescence (610 nm) or the green fluorescence (522 nm) of SYBR Green I and CM-H 2 DCFDA versus side scatter, green dye fluorescence or orange lipid dye FM 1-43 fluorescence (488 nm).

    Article Snippet: 1.4×105 cells ml∼1 ) were placed into microcentrifuge Eppendorf tubes and labeled with either fluorescent dye N-(3-riethylammoniumpropyl)-4-[4-(dibutylamino)styryl] pyridinium dibromide (FM 1-43, Invitrogen Co., Carlsbad, CA, USA), 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2 DCFDA, Molecular Probes Inc., Eugene, OR, USA) or SYBR Green I (Molecular Probes Inc., Eugene, OR, USA).

    Techniques: Flow Cytometry, Cytometry, Fluorescence, Staining, SYBR Green Assay, Infection