sybr green i  (Millipore)


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    Structured Review

    Millipore sybr green i
    <t>SYBR</t> <t>Green</t> I nuclear staining of Sporothrix spp. Fluorescence microscopy analysis of (A) S. brasiliensis ATCC MYA-4823, (B) S. schenckii ATCC MYA-4821, and (C) S. pallida MUM 17.04 stained with SYBR Green I reveals the presence of mononucleated yeast cells. Images (1,036 × 1,024 pixels) were acquired in a fluorescence microscope (DP71 Olympus) with an oil immersion objective (100×/1.4) and cropped using the cellSens imaging software (Olympus Life Science). Scale bar equals 3 μm.
    Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i/product/Millipore
    Average 97 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2020-02
    97/100 stars

    Images

    1) Product Images from "Ploidy Determination in the Pathogenic Fungus Sporothrix spp."

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.00284

    SYBR Green I nuclear staining of Sporothrix spp. Fluorescence microscopy analysis of (A) S. brasiliensis ATCC MYA-4823, (B) S. schenckii ATCC MYA-4821, and (C) S. pallida MUM 17.04 stained with SYBR Green I reveals the presence of mononucleated yeast cells. Images (1,036 × 1,024 pixels) were acquired in a fluorescence microscope (DP71 Olympus) with an oil immersion objective (100×/1.4) and cropped using the cellSens imaging software (Olympus Life Science). Scale bar equals 3 μm.
    Figure Legend Snippet: SYBR Green I nuclear staining of Sporothrix spp. Fluorescence microscopy analysis of (A) S. brasiliensis ATCC MYA-4823, (B) S. schenckii ATCC MYA-4821, and (C) S. pallida MUM 17.04 stained with SYBR Green I reveals the presence of mononucleated yeast cells. Images (1,036 × 1,024 pixels) were acquired in a fluorescence microscope (DP71 Olympus) with an oil immersion objective (100×/1.4) and cropped using the cellSens imaging software (Olympus Life Science). Scale bar equals 3 μm.

    Techniques Used: SYBR Green Assay, Staining, Fluorescence, Microscopy, Imaging, Software

    Related Articles

    SYBR Green Assay:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany). .. Results were exported as FCS files to FlowJo (Tree Star Inc, USA) and gated identically for all samples with green fluorescence (533 ± 30 nm) and red fluorescence ( > 670 nm).

    Article Title: Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci
    Article Snippet: .. After staining with 1 × SYBR Green I and 40 μg/mL propidium iodide, samples were incubated at room temperature for 25 min in the dark, harvested on GTBP filters (Ø = 0.2 μm, Millipore, Billerica, MA, USA), and examined under an epifluorescence microscope (Axioskop 2, Zeiss, Milano, Italy). .. CHECKERBOARD TEST Synergy was tested by the checkerboard method, a two-dimensional array of serial concentrations of test compounds, that has been used most frequently to assess antimicrobial combinations in vitro ( ).

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.
    Article Snippet: .. For the yeast cells, a concentration of SYBR Green I of 2% (vol/vol) was used from a 10-fold diluted working solution in Tris-EDTA (pH 8.0) (Sigma-Aldrich). .. For the case of conidia, a final concentration of 12% (vol/vol) of SYBR Green I (10,000×) was used.

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Article Title: Mesoscale distribution and functional diversity of picoeukaryotes in the first-year sea ice of the Canadian Arctic
    Article Snippet: .. They were thawed in a 30 °C water bath and stained with SYBR-Green I in tris-EDTA buffer (10 mℳTris, 1 mℳEDTA; Sigma) ( , ). .. After cooling to ambient temperature, subsamples were analyzed with a FACSort flow cytometer (Becton and Dickinson, San Jose, CA, USA) equipped with an air-cooled argon laser (15 mW).

    Article Title: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes
    Article Snippet: .. The obtained cDNA was used in qRT-PCR, which was performed in a 20-μL reaction mixture that contained one unit of Taq polymerase (Fermentas, Canada), 3.5 mM of MgCl2 , 1 unit of PCR buffer, 0.5 mM of each primer, 10 mM dNTPs, 0.5 units of SYBR Green I, 0.6 mL of DMSO (Sigma-Aldrich), and 2 μL of template cDNA, using a Roter-Gene Real-Time thermocycler (QIAGEN, USA). .. The PCR conditions were as follows: 95 °C for 1 min, followed by 40 cycles of 95 °C for 15 s, 58–60 °C for 30 s and 72 °C for 30 s. The relative expression of the cold-responsive genes (CBF3 and COR47) was calculated using the 2−ΔΔCt comparative CT method (Livak and Schmittgen ).

    Article Title: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
    Article Snippet: .. At 0 h, 24 h, 48 h, 72 h, 96 h, and 120 h, 5 μl of the culture was stained in 95 μl of D-PBS (Gibco) supplemented with 2× Sybr Green I (Ozyme; stock = 10,000×) for 30 min at room temperature, diluted 20-fold in D-PBS (final volume = 200 μl), and the Sybr Green fluorescence measured in a Guava easyCyte Flow Cytometer (EMD Millipore). ..

    Flow Cytometry:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: FCM analysis FCM analysis was performed according to Prest et al. using a BD Accuri C6 flow cytometer (Becton Dickinson, Belgium) equipped with a 50 mW laser, emission wavelength at 488 nm. .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany).

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.
    Article Snippet: For the yeast cells, a concentration of SYBR Green I of 2% (vol/vol) was used from a 10-fold diluted working solution in Tris-EDTA (pH 8.0) (Sigma-Aldrich). .. Prior to flow cytometry (FCM) analysis, 750 μl of sodium citrate buffer (50 mM; pH 7.5) with 0.25% (vol/vol) of Triton X-100 (Sigma-Aldrich) was added to the stained cells.

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Article Title: Mesoscale distribution and functional diversity of picoeukaryotes in the first-year sea ice of the Canadian Arctic
    Article Snippet: They were thawed in a 30 °C water bath and stained with SYBR-Green I in tris-EDTA buffer (10 mℳTris, 1 mℳEDTA; Sigma) ( , ). .. After cooling to ambient temperature, subsamples were analyzed with a FACSort flow cytometer (Becton and Dickinson, San Jose, CA, USA) equipped with an air-cooled argon laser (15 mW).

    Article Title: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
    Article Snippet: .. At 0 h, 24 h, 48 h, 72 h, 96 h, and 120 h, 5 μl of the culture was stained in 95 μl of D-PBS (Gibco) supplemented with 2× Sybr Green I (Ozyme; stock = 10,000×) for 30 min at room temperature, diluted 20-fold in D-PBS (final volume = 200 μl), and the Sybr Green fluorescence measured in a Guava easyCyte Flow Cytometer (EMD Millipore). ..

    Immunocytochemistry:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany). .. Results were exported as FCS files to FlowJo (Tree Star Inc, USA) and gated identically for all samples with green fluorescence (533 ± 30 nm) and red fluorescence ( > 670 nm).

    Fluorescence:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany). .. Results were exported as FCS files to FlowJo (Tree Star Inc, USA) and gated identically for all samples with green fluorescence (533 ± 30 nm) and red fluorescence ( > 670 nm).

    Article Title: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
    Article Snippet: .. At 0 h, 24 h, 48 h, 72 h, 96 h, and 120 h, 5 μl of the culture was stained in 95 μl of D-PBS (Gibco) supplemented with 2× Sybr Green I (Ozyme; stock = 10,000×) for 30 min at room temperature, diluted 20-fold in D-PBS (final volume = 200 μl), and the Sybr Green fluorescence measured in a Guava easyCyte Flow Cytometer (EMD Millipore). ..

    Polymerase Chain Reaction:

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). .. Phylogenetic analyses of growth-positive cultures were based on PCR amplification and sequencing of 16 S rRNA gene sequences.

    Article Title: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes
    Article Snippet: .. The obtained cDNA was used in qRT-PCR, which was performed in a 20-μL reaction mixture that contained one unit of Taq polymerase (Fermentas, Canada), 3.5 mM of MgCl2 , 1 unit of PCR buffer, 0.5 mM of each primer, 10 mM dNTPs, 0.5 units of SYBR Green I, 0.6 mL of DMSO (Sigma-Aldrich), and 2 μL of template cDNA, using a Roter-Gene Real-Time thermocycler (QIAGEN, USA). .. The PCR conditions were as follows: 95 °C for 1 min, followed by 40 cycles of 95 °C for 15 s, 58–60 °C for 30 s and 72 °C for 30 s. The relative expression of the cold-responsive genes (CBF3 and COR47) was calculated using the 2−ΔΔCt comparative CT method (Livak and Schmittgen ).

    Cytometry:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: FCM analysis FCM analysis was performed according to Prest et al. using a BD Accuri C6 flow cytometer (Becton Dickinson, Belgium) equipped with a 50 mW laser, emission wavelength at 488 nm. .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany).

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.
    Article Snippet: For the yeast cells, a concentration of SYBR Green I of 2% (vol/vol) was used from a 10-fold diluted working solution in Tris-EDTA (pH 8.0) (Sigma-Aldrich). .. Prior to flow cytometry (FCM) analysis, 750 μl of sodium citrate buffer (50 mM; pH 7.5) with 0.25% (vol/vol) of Triton X-100 (Sigma-Aldrich) was added to the stained cells.

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Article Title: Mesoscale distribution and functional diversity of picoeukaryotes in the first-year sea ice of the Canadian Arctic
    Article Snippet: They were thawed in a 30 °C water bath and stained with SYBR-Green I in tris-EDTA buffer (10 mℳTris, 1 mℳEDTA; Sigma) ( , ). .. After cooling to ambient temperature, subsamples were analyzed with a FACSort flow cytometer (Becton and Dickinson, San Jose, CA, USA) equipped with an air-cooled argon laser (15 mW).

    Article Title: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
    Article Snippet: .. At 0 h, 24 h, 48 h, 72 h, 96 h, and 120 h, 5 μl of the culture was stained in 95 μl of D-PBS (Gibco) supplemented with 2× Sybr Green I (Ozyme; stock = 10,000×) for 30 min at room temperature, diluted 20-fold in D-PBS (final volume = 200 μl), and the Sybr Green fluorescence measured in a Guava easyCyte Flow Cytometer (EMD Millipore). ..

    Microscopy:

    Article Title: Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci
    Article Snippet: .. After staining with 1 × SYBR Green I and 40 μg/mL propidium iodide, samples were incubated at room temperature for 25 min in the dark, harvested on GTBP filters (Ø = 0.2 μm, Millipore, Billerica, MA, USA), and examined under an epifluorescence microscope (Axioskop 2, Zeiss, Milano, Italy). .. CHECKERBOARD TEST Synergy was tested by the checkerboard method, a two-dimensional array of serial concentrations of test compounds, that has been used most frequently to assess antimicrobial combinations in vitro ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes
    Article Snippet: Paragraph title: Real-time PCR analysis ... The obtained cDNA was used in qRT-PCR, which was performed in a 20-μL reaction mixture that contained one unit of Taq polymerase (Fermentas, Canada), 3.5 mM of MgCl2 , 1 unit of PCR buffer, 0.5 mM of each primer, 10 mM dNTPs, 0.5 units of SYBR Green I, 0.6 mL of DMSO (Sigma-Aldrich), and 2 μL of template cDNA, using a Roter-Gene Real-Time thermocycler (QIAGEN, USA).

    Concentration Assay:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany). .. Results were exported as FCS files to FlowJo (Tree Star Inc, USA) and gated identically for all samples with green fluorescence (533 ± 30 nm) and red fluorescence ( > 670 nm).

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.
    Article Snippet: .. For the yeast cells, a concentration of SYBR Green I of 2% (vol/vol) was used from a 10-fold diluted working solution in Tris-EDTA (pH 8.0) (Sigma-Aldrich). .. For the case of conidia, a final concentration of 12% (vol/vol) of SYBR Green I (10,000×) was used.

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Article Title: Mesoscale distribution and functional diversity of picoeukaryotes in the first-year sea ice of the Canadian Arctic
    Article Snippet: Samples for bacterial numbers were prefiltered through 40 μm plankton net, preserved with glutaraldehyde (final concentration 0.1%) and frozen in liquid nitrogen. .. They were thawed in a 30 °C water bath and stained with SYBR-Green I in tris-EDTA buffer (10 mℳTris, 1 mℳEDTA; Sigma) ( , ).

    Incubation:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany). .. Results were exported as FCS files to FlowJo (Tree Star Inc, USA) and gated identically for all samples with green fluorescence (533 ± 30 nm) and red fluorescence ( > 670 nm).

    Article Title: Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci
    Article Snippet: .. After staining with 1 × SYBR Green I and 40 μg/mL propidium iodide, samples were incubated at room temperature for 25 min in the dark, harvested on GTBP filters (Ø = 0.2 μm, Millipore, Billerica, MA, USA), and examined under an epifluorescence microscope (Axioskop 2, Zeiss, Milano, Italy). .. CHECKERBOARD TEST Synergy was tested by the checkerboard method, a two-dimensional array of serial concentrations of test compounds, that has been used most frequently to assess antimicrobial combinations in vitro ( ).

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Amplification:

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). .. Phylogenetic analyses of growth-positive cultures were based on PCR amplification and sequencing of 16 S rRNA gene sequences.

    Quantitative RT-PCR:

    Article Title: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes
    Article Snippet: .. The obtained cDNA was used in qRT-PCR, which was performed in a 20-μL reaction mixture that contained one unit of Taq polymerase (Fermentas, Canada), 3.5 mM of MgCl2 , 1 unit of PCR buffer, 0.5 mM of each primer, 10 mM dNTPs, 0.5 units of SYBR Green I, 0.6 mL of DMSO (Sigma-Aldrich), and 2 μL of template cDNA, using a Roter-Gene Real-Time thermocycler (QIAGEN, USA). .. The PCR conditions were as follows: 95 °C for 1 min, followed by 40 cycles of 95 °C for 15 s, 58–60 °C for 30 s and 72 °C for 30 s. The relative expression of the cold-responsive genes (CBF3 and COR47) was calculated using the 2−ΔΔCt comparative CT method (Livak and Schmittgen ).

    Expressing:

    Article Title: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes
    Article Snippet: The obtained cDNA was used in qRT-PCR, which was performed in a 20-μL reaction mixture that contained one unit of Taq polymerase (Fermentas, Canada), 3.5 mM of MgCl2 , 1 unit of PCR buffer, 0.5 mM of each primer, 10 mM dNTPs, 0.5 units of SYBR Green I, 0.6 mL of DMSO (Sigma-Aldrich), and 2 μL of template cDNA, using a Roter-Gene Real-Time thermocycler (QIAGEN, USA). .. The PCR conditions were as follows: 95 °C for 1 min, followed by 40 cycles of 95 °C for 15 s, 58–60 °C for 30 s and 72 °C for 30 s. The relative expression of the cold-responsive genes (CBF3 and COR47) was calculated using the 2−ΔΔCt comparative CT method (Livak and Schmittgen ).

    Staining:

    Article Title: Bacterial release from pipe biofilm in a full-scale drinking water distribution system
    Article Snippet: .. Briefly, water samples in triplicate were stained with 5 µL mL−1 of SYBR Green I at 100× diluted with dimethyl sulphoxide (stock concentration 10,000 × , Invitrogen AG, Switzerland) at room temperature to a final concentration of 1× SYBR Green I and incubated at 37 °C for 15 min. ICC was determined by including 3 µM propidium iodide (1 mg mL−1 , Sigma-Aldrich, Germany). .. Results were exported as FCS files to FlowJo (Tree Star Inc, USA) and gated identically for all samples with green fluorescence (533 ± 30 nm) and red fluorescence ( > 670 nm).

    Article Title: Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci
    Article Snippet: .. After staining with 1 × SYBR Green I and 40 μg/mL propidium iodide, samples were incubated at room temperature for 25 min in the dark, harvested on GTBP filters (Ø = 0.2 μm, Millipore, Billerica, MA, USA), and examined under an epifluorescence microscope (Axioskop 2, Zeiss, Milano, Italy). .. CHECKERBOARD TEST Synergy was tested by the checkerboard method, a two-dimensional array of serial concentrations of test compounds, that has been used most frequently to assess antimicrobial combinations in vitro ( ).

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.
    Article Snippet: A volume of 50 μl of treated cells was stained overnight with SYBR Green I (10,000×) (Invitrogen, CA, USA) at 4°C. .. For the yeast cells, a concentration of SYBR Green I of 2% (vol/vol) was used from a 10-fold diluted working solution in Tris-EDTA (pH 8.0) (Sigma-Aldrich).

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Article Title: Mesoscale distribution and functional diversity of picoeukaryotes in the first-year sea ice of the Canadian Arctic
    Article Snippet: .. They were thawed in a 30 °C water bath and stained with SYBR-Green I in tris-EDTA buffer (10 mℳTris, 1 mℳEDTA; Sigma) ( , ). .. After cooling to ambient temperature, subsamples were analyzed with a FACSort flow cytometer (Becton and Dickinson, San Jose, CA, USA) equipped with an air-cooled argon laser (15 mW).

    Article Title: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
    Article Snippet: .. At 0 h, 24 h, 48 h, 72 h, 96 h, and 120 h, 5 μl of the culture was stained in 95 μl of D-PBS (Gibco) supplemented with 2× Sybr Green I (Ozyme; stock = 10,000×) for 30 min at room temperature, diluted 20-fold in D-PBS (final volume = 200 μl), and the Sybr Green fluorescence measured in a Guava easyCyte Flow Cytometer (EMD Millipore). ..

    Sequencing:

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). .. Phylogenetic analyses of growth-positive cultures were based on PCR amplification and sequencing of 16 S rRNA gene sequences.

    Sonication:

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.
    Article Snippet: A. nidulans conidia, after a brief sonication, were treated at 50°C for 2 h with RNase A (0.50 mg/ml) (GRiSP) and for 2 h with proteinase K (1 mg/ml) (GRiSP). .. For the yeast cells, a concentration of SYBR Green I of 2% (vol/vol) was used from a 10-fold diluted working solution in Tris-EDTA (pH 8.0) (Sigma-Aldrich).

    Live Dead Assay:

    Article Title: Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci
    Article Snippet: Paragraph title: LIVE/DEAD ASSAY ... After staining with 1 × SYBR Green I and 40 μg/mL propidium iodide, samples were incubated at room temperature for 25 min in the dark, harvested on GTBP filters (Ø = 0.2 μm, Millipore, Billerica, MA, USA), and examined under an epifluorescence microscope (Axioskop 2, Zeiss, Milano, Italy).

    Epifluorescence Microscopy:

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: Based on the total prokaryotic numbers determined by counting DAPI (4′,6-diamidino-2-phenylinole)-stained cells using epifluorescence microscopy, water samples were diluted to 5 cells per ml in HTC media and dispensed into 48-well tissue culture plates (1 ml per well). .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore).

    Cell Counting:

    Article Title: The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures
    Article Snippet: .. After incubation at 15 °C for 4 weeks, 200 μl cultures from each well of the plates was stained with SYBR Green I (5 × final concentration) and examined by flow cytometry for cell counting (Guava easyCyte Plus, Millipore). ..

    Plasmid Preparation:

    Article Title: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
    Article Snippet: Flow cytometry To measure the growth rate of 3D7+empty vector or 3D7+PfAlba1-Ty1 parasites, a synchronous culture of ring stages was diluted to 0.2 % parasitemia in 200 μl RPMI complete medium at 4 % hematocrit. .. At 0 h, 24 h, 48 h, 72 h, 96 h, and 120 h, 5 μl of the culture was stained in 95 μl of D-PBS (Gibco) supplemented with 2× Sybr Green I (Ozyme; stock = 10,000×) for 30 min at room temperature, diluted 20-fold in D-PBS (final volume = 200 μl), and the Sybr Green fluorescence measured in a Guava easyCyte Flow Cytometer (EMD Millipore).

    Software:

    Article Title: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes
    Article Snippet: The obtained cDNA was used in qRT-PCR, which was performed in a 20-μL reaction mixture that contained one unit of Taq polymerase (Fermentas, Canada), 3.5 mM of MgCl2 , 1 unit of PCR buffer, 0.5 mM of each primer, 10 mM dNTPs, 0.5 units of SYBR Green I, 0.6 mL of DMSO (Sigma-Aldrich), and 2 μL of template cDNA, using a Roter-Gene Real-Time thermocycler (QIAGEN, USA). .. Differences in the CBF3 and COR47 transcriptional patterns in response to different ASA concentrations were analyzed using SPSS 16.0 software.

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    Millipore lightcycler 480 sybr green i master
    Lightcycler 480 Sybr Green I Master, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybr green i master - by Bioz Stars, 2020-02
    93/100 stars
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    97
    Millipore sybr green i
    Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i/product/Millipore
    Average 97 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2020-02
    97/100 stars
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    79
    Millipore sybr green i stained cells
    Sybr Green I Stained Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i stained cells/product/Millipore
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sybr green i stained cells - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

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