sybr green i  (Millipore)


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  • 99
    Name:
    SYBR Green I nucleic acid gel stain
    Description:

    Catalog Number:
    s9430
    Price:
    None
    Applications:
    A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.
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    Structured Review

    Millipore sybr green i
    SYBR Green I nucleic acid gel stain

    https://www.bioz.com/result/sybr green i/product/Millipore
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    sybr green i - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense"

    Article Title: Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense

    Journal: BMC Genomics

    doi: 10.1186/s12864-015-1978-2

    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a , amplification curves; b , melting curve analysis
    Figure Legend Snippet: Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a , amplification curves; b , melting curve analysis

    Techniques Used: Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification

    2) Product Images from "Ploidy Determination in the Pathogenic Fungus Sporothrix spp."

    Article Title: Ploidy Determination in the Pathogenic Fungus Sporothrix spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.00284

    SYBR Green I nuclear staining of Sporothrix spp. Fluorescence microscopy analysis of (A) S. brasiliensis ATCC MYA-4823, (B) S. schenckii ATCC MYA-4821, and (C) S. pallida MUM 17.04 stained with SYBR Green I reveals the presence of mononucleated yeast cells. Images (1,036 × 1,024 pixels) were acquired in a fluorescence microscope (DP71 Olympus) with an oil immersion objective (100×/1.4) and cropped using the cellSens imaging software (Olympus Life Science). Scale bar equals 3 μm.
    Figure Legend Snippet: SYBR Green I nuclear staining of Sporothrix spp. Fluorescence microscopy analysis of (A) S. brasiliensis ATCC MYA-4823, (B) S. schenckii ATCC MYA-4821, and (C) S. pallida MUM 17.04 stained with SYBR Green I reveals the presence of mononucleated yeast cells. Images (1,036 × 1,024 pixels) were acquired in a fluorescence microscope (DP71 Olympus) with an oil immersion objective (100×/1.4) and cropped using the cellSens imaging software (Olympus Life Science). Scale bar equals 3 μm.

    Techniques Used: SYBR Green Assay, Staining, Fluorescence, Microscopy, Imaging, Software

    3) Product Images from "One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells"

    Article Title: One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells

    Journal: ACS Synthetic Biology

    doi: 10.1021/acssynbio.9b00034

    Confocal fluorescence images showing the diverse possibilities for encapsulation and reconstitution into the GUVs produced by the shaking method. Free-standing GUVs with (A) reconstituted TAMARA-labeled α IIb β 3 integrin; (B) Cy3-labeled membrane-adhering cholesterol-tagged DNA; (C) 100 nM encapsulated pyranine; (D) SYBR Green I-stained mRNA; (E) multifluorescent polystyrene beads; (F) mitochondria isolated from HeLa cells and stained with MitoTracker Green; (G) GFP-labeled E. coli ; (H) GUVs; and (I) lipid budding and tube formation in osmotically deflated GUVs. Scale bars: 10 μm.
    Figure Legend Snippet: Confocal fluorescence images showing the diverse possibilities for encapsulation and reconstitution into the GUVs produced by the shaking method. Free-standing GUVs with (A) reconstituted TAMARA-labeled α IIb β 3 integrin; (B) Cy3-labeled membrane-adhering cholesterol-tagged DNA; (C) 100 nM encapsulated pyranine; (D) SYBR Green I-stained mRNA; (E) multifluorescent polystyrene beads; (F) mitochondria isolated from HeLa cells and stained with MitoTracker Green; (G) GFP-labeled E. coli ; (H) GUVs; and (I) lipid budding and tube formation in osmotically deflated GUVs. Scale bars: 10 μm.

    Techniques Used: Fluorescence, Produced, Labeling, SYBR Green Assay, Staining, Isolation

    4) Product Images from "A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis [W] [W] [OPEN]"

    Article Title: A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis [W] [W] [OPEN]

    Journal: The Plant Cell

    doi: 10.1105/tpc.114.129254

    DEK3 Interaction with SSC3 and Change of DNA Topology. (A) seedlings. Immunoprecipitated proteins were subjected to protein gel blot analyses using GFP antibodies. Ten percent of the inputs were used as positive controls (left panel). (B) DEK3 can change the superhelical density of DNA. Plasmid DNA was incubated with increasing amounts of GST-DEK3 in the presence of topoisomerase I. Purified DNA was analyzed by agarose gel electrophoresis and SYBR Green I staining. The positions of DNA form I (supercoiled) and II (relaxed, closed circular, and nicked DNA) are indicated. A DNA size marker is shown on the left (M).
    Figure Legend Snippet: DEK3 Interaction with SSC3 and Change of DNA Topology. (A) seedlings. Immunoprecipitated proteins were subjected to protein gel blot analyses using GFP antibodies. Ten percent of the inputs were used as positive controls (left panel). (B) DEK3 can change the superhelical density of DNA. Plasmid DNA was incubated with increasing amounts of GST-DEK3 in the presence of topoisomerase I. Purified DNA was analyzed by agarose gel electrophoresis and SYBR Green I staining. The positions of DNA form I (supercoiled) and II (relaxed, closed circular, and nicked DNA) are indicated. A DNA size marker is shown on the left (M).

    Techniques Used: Immunoprecipitation, Western Blot, Plasmid Preparation, Incubation, Purification, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Marker

    Related Articles

    Conjugation Assay:

    Article Title: Attenuated diphtheria toxin mediates siRNA delivery
    Article Snippet: .. Successful conjugation was confirmed by polyacrylamide gel electrophoresis analysis, and conjugation efficiency was quantified using ImageJ software (45% total yield; 80% recovery of DT, 55% siRNA conjugation efficiency). .. Gene knockdown assays—Treatment with a DT-siRNA conjugate aDT-siRNA was mixed with Opti-MEM for a final concentration of 50 nM and added to 24-well Primaria plates coated with PLO/laminin.

    Agarose Gel Electrophoresis:

    Article Title: A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis [W] [W] [OPEN]
    Article Snippet: .. After proteinase K (#03115887001; Roche) digestion, DNA was precipitated and analyzed on a 0.8% agarose gel in 0.5× TBE (45 mM Tris-borate and 1 mM EDTA) at 2 V/cm for 16 h. The gel was stained with SYBR Green I (#S9430; Sigma-Aldrich). ..

    SYBR Green Assay:

    Article Title: Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo
    Article Snippet: .. Immediately prior to analysis, the slides were stained with 90 μL of SYBR GREEN I (Sigma-Aldrich, S 9430). .. Comets were visualized and captured with the 40x objective lens of a Leica DMLB fluorescence microscope, attached to a CCD camera.

    Article Title: Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense
    Article Snippet: .. A 10 μl reaction mixture was prepared for each sample, with the following components: 1 μl of Taq buffer (including 20 mM MgCl2 ) supplied together with Ex Taq HS (Takara), 0.25 μM forward primer, 0.25 μM reverse primer, 0.2 mM dNTPs, 0.7 mg/ml BSA (NEB), 0.5 × SYBR Green I (Sigma S9430), 3 % DMSO, 0.25 U of ExTaq HS, and sterile distilled water. .. The cycling conditions were 2 min at 95 °C and 5 s at 98 °C, followed by 35 or 45 cycles of 10 s at 98 °C, 10 s at 64 °C and 40 s at 72 °C (single acquisition of fluorescence signals).

    Article Title: miR-148a-3p regulates alcoholic liver fibrosis through targeting ERBB3
    Article Snippet: .. A volume of 0.5 µ l forward primer (10 µ M), 0.5 µ l reverse primer (10 µ M), 4 µ l cDNA, 5 µ l SYBR Green I nucleic acid gel stain (cat. no. S9430; Sigma-Aldrich; Merck KGaA) were mixed into 10 µ l solution. .. The thermocycler conditions for the qPCR assay was set as follows: Pretreatment at 95°C for 1 min, followed by 40 cycles of 95°C for 30 sec, 58°C for 20 sec and 70°C for 20 sec.

    Article Title: A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis [W] [W] [OPEN]
    Article Snippet: .. After proteinase K (#03115887001; Roche) digestion, DNA was precipitated and analyzed on a 0.8% agarose gel in 0.5× TBE (45 mM Tris-borate and 1 mM EDTA) at 2 V/cm for 16 h. The gel was stained with SYBR Green I (#S9430; Sigma-Aldrich). ..

    Article Title: Monitoring differentiation of human embryonic stem cells using real-time PCR.
    Article Snippet: .. There is a general lack of rapid, sensitive, and quantitative methods for the detection of differentiating human embryonic stem cells (hESCs). .. There is a general lack of rapid, sensitive, and quantitative methods for the detection of differentiating human embryonic stem cells (hESCs).

    Article Title: Loop mediated isothermal amplification of 5.8S rDNA for specific detection of Tritrichomonas foetus
    Article Snippet: .. For visual inspection, 2 μL of 1/100 dilution of SYBR Green I (S9430, Sigma–Aldrich) were added to 25 μL LAMP reactions. ..

    Article Title: One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells
    Article Snippet: .. For image acquisition, released RNA containing GUVs were incubated with 0.5× SYBR Green I (Sigma-Aldrich S9430, Germany) for 30 min and imaged as previously described. .. Polybead Polystyrene Microspheres with a diameter of 1 μm were purchased from Polysciences, Inc. (North America) and added at a concentration of 108 beads/to the aqueous phase for GUV formation.

    Incubation:

    Article Title: One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells
    Article Snippet: .. For image acquisition, released RNA containing GUVs were incubated with 0.5× SYBR Green I (Sigma-Aldrich S9430, Germany) for 30 min and imaged as previously described. .. Polybead Polystyrene Microspheres with a diameter of 1 μm were purchased from Polysciences, Inc. (North America) and added at a concentration of 108 beads/to the aqueous phase for GUV formation.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Attenuated diphtheria toxin mediates siRNA delivery
    Article Snippet: .. Successful conjugation was confirmed by polyacrylamide gel electrophoresis analysis, and conjugation efficiency was quantified using ImageJ software (45% total yield; 80% recovery of DT, 55% siRNA conjugation efficiency). .. Gene knockdown assays—Treatment with a DT-siRNA conjugate aDT-siRNA was mixed with Opti-MEM for a final concentration of 50 nM and added to 24-well Primaria plates coated with PLO/laminin.

    Staining:

    Article Title: Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo
    Article Snippet: .. Immediately prior to analysis, the slides were stained with 90 μL of SYBR GREEN I (Sigma-Aldrich, S 9430). .. Comets were visualized and captured with the 40x objective lens of a Leica DMLB fluorescence microscope, attached to a CCD camera.

    Article Title: miR-148a-3p regulates alcoholic liver fibrosis through targeting ERBB3
    Article Snippet: .. A volume of 0.5 µ l forward primer (10 µ M), 0.5 µ l reverse primer (10 µ M), 4 µ l cDNA, 5 µ l SYBR Green I nucleic acid gel stain (cat. no. S9430; Sigma-Aldrich; Merck KGaA) were mixed into 10 µ l solution. .. The thermocycler conditions for the qPCR assay was set as follows: Pretreatment at 95°C for 1 min, followed by 40 cycles of 95°C for 30 sec, 58°C for 20 sec and 70°C for 20 sec.

    Article Title: A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis [W] [W] [OPEN]
    Article Snippet: .. After proteinase K (#03115887001; Roche) digestion, DNA was precipitated and analyzed on a 0.8% agarose gel in 0.5× TBE (45 mM Tris-borate and 1 mM EDTA) at 2 V/cm for 16 h. The gel was stained with SYBR Green I (#S9430; Sigma-Aldrich). ..

    Software:

    Article Title: Attenuated diphtheria toxin mediates siRNA delivery
    Article Snippet: .. Successful conjugation was confirmed by polyacrylamide gel electrophoresis analysis, and conjugation efficiency was quantified using ImageJ software (45% total yield; 80% recovery of DT, 55% siRNA conjugation efficiency). .. Gene knockdown assays—Treatment with a DT-siRNA conjugate aDT-siRNA was mixed with Opti-MEM for a final concentration of 50 nM and added to 24-well Primaria plates coated with PLO/laminin.

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  • 99
    Millipore polyacrylamide gel electrophoresis analysis
    siRNAs can be conjugated to a DT. ( A ) a DT was engineered to contain a free cysteine as a functional handle ( a DT-SH), protected by a SUMO tag and purified using a histidine (His) tag. ( B ) a DT was reacted with a PEG cross-linker containing both maleimide and DBCO functional groups to obtain DBCO-modified a DT ( a DT-DBCO). ( C ) The presence of the DBCO modification on a DT was confirmed by measuring the absorbance at 280 nm ( a DT) and 309 nm (DBCO). Curves shown are a DT before modification (blue) and after DBCO modification (red). A 280 , absorbance at 280 nm. ( D ) Azide-modified siRNA was reacted with the DBCO-functionalized a DT to obtain the a DT-siRNA conjugate. RT, room temperature. ( E ) Modification of the a DT with the siRNA was confirmed via <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> (PAGE) stained with Coomassie blue to localize the DT protein. Lane 1 shows the a DT-DBCO starting material ( M w , ~72,000), and lane 2 shows the a DT-siRNA conjugate ( M w , ~90,000) alone with some unreacted starting material. ( F ) Purification of the excess siRNA was confirmed via PAGE stained with GelRed to localize the siRNA. Lane 3 shows the a DT-siRNA conjugate along with unreacted siRNA; lane 4 shows the a DT-siRNA conjugate after nickel column purification, with only a small amount of excess siRNA left over.
    Polyacrylamide Gel Electrophoresis Analysis, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyacrylamide gel electrophoresis analysis/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyacrylamide gel electrophoresis analysis - by Bioz Stars, 2020-10
    99/100 stars
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    99
    Millipore lightcycler 480 sybr green i master
    siRNAs can be conjugated to a DT. ( A ) a DT was engineered to contain a free cysteine as a functional handle ( a DT-SH), protected by a SUMO tag and purified using a histidine (His) tag. ( B ) a DT was reacted with a PEG cross-linker containing both maleimide and DBCO functional groups to obtain DBCO-modified a DT ( a DT-DBCO). ( C ) The presence of the DBCO modification on a DT was confirmed by measuring the absorbance at 280 nm ( a DT) and 309 nm (DBCO). Curves shown are a DT before modification (blue) and after DBCO modification (red). A 280 , absorbance at 280 nm. ( D ) Azide-modified siRNA was reacted with the DBCO-functionalized a DT to obtain the a DT-siRNA conjugate. RT, room temperature. ( E ) Modification of the a DT with the siRNA was confirmed via <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> (PAGE) stained with Coomassie blue to localize the DT protein. Lane 1 shows the a DT-DBCO starting material ( M w , ~72,000), and lane 2 shows the a DT-siRNA conjugate ( M w , ~90,000) alone with some unreacted starting material. ( F ) Purification of the excess siRNA was confirmed via PAGE stained with GelRed to localize the siRNA. Lane 3 shows the a DT-siRNA conjugate along with unreacted siRNA; lane 4 shows the a DT-siRNA conjugate after nickel column purification, with only a small amount of excess siRNA left over.
    Lightcycler 480 Sybr Green I Master, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybr green i master - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    siRNAs can be conjugated to a DT. ( A ) a DT was engineered to contain a free cysteine as a functional handle ( a DT-SH), protected by a SUMO tag and purified using a histidine (His) tag. ( B ) a DT was reacted with a PEG cross-linker containing both maleimide and DBCO functional groups to obtain DBCO-modified a DT ( a DT-DBCO). ( C ) The presence of the DBCO modification on a DT was confirmed by measuring the absorbance at 280 nm ( a DT) and 309 nm (DBCO). Curves shown are a DT before modification (blue) and after DBCO modification (red). A 280 , absorbance at 280 nm. ( D ) Azide-modified siRNA was reacted with the DBCO-functionalized a DT to obtain the a DT-siRNA conjugate. RT, room temperature. ( E ) Modification of the a DT with the siRNA was confirmed via polyacrylamide gel electrophoresis (PAGE) stained with Coomassie blue to localize the DT protein. Lane 1 shows the a DT-DBCO starting material ( M w , ~72,000), and lane 2 shows the a DT-siRNA conjugate ( M w , ~90,000) alone with some unreacted starting material. ( F ) Purification of the excess siRNA was confirmed via PAGE stained with GelRed to localize the siRNA. Lane 3 shows the a DT-siRNA conjugate along with unreacted siRNA; lane 4 shows the a DT-siRNA conjugate after nickel column purification, with only a small amount of excess siRNA left over.

    Journal: Science Advances

    Article Title: Attenuated diphtheria toxin mediates siRNA delivery

    doi: 10.1126/sciadv.aaz4848

    Figure Lengend Snippet: siRNAs can be conjugated to a DT. ( A ) a DT was engineered to contain a free cysteine as a functional handle ( a DT-SH), protected by a SUMO tag and purified using a histidine (His) tag. ( B ) a DT was reacted with a PEG cross-linker containing both maleimide and DBCO functional groups to obtain DBCO-modified a DT ( a DT-DBCO). ( C ) The presence of the DBCO modification on a DT was confirmed by measuring the absorbance at 280 nm ( a DT) and 309 nm (DBCO). Curves shown are a DT before modification (blue) and after DBCO modification (red). A 280 , absorbance at 280 nm. ( D ) Azide-modified siRNA was reacted with the DBCO-functionalized a DT to obtain the a DT-siRNA conjugate. RT, room temperature. ( E ) Modification of the a DT with the siRNA was confirmed via polyacrylamide gel electrophoresis (PAGE) stained with Coomassie blue to localize the DT protein. Lane 1 shows the a DT-DBCO starting material ( M w , ~72,000), and lane 2 shows the a DT-siRNA conjugate ( M w , ~90,000) alone with some unreacted starting material. ( F ) Purification of the excess siRNA was confirmed via PAGE stained with GelRed to localize the siRNA. Lane 3 shows the a DT-siRNA conjugate along with unreacted siRNA; lane 4 shows the a DT-siRNA conjugate after nickel column purification, with only a small amount of excess siRNA left over.

    Article Snippet: Successful conjugation was confirmed by polyacrylamide gel electrophoresis analysis, and conjugation efficiency was quantified using ImageJ software (45% total yield; 80% recovery of DT, 55% siRNA conjugation efficiency).

    Techniques: Functional Assay, Purification, Modification, Polyacrylamide Gel Electrophoresis, Staining, Nickel Column