swine kidney cell lines sk6  (ATCC)


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    ATCC swine kidney cell lines sk6
    Swine Kidney Cell Lines Sk6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/swine kidney cell lines sk6/product/ATCC
    Average 86 stars, based on 1 article reviews
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    swine kidney cell lines sk6 - by Bioz Stars, 2024-06
    86/100 stars

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    swine kidney cell lines sk6  (ATCC)


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    ATCC swine kidney cell lines sk6
    Treatment with the cGAS inhibitor G140 promotes FMDV infection. a IBRS-2 cells (2 × 10 5 ) were infected with FMDV O1K at an MOI of 0.01. One hour later, infection medium was replaced with fresh medium containing 20 µM G140 and cells were further incubated for 18 h. Then, supernatants were collected and cells were fixed for VP1 expression analysis (red) in comparison with non-treated cells under a wide field fluorescence microscope (left panel); scale bars, show 100 µm. Viral titers in the supernatants (tenfold dilutions) were quantified by plaque assay (right panel). b , c , d IBRS-2 ( b ), <t>SK6</t> ( c ) or WSL ( d ) cells were infected and treated with increasing concentrations of G140 as in ( a ) and 18 h later the supernatants were collected and viral titers were determined by plaque assay. Data are mean ± SD of at least three independent assays performed in duplicate. e HEK293T cells (0.4 × 10 6 ) were co-transfected with 25 ng of pIFN-β-FL, 12.5 ng of pRL-TK and either 2 ng of 2CARD or po cGAS/ h STING (10 ng each). After 7 h, G140 was added to the medium at the indicated concentrations and cells were incubated for 24 h. Then, cells were harvested for IFN-β assay. Data are mean ± SD of three independent assays. Significant differences (one-way ANOVA, p values) are indicated
    Swine Kidney Cell Lines Sk6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/swine kidney cell lines sk6/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    swine kidney cell lines sk6 - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "The antiviral response triggered by the cGAS/STING pathway is subverted by the foot-and-mouth disease virus proteases"

    Article Title: The antiviral response triggered by the cGAS/STING pathway is subverted by the foot-and-mouth disease virus proteases

    Journal: Cellular and Molecular Life Sciences

    doi: 10.1007/s00018-024-05190-7

    Treatment with the cGAS inhibitor G140 promotes FMDV infection. a IBRS-2 cells (2 × 10 5 ) were infected with FMDV O1K at an MOI of 0.01. One hour later, infection medium was replaced with fresh medium containing 20 µM G140 and cells were further incubated for 18 h. Then, supernatants were collected and cells were fixed for VP1 expression analysis (red) in comparison with non-treated cells under a wide field fluorescence microscope (left panel); scale bars, show 100 µm. Viral titers in the supernatants (tenfold dilutions) were quantified by plaque assay (right panel). b , c , d IBRS-2 ( b ), SK6 ( c ) or WSL ( d ) cells were infected and treated with increasing concentrations of G140 as in ( a ) and 18 h later the supernatants were collected and viral titers were determined by plaque assay. Data are mean ± SD of at least three independent assays performed in duplicate. e HEK293T cells (0.4 × 10 6 ) were co-transfected with 25 ng of pIFN-β-FL, 12.5 ng of pRL-TK and either 2 ng of 2CARD or po cGAS/ h STING (10 ng each). After 7 h, G140 was added to the medium at the indicated concentrations and cells were incubated for 24 h. Then, cells were harvested for IFN-β assay. Data are mean ± SD of three independent assays. Significant differences (one-way ANOVA, p values) are indicated
    Figure Legend Snippet: Treatment with the cGAS inhibitor G140 promotes FMDV infection. a IBRS-2 cells (2 × 10 5 ) were infected with FMDV O1K at an MOI of 0.01. One hour later, infection medium was replaced with fresh medium containing 20 µM G140 and cells were further incubated for 18 h. Then, supernatants were collected and cells were fixed for VP1 expression analysis (red) in comparison with non-treated cells under a wide field fluorescence microscope (left panel); scale bars, show 100 µm. Viral titers in the supernatants (tenfold dilutions) were quantified by plaque assay (right panel). b , c , d IBRS-2 ( b ), SK6 ( c ) or WSL ( d ) cells were infected and treated with increasing concentrations of G140 as in ( a ) and 18 h later the supernatants were collected and viral titers were determined by plaque assay. Data are mean ± SD of at least three independent assays performed in duplicate. e HEK293T cells (0.4 × 10 6 ) were co-transfected with 25 ng of pIFN-β-FL, 12.5 ng of pRL-TK and either 2 ng of 2CARD or po cGAS/ h STING (10 ng each). After 7 h, G140 was added to the medium at the indicated concentrations and cells were incubated for 24 h. Then, cells were harvested for IFN-β assay. Data are mean ± SD of three independent assays. Significant differences (one-way ANOVA, p values) are indicated

    Techniques Used: Infection, Incubation, Expressing, Comparison, Fluorescence, Microscopy, Plaque Assay, Transfection

    FMDV infection is enhanced by treatment with the gap-junction inhibitor carbenoxolone (CBX). IBRS-2 ( a ), SK6 ( b ) or WSL ( c ) cells (2 × 10 5 ) were treated with CBX at different concentrations for 3 h and then infected with FMDV O1K at an MOI of 0.01. Supernatants were collected 18 h after infection and viral titers were determined by plaque assay. Data are mean ± SD of three independent assays performed in duplicate. Significant differences (one-way ANOVA, p values) are indicated
    Figure Legend Snippet: FMDV infection is enhanced by treatment with the gap-junction inhibitor carbenoxolone (CBX). IBRS-2 ( a ), SK6 ( b ) or WSL ( c ) cells (2 × 10 5 ) were treated with CBX at different concentrations for 3 h and then infected with FMDV O1K at an MOI of 0.01. Supernatants were collected 18 h after infection and viral titers were determined by plaque assay. Data are mean ± SD of three independent assays performed in duplicate. Significant differences (one-way ANOVA, p values) are indicated

    Techniques Used: Infection, Plaque Assay

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    ATCC swine kidney cell lines sk6
    Swine Kidney Cell Lines Sk6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/swine kidney cell lines sk6/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    swine kidney cell lines sk6 - by Bioz Stars, 2024-06
    86/100 stars
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