Journal: STAR Protocols

Article Title: Protocol for applying expansion microscopy to the study of mammalian neuromuscular junctions

doi: 10.1016/j.xpro.2025.104272

Figure Lengend Snippet: Data analysis methods of expanded NMJs (A) Example of an expanded human NMJ stained with α-Bungarotoxin to label AChRs (cyan) and immunolabeled for SV2 to visualize presynaptic vesicles (magenta). Scale bar= 4 μm. A zoomed-in region (i) illustrates the selected area for measurement. AChR width was quantified on thresholded images (ii), indicated by red bars. AChR strip length and inter-strip spacing were measured on skeletonized images (iii), with yellow bars showing strip length and blue bars indicating spacing. Scale bar in (i)= 2 μm. (B) Mouse NMJ labeled as in (A). The yellow-dotted rectangle denotes the region used for fluorescence intensity profile analysis of AChRs and SV2. Intensity profiles were generated separately for each channel. Scale bar= 4 μm. Scale bar corresponds to the physical size of the expanded sample (not adjusted to expansion factor).

Article Snippet: Mouse anti-SV2 IgG antibody (1:50 dilution) , Developmental Studies Hybridoma Bank , Cat#2315387; RRID: AB-2315387.

Techniques: Staining, Immunolabeling, Stripping Membranes, Labeling, Fluorescence, Generated

Journal: The Journal of Physiological Sciences : JPS

Article Title: Activation of aortic baroreceptors depresses the somato–lumbar sympathetic reflex, reducing hindlimb muscle contractile force

doi: 10.1016/j.jphyss.2025.100051

Figure Lengend Snippet: Sympathetic nerves innervating an artery appear to connect to the neuromuscular junction (NMJ) in rat gastrocnemius muscle. A, B: Two representative confocal images showing sympathetic nerves (green, stained with anti-TH antibodies), arteries (magenta, visualized with α-elastin), and NMJs with motor nerves (blue, stained with anti-neurofilament and anti-SV2 antibodies) innervating muscle endplates (red, labeled with fluorescent α-bungarotoxin binding to acetylcholine receptors). a: Three-dimensional (3D) reconstructions spanning depths of 39–86 μm were generated from z-stack images acquired at 1-μm intervals and collapsed into maximum-intensity projections. Channel intensity and contrast were linearly adjusted to prepare RGB images. b, c: Enlarged view of the white boxed region in (a). The gray scale images in (c) present the TH signal. The right panel shows an orthogonal section along the vertical line indicated in the left panel.

Article Snippet: Anti-SV2 , 1:1000 , SV2 , AB_2315387 , DSHB , , .

Techniques: Staining, Labeling, Binding Assay, Generated