surveyor hplc system  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher surveyor hplc system
    Typical separation chromatograms of A) each trivalent As species from its corresponding pentavalent and GSH-complexed form obtained by <t>HPLC-ICP-MS.</t> B) GSH, GSSG and As-GSH complexes obtained by HPLC-ESI-MS. Single injection for a mixture of the standards
    Surveyor Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/surveyor hplc system/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    surveyor hplc system - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Speciation, formation, stability and analytical challenges of human arsenic metabolites"

    Article Title: Speciation, formation, stability and analytical challenges of human arsenic metabolites

    Journal: Journal of analytical atomic spectrometry

    doi: 10.1039/B910943A

    Typical separation chromatograms of A) each trivalent As species from its corresponding pentavalent and GSH-complexed form obtained by HPLC-ICP-MS. B) GSH, GSSG and As-GSH complexes obtained by HPLC-ESI-MS. Single injection for a mixture of the standards
    Figure Legend Snippet: Typical separation chromatograms of A) each trivalent As species from its corresponding pentavalent and GSH-complexed form obtained by HPLC-ICP-MS. B) GSH, GSSG and As-GSH complexes obtained by HPLC-ESI-MS. Single injection for a mixture of the standards

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Injection

    2) Product Images from "High-resolution high-performance liquid chromatography with electrospray ionization mass spectrometry and tandem mass spectrometry characterization of a new isoform of human salivary acidic proline-rich proteins named Roma-Boston Ser22(Phos) → Phe variant"

    Article Title: High-resolution high-performance liquid chromatography with electrospray ionization mass spectrometry and tandem mass spectrometry characterization of a new isoform of human salivary acidic proline-rich proteins named Roma-Boston Ser22(Phos) → Phe variant

    Journal: Journal of separation science

    doi: 10.1002/jssc.201400227

    Enlargement of the high-resolution HPLC–ESI-MS TIC profile of saliva from the three subjects showing the Roma-Boston variant, with the monoisotopic m / z values of the [M + H] 1+ ions and the position of the aPRPs isoforms detected. Panel A: Subject
    Figure Legend Snippet: Enlargement of the high-resolution HPLC–ESI-MS TIC profile of saliva from the three subjects showing the Roma-Boston variant, with the monoisotopic m / z values of the [M + H] 1+ ions and the position of the aPRPs isoforms detected. Panel A: Subject

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Variant Assay

    3) Product Images from "Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells"

    Article Title: Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0029906

    HPLC-DAD chromatograms from Aspergillus fumigatus (NRRL 35693) when grown in condition repressing (A) and inducing (B) conidiogenesis. The NRRL 35693 strain was cultured on Czapek-Glucose medium, at 25°C for 13 days in the dark under shaking (180 r.p.m.). Every day, the culture (mycelia and medium) was removed from the flask and placed in a new sterile flask in order to avoid that mycelium adhering to glass and sporulating in contact with the air. After 13 days, culture medium was harvested and analyzed by DAD-HPLC. The remaining pellets of mycelia were exposed to daylight without medium at 25°C in order to induce conidiogenesis. The secondary metabolites were extracted from pellets as described in the Materials and Methods.
    Figure Legend Snippet: HPLC-DAD chromatograms from Aspergillus fumigatus (NRRL 35693) when grown in condition repressing (A) and inducing (B) conidiogenesis. The NRRL 35693 strain was cultured on Czapek-Glucose medium, at 25°C for 13 days in the dark under shaking (180 r.p.m.). Every day, the culture (mycelia and medium) was removed from the flask and placed in a new sterile flask in order to avoid that mycelium adhering to glass and sporulating in contact with the air. After 13 days, culture medium was harvested and analyzed by DAD-HPLC. The remaining pellets of mycelia were exposed to daylight without medium at 25°C in order to induce conidiogenesis. The secondary metabolites were extracted from pellets as described in the Materials and Methods.

    Techniques Used: High Performance Liquid Chromatography, Cell Culture

    Representative HPLC-DAD and LC-MS chromatograms of conidial extract from A. fumigatus . (A) total scan photo diode array (PDA) chromatogram. Chromatogram of the ion at (B) m/z 403 in positive ionization mode (tryptoquivaline F), (C) m/z 444 in positive ionization mode (fumiquinazoline C), (D) m / z 345 in positive ionization mode (trypacidin), (E) m / z 345 in negative ionization mode (monomethylsulochrin), (F) m / z 283 in negative ionization mode (questin).
    Figure Legend Snippet: Representative HPLC-DAD and LC-MS chromatograms of conidial extract from A. fumigatus . (A) total scan photo diode array (PDA) chromatogram. Chromatogram of the ion at (B) m/z 403 in positive ionization mode (tryptoquivaline F), (C) m/z 444 in positive ionization mode (fumiquinazoline C), (D) m / z 345 in positive ionization mode (trypacidin), (E) m / z 345 in negative ionization mode (monomethylsulochrin), (F) m / z 283 in negative ionization mode (questin).

    Techniques Used: High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy

    4) Product Images from "Potent tumor targeting drug release system comprising MMP-2 specific peptide fragment with self-assembling characteristics"

    Article Title: Potent tumor targeting drug release system comprising MMP-2 specific peptide fragment with self-assembling characteristics

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/DDDT.S67305

    HPLC comparison of paclitaxel, SAMTA7, and the SAMTA7-paclitaxel combination mixed in different ratios. Notes: Shown are HPLC spectra for ( A ) paclitaxel, ( B ) SAMTA7, and the SAMTA7-paclitaxel combination at a ratio of ( C ) 1:1, ( D ) 1:5, and ( E ) 1:6. Samples were injected into an HPLC C18 column and detected at an ultraviolet wavelength of 214 nm. The retention time of paclitaxel and SAMTA7 was 29.6 minutes ( A ) and 5.4 minutes ( B ), respectively. A peak at 13.5 minutes was identified as an increased molar ratio of SAMTA7 and paclitaxel ( D ). At a molar ratio of 1:6, the peak of paclitaxel disappeared, while the appearance of a peak at 13.5 minutes indicated that SAMTA7 might interact with paclitaxel and form a stable complex ( E ). Abbreviation: HPLC, high-performance liquid chromatography.
    Figure Legend Snippet: HPLC comparison of paclitaxel, SAMTA7, and the SAMTA7-paclitaxel combination mixed in different ratios. Notes: Shown are HPLC spectra for ( A ) paclitaxel, ( B ) SAMTA7, and the SAMTA7-paclitaxel combination at a ratio of ( C ) 1:1, ( D ) 1:5, and ( E ) 1:6. Samples were injected into an HPLC C18 column and detected at an ultraviolet wavelength of 214 nm. The retention time of paclitaxel and SAMTA7 was 29.6 minutes ( A ) and 5.4 minutes ( B ), respectively. A peak at 13.5 minutes was identified as an increased molar ratio of SAMTA7 and paclitaxel ( D ). At a molar ratio of 1:6, the peak of paclitaxel disappeared, while the appearance of a peak at 13.5 minutes indicated that SAMTA7 might interact with paclitaxel and form a stable complex ( E ). Abbreviation: HPLC, high-performance liquid chromatography.

    Techniques Used: High Performance Liquid Chromatography, Injection

    5) Product Images from "The Surprising Composition of the Salivary Proteome of Preterm Human Newborn"

    Article Title: The Surprising Composition of the Salivary Proteome of Preterm Human Newborn

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1074/mcp.M110.003467

    Top-Down HPLC-ESI-MS Analysis of Whole Saliva of Preterm Newborns
    Figure Legend Snippet: Top-Down HPLC-ESI-MS Analysis of Whole Saliva of Preterm Newborns

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    Top-Down HPLC-ESI-MS Analysis of Whole Saliva of Preterm Newborns
    Figure Legend Snippet: Top-Down HPLC-ESI-MS Analysis of Whole Saliva of Preterm Newborns

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    Comparison between the total ion current HPLC-ESI-MS profiles of the acidic soluble fraction of whole saliva from an adult (34 years, panel A ) and a preterm newborn (226 days of postconceptional age, Panel B ). In the adult profile, the elution ranges
    Figure Legend Snippet: Comparison between the total ion current HPLC-ESI-MS profiles of the acidic soluble fraction of whole saliva from an adult (34 years, panel A ) and a preterm newborn (226 days of postconceptional age, Panel B ). In the adult profile, the elution ranges

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    Top-Down HPLC-ESI-MS Analysis of Whole Saliva of Preterm Newborns
    Figure Legend Snippet: Top-Down HPLC-ESI-MS Analysis of Whole Saliva of Preterm Newborns

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    6) Product Images from "Alterations of the Salivary Secretory Peptidome Profile in Children Affected by Type 1 Diabetes"

    Article Title: Alterations of the Salivary Secretory Peptidome Profile in Children Affected by Type 1 Diabetes

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1074/mcp.M110.001057

    Typical RP-HPLC-ESI profile of the acidic soluble fraction of human whole saliva of a control subject ( A ) and a Type 1 diabetic patient ( B ). Elution ranges of the principal classes of human salivary proteins/peptides are indicated. The other panels show
    Figure Legend Snippet: Typical RP-HPLC-ESI profile of the acidic soluble fraction of human whole saliva of a control subject ( A ) and a Type 1 diabetic patient ( B ). Elution ranges of the principal classes of human salivary proteins/peptides are indicated. The other panels show

    Techniques Used: High Performance Liquid Chromatography

    7) Product Images from "Dimethylarsinothioyl Glutathione as a Metabolite in Human Multiple Myeloma Cell Lines upon Exposure to Darinaparsin"

    Article Title: Dimethylarsinothioyl Glutathione as a Metabolite in Human Multiple Myeloma Cell Lines upon Exposure to Darinaparsin

    Journal: Chemical Research in Toxicology

    doi: 10.1021/tx400386c

    HPLC-ICP-MS chromatograms of the cell line and culture media both treated with DMA III (GS) to determine if the unknown As species (later identified as DMMTA V (GS)) can be produced by the culture media without the cells present. The extraction prior to analysis was performed in water. The As species identified are as follows: 1, DMA V ; 2, unknown As species; 3, DMA III ; 4, DMA III (GS); and 5, IS (internal standard, As V , delivered during HPLC-ICP-MS analysis for quantitative analysis and quality control).
    Figure Legend Snippet: HPLC-ICP-MS chromatograms of the cell line and culture media both treated with DMA III (GS) to determine if the unknown As species (later identified as DMMTA V (GS)) can be produced by the culture media without the cells present. The extraction prior to analysis was performed in water. The As species identified are as follows: 1, DMA V ; 2, unknown As species; 3, DMA III ; 4, DMA III (GS); and 5, IS (internal standard, As V , delivered during HPLC-ICP-MS analysis for quantitative analysis and quality control).

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Produced

    8) Product Images from "Identification of Three New N-Demethylated and O-Demethyled Bisbenzylisoquinoline Alkaloid Metabolites of Isoliensinine from Dog Hepatic Microsomes"

    Article Title: Identification of Three New N-Demethylated and O-Demethyled Bisbenzylisoquinoline Alkaloid Metabolites of Isoliensinine from Dog Hepatic Microsomes

    Journal: Molecules

    doi: 10.3390/molecules171011712

    HPLC-UV-ESI-MS profiles of the microsomal metabolites of isoliensine. The UV chromatogram was recorded at 280 nm, and selected ion chromatograms were pre-set at m/z 583 and 597 using data-dependent mode. Pink line shows the chromatogram of the control sample in the absence of NADPH.
    Figure Legend Snippet: HPLC-UV-ESI-MS profiles of the microsomal metabolites of isoliensine. The UV chromatogram was recorded at 280 nm, and selected ion chromatograms were pre-set at m/z 583 and 597 using data-dependent mode. Pink line shows the chromatogram of the control sample in the absence of NADPH.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    9) Product Images from "High-Throughput Analysis of Protein/Peptide Complexes by Immunoprecipitation and Automated LC-MS/MS"

    Article Title: High-Throughput Analysis of Protein/Peptide Complexes by Immunoprecipitation and Automated LC-MS/MS

    Journal: Journal of Biomolecular Techniques : JBT

    doi:

    Plumbing for automated analysis of multiple peptide samples using the HPLC autosampler and divert valve of LCQ mass spectrometer. A: In the waste mode, flow split before the peptide trap was blocked at valve position 6, so that all sample and solvent flowed through the trap. Peptides were trapped and solvent diverted to waste. The mass detector was set to Off during peptide trapping. B: In the source mode, flow split before the trap was on and diverted most solvent to waste. Flow split after the trap was blocked at valve position 6 to allow 150 nL/min of solvent gradient (detailed in Materials and Methods) to flow through the peptide trap to elute peptides into C18 microcapillary column. Separated peptides were injected into the mass spectrometer for MS and MS/MS analysis. Backpressure was controlled with the length of peek tubing (0.0025″ ID) at valve position 4.
    Figure Legend Snippet: Plumbing for automated analysis of multiple peptide samples using the HPLC autosampler and divert valve of LCQ mass spectrometer. A: In the waste mode, flow split before the peptide trap was blocked at valve position 6, so that all sample and solvent flowed through the trap. Peptides were trapped and solvent diverted to waste. The mass detector was set to Off during peptide trapping. B: In the source mode, flow split before the trap was on and diverted most solvent to waste. Flow split after the trap was blocked at valve position 6 to allow 150 nL/min of solvent gradient (detailed in Materials and Methods) to flow through the peptide trap to elute peptides into C18 microcapillary column. Separated peptides were injected into the mass spectrometer for MS and MS/MS analysis. Backpressure was controlled with the length of peek tubing (0.0025″ ID) at valve position 4.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Flow Cytometry, Injection

    10) Product Images from "Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation"

    Article Title: Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0067999

    XIC peak of Tβ4 evidenced by HPLC-ESI-MS analysis of the cytosolic (CF) and nuclear fractions (NF) of HepG2 cells grown for 48 h. Enlargements of the chromatographic profiles in the range 17.4–21.1 min are reported. Tβ4 search in CF of the cells grown under starvation and normal conditions, panel A and panel B; Tβ4 search in NF of the cells grown under starvation and normal conditions, panel C and panel D; arrows indicate the absence of the Tβ4 in its chromatographic elution range.
    Figure Legend Snippet: XIC peak of Tβ4 evidenced by HPLC-ESI-MS analysis of the cytosolic (CF) and nuclear fractions (NF) of HepG2 cells grown for 48 h. Enlargements of the chromatographic profiles in the range 17.4–21.1 min are reported. Tβ4 search in CF of the cells grown under starvation and normal conditions, panel A and panel B; Tβ4 search in NF of the cells grown under starvation and normal conditions, panel C and panel D; arrows indicate the absence of the Tβ4 in its chromatographic elution range.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    11) Product Images from "Dose-Dependent Effects of L-Arginine on PROP Bitterness Intensity and Latency and Characteristics of the Chemical Interaction between PROP and L-Arginine"

    Article Title: Dose-Dependent Effects of L-Arginine on PROP Bitterness Intensity and Latency and Characteristics of the Chemical Interaction between PROP and L-Arginine

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0131104

    Relative concentration of free L-Arg in the PROP taster groups in unstimulated (resting) saliva. Mean values ± SEM of the extract ion current (XIC) peak areas of L-Arg amino acid determined by HPLC-ESI-IT-MS analysis. n = 51. *Significant difference from the other values ( p ≤0.024; Newman-Keuls test).
    Figure Legend Snippet: Relative concentration of free L-Arg in the PROP taster groups in unstimulated (resting) saliva. Mean values ± SEM of the extract ion current (XIC) peak areas of L-Arg amino acid determined by HPLC-ESI-IT-MS analysis. n = 51. *Significant difference from the other values ( p ≤0.024; Newman-Keuls test).

    Techniques Used: Concentration Assay, High Performance Liquid Chromatography, Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites
    Article Snippet: .. HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany). ..

    Article Title: Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis
    Article Snippet: .. The microtitre plate was removed from the Triversa Nanomate and placed in the autosampler of the HPLC system (Ultimate 3000; Dionex, Thermo Fisher Scientific, Loughborough, UK), which is coupled to a Thermo Fisher Orbitrap Velos ETD mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via the Triversa Nanomate. .. Five μL aliquots were injected onto a Pepmap 100, C18 100 μm nanoviper trap (Thermo Fisher Scientific, Loughborough UK) for online desalting.

    Article Title: Adenosine diphosphate restricts the protein remodeling activity of the Hsp104 chaperone to Hsp70 assisted disaggregation
    Article Snippet: .. ADP concentration measurements Samples of the GFP renaturation reaction mixture (containing 1 μM Hsp104 D484K and optionally 2 μM Ssa1 and 0.5 μM Ydj1) were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) according to ( ) on GBC 1150 HPLC Pump, Spectra System AS3000 autosampler, Thermo Finnigan Spectra System UV6000LP. .. The separation was performed on 50 x 4,6 mm HyperClone 3u BDS C18, 130 A column (Phenomenex).

    Article Title: Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells
    Article Snippet: .. HPLC-PDA Analysis For the HPLC analysis of the extracts, a Thermo Finnigan® HPLC-PDA System (P4000 Pump, AS3000 Autosampler, PDA Detector UV8000, ChromQuest™ 4.2 Software) and a Supelco® RP18 Discovery HS-C18 (250 mm, 4.6 mm, and 5 μ m) column were employed. ..

    Article Title: Polyamines in biological samples: Rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography-tandem mass spectrometry
    Article Snippet: .. 2.4 Chromatographic methods All experiments were carried out on an Ultimate 3000 HPLC system comprising an autosampler and a 10-way switching valve unit coupled to a triple-quadrupole mass spectrometer, a Quantum TSQ Ultra AM, both from Thermo Fisher Scientific (San Jose, CA). .. The system was controlled by Xcalibur Software 2.1.

    Flow Cytometry:

    Article Title: A Genome-Wide Screen Identifies Factors Involved in S. aureus-Induced Human Neutrophil Cell Death and Pathogenesis
    Article Snippet: .. Plates were incubated at 37°C, 5% CO2 for 3 h. Following this, 100 μl cold PBS containing ToPro-3 (100 nM) was added to each well and samples were immediately subjected to flow cytometry using an Attune Autosampler (ThermoFisher, Waltham, MA). ..

    Cytometry:

    Article Title: A Genome-Wide Screen Identifies Factors Involved in S. aureus-Induced Human Neutrophil Cell Death and Pathogenesis
    Article Snippet: .. Plates were incubated at 37°C, 5% CO2 for 3 h. Following this, 100 μl cold PBS containing ToPro-3 (100 nM) was added to each well and samples were immediately subjected to flow cytometry using an Attune Autosampler (ThermoFisher, Waltham, MA). ..

    Purification:

    Article Title: Off-Line Multidimensional Liquid Chromatography and Auto Sampling Result in Sample Loss in LC/LC–MS/MS
    Article Snippet: .. Fractions were then loaded into a 2.5 RP resin column (offline MudPIT) or purified by stage tip and placed in autosampler vials (offline MudPIT with autosampler [EASY-nLC II, Thermo]). .. Both MudPIT and analytical columns were assembled using a zero-dead volume union (Upchurch Scientific, Oak Harbor, WA).

    Concentration Assay:

    Article Title: Adenosine diphosphate restricts the protein remodeling activity of the Hsp104 chaperone to Hsp70 assisted disaggregation
    Article Snippet: .. ADP concentration measurements Samples of the GFP renaturation reaction mixture (containing 1 μM Hsp104 D484K and optionally 2 μM Ssa1 and 0.5 μM Ydj1) were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) according to ( ) on GBC 1150 HPLC Pump, Spectra System AS3000 autosampler, Thermo Finnigan Spectra System UV6000LP. .. The separation was performed on 50 x 4,6 mm HyperClone 3u BDS C18, 130 A column (Phenomenex).

    Incubation:

    Article Title: A Genome-Wide Screen Identifies Factors Involved in S. aureus-Induced Human Neutrophil Cell Death and Pathogenesis
    Article Snippet: .. Plates were incubated at 37°C, 5% CO2 for 3 h. Following this, 100 μl cold PBS containing ToPro-3 (100 nM) was added to each well and samples were immediately subjected to flow cytometry using an Attune Autosampler (ThermoFisher, Waltham, MA). ..

    Mass Spectrometry:

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites
    Article Snippet: .. HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany). ..

    Article Title: Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis
    Article Snippet: .. The microtitre plate was removed from the Triversa Nanomate and placed in the autosampler of the HPLC system (Ultimate 3000; Dionex, Thermo Fisher Scientific, Loughborough, UK), which is coupled to a Thermo Fisher Orbitrap Velos ETD mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via the Triversa Nanomate. .. Five μL aliquots were injected onto a Pepmap 100, C18 100 μm nanoviper trap (Thermo Fisher Scientific, Loughborough UK) for online desalting.

    Article Title: Polyamines in biological samples: Rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography-tandem mass spectrometry
    Article Snippet: .. 2.4 Chromatographic methods All experiments were carried out on an Ultimate 3000 HPLC system comprising an autosampler and a 10-way switching valve unit coupled to a triple-quadrupole mass spectrometer, a Quantum TSQ Ultra AM, both from Thermo Fisher Scientific (San Jose, CA). .. The system was controlled by Xcalibur Software 2.1.

    Software:

    Article Title: Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells
    Article Snippet: .. HPLC-PDA Analysis For the HPLC analysis of the extracts, a Thermo Finnigan® HPLC-PDA System (P4000 Pump, AS3000 Autosampler, PDA Detector UV8000, ChromQuest™ 4.2 Software) and a Supelco® RP18 Discovery HS-C18 (250 mm, 4.6 mm, and 5 μ m) column were employed. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Thermo Fisher surveyor hplc system
    Enlargement of the high-resolution <t>HPLC–ESI-MS</t> TIC profile of saliva from the three subjects showing the Roma-Boston variant, with the monoisotopic m / z values of the [M + H] 1+ ions and the position of the aPRPs isoforms detected. Panel A: Subject
    Surveyor Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/surveyor hplc system/product/Thermo Fisher
    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    surveyor hplc system - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher surveyor hplc pump
    Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by <t>HPLC-MS/MS</t> quantification of <t>dG-C8-PhIP</t> in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p
    Surveyor Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/surveyor hplc pump/product/Thermo Fisher
    Average 88 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    surveyor hplc pump - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Enlargement of the high-resolution HPLC–ESI-MS TIC profile of saliva from the three subjects showing the Roma-Boston variant, with the monoisotopic m / z values of the [M + H] 1+ ions and the position of the aPRPs isoforms detected. Panel A: Subject

    Journal: Journal of separation science

    Article Title: High-resolution high-performance liquid chromatography with electrospray ionization mass spectrometry and tandem mass spectrometry characterization of a new isoform of human salivary acidic proline-rich proteins named Roma-Boston Ser22(Phos) → Phe variant

    doi: 10.1002/jssc.201400227

    Figure Lengend Snippet: Enlargement of the high-resolution HPLC–ESI-MS TIC profile of saliva from the three subjects showing the Roma-Boston variant, with the monoisotopic m / z values of the [M + H] 1+ ions and the position of the aPRPs isoforms detected. Panel A: Subject

    Article Snippet: The low-resolution HPLC–ESI-IT-MS apparatus was a Surveyor HPLC system (Thermo Fisher Scientific, San Jose, CA, USA) connected via a T splitter to a PDA diode-array detector and to an Advantage mass spectrometer.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Variant Assay

    Identification and purification of disulphide-bonded peptides from a tryptic digest of Pf332 DBL domain. A. Comparison of the analytical RP-HPLC elution profiles for DTT-reduced and non-reduced tryptic digest of refolded Pf332 DBL domain. Reduced (upper trace) and non-reduced (lower trace) digests (20 μg) were fractionated on a Vydac C18 (4.6 mm inner diameter × 250 mm) column under identical chromatographic conditions. The column was developed at a flow rate of 1 ml min −1 with a linear 120 min gradient from 0 to 70% buffer B, where buffer A was 0.05% (v/v) trifluoroacetic acid in Milli Q water, and buffer B was 0.05% (v/v) trifluoroacetic acid in acetonitrile. Asterisk-labelled peaks within the non-reduced chromatogram represent those altered by DTT reduction. B. Preparative scale RP-HPLC purification of peptides from the tryptic digest of Pf332 DBL domain. A total of 125 μg of digest was fractionated by elution from a Vydac C18 column (4.6 mm inner diameter × 250 mm) column using the elution conditions described in (A). Peptide fractions found to contain disulphide-bonded peptides by Edman degradation and mass spectrometry are indicated by peak numbers and correspond to those given in Table 1 (see Experimental procedures for further details).

    Journal: Molecular Microbiology

    Article Title: Analysis of structure and function of the giant protein Pf332 in Plasmodium falciparum

    doi: 10.1111/j.1365-2958.2008.06508.x

    Figure Lengend Snippet: Identification and purification of disulphide-bonded peptides from a tryptic digest of Pf332 DBL domain. A. Comparison of the analytical RP-HPLC elution profiles for DTT-reduced and non-reduced tryptic digest of refolded Pf332 DBL domain. Reduced (upper trace) and non-reduced (lower trace) digests (20 μg) were fractionated on a Vydac C18 (4.6 mm inner diameter × 250 mm) column under identical chromatographic conditions. The column was developed at a flow rate of 1 ml min −1 with a linear 120 min gradient from 0 to 70% buffer B, where buffer A was 0.05% (v/v) trifluoroacetic acid in Milli Q water, and buffer B was 0.05% (v/v) trifluoroacetic acid in acetonitrile. Asterisk-labelled peaks within the non-reduced chromatogram represent those altered by DTT reduction. B. Preparative scale RP-HPLC purification of peptides from the tryptic digest of Pf332 DBL domain. A total of 125 μg of digest was fractionated by elution from a Vydac C18 column (4.6 mm inner diameter × 250 mm) column using the elution conditions described in (A). Peptide fractions found to contain disulphide-bonded peptides by Edman degradation and mass spectrometry are indicated by peak numbers and correspond to those given in Table 1 (see Experimental procedures for further details).

    Article Snippet: The subdigest of tryptic fraction #35 was analysed after clean-up on C18 ZipTip (Millipore, Bedford, MA, USA) by RP-HPLC on a C18 column (3 μm, 0.15 mm inner diameter × 150 mm, Vydac) using a Surveyor-MS HPLC system (Thermo, San Jose, CA, USA).

    Techniques: Purification, High Performance Liquid Chromatography, Flow Cytometry, Mass Spectrometry

    HPLC comparison of paclitaxel, SAMTA7, and the SAMTA7-paclitaxel combination mixed in different ratios. Notes: Shown are HPLC spectra for ( A ) paclitaxel, ( B ) SAMTA7, and the SAMTA7-paclitaxel combination at a ratio of ( C ) 1:1, ( D ) 1:5, and ( E ) 1:6. Samples were injected into an HPLC C18 column and detected at an ultraviolet wavelength of 214 nm. The retention time of paclitaxel and SAMTA7 was 29.6 minutes ( A ) and 5.4 minutes ( B ), respectively. A peak at 13.5 minutes was identified as an increased molar ratio of SAMTA7 and paclitaxel ( D ). At a molar ratio of 1:6, the peak of paclitaxel disappeared, while the appearance of a peak at 13.5 minutes indicated that SAMTA7 might interact with paclitaxel and form a stable complex ( E ). Abbreviation: HPLC, high-performance liquid chromatography.

    Journal: Drug Design, Development and Therapy

    Article Title: Potent tumor targeting drug release system comprising MMP-2 specific peptide fragment with self-assembling characteristics

    doi: 10.2147/DDDT.S67305

    Figure Lengend Snippet: HPLC comparison of paclitaxel, SAMTA7, and the SAMTA7-paclitaxel combination mixed in different ratios. Notes: Shown are HPLC spectra for ( A ) paclitaxel, ( B ) SAMTA7, and the SAMTA7-paclitaxel combination at a ratio of ( C ) 1:1, ( D ) 1:5, and ( E ) 1:6. Samples were injected into an HPLC C18 column and detected at an ultraviolet wavelength of 214 nm. The retention time of paclitaxel and SAMTA7 was 29.6 minutes ( A ) and 5.4 minutes ( B ), respectively. A peak at 13.5 minutes was identified as an increased molar ratio of SAMTA7 and paclitaxel ( D ). At a molar ratio of 1:6, the peak of paclitaxel disappeared, while the appearance of a peak at 13.5 minutes indicated that SAMTA7 might interact with paclitaxel and form a stable complex ( E ). Abbreviation: HPLC, high-performance liquid chromatography.

    Article Snippet: Mixtures (20 μL) containing 10% dimethyl sulfoxide were analyzed using a Surveyor HPLC system with a C18 analytical column (Thermo Scientific, Waltham, MA, USA).

    Techniques: High Performance Liquid Chromatography, Injection

    Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-C8-PhIP in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p

    Journal: PLoS ONE

    Article Title: Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis

    doi: 10.1371/journal.pone.0058591

    Figure Lengend Snippet: Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-C8-PhIP in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p

    Article Snippet: The dG-C8-PhIP adducts were quantified by HPLC-MS/MS using of a Surveyor HPLC pump (Thermo Scientific, Les Ullis, France) associated with a TSQ Quantum Discovery Max triple quadrupole (Thermo Scientific, Les Ullis, France).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Synergic genotoxicity of B( a )P and PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of BaP and PhIP for 24 h. (A) Genotoxicity was evaluated by quantification by HPLC-MS/MS quantification of B( a )P- and PhIP-derived DNA adducts. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; **, p

    Journal: PLoS ONE

    Article Title: Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis

    doi: 10.1371/journal.pone.0058591

    Figure Lengend Snippet: Synergic genotoxicity of B( a )P and PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of BaP and PhIP for 24 h. (A) Genotoxicity was evaluated by quantification by HPLC-MS/MS quantification of B( a )P- and PhIP-derived DNA adducts. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; **, p

    Article Snippet: The dG-C8-PhIP adducts were quantified by HPLC-MS/MS using of a Surveyor HPLC pump (Thermo Scientific, Les Ullis, France) associated with a TSQ Quantum Discovery Max triple quadrupole (Thermo Scientific, Les Ullis, France).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay