superscriptii rnaseh  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscriptii rnaseh
    Superscriptii Rnaseh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptii rnaseh/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscriptii rnaseh - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Dominant Negative Mutation:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen). .. Dominant-negative (DN) TRPV2 was generated based on the short-form TRPV2/pEGFP-N2 ( ).

    Amplification:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen). .. The coding regions of mouse TRPV2 were PCR amplified from cDNA with the following 5′-EcoRI-mTRPV2 and 3′-XhoI-mTRPV2 primer sets: 5′-ATGGAATTCATGACTTCAGCCTCCAACCCCCCAGC-3′, and 5′-ATGCTCGAGTCAGTGGGACTGGAGGACCTGA-3′.

    Cell Culture:

    Article Title: Effects of Body Temperature on Neural Activity in the Hippocampus: Regulation of Resting Membrane Potentials by Transient Receptor Potential Vanilloid 4
    Article Snippet: Total RNA was prepared from various tissues of 8-week-old mice or cultured cells using Trizol reagent (Invitrogen). .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (−) Reverse Transcriptase (Invitrogen).

    Mouse Assay:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: Total RNA was prepared from the DRGs of adult ICR mice by using TRIzol reagent (Invitrogen). .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen).

    Article Title: Effects of Body Temperature on Neural Activity in the Hippocampus: Regulation of Resting Membrane Potentials by Transient Receptor Potential Vanilloid 4
    Article Snippet: Total RNA was prepared from various tissues of 8-week-old mice or cultured cells using Trizol reagent (Invitrogen). .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (−) Reverse Transcriptase (Invitrogen).

    Sequencing:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: The full coding sequence for murine TRPV2 was obtained by reverse transcription PCR. .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen).

    Generated:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen). .. Dominant-negative (DN) TRPV2 was generated based on the short-form TRPV2/pEGFP-N2 ( ).

    Expressing:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: All expression vector designs were based on the pCAGIG vector. .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: The full coding sequence for murine TRPV2 was obtained by reverse transcription PCR. .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen).

    Article Title: Effects of Body Temperature on Neural Activity in the Hippocampus: Regulation of Resting Membrane Potentials by Transient Receptor Potential Vanilloid 4
    Article Snippet: Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (−) Reverse Transcriptase (Invitrogen). .. Fragments of mouse TRPV4 or β- actin were PCR-amplified from the cDNA with primers (5′-mTRPV4 and 3′-mTRPV4 primer sets: 5′-CCGTGCACCAACATGAAGGTC-3′, 5′-TTGTTCAGCTCCACTACGCGG-3′), (5′-β-actin and 3′-β-actin primer sets: 5′-CAACGGCTCCGGCATGTGC-3′, 5′-CTCTTGCTCTGGGCCTCG-3′).

    Plasmid Preparation:

    Article Title: TRPV2 Enhances Axon Outgrowth through Its Activation by Membrane Stretch in Developing Sensory and Motor Neurons
    Article Snippet: The pCAGIG vector, which contains the IRES-EGFP cDNA under the control of the CMV enhancer and chick β-actin promoter, was a gift from Dr. C. Cepko [Harvard University, Cambridge, MA ( )]. .. Total RNA (1 μg) was converted to cDNA using SuperScriptII RNaseH (-) reverse transcriptase (Invitrogen).

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    Thermo Fisher rnase i
    Schematic workflow for glycosaminoglycan (GAG) extraction including RNase treatment Cell/tissue lysates are treated overnight with proteinase K (Prot. K), followed by DNase-I and <t>RNase-I</t> treatment and finally chloroform extraction and dialysis to remove contaminating proteins/DNA/RNA. After drying/concentration of GAG extracts, the purity of the preparations is assessed using ethidium bromide (EtBr) agarose gel electrophoresis, or by measuring the absorbance at 260 nm (A260).
    Rnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase i/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rnase i - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    98
    Thermo Fisher rnaseh
    Schematic workflow for glycosaminoglycan (GAG) extraction including RNase treatment Cell/tissue lysates are treated overnight with proteinase K (Prot. K), followed by DNase-I and <t>RNase-I</t> treatment and finally chloroform extraction and dialysis to remove contaminating proteins/DNA/RNA. After drying/concentration of GAG extracts, the purity of the preparations is assessed using ethidium bromide (EtBr) agarose gel electrophoresis, or by measuring the absorbance at 260 nm (A260).
    Rnaseh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseh/product/Thermo Fisher
    Average 98 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    rnaseh - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript iii rnase h reverse transcription
    Schematic workflow for glycosaminoglycan (GAG) extraction including RNase treatment Cell/tissue lysates are treated overnight with proteinase K (Prot. K), followed by DNase-I and <t>RNase-I</t> treatment and finally chloroform extraction and dialysis to remove contaminating proteins/DNA/RNA. After drying/concentration of GAG extracts, the purity of the preparations is assessed using ethidium bromide (EtBr) agarose gel electrophoresis, or by measuring the absorbance at 260 nm (A260).
    Superscript Iii Rnase H Reverse Transcription, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii rnase h reverse transcription/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii rnase h reverse transcription - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Schematic workflow for glycosaminoglycan (GAG) extraction including RNase treatment Cell/tissue lysates are treated overnight with proteinase K (Prot. K), followed by DNase-I and RNase-I treatment and finally chloroform extraction and dialysis to remove contaminating proteins/DNA/RNA. After drying/concentration of GAG extracts, the purity of the preparations is assessed using ethidium bromide (EtBr) agarose gel electrophoresis, or by measuring the absorbance at 260 nm (A260).

    Journal: PLoS ONE

    Article Title: RNA Contaminates Glycosaminoglycans Extracted from Cells and Tissues

    doi: 10.1371/journal.pone.0167336

    Figure Lengend Snippet: Schematic workflow for glycosaminoglycan (GAG) extraction including RNase treatment Cell/tissue lysates are treated overnight with proteinase K (Prot. K), followed by DNase-I and RNase-I treatment and finally chloroform extraction and dialysis to remove contaminating proteins/DNA/RNA. After drying/concentration of GAG extracts, the purity of the preparations is assessed using ethidium bromide (EtBr) agarose gel electrophoresis, or by measuring the absorbance at 260 nm (A260).

    Article Snippet: Adapted protocol: An excess of RNase-I (10 U/ml; Thermo Scientific) was added to the DNase mixture for subsequent extractions.

    Techniques: Concentration Assay, Agarose Gel Electrophoresis

    Glycosaminoglycans (GAG) extracts contain significant RNA impurities, which can be digested by RNase-I treatment. Visualizing nucleotide impurities in GAG preparations from conditionally immortalized mouse glomerular endothelial cells (mGEnC-1), human umbilical vein endothelial cells (HUVEC), immortalized retinal pigmental epithelial cells (ARPE-19) (A), and mouse kidney, heart, liver and spleen (B) on ethidium bromide agarose gels, revealed significant RNA contaminations. RNase-I treatment efficiently removed the contamination from mGEnC-1 and ARPE-19 GAGs, though minor impurities remained in HUVEC-, kidney- and spleen-derived extracts.

    Journal: PLoS ONE

    Article Title: RNA Contaminates Glycosaminoglycans Extracted from Cells and Tissues

    doi: 10.1371/journal.pone.0167336

    Figure Lengend Snippet: Glycosaminoglycans (GAG) extracts contain significant RNA impurities, which can be digested by RNase-I treatment. Visualizing nucleotide impurities in GAG preparations from conditionally immortalized mouse glomerular endothelial cells (mGEnC-1), human umbilical vein endothelial cells (HUVEC), immortalized retinal pigmental epithelial cells (ARPE-19) (A), and mouse kidney, heart, liver and spleen (B) on ethidium bromide agarose gels, revealed significant RNA contaminations. RNase-I treatment efficiently removed the contamination from mGEnC-1 and ARPE-19 GAGs, though minor impurities remained in HUVEC-, kidney- and spleen-derived extracts.

    Article Snippet: Adapted protocol: An excess of RNase-I (10 U/ml; Thermo Scientific) was added to the DNase mixture for subsequent extractions.

    Techniques: Derivative Assay

    RNA impurities interfere with the analysis of glycosaminoglycans (GAGs) using barium acetate agarose gel electrophoresis. Resolving untreated GAG extracts by barium acetate gel electrophoresis suggested relatively large amounts of heparan sulfate (HS) and dermatan sulfate (DS) and relatively little chondroitin sulfate (CS) in GAGs obtained from mouse glomerular endothelial cells (mGEnC-1), human umbilical vein endothelial cells (HUVEC) and immortalized retinal pigmental epithelial cells (ARPE-19) (A). GAG extracts from mouse tissues appeared to contain large amounts of DS (heart and liver) and CS (spleen), whereas kidney-derived GAGs were enriched in HS, but also contained DS and CS (B). However, the observed staining patterns seemed to result from contaminating RNA co-migrating between HS and DS, as RNase-I treatment revealed the actual GAG spots corresponding to primarily HS and CS.

    Journal: PLoS ONE

    Article Title: RNA Contaminates Glycosaminoglycans Extracted from Cells and Tissues

    doi: 10.1371/journal.pone.0167336

    Figure Lengend Snippet: RNA impurities interfere with the analysis of glycosaminoglycans (GAGs) using barium acetate agarose gel electrophoresis. Resolving untreated GAG extracts by barium acetate gel electrophoresis suggested relatively large amounts of heparan sulfate (HS) and dermatan sulfate (DS) and relatively little chondroitin sulfate (CS) in GAGs obtained from mouse glomerular endothelial cells (mGEnC-1), human umbilical vein endothelial cells (HUVEC) and immortalized retinal pigmental epithelial cells (ARPE-19) (A). GAG extracts from mouse tissues appeared to contain large amounts of DS (heart and liver) and CS (spleen), whereas kidney-derived GAGs were enriched in HS, but also contained DS and CS (B). However, the observed staining patterns seemed to result from contaminating RNA co-migrating between HS and DS, as RNase-I treatment revealed the actual GAG spots corresponding to primarily HS and CS.

    Article Snippet: Adapted protocol: An excess of RNase-I (10 U/ml; Thermo Scientific) was added to the DNase mixture for subsequent extractions.

    Techniques: Agarose Gel Electrophoresis, Nucleic Acid Electrophoresis, Derivative Assay, Staining