superscriptii revese transcriptase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher superscriptii revese transcriptase
    Superscriptii Revese Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptii revese transcriptase/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptii revese transcriptase - by Bioz Stars, 2020-04
    86/100 stars

    Related Products / Commonly Used Together

    strand cdna
    rna

    Images

    Related Articles

    RNA Extraction:

    Article Title: Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
    Article Snippet: Paragraph title: RNA Extraction and RT-PCR ... The first strand cDNA was synthesized from 0.5 ug of total RNA using SuperscriptII Revese Transcriptase (Invitrogen) and random hexamers.

    Amplification:

    Article Title: Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
    Article Snippet: The first strand cDNA was synthesized from 0.5 ug of total RNA using SuperscriptII Revese Transcriptase (Invitrogen) and random hexamers. .. The absence of contaminating genomic DNA was confirmed for each RNA extraction by PCR amplification of Gapdh specific product from RT negative samples.

    Synthesized:

    Article Title: Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
    Article Snippet: .. The first strand cDNA was synthesized from 0.5 ug of total RNA using SuperscriptII Revese Transcriptase (Invitrogen) and random hexamers. .. After synthesis, each cDNA was diluted 5-fold and 5 ul of diluted cDNA used in PCR reaction with gene-specific primers ( ).

    Isolation:

    Article Title: Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
    Article Snippet: RNA Extraction and RT-PCR Total RNA was extracted from 106 cells using High Pure RNA Isolation kit (Roche Diagnostics), with the inclusion of DNAseI treatment according to manufacturer’s instructions. .. The first strand cDNA was synthesized from 0.5 ug of total RNA using SuperscriptII Revese Transcriptase (Invitrogen) and random hexamers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
    Article Snippet: Paragraph title: RNA Extraction and RT-PCR ... The first strand cDNA was synthesized from 0.5 ug of total RNA using SuperscriptII Revese Transcriptase (Invitrogen) and random hexamers.

    Polymerase Chain Reaction:

    Article Title: Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
    Article Snippet: The first strand cDNA was synthesized from 0.5 ug of total RNA using SuperscriptII Revese Transcriptase (Invitrogen) and random hexamers. .. After synthesis, each cDNA was diluted 5-fold and 5 ul of diluted cDNA used in PCR reaction with gene-specific primers ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher superscript iii reverse transcriptase
    <t>RNA</t> populations present in cells infected with Ntail mutant viruses. (A and B) MiSeq analysis of viral P mRNA editing by recCDV NΔ441–479 (A), recMeV NΔ439–482 (B), or the corresponding parent virus. Values represent a minimum of 91,741 reads each and are expressed as mean percentage of the differentially edited mRNAs relative to total P ORF transcripts ± SEM. (C) qRT-PCR quantitation of relative CDV genome copy numbers in cells infected with the recCDV Ntail mutants. First-strand synthesis was done with specific primers binding to the viral genome untranslated region (UTR). (D and E) qRT-PCR quantitation of relative CDV N mRNA (D) and L mRNA (E) copy numbers present in RNA preparations as in panel C. First-strand synthesis was done with oligo(dT) primers. (F to H) qRT-PCR quantitations of RNA preparations as in panel C of the relative ratios of L and N protein-encoding mRNAs (F) and of polycistronic mRNAs covering the N/P (G) and mKate/L (H) intergenic sequence (IGSs). First-strand synthesis was done with oligo(dT) primers. In panels C to H, symbols represent individual values of <t>three</t> biological repeats analyzed in two technical replicates each. Columns show means ± SEM; one-way ANOVA with Tukey's post hoc test was performed.
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 3510 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    RNA populations present in cells infected with Ntail mutant viruses. (A and B) MiSeq analysis of viral P mRNA editing by recCDV NΔ441–479 (A), recMeV NΔ439–482 (B), or the corresponding parent virus. Values represent a minimum of 91,741 reads each and are expressed as mean percentage of the differentially edited mRNAs relative to total P ORF transcripts ± SEM. (C) qRT-PCR quantitation of relative CDV genome copy numbers in cells infected with the recCDV Ntail mutants. First-strand synthesis was done with specific primers binding to the viral genome untranslated region (UTR). (D and E) qRT-PCR quantitation of relative CDV N mRNA (D) and L mRNA (E) copy numbers present in RNA preparations as in panel C. First-strand synthesis was done with oligo(dT) primers. (F to H) qRT-PCR quantitations of RNA preparations as in panel C of the relative ratios of L and N protein-encoding mRNAs (F) and of polycistronic mRNAs covering the N/P (G) and mKate/L (H) intergenic sequence (IGSs). First-strand synthesis was done with oligo(dT) primers. In panels C to H, symbols represent individual values of three biological repeats analyzed in two technical replicates each. Columns show means ± SEM; one-way ANOVA with Tukey's post hoc test was performed.

    Journal: Journal of Virology

    Article Title: The Unstructured Paramyxovirus Nucleocapsid Protein Tail Domain Modulates Viral Pathogenesis through Regulation of Transcriptase Activity

    doi: 10.1128/JVI.02064-17

    Figure Lengend Snippet: RNA populations present in cells infected with Ntail mutant viruses. (A and B) MiSeq analysis of viral P mRNA editing by recCDV NΔ441–479 (A), recMeV NΔ439–482 (B), or the corresponding parent virus. Values represent a minimum of 91,741 reads each and are expressed as mean percentage of the differentially edited mRNAs relative to total P ORF transcripts ± SEM. (C) qRT-PCR quantitation of relative CDV genome copy numbers in cells infected with the recCDV Ntail mutants. First-strand synthesis was done with specific primers binding to the viral genome untranslated region (UTR). (D and E) qRT-PCR quantitation of relative CDV N mRNA (D) and L mRNA (E) copy numbers present in RNA preparations as in panel C. First-strand synthesis was done with oligo(dT) primers. (F to H) qRT-PCR quantitations of RNA preparations as in panel C of the relative ratios of L and N protein-encoding mRNAs (F) and of polycistronic mRNAs covering the N/P (G) and mKate/L (H) intergenic sequence (IGSs). First-strand synthesis was done with oligo(dT) primers. In panels C to H, symbols represent individual values of three biological repeats analyzed in two technical replicates each. Columns show means ± SEM; one-way ANOVA with Tukey's post hoc test was performed.

    Article Snippet: Briefly, 20 μl of extracted RNA was reverse transcribed using SuperScript III reverse transcriptase (Thermo), and second-strand synthesis was performed using Sequenase v2.0 (Agilent). cDNA was purified using DNA Clean and Concentrator-5 (Zymo) and subjected to Nextera XT tagmentation (Illumina) followed by 19 cycles of PCR amplification and a 0.8× Ampure XP cleanup (Beckman Coulter).

    Techniques: Infection, Mutagenesis, Quantitative RT-PCR, Quantitation Assay, Binding Assay, Sequencing

    Impact of MyD88 reactivation on the expression of factors that govern MO/DC function in infection and inflammation. (A, B) WT, MyD OFF , CD11c-MyD ON and LysM-MyD ON mice were infected orally with C . rodentium . On day 4 p.i. leukocytes were isolated from the cLP and sorted for DC (lin − CD64 − MHC-II + CD26 + CD11c + ) and MO (lin − CD64 + F4/80 + MHC-II + ). As lineage marker, antibodies against CD3, CD19, B220 and NK1.1 were included (A) Heat map displaying expression of genes involved in MNP function in infection and inflammation analyzed by real-time PCR from sorted DC/MO fractions. Data presented as mean log2 value of relative mRNA expression (see color scale). (B) Bar graphs show mean expression of selected genes in sorted DC/MO fractions relative to the expression of 5 housekeeping genes ( Actb , B2m , Gapdh , Gusb , Hsp90ab1 ). Data were pooled from three individual experiments with n = 4–6 mice per group. Error bar represents +SEM. One-Way ANOVA with Bonferroni’s Multiple Comparison test; *p

    Journal: PLoS Pathogens

    Article Title: MyD88 signaling in dendritic cells and the intestinal epithelium controls immunity against intestinal infection with C. rodentium

    doi: 10.1371/journal.ppat.1006357

    Figure Lengend Snippet: Impact of MyD88 reactivation on the expression of factors that govern MO/DC function in infection and inflammation. (A, B) WT, MyD OFF , CD11c-MyD ON and LysM-MyD ON mice were infected orally with C . rodentium . On day 4 p.i. leukocytes were isolated from the cLP and sorted for DC (lin − CD64 − MHC-II + CD26 + CD11c + ) and MO (lin − CD64 + F4/80 + MHC-II + ). As lineage marker, antibodies against CD3, CD19, B220 and NK1.1 were included (A) Heat map displaying expression of genes involved in MNP function in infection and inflammation analyzed by real-time PCR from sorted DC/MO fractions. Data presented as mean log2 value of relative mRNA expression (see color scale). (B) Bar graphs show mean expression of selected genes in sorted DC/MO fractions relative to the expression of 5 housekeeping genes ( Actb , B2m , Gapdh , Gusb , Hsp90ab1 ). Data were pooled from three individual experiments with n = 4–6 mice per group. Error bar represents +SEM. One-Way ANOVA with Bonferroni’s Multiple Comparison test; *p

    Article Snippet: 50–100 ng of amplified mRNA was transcribed into cDNA using SuperScript III Reverse Transcriptase Kit (Thermo Fisher Scientific).

    Techniques: Expressing, Infection, Mouse Assay, Isolation, Marker, Real-time Polymerase Chain Reaction

    Functional analysis of EBS mutants defective in H3K4me3 or H3K27me3 binding and a C-terminus deletion mutant. a , Plant phenotype of Col, ebs, ebs transformed with wild-type EBS (EBS-FLAG), H3K4 (EBS-FLAG Y155A) or H3K27 (EBS-FLAG Y49A W70L Y72A) binding-defective mutants, or EBSΔC (EBSΔC-FLAG). Scale bar, 1 cm. Images from three individual plants show similar results. b , Counting leaf number at flowering of long-day-grown plants described in a . The numbers 1 and 2 indicate two independent T 2 lines. Black horizontal lines represent the mean, and the error bars represent ±s.d. from the number of plants counted for each line. c , Relative FT mRNA levels after normalization to ACTIN7 in plants described in a . Data points are from three independent experiments (bars denote mean). d , Overlap of EBS- and EBSΔC-associated genes. Two biological replicates for ChIP-seq are shown as EBSΔC-Rep1 and EBSΔC-Rep2. The P value is based on the hypergeometric test. e , Box plot showing the average H3K4me3 levels at unique EBSΔC-associated peaks compared with peaks overlapping with EBS. Box edges show the IQR, horizontal center lines denote medians, and whiskers denote±1.5 IQR. f , Browser view of normalized ChIP-seq peaks at representative loci. g , Relative enrichment of EBS and EBSΔC at the indicated loci in f . h , i , Sequential ChIP–qPCR of H3K4me3 ( h ) or H3K27me3 ( i ) enrichment relative to EBS or EBSΔC input after initial EBS/EBSΔC ChIP ( TA3 is a negative-control locus). Bars denote the mean of two independent experiments.

    Journal: Nature genetics

    Article Title: EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis

    doi: 10.1038/s41588-018-0187-8

    Figure Lengend Snippet: Functional analysis of EBS mutants defective in H3K4me3 or H3K27me3 binding and a C-terminus deletion mutant. a , Plant phenotype of Col, ebs, ebs transformed with wild-type EBS (EBS-FLAG), H3K4 (EBS-FLAG Y155A) or H3K27 (EBS-FLAG Y49A W70L Y72A) binding-defective mutants, or EBSΔC (EBSΔC-FLAG). Scale bar, 1 cm. Images from three individual plants show similar results. b , Counting leaf number at flowering of long-day-grown plants described in a . The numbers 1 and 2 indicate two independent T 2 lines. Black horizontal lines represent the mean, and the error bars represent ±s.d. from the number of plants counted for each line. c , Relative FT mRNA levels after normalization to ACTIN7 in plants described in a . Data points are from three independent experiments (bars denote mean). d , Overlap of EBS- and EBSΔC-associated genes. Two biological replicates for ChIP-seq are shown as EBSΔC-Rep1 and EBSΔC-Rep2. The P value is based on the hypergeometric test. e , Box plot showing the average H3K4me3 levels at unique EBSΔC-associated peaks compared with peaks overlapping with EBS. Box edges show the IQR, horizontal center lines denote medians, and whiskers denote±1.5 IQR. f , Browser view of normalized ChIP-seq peaks at representative loci. g , Relative enrichment of EBS and EBSΔC at the indicated loci in f . h , i , Sequential ChIP–qPCR of H3K4me3 ( h ) or H3K27me3 ( i ) enrichment relative to EBS or EBSΔC input after initial EBS/EBSΔC ChIP ( TA3 is a negative-control locus). Bars denote the mean of two independent experiments.

    Article Snippet: One microgram of RNA was reverse-transcribed into cDNA with SuperScript III (Thermo Fisher, 18080093) followed by qPCR with SYBR Green Master Mix (Bio-Rad, 1725271) using CFX96 RealTime System 690 (Bio-Rad).

    Techniques: Functional Assay, Binding Assay, Mutagenesis, Transformation Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Negative Control

    Loss of shep strongly affects transcriptome of P14 pupal neurons. (A) Genome-wide fold changes of gene expression in response to loss of shep at third instar larval and P14 pupal stages. Dashed line indicates where gene expression is equally affected between the two stages. Note that the expression of dawdle is upregulated to more than 10-fold at both stages, which is out of plot range in both A and B. Genes inhibited by shep in pupae (red), larvae (yellow) and both (aqua) or promoted by shep in pupae (sky blue), larvae (green) or both (pink) are indicated. (B) The shep gene specifically regulates stage-biased genes during metamorphosis. Genes with (gray) or without (black) stage-biased expression during normal metamorphosis are indicated. Stage-biased genes that are shep- inhibited (red) or shep -promoted (blue) in pupae are indicated. Non-stage biased shep -regulated genes are indicated in purple. (C) The binary heat maps apply the same color scheme and summarize overlap between shep- regulated genes and genes with expression changes during normal metamorphosis. (D) Top GO terms of gene expression changes as a result of normal metamorphosis that are either larval-biased (gray) or pupal-biased (black) overlaid with changes due to loss of shep in pupal neurons that are shep -inhibited (red) or shep -promoted (blue). (E,F) Screenshots of RNA-seq signals in loss-of- shep P14 pupal neurons, and Shep, Su(Hw) and Mod(mdg4)67.2 ChIP-seq signals in BG3 cells at the Dad (E) and Myc (F) loci. (G) Validation of RNA-seq results by RT-qPCR in sorted pupal neurons. Three biological replicates for each genotype were analyzed with Student's t -test (* P

    Journal: Development (Cambridge, England)

    Article Title: Shep regulates Drosophila neuronal remodeling by controlling transcription of its chromatin targets

    doi: 10.1242/dev.154047

    Figure Lengend Snippet: Loss of shep strongly affects transcriptome of P14 pupal neurons. (A) Genome-wide fold changes of gene expression in response to loss of shep at third instar larval and P14 pupal stages. Dashed line indicates where gene expression is equally affected between the two stages. Note that the expression of dawdle is upregulated to more than 10-fold at both stages, which is out of plot range in both A and B. Genes inhibited by shep in pupae (red), larvae (yellow) and both (aqua) or promoted by shep in pupae (sky blue), larvae (green) or both (pink) are indicated. (B) The shep gene specifically regulates stage-biased genes during metamorphosis. Genes with (gray) or without (black) stage-biased expression during normal metamorphosis are indicated. Stage-biased genes that are shep- inhibited (red) or shep -promoted (blue) in pupae are indicated. Non-stage biased shep -regulated genes are indicated in purple. (C) The binary heat maps apply the same color scheme and summarize overlap between shep- regulated genes and genes with expression changes during normal metamorphosis. (D) Top GO terms of gene expression changes as a result of normal metamorphosis that are either larval-biased (gray) or pupal-biased (black) overlaid with changes due to loss of shep in pupal neurons that are shep -inhibited (red) or shep -promoted (blue). (E,F) Screenshots of RNA-seq signals in loss-of- shep P14 pupal neurons, and Shep, Su(Hw) and Mod(mdg4)67.2 ChIP-seq signals in BG3 cells at the Dad (E) and Myc (F) loci. (G) Validation of RNA-seq results by RT-qPCR in sorted pupal neurons. Three biological replicates for each genotype were analyzed with Student's t -test (* P

    Article Snippet: After washing, RNA captured on beads was re-suspended with 20 μl reaction system containing SuperScript III Reverse Transcriptase (Thermo Fisher Scientific), oligo (dT)20 and random primers for cDNA synthesis.

    Techniques: Genome Wide, Expressing, RNA Sequencing Assay, Chromatin Immunoprecipitation, Quantitative RT-PCR