Structured Review

TaKaRa superscriptii reverse transcriptase
Superscriptii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscriptii reverse transcriptase/product/TaKaRa
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
superscriptii reverse transcriptase - by Bioz Stars, 2020-04
91/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway
Article Snippet: Reverse transcription was performed using SuperscriptII reverse transcriptase (Takara). .. Quantitative real-time PCR (QPCR) was performed using SYBR Green I staining (Takara) on an iCycleri QTM system (BioRad) according to the manufacturer’s protocols.

Cell Culture:

Article Title: Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway
Article Snippet: Quantitative real-timePCR Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen) and DNA was removed by DNase (Promega). .. Reverse transcription was performed using SuperscriptII reverse transcriptase (Takara).

SYBR Green Assay:

Article Title: Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway
Article Snippet: Reverse transcription was performed using SuperscriptII reverse transcriptase (Takara). .. Quantitative real-time PCR (QPCR) was performed using SYBR Green I staining (Takara) on an iCycleri QTM system (BioRad) according to the manufacturer’s protocols.

Expressing:

Article Title: Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway
Article Snippet: Reverse transcription was performed using SuperscriptII reverse transcriptase (Takara). .. Gene expression levels were normalized to the internal GAPDH levels.

Polymerase Chain Reaction:

Article Title: Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway
Article Snippet: Reverse transcription was performed using SuperscriptII reverse transcriptase (Takara). .. The conditions were as follows: 38 cycles of three-step PCR (95°C for 40 s, 60°C for 50s, and 72°C for 30 s) after initial denaturation (95°C for 5 min).

Staining:

Article Title: Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway
Article Snippet: Reverse transcription was performed using SuperscriptII reverse transcriptase (Takara). .. Quantitative real-time PCR (QPCR) was performed using SYBR Green I staining (Takara) on an iCycleri QTM system (BioRad) according to the manufacturer’s protocols.

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  • 96
    TaKaRa superscript iii reverse transcriptase
    Validation by real-time RT-PCR of DNA <t>microarray</t> results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of <t>three</t> independent replicates. *, Significant difference ( P
    Superscript Iii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/TaKaRa
    Average 96 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    psti  (TaKaRa)
    99
    TaKaRa psti
    Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and <t>PstI</t> enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with <t>BamHI,</t> BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.
    Psti, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti/product/TaKaRa
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    psti - by Bioz Stars, 2020-04
    99/100 stars
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    93
    TaKaRa transcriptase superscript
    Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and <t>PstI</t> enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with <t>BamHI,</t> BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.
    Transcriptase Superscript, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptase superscript/product/TaKaRa
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    transcriptase superscript - by Bioz Stars, 2020-04
    93/100 stars
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    87
    TaKaRa transcriptase superscript ii kit
    Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and <t>PstI</t> enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with <t>BamHI,</t> BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.
    Transcriptase Superscript Ii Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptase superscript ii kit/product/TaKaRa
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transcriptase superscript ii kit - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

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    Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

    Journal: Journal of Virology

    Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿

    doi: 10.1128/JVI.02220-09

    Figure Lengend Snippet: Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

    Article Snippet: For validation of microarray data, total RNA was reverse transcribed into cDNAs using SuperScript III reverse transcriptase and random hexamer (TaKaRa Bio).

    Techniques: Quantitative RT-PCR, Microarray, Expressing

    Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and PstI enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with BamHI, BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.

    Journal: PLoS ONE

    Article Title: Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): A new European species of alphabaculovirus with a narrow host range

    doi: 10.1371/journal.pone.0176171

    Figure Lengend Snippet: Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and PstI enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with BamHI, BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.

    Article Snippet: For restriction endonuclease (REN) digestion, a sample of viral DNA (1–2 μg) was incubated with one of the following enzymes BamHI, BglII, EcoRI or PstI (Takara) (10 U) at 37°C for 4–12 h. Each reaction was stopped by the addition of 4 μl of 6x loading buffer (0.25% w/v bromophenol blue and 40% w/v sucrose).

    Techniques: