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Fisher Scientific superscriptii reverse transcriptase
Superscriptii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscriptii reverse transcriptase/product/Fisher Scientific
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
superscriptii reverse transcriptase - by Bioz Stars, 2020-04
93/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Genetic and transcriptomic analyses provide new insights on the early antiviral response to VHSV in resistant and susceptible rainbow trout
Article Snippet: .. RNA were reverse transcribed into cDNA using SuperscriptII reverse transcriptase (Fischer Scientific) and gene expression was assessed by qPCR using a CFX96 thermocycler (BioRad) following manufacturer’s instruction. ..

Amplification:

Article Title: Genetic and transcriptomic analyses provide new insights on the early antiviral response to VHSV in resistant and susceptible rainbow trout
Article Snippet: RNA were reverse transcribed into cDNA using SuperscriptII reverse transcriptase (Fischer Scientific) and gene expression was assessed by qPCR using a CFX96 thermocycler (BioRad) following manufacturer’s instruction. .. QPCR primers were designed using Primer3, and validated for target specificity and for an amplification efficiency > 80%.

Fluorescence In Situ Hybridization:

Article Title: Genetic and transcriptomic analyses provide new insights on the early antiviral response to VHSV in resistant and susceptible rainbow trout
Article Snippet: Paragraph title: Quantification of ISG expression in A22 fish ... RNA were reverse transcribed into cDNA using SuperscriptII reverse transcriptase (Fischer Scientific) and gene expression was assessed by qPCR using a CFX96 thermocycler (BioRad) following manufacturer’s instruction.

Expressing:

Article Title: Genetic and transcriptomic analyses provide new insights on the early antiviral response to VHSV in resistant and susceptible rainbow trout
Article Snippet: .. RNA were reverse transcribed into cDNA using SuperscriptII reverse transcriptase (Fischer Scientific) and gene expression was assessed by qPCR using a CFX96 thermocycler (BioRad) following manufacturer’s instruction. ..

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    Fisher Scientific superscript iii reverse transcriptase
    Alternative splicing of Arabidopsis HBS1/SKI7 has functional consequences. Arabidopsis HBS1/SKI7 ( At5g10630 ) is alternatively spliced in the nucleus, generating at least <t>three</t> distinct transcripts that are exported to the nucleus. The HBS1 transcript (left) putatitively encodes a translational GTPase, HBS1, that interacts with the decoding factor PELOTA to recognize stalled ribosomes. The SKI7 transcript (center) putatitively encodes SKI7, a protein that associates with the cytosolic <t>RNA</t> exosome, a 3′→5′ exoribonuclease complex that degrades aberrant transcripts. A third splice form (right) encodes a premature translation codon in the fourth exon, presumably triggering NMD after a pioneer round of translation.
    Superscript Iii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Fisher Scientific
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    96/100 stars
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    Alternative splicing of Arabidopsis HBS1/SKI7 has functional consequences. Arabidopsis HBS1/SKI7 ( At5g10630 ) is alternatively spliced in the nucleus, generating at least three distinct transcripts that are exported to the nucleus. The HBS1 transcript (left) putatitively encodes a translational GTPase, HBS1, that interacts with the decoding factor PELOTA to recognize stalled ribosomes. The SKI7 transcript (center) putatitively encodes SKI7, a protein that associates with the cytosolic RNA exosome, a 3′→5′ exoribonuclease complex that degrades aberrant transcripts. A third splice form (right) encodes a premature translation codon in the fourth exon, presumably triggering NMD after a pioneer round of translation.

    Journal: Frontiers in Plant Science

    Article Title: A Two-Headed Monster to Avert Disaster: HBS1/SKI7 Is Alternatively Spliced to Build Eukaryotic RNA Surveillance Complexes

    doi: 10.3389/fpls.2018.01333

    Figure Lengend Snippet: Alternative splicing of Arabidopsis HBS1/SKI7 has functional consequences. Arabidopsis HBS1/SKI7 ( At5g10630 ) is alternatively spliced in the nucleus, generating at least three distinct transcripts that are exported to the nucleus. The HBS1 transcript (left) putatitively encodes a translational GTPase, HBS1, that interacts with the decoding factor PELOTA to recognize stalled ribosomes. The SKI7 transcript (center) putatitively encodes SKI7, a protein that associates with the cytosolic RNA exosome, a 3′→5′ exoribonuclease complex that degrades aberrant transcripts. A third splice form (right) encodes a premature translation codon in the fourth exon, presumably triggering NMD after a pioneer round of translation.

    Article Snippet: RT-PCR and TOPO Cloning RNA was isolated from Ler Arabidopsis plants with the Spectrum Plant Total RNA (Sigma-Aldrich) kit with on-column DNase I digestion (New England Biolabs). cDNA was synthesized from RNA using oligo (dT)18 primers and SuperScript III reverse transcriptase (Fisher Scientific).

    Techniques: Functional Assay

    Arabidopsis HBS1/SKI7 is alternatively spliced. (A) The human HBS1/SKI7 locus, called HBS1L , produces three primary splice forms: HBS1L splice form 1, a long splice form with 18 exons; HBS1L splice form 2, a slightly shorter splice form skipping exon 3; and HBS1L splice form 3, which selects an alternative exon 5 followed by transcription termination. HBS1Lv3 includes sequences that promote interaction of the protein with the RNA exosome (yellow and red; see Figure 4A ). (B) RNA-Seq of light-grown seedlings shows that At5g10630 is alternatively spliced into three major splice forms ( Cheng et al., 2017 ). Cumulative RNA-Seq reads are shown in black (top panel), and select individual aligned reads are shown in green (bottom panel), with spliced sequences indicated by a black line. Note that reads for the fourth exon are ∼20% of the level of reads for the other coding sequence exons. (C) At5g10630 forms three major splice forms. A short splice form (top) skips exon 4 (yellow), yielding a transcript that encodes HBS1 (A) . A long splice form (middle) includes exon 4 (yellow), yielding a transcript that encodes SKI7 (A) . Rarely, an alternative acceptor site is selected for exon 4, adding five amino acids with no apparently functional consequence. A nonsense splice form (bottom) retains intron 4, which includes two codons, yielding a transcript that is likely subject to NMD. Exons are colored to match protein models in subsequent figures; UTRs are indicated with narrow, white bars.

    Journal: Frontiers in Plant Science

    Article Title: A Two-Headed Monster to Avert Disaster: HBS1/SKI7 Is Alternatively Spliced to Build Eukaryotic RNA Surveillance Complexes

    doi: 10.3389/fpls.2018.01333

    Figure Lengend Snippet: Arabidopsis HBS1/SKI7 is alternatively spliced. (A) The human HBS1/SKI7 locus, called HBS1L , produces three primary splice forms: HBS1L splice form 1, a long splice form with 18 exons; HBS1L splice form 2, a slightly shorter splice form skipping exon 3; and HBS1L splice form 3, which selects an alternative exon 5 followed by transcription termination. HBS1Lv3 includes sequences that promote interaction of the protein with the RNA exosome (yellow and red; see Figure 4A ). (B) RNA-Seq of light-grown seedlings shows that At5g10630 is alternatively spliced into three major splice forms ( Cheng et al., 2017 ). Cumulative RNA-Seq reads are shown in black (top panel), and select individual aligned reads are shown in green (bottom panel), with spliced sequences indicated by a black line. Note that reads for the fourth exon are ∼20% of the level of reads for the other coding sequence exons. (C) At5g10630 forms three major splice forms. A short splice form (top) skips exon 4 (yellow), yielding a transcript that encodes HBS1 (A) . A long splice form (middle) includes exon 4 (yellow), yielding a transcript that encodes SKI7 (A) . Rarely, an alternative acceptor site is selected for exon 4, adding five amino acids with no apparently functional consequence. A nonsense splice form (bottom) retains intron 4, which includes two codons, yielding a transcript that is likely subject to NMD. Exons are colored to match protein models in subsequent figures; UTRs are indicated with narrow, white bars.

    Article Snippet: RT-PCR and TOPO Cloning RNA was isolated from Ler Arabidopsis plants with the Spectrum Plant Total RNA (Sigma-Aldrich) kit with on-column DNase I digestion (New England Biolabs). cDNA was synthesized from RNA using oligo (dT)18 primers and SuperScript III reverse transcriptase (Fisher Scientific).

    Techniques: RNA Sequencing Assay, Sequencing, Functional Assay

    Alternative splicing of Arabidopsis HBS1/SKI7 has functional consequences. Arabidopsis HBS1/SKI7 ( At5g10630 ) is alternatively spliced in the nucleus, generating at least three distinct transcripts that are exported to the nucleus. The HBS1 transcript (left) putatitively encodes a translational GTPase, HBS1, that interacts with the decoding factor PELOTA to recognize stalled ribosomes. The SKI7 transcript (center) putatitively encodes SKI7, a protein that associates with the cytosolic RNA exosome, a 3′→5′ exoribonuclease complex that degrades aberrant transcripts. A third splice form (right) encodes a premature translation codon in the fourth exon, presumably triggering NMD after a pioneer round of translation.

    Journal: Frontiers in Plant Science

    Article Title: A Two-Headed Monster to Avert Disaster: HBS1/SKI7 Is Alternatively Spliced to Build Eukaryotic RNA Surveillance Complexes

    doi: 10.3389/fpls.2018.01333

    Figure Lengend Snippet: Alternative splicing of Arabidopsis HBS1/SKI7 has functional consequences. Arabidopsis HBS1/SKI7 ( At5g10630 ) is alternatively spliced in the nucleus, generating at least three distinct transcripts that are exported to the nucleus. The HBS1 transcript (left) putatitively encodes a translational GTPase, HBS1, that interacts with the decoding factor PELOTA to recognize stalled ribosomes. The SKI7 transcript (center) putatitively encodes SKI7, a protein that associates with the cytosolic RNA exosome, a 3′→5′ exoribonuclease complex that degrades aberrant transcripts. A third splice form (right) encodes a premature translation codon in the fourth exon, presumably triggering NMD after a pioneer round of translation.

    Article Snippet: RNA was isolated from L er Arabidopsis plants with the Spectrum Plant Total RNA (Sigma-Aldrich) kit with on-column DNase I digestion (New England Biolabs). cDNA was synthesized from RNA using oligo (dT)18 primers and SuperScript III reverse transcriptase (Fisher Scientific).

    Techniques: Functional Assay

    Depletion of RAD51 up-regulates innate immune response pathway genes. ( A ) shRNA-mediated depletion of RAD51 expression in HT1080 cells: HT1080 cells stably integrating tetracycline-inducible Rad51 shRNA were treated with 1 μg/ml doxycycline (DOX) for 72 h. Subsequently, cells were exposed to 1 Gy of radiation (IR), collected at pre-established time points after irradiation. Total cell lysates (50 μg) were separated on 8% SDS-PAGE and probed with anti-RAD51 and anti-Ku80 (loading control) antibodies. U- without DOX treatment. ( B ) Heat map of significantly altered innate immune response pathway genes in RAD51-proficient and -depleted cells 4 and 8 h after irradiation. ( C ) Graph shows fold changes in gene expression in irradiated (8 h) cells normalized to gene expression values in corresponding mock-treated cells. Exponentially growing RAD51-proficient and -depleted HT1080 cells were either mock-treated or irradiated with 1 Gy. Total RNA was prepared at indicated times after irradiation and analyzed for gene expression profiling using Human HT12v4 Arrays. The heat map for innate immune response network genes was generated with gene subsets created from the list of significant innate immune response genes using Spotfire Decision Site 9. (D–I) Differences in expression levels of innate immune response pathway genes measured by quantitative real-time polymerase chain reaction (qRT-PCR): RAD51-proficient and -depleted HT1080 cells were irradiated (IR) with 1 Gy and total RNA was prepared 8 h after irradiation. mRNAs were converted into cDNA and the levels of IL-6 ( D ), CSF2 ( E ), CXCR4 ( F ), TNF-α ( G ), CMKLR1 ( H ) and TLR9 ( I ) mRNA were quantified by qRT-PCR. Error bars represent the SEM from three independent experiments; * P

    Journal: Nucleic Acids Research

    Article Title: RAD51 interconnects between DNA replication, DNA repair and immunity

    doi: 10.1093/nar/gkx126

    Figure Lengend Snippet: Depletion of RAD51 up-regulates innate immune response pathway genes. ( A ) shRNA-mediated depletion of RAD51 expression in HT1080 cells: HT1080 cells stably integrating tetracycline-inducible Rad51 shRNA were treated with 1 μg/ml doxycycline (DOX) for 72 h. Subsequently, cells were exposed to 1 Gy of radiation (IR), collected at pre-established time points after irradiation. Total cell lysates (50 μg) were separated on 8% SDS-PAGE and probed with anti-RAD51 and anti-Ku80 (loading control) antibodies. U- without DOX treatment. ( B ) Heat map of significantly altered innate immune response pathway genes in RAD51-proficient and -depleted cells 4 and 8 h after irradiation. ( C ) Graph shows fold changes in gene expression in irradiated (8 h) cells normalized to gene expression values in corresponding mock-treated cells. Exponentially growing RAD51-proficient and -depleted HT1080 cells were either mock-treated or irradiated with 1 Gy. Total RNA was prepared at indicated times after irradiation and analyzed for gene expression profiling using Human HT12v4 Arrays. The heat map for innate immune response network genes was generated with gene subsets created from the list of significant innate immune response genes using Spotfire Decision Site 9. (D–I) Differences in expression levels of innate immune response pathway genes measured by quantitative real-time polymerase chain reaction (qRT-PCR): RAD51-proficient and -depleted HT1080 cells were irradiated (IR) with 1 Gy and total RNA was prepared 8 h after irradiation. mRNAs were converted into cDNA and the levels of IL-6 ( D ), CSF2 ( E ), CXCR4 ( F ), TNF-α ( G ), CMKLR1 ( H ) and TLR9 ( I ) mRNA were quantified by qRT-PCR. Error bars represent the SEM from three independent experiments; * P

    Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from 1 to 3 μg of RNA using SuperScript III Reverse Transcriptase (18-080-051 Fischer Scientific) in a total volume of 20 μl, according to manufacturer's instructions.

    Techniques: shRNA, Expressing, Stable Transfection, Irradiation, SDS Page, Generated, Real-time Polymerase Chain Reaction, Quantitative RT-PCR