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Bio-Rad superscriptii reverse transcriptase
Superscriptii Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscriptii reverse transcriptase/product/Bio-Rad
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
superscriptii reverse transcriptase - by Bioz Stars, 2020-04
91/100 stars

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Amplification:

Article Title: Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells
Article Snippet: For analysis of individual mRNAs, the RNA isolated from the IP was subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad). .. Amplification and quantification of the PCR products were performed using the Applied Biosystems Stepone Plus system (Applied Biosystems) and SYBR Green PCR Master Mix (SA Biosciences).

Isolation:

Article Title: RNA-Binding Protein FXR1 Regulates p21 and TERC RNA to Bypass p53-Mediated Cellular Senescence in OSCC
Article Snippet: .. For analysis of individual mRNAs, the RNA isolated from the IP is subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad). .. RNA extraction and qRT-PCR Total RNA is prepared from oral cancer tissues and HNSCC cell lines using Trizol (Ambion) or RNeasy mini kit (QIAGEN) by following manufacturer’s protocol. qRT-PCR for all m/RNA targets is performed using an Applied Biosystems StepOne Plus system with the SYBR green master mix RT-PCR kit (SA Biosciences).

Article Title: Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells
Article Snippet: .. For analysis of individual mRNAs, the RNA isolated from the IP was subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad). .. Amplification and quantification of the PCR products were performed using the Applied Biosystems Stepone Plus system (Applied Biosystems) and SYBR Green PCR Master Mix (SA Biosciences).

Quantitative RT-PCR:

Article Title: RNA-Binding Protein FXR1 Regulates p21 and TERC RNA to Bypass p53-Mediated Cellular Senescence in OSCC
Article Snippet: Paragraph title: FXR1 RNP IP (RIP) and qRT-PCR ... For analysis of individual mRNAs, the RNA isolated from the IP is subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad).

SYBR Green Assay:

Article Title: Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells
Article Snippet: For analysis of individual mRNAs, the RNA isolated from the IP was subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad). .. Amplification and quantification of the PCR products were performed using the Applied Biosystems Stepone Plus system (Applied Biosystems) and SYBR Green PCR Master Mix (SA Biosciences).

Incubation:

Article Title: RNA-Binding Protein FXR1 Regulates p21 and TERC RNA to Bypass p53-Mediated Cellular Senescence in OSCC
Article Snippet: For RNA analysis, the beads are incubated with 1ml NT2 buffer containing 20 U RNase-free DNase I (15 min, 30°C), washed twice with 1ml NT2 buffer and further incubated in 1 ml NT2 buffer containing 0.1% SDS and 0.5mg/ml proteinase K (15 min, 55°C) to digest the proteins bound to the beads. .. For analysis of individual mRNAs, the RNA isolated from the IP is subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad).

Article Title: Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells
Article Snippet: For RNA analysis, the beads were incubated with 1 ml NT2 buffer containing 20 U RNase-free DNase I (15 min, 30°C), washed twice with 1 ml NT2 buffer and further incubated in 1 ml NT2 buffer containing 0.1% SDS and 0.5 mg/ml proteinase K (15 min, 55°C) to digest the proteins bound to the beads. .. For analysis of individual mRNAs, the RNA isolated from the IP was subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad).

Polymerase Chain Reaction:

Article Title: Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells
Article Snippet: For analysis of individual mRNAs, the RNA isolated from the IP was subjected to reverse transcription (RT) using random hexamers and SuperScriptII reverse transcriptase (Biorad). .. Amplification and quantification of the PCR products were performed using the Applied Biosystems Stepone Plus system (Applied Biosystems) and SYBR Green PCR Master Mix (SA Biosciences).

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  • 94
    Bio-Rad superscript iii reverse transcriptase
    PPARs’ <t>mRNA</t> and protein expression during maturation and embryo development. ( A ) RT-PCR-based PPARα, PPARγ, and PPARδ expression at the COCs (cumulus oocyte complexes) (10 per sample), MII oocyte (20 per sample), two-cell embryo (20 per sample), 3.5-day embryo (10 per sample) and day-8 blastocyst stages (five per sample). ( B ) Relative mRNA expressions of PPARα, PPARγ and PPARδ from the COCs stage to day-8 blastocysts. ( C ) Development stage-dependent immunofluorescent expression of PPARα and PPARγ ( n = 15 per sample). ( D ) Immunofluorescent co-localization of PPARδ and PEPCK from COCs to day-8 blastocysts ( n = 15 per sample). The experiments were repeated <t>three</t> times, and the data are shown here as mean ± S.E.M. N.S., not significant. Significance: * p
    Superscript Iii Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Bio-Rad
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    95
    Bio-Rad superscript iii reverse transcriptase enzyme
    PPARs’ <t>mRNA</t> and protein expression during maturation and embryo development. ( A ) RT-PCR-based PPARα, PPARγ, and PPARδ expression at the COCs (cumulus oocyte complexes) (10 per sample), MII oocyte (20 per sample), two-cell embryo (20 per sample), 3.5-day embryo (10 per sample) and day-8 blastocyst stages (five per sample). ( B ) Relative mRNA expressions of PPARα, PPARγ and PPARδ from the COCs stage to day-8 blastocysts. ( C ) Development stage-dependent immunofluorescent expression of PPARα and PPARγ ( n = 15 per sample). ( D ) Immunofluorescent co-localization of PPARδ and PEPCK from COCs to day-8 blastocysts ( n = 15 per sample). The experiments were repeated <t>three</t> times, and the data are shown here as mean ± S.E.M. N.S., not significant. Significance: * p
    Superscript Iii Reverse Transcriptase Enzyme, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase enzyme/product/Bio-Rad
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase enzyme - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    PPARs’ mRNA and protein expression during maturation and embryo development. ( A ) RT-PCR-based PPARα, PPARγ, and PPARδ expression at the COCs (cumulus oocyte complexes) (10 per sample), MII oocyte (20 per sample), two-cell embryo (20 per sample), 3.5-day embryo (10 per sample) and day-8 blastocyst stages (five per sample). ( B ) Relative mRNA expressions of PPARα, PPARγ and PPARδ from the COCs stage to day-8 blastocysts. ( C ) Development stage-dependent immunofluorescent expression of PPARα and PPARγ ( n = 15 per sample). ( D ) Immunofluorescent co-localization of PPARδ and PEPCK from COCs to day-8 blastocysts ( n = 15 per sample). The experiments were repeated three times, and the data are shown here as mean ± S.E.M. N.S., not significant. Significance: * p

    Journal: International Journal of Molecular Sciences

    Article Title: The PPARδ Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos

    doi: 10.3390/ijms20236066

    Figure Lengend Snippet: PPARs’ mRNA and protein expression during maturation and embryo development. ( A ) RT-PCR-based PPARα, PPARγ, and PPARδ expression at the COCs (cumulus oocyte complexes) (10 per sample), MII oocyte (20 per sample), two-cell embryo (20 per sample), 3.5-day embryo (10 per sample) and day-8 blastocyst stages (five per sample). ( B ) Relative mRNA expressions of PPARα, PPARγ and PPARδ from the COCs stage to day-8 blastocysts. ( C ) Development stage-dependent immunofluorescent expression of PPARα and PPARγ ( n = 15 per sample). ( D ) Immunofluorescent co-localization of PPARδ and PEPCK from COCs to day-8 blastocysts ( n = 15 per sample). The experiments were repeated three times, and the data are shown here as mean ± S.E.M. N.S., not significant. Significance: * p

    Article Snippet: To denature the secondary structures, bound mRNAs were resuspended in 8 μL of 10 mM Tris-HCl and heated at 65 °C for 5 min, followed by rapid quenching of the reaction on ice for 3 min. Superscript III reverse transcriptase was used for mRNA to reverse-transcribe into first-strand cDNA (Bio-Rad Laboratories Hercules, CA, USA cat. #1708891).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    PPARδ reversibility and lipid metabolism in bovine embryos. ( A ) Immunofluorescent co-localization of PEPCK and CPT1 in control-, GSK3787-, and GW501516 IVC-treated day-8 blastocysts (15 per group). The original magnification is ×200. ( B ) RT-qPCR-based mRNA quantification of ATGL, LMF1, LMF2, and LPL in day-8 blastocysts (five per sample). ( C ) Western blot analysis of SIRT1 and p-mTOR in control-, GSK3787-, and GW501516 IVC-treated blastocysts (20 per group). The experiments were repeated three times, and the data are shown here as mean ± S.E.M. * p

    Journal: International Journal of Molecular Sciences

    Article Title: The PPARδ Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos

    doi: 10.3390/ijms20236066

    Figure Lengend Snippet: PPARδ reversibility and lipid metabolism in bovine embryos. ( A ) Immunofluorescent co-localization of PEPCK and CPT1 in control-, GSK3787-, and GW501516 IVC-treated day-8 blastocysts (15 per group). The original magnification is ×200. ( B ) RT-qPCR-based mRNA quantification of ATGL, LMF1, LMF2, and LPL in day-8 blastocysts (five per sample). ( C ) Western blot analysis of SIRT1 and p-mTOR in control-, GSK3787-, and GW501516 IVC-treated blastocysts (20 per group). The experiments were repeated three times, and the data are shown here as mean ± S.E.M. * p

    Article Snippet: To denature the secondary structures, bound mRNAs were resuspended in 8 μL of 10 mM Tris-HCl and heated at 65 °C for 5 min, followed by rapid quenching of the reaction on ice for 3 min. Superscript III reverse transcriptase was used for mRNA to reverse-transcribe into first-strand cDNA (Bio-Rad Laboratories Hercules, CA, USA cat. #1708891).

    Techniques: Quantitative RT-PCR, Western Blot