superscript kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher superscript kit
    Superscript Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript kit/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    superscript kit - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: Reverse transcription was performed with a Superscript kit (Invitrogen). .. For BMP immunodetection, 1 ml of spent-keratinocyte or control media were precipitated with two volumes of acetone, while keratinocytes were pelleted directly by centrifugation.

    Amplification:

    Article Title: The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection
    Article Snippet: RNA was reverse transcribed using the Superscript kit (Invitrogen, Carlsbad, CA) random hexamer protocol as per the manufacturer’s instructions. .. Amplification of gC1qR, cC1qR, C1qRp, and CD91 mRNA was performed using TaqMan® pre-designed primers from Applied Biosystems (Foster City, CA).

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy). .. Glyceraldehyde 3-phosphase dehydrogenase (GAPDH) cDNA was concurrently amplified as a housekeeping control.

    Synthesized:

    Article Title: Molecular Mechanisms of Classical Ehlers-Danlos Syndrome
    Article Snippet: .. Complementary DNA was synthesized from the RNA using the Superscript kit (Gibco BRL). .. Collagenous proteins were labeled and studied as previously described ( ).

    Quantitative RT-PCR:

    Article Title: Human iPSC-based Modeling of Late-Onset Disease via Progerin-induced Aging
    Article Snippet: RNA was extracted using the RNeasy kit (Qiagen) and reverse transcribed using the Superscript kit (Life Technologies) according to the manufacturer’s instructions. .. Quantitative RT–PCR was performed using the Mastercycler RealPlex2 (Eppendorf) platform.

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: Paragraph title: RNA Isolation and Semiquantitative and Quantitative RT-PCR Analysis ... RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy).

    Article Title: Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis
    Article Snippet: Paragraph title: QRT-PCR ... RT on WT, buc -/- and (−RT) control (from a WT RNA sample) sample was performed using random hexamer primers and the Superscript kit (Invitrogen), followed by RNAaseH reaction according to the kit instructions.

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: Phenotype of the BM-MSCs was determined by Real Time quantitative polymerase chain reaction (RT-qPCR). .. Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA).

    SYBR Green Assay:

    Article Title: Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis
    Article Snippet: RT on WT, buc -/- and (−RT) control (from a WT RNA sample) sample was performed using random hexamer primers and the Superscript kit (Invitrogen), followed by RNAaseH reaction according to the kit instructions. .. QPCR was performed using SYBR Green Jumpstart Taq Ready Mix (Sigma-Aldrich), in Step One Plus PCR machine (Applied Biosystems).

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA). .. All reactions were carried out in triplicate in a total volume of 10 μl with 2 μl of cDNA as template and Fast SYBR Green Master Mix (Applied Biosystems).

    Microarray:

    Article Title: Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair
    Article Snippet: Paragraph title: RNA preparation, cDNA synthesis, realtime PCR and microarray analysis ... Reverse transcription was performed with 1 µg of total RNA using the Superscript Kit (Invitrogen) and random nonamer primers.

    Random Hexamer Labeling:

    Article Title: The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection
    Article Snippet: .. RNA was reverse transcribed using the Superscript kit (Invitrogen, Carlsbad, CA) random hexamer protocol as per the manufacturer’s instructions. .. Amplification of gC1qR, cC1qR, C1qRp, and CD91 mRNA was performed using TaqMan® pre-designed primers from Applied Biosystems (Foster City, CA).

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer
    Article Snippet: .. The mRNA samples were extracted from mouse livers and colorectal specimen, as well as the human colorectal samples, and were DNAase-treated and reverse-transcribed using the SuperScript kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s recommendations using random hexamer primers. .. Q-PCR for (mouse and human) CD39, P2X7 and P2Y2 (Applied Biosystems, Foster City, CA, USA) was performed using the 7700 Sequence Detector (Applied Biosystems) and TaqMan technology as previously described [ ].

    Article Title: Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis
    Article Snippet: .. RT on WT, buc -/- and (−RT) control (from a WT RNA sample) sample was performed using random hexamer primers and the Superscript kit (Invitrogen), followed by RNAaseH reaction according to the kit instructions. .. QPCR was performed using SYBR Green Jumpstart Taq Ready Mix (Sigma-Aldrich), in Step One Plus PCR machine (Applied Biosystems).

    Expressing:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ]. .. Fold change expression was calculated using threshold cycle (C t ) values.

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen. .. Fold change expression was calculated using threshold cycle (Ct) values.

    Article Title: Human iPSC-based Modeling of Late-Onset Disease via Progerin-induced Aging
    Article Snippet: Paragraph title: Gene Expression Analysis ... RNA was extracted using the RNeasy kit (Qiagen) and reverse transcribed using the Superscript kit (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Skeletal mineralization deficits and impaired biogenesis and function of chondrocyte-derived matrix vesicles in Phospho1−/− and Phospho1/Pit1 double knockout mice
    Article Snippet: Paragraph title: Pi t1 gene expression ... For a 20 µl reaction, reverse transcription was carried out using the superscript kit (Life technologies, San Diego, CA) for 1 h at 42 °C followed by 70 °C for 5 min to inactivate the RT reagents.

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer
    Article Snippet: The mRNA samples were extracted from mouse livers and colorectal specimen, as well as the human colorectal samples, and were DNAase-treated and reverse-transcribed using the SuperScript kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s recommendations using random hexamer primers. .. Data were analyzed using the relative standard curve method with serial dilutions of cDNA derived from human fetal brain RNA (Clontech, Mountain View, CA, USA), and expression levels were normalized to the expression of 18S ribosomal subunit as internal controls.

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA). .. The expression levels of genes coding for MSC surface markers (CD90, CD105, CD73 and CD166), haematopoietic markers (CD34 and CD45) and immunogenic molecules (MHC-I and MHC-II) were analyzed using a StepOne Real Time PCR System device (Applied Biosystems) as described by Ranera et al. [ , ].

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: Paragraph title: BMP/Noggin expression analyses ... Reverse transcription was performed with a Superscript kit (Invitrogen).

    Derivative Assay:

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer
    Article Snippet: The mRNA samples were extracted from mouse livers and colorectal specimen, as well as the human colorectal samples, and were DNAase-treated and reverse-transcribed using the SuperScript kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s recommendations using random hexamer primers. .. Data were analyzed using the relative standard curve method with serial dilutions of cDNA derived from human fetal brain RNA (Clontech, Mountain View, CA, USA), and expression levels were normalized to the expression of 18S ribosomal subunit as internal controls.

    Transfection:

    Article Title: Cadherins mediate sequential roles through a hierarchy of mechanisms in the developing mammillary body
    Article Snippet: .. Forty eight hours after transfection RNA was extracted, treated with DNAse I and reverse transcribed with the Superscript kit (Invitrogen) (2 μg RNA per reaction). .. The RNA was quantitated by PCR (StepOne Plus, Applied Biosystems) using the neo transcript to normalize.

    Sequencing:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ]. .. Briefly, cDNA was generated by reverse transcription of 1 µg of total RNA using 100 nM of dNTPs, 50 µM of primer oligo dT15, 200 U of moloney murine leukaemia virus reverse transcriptase (M-MLV RT), 16 U of protector RNase inhibitor in RT buffer and 2.5 µL of 0.1 M DTT; real time PCR was performed using a StepOnePlus™ sequence detection system from Applied Biosystems (Darmstadt, Germany) with primer detecting TNF-α-sequence (fwd 5'-CAAACCACCAAGTGGAGGAG-3', rev 3'-GTGGGTGAGGAGCACGTAGT-5'), GAPDH (fwd 5'-AGAACATCATCCCTGCATCC-3', rev 3'-CACATTGGGGGTAGGAACAC-5') and β-actin (fwd 5'-ATTGTTACCAACTGGGACGACATG-3' rev 3'-CGAAGTCTAGAGCAACATAGCACA-5) as housekeeping genes.

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen. .. Briefly, cDNA was generated by reverse transcription of 1 μg of total RNA using 100 nM of dNTPs, 50 pM of primer oligo dT15, 200 U of moloney murine leukaemia virus reverse transcriptase (M-MLV RT), 16 U of protector RNase inhibitor in RT-buffer and 2.5 μl of 0.1 M DTT; real-time PCR was performed with a StepOnePlus– sequence detection system (Applied Biosystems, Darmstadt, Germany) as described previously with the primers shown in .

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer
    Article Snippet: The mRNA samples were extracted from mouse livers and colorectal specimen, as well as the human colorectal samples, and were DNAase-treated and reverse-transcribed using the SuperScript kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s recommendations using random hexamer primers. .. Q-PCR for (mouse and human) CD39, P2X7 and P2Y2 (Applied Biosystems, Foster City, CA, USA) was performed using the 7700 Sequence Detector (Applied Biosystems) and TaqMan technology as previously described [ ].

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy). .. Reactions were performed in an ABI PRISM 7700 Sequence Detection System (Applied Biosystems) using the following parameters: 40 cycles of a two-step PCR program at 95°C for 15 sec and 60°C for 60 sec, after an initial denaturation/activation step at 95°C for 10 min. For semiquantitative RT-PCR determinations, aliquots were harvested from the amplification reactions after 20, 25, 30, and 35 cycles and separated on agarose gel.

    Chromatography:

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: RNAs were purified by Trizol-extraction of keratinocytes (Invitrogen) and spin-column chromatography (Qiagen). .. Reverse transcription was performed with a Superscript kit (Invitrogen).

    Cell Culture:

    Article Title: Molecular Mechanisms of Classical Ehlers-Danlos Syndrome
    Article Snippet: Paragraph title: Cell Culture; Isolation of DNA and RNA; Protein Analysis ... Complementary DNA was synthesized from the RNA using the Superscript kit (Gibco BRL).

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: For chondrogenic differentiation, approximately 500.000 cells were pelleted in 15 mL conical tubes and cultured in chondrogenic medium for 21 days. .. Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA).

    Generated:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ]. .. Briefly, cDNA was generated by reverse transcription of 1 µg of total RNA using 100 nM of dNTPs, 50 µM of primer oligo dT15, 200 U of moloney murine leukaemia virus reverse transcriptase (M-MLV RT), 16 U of protector RNase inhibitor in RT buffer and 2.5 µL of 0.1 M DTT; real time PCR was performed using a StepOnePlus™ sequence detection system from Applied Biosystems (Darmstadt, Germany) with primer detecting TNF-α-sequence (fwd 5'-CAAACCACCAAGTGGAGGAG-3', rev 3'-GTGGGTGAGGAGCACGTAGT-5'), GAPDH (fwd 5'-AGAACATCATCCCTGCATCC-3', rev 3'-CACATTGGGGGTAGGAACAC-5') and β-actin (fwd 5'-ATTGTTACCAACTGGGACGACATG-3' rev 3'-CGAAGTCTAGAGCAACATAGCACA-5) as housekeeping genes.

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen. .. Briefly, cDNA was generated by reverse transcription of 1 μg of total RNA using 100 nM of dNTPs, 50 pM of primer oligo dT15, 200 U of moloney murine leukaemia virus reverse transcriptase (M-MLV RT), 16 U of protector RNase inhibitor in RT-buffer and 2.5 μl of 0.1 M DTT; real-time PCR was performed with a StepOnePlus– sequence detection system (Applied Biosystems, Darmstadt, Germany) as described previously with the primers shown in .

    Polymerase Chain Reaction:

    Article Title: Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair
    Article Snippet: Paragraph title: RNA preparation, cDNA synthesis, realtime PCR and microarray analysis ... Reverse transcription was performed with 1 µg of total RNA using the Superscript Kit (Invitrogen) and random nonamer primers.

    Article Title: The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection
    Article Snippet: Paragraph title: Polymerase chain reaction ... RNA was reverse transcribed using the Superscript kit (Invitrogen, Carlsbad, CA) random hexamer protocol as per the manufacturer’s instructions.

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer
    Article Snippet: The mRNA samples were extracted from mouse livers and colorectal specimen, as well as the human colorectal samples, and were DNAase-treated and reverse-transcribed using the SuperScript kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s recommendations using random hexamer primers. .. Reactions (of 25 μl) were set up using the 2× Universal PCR Master Mix (Applied Biosystems), template cDNA and adequate concentrations of primers and probes.

    Article Title: Cadherins mediate sequential roles through a hierarchy of mechanisms in the developing mammillary body
    Article Snippet: Forty eight hours after transfection RNA was extracted, treated with DNAse I and reverse transcribed with the Superscript kit (Invitrogen) (2 μg RNA per reaction). .. The RNA was quantitated by PCR (StepOne Plus, Applied Biosystems) using the neo transcript to normalize.

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy). .. Reactions were performed in an ABI PRISM 7700 Sequence Detection System (Applied Biosystems) using the following parameters: 40 cycles of a two-step PCR program at 95°C for 15 sec and 60°C for 60 sec, after an initial denaturation/activation step at 95°C for 10 min. For semiquantitative RT-PCR determinations, aliquots were harvested from the amplification reactions after 20, 25, 30, and 35 cycles and separated on agarose gel.

    Article Title: Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis
    Article Snippet: RT on WT, buc -/- and (−RT) control (from a WT RNA sample) sample was performed using random hexamer primers and the Superscript kit (Invitrogen), followed by RNAaseH reaction according to the kit instructions. .. QPCR was performed using SYBR Green Jumpstart Taq Ready Mix (Sigma-Aldrich), in Step One Plus PCR machine (Applied Biosystems).

    RNA Sequencing Assay:

    Article Title: Human iPSC-based Modeling of Late-Onset Disease via Progerin-induced Aging
    Article Snippet: RNA was extracted using the RNeasy kit (Qiagen) and reverse transcribed using the Superscript kit (Life Technologies) according to the manufacturer’s instructions. .. For RNA-seq, total RNA was isolated from two independent experiments and processed by the MSKCC Genomic core facility.

    Irradiation:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: RNA Isolation and Real-Time PCR Analysis Total RNA from the livers of irradiated and sham-irradiated mice was isolated after homogenization in Trizol® (Invitrogen) according to manufacturers’ protocol. .. For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ].

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: RNA isolation and real-time PCR analysis Total RNA from the livers of irradiated and sham-irradiated mice was isolated after homogenization in Trizol (Invitrogen) as described previously . .. For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen.

    Isolation:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: Paragraph title: 4.7. RNA Isolation and Real-Time PCR Analysis ... For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ].

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: Paragraph title: RNA isolation and real-time PCR analysis ... For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen.

    Article Title: Upregulation of cathepsin D in the caudate nucleus of primates with experimental parkinsonism
    Article Snippet: Paragraph title: RNA isolation and Quantitative Real Time PCR ... In a 50 μ L reaction, reverse transcription (RT) was carried out using the Superscript kit (Invitrogen) for 1 hr at 42°C, followed by 70°C for 5 min to inactivate the RT reagents.

    Article Title: Molecular Mechanisms of Classical Ehlers-Danlos Syndrome
    Article Snippet: Paragraph title: Cell Culture; Isolation of DNA and RNA; Protein Analysis ... Complementary DNA was synthesized from the RNA using the Superscript kit (Gibco BRL).

    Article Title: Human iPSC-based Modeling of Late-Onset Disease via Progerin-induced Aging
    Article Snippet: RNA was extracted using the RNeasy kit (Qiagen) and reverse transcribed using the Superscript kit (Life Technologies) according to the manufacturer’s instructions. .. For RNA-seq, total RNA was isolated from two independent experiments and processed by the MSKCC Genomic core facility.

    Article Title: Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair
    Article Snippet: RNA preparation, cDNA synthesis, realtime PCR and microarray analysis RNA was isolated from sciatic nerves using the RNeasy Kit (Qiagen) according to manufacturer’s instructions and the concentration and quality (ratio of absorption at 260/280 nm) evaluated using the NanoDrop spectrophotometer. .. Reverse transcription was performed with 1 µg of total RNA using the Superscript Kit (Invitrogen) and random nonamer primers.

    Article Title: Protective Role for the Disulfide Isomerase PDIA3 in Methamphetamine Neurotoxicity
    Article Snippet: Quantitative real-time PCR Isolated RNA was used for reverse transcription. .. In brief, reverse transcription was carried out using the Superscript kit (Life Technologies) at 2 µg RNA per 50 µl reaction, for 1 h at 42°C, followed by 70°C for 5 min to inactivate the enzyme.

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: Paragraph title: RNA Isolation and Semiquantitative and Quantitative RT-PCR Analysis ... RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy).

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: Paragraph title: MSC isolation, culture, expansion, cryopreservation, differentiation and characterization ... Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA).

    Size-exclusion Chromatography:

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy). .. Reactions were performed in an ABI PRISM 7700 Sequence Detection System (Applied Biosystems) using the following parameters: 40 cycles of a two-step PCR program at 95°C for 15 sec and 60°C for 60 sec, after an initial denaturation/activation step at 95°C for 10 min. For semiquantitative RT-PCR determinations, aliquots were harvested from the amplification reactions after 20, 25, 30, and 35 cycles and separated on agarose gel.

    Labeling:

    Article Title: Molecular Mechanisms of Classical Ehlers-Danlos Syndrome
    Article Snippet: Complementary DNA was synthesized from the RNA using the Superscript kit (Gibco BRL). .. Collagenous proteins were labeled and studied as previously described ( ).

    Mouse Assay:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: RNA Isolation and Real-Time PCR Analysis Total RNA from the livers of irradiated and sham-irradiated mice was isolated after homogenization in Trizol® (Invitrogen) according to manufacturers’ protocol. .. For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ].

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: RNA isolation and real-time PCR analysis Total RNA from the livers of irradiated and sham-irradiated mice was isolated after homogenization in Trizol (Invitrogen) as described previously . .. For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen.

    Article Title: Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair
    Article Snippet: Reverse transcription was performed with 1 µg of total RNA using the Superscript Kit (Invitrogen) and random nonamer primers. .. Four mice per genotype and/or treatment group were used in each experiment unless specified otherwise, relative mRNA concentrations were determined using the threshold cycle method and normalized to Rpl8.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer
    Article Snippet: Paragraph title: RNA (RT-PCR) studies ... The mRNA samples were extracted from mouse livers and colorectal specimen, as well as the human colorectal samples, and were DNAase-treated and reverse-transcribed using the SuperScript kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s recommendations using random hexamer primers.

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy). .. Osteocalcin, Acethyl-colinesterase E, transforming growth factor-β (TGF-β1), platelet-derived growth factor-α (PDGF-α), and vascular endothelial growth factor (VEGF) cDNA were quantified by semiquantitative RT-PCR, as described.

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: Reverse transcription was performed with a Superscript kit (Invitrogen). .. BMP2 , BMP4 , noggin , and GAPDH mRNAs were detected by RT–PCR.

    Immunodetection:

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: Reverse transcription was performed with a Superscript kit (Invitrogen). .. For BMP immunodetection, 1 ml of spent-keratinocyte or control media were precipitated with two volumes of acetone, while keratinocytes were pelleted directly by centrifugation.

    Purification:

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: RNAs were purified by Trizol-extraction of keratinocytes (Invitrogen) and spin-column chromatography (Qiagen). .. Reverse transcription was performed with a Superscript kit (Invitrogen).

    SDS Page:

    Article Title: Links between signal transduction, transcription and adhesion in epithelial bud development
    Article Snippet: Reverse transcription was performed with a Superscript kit (Invitrogen). .. Samples were subjected to 10% SDS–PAGE and immunoblotting with anti-BMPs (R & D Systems).

    Plasmid Preparation:

    Article Title: Cadherins mediate sequential roles through a hierarchy of mechanisms in the developing mammillary body
    Article Snippet: A total of 2 μg of DNA per well were transfected (1.8 μg of shRNA plasmid plus 0.2 μg of “target and control” plasmid). .. Forty eight hours after transfection RNA was extracted, treated with DNAse I and reverse transcribed with the Superscript kit (Invitrogen) (2 μg RNA per reaction).

    Real-time Polymerase Chain Reaction:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: .. For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ]. .. Briefly, cDNA was generated by reverse transcription of 1 µg of total RNA using 100 nM of dNTPs, 50 µM of primer oligo dT15, 200 U of moloney murine leukaemia virus reverse transcriptase (M-MLV RT), 16 U of protector RNase inhibitor in RT buffer and 2.5 µL of 0.1 M DTT; real time PCR was performed using a StepOnePlus™ sequence detection system from Applied Biosystems (Darmstadt, Germany) with primer detecting TNF-α-sequence (fwd 5'-CAAACCACCAAGTGGAGGAG-3', rev 3'-GTGGGTGAGGAGCACGTAGT-5'), GAPDH (fwd 5'-AGAACATCATCCCTGCATCC-3', rev 3'-CACATTGGGGGTAGGAACAC-5') and β-actin (fwd 5'-ATTGTTACCAACTGGGACGACATG-3' rev 3'-CGAAGTCTAGAGCAACATAGCACA-5) as housekeeping genes.

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: .. For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen. .. Briefly, cDNA was generated by reverse transcription of 1 μg of total RNA using 100 nM of dNTPs, 50 pM of primer oligo dT15, 200 U of moloney murine leukaemia virus reverse transcriptase (M-MLV RT), 16 U of protector RNase inhibitor in RT-buffer and 2.5 μl of 0.1 M DTT; real-time PCR was performed with a StepOnePlus– sequence detection system (Applied Biosystems, Darmstadt, Germany) as described previously with the primers shown in .

    Article Title: Upregulation of cathepsin D in the caudate nucleus of primates with experimental parkinsonism
    Article Snippet: Paragraph title: RNA isolation and Quantitative Real Time PCR ... In a 50 μ L reaction, reverse transcription (RT) was carried out using the Superscript kit (Invitrogen) for 1 hr at 42°C, followed by 70°C for 5 min to inactivate the RT reagents.

    Article Title: The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection
    Article Snippet: RNA was reverse transcribed using the Superscript kit (Invitrogen, Carlsbad, CA) random hexamer protocol as per the manufacturer’s instructions. .. Real-time PCR analysis was performed on a SmartCycler® (Cepheid, Sunnydale, CA) at 50 °C for 2 min, 95 °C for 10 min, 40 cycles of 95 °C for 15 s and 60 °C for 1 min.

    Article Title: Skeletal mineralization deficits and impaired biogenesis and function of chondrocyte-derived matrix vesicles in Phospho1−/− and Phospho1/Pit1 double knockout mice
    Article Snippet: For a 20 µl reaction, reverse transcription was carried out using the superscript kit (Life technologies, San Diego, CA) for 1 h at 42 °C followed by 70 °C for 5 min to inactivate the RT reagents. .. Specific RNA transcript (mRNA) for Pit1 was quantified by real-time PCR using dual-labeled hydrolysis probes (FAM-TAMRA).

    Article Title: Protective Role for the Disulfide Isomerase PDIA3 in Methamphetamine Neurotoxicity
    Article Snippet: Paragraph title: Quantitative real-time PCR ... In brief, reverse transcription was carried out using the Superscript kit (Life Technologies) at 2 µg RNA per 50 µl reaction, for 1 h at 42°C, followed by 70°C for 5 min to inactivate the enzyme.

    Article Title: Cadherins mediate sequential roles through a hierarchy of mechanisms in the developing mammillary body
    Article Snippet: Paragraph title: Quantitative PCR Control of RNAi in Culture ... Forty eight hours after transfection RNA was extracted, treated with DNAse I and reverse transcribed with the Superscript kit (Invitrogen) (2 μg RNA per reaction).

    Article Title: Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis
    Article Snippet: RT on WT, buc -/- and (−RT) control (from a WT RNA sample) sample was performed using random hexamer primers and the Superscript kit (Invitrogen), followed by RNAaseH reaction according to the kit instructions. .. QPCR was performed using SYBR Green Jumpstart Taq Ready Mix (Sigma-Aldrich), in Step One Plus PCR machine (Applied Biosystems).

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: Phenotype of the BM-MSCs was determined by Real Time quantitative polymerase chain reaction (RT-qPCR). .. Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA).

    shRNA:

    Article Title: Cadherins mediate sequential roles through a hierarchy of mechanisms in the developing mammillary body
    Article Snippet: A total of 2 μg of DNA per well were transfected (1.8 μg of shRNA plasmid plus 0.2 μg of “target and control” plasmid). .. Forty eight hours after transfection RNA was extracted, treated with DNAse I and reverse transcribed with the Superscript kit (Invitrogen) (2 μg RNA per reaction).

    Agarose Gel Electrophoresis:

    Article Title: Removal of the Spleen in Mice Alters the Cytokine Expression Profile of the Marrow Micro-environment and Increases Bone Formation
    Article Snippet: RNA (1 μg) was reverse transcribed with 2.5 μmol/L random hexamers using the superscript kit (InVitrogen, Milan, Italy). .. Reactions were performed in an ABI PRISM 7700 Sequence Detection System (Applied Biosystems) using the following parameters: 40 cycles of a two-step PCR program at 95°C for 15 sec and 60°C for 60 sec, after an initial denaturation/activation step at 95°C for 10 min. For semiquantitative RT-PCR determinations, aliquots were harvested from the amplification reactions after 20, 25, 30, and 35 cycles and separated on agarose gel.

    Homogenization:

    Article Title: The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
    Article Snippet: RNA Isolation and Real-Time PCR Analysis Total RNA from the livers of irradiated and sham-irradiated mice was isolated after homogenization in Trizol® (Invitrogen) according to manufacturers’ protocol. .. For real-time PCR, reverse transcription of the extracted RNA samples was performed using a Superscript kit from Invitrogen as described previously [ ].

    Article Title: Role of PECAM-1 in radiation-induced liver inflammation
    Article Snippet: RNA isolation and real-time PCR analysis Total RNA from the livers of irradiated and sham-irradiated mice was isolated after homogenization in Trizol (Invitrogen) as described previously . .. For real-time PCR, reverse transcription of the extracted RNA samples was performed with a Superscript kit from Invitrogen.

    Spectrophotometry:

    Article Title: Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair
    Article Snippet: RNA preparation, cDNA synthesis, realtime PCR and microarray analysis RNA was isolated from sciatic nerves using the RNeasy Kit (Qiagen) according to manufacturer’s instructions and the concentration and quality (ratio of absorption at 260/280 nm) evaluated using the NanoDrop spectrophotometer. .. Reverse transcription was performed with 1 µg of total RNA using the Superscript Kit (Invitrogen) and random nonamer primers.

    Concentration Assay:

    Article Title: Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair
    Article Snippet: RNA preparation, cDNA synthesis, realtime PCR and microarray analysis RNA was isolated from sciatic nerves using the RNeasy Kit (Qiagen) according to manufacturer’s instructions and the concentration and quality (ratio of absorption at 260/280 nm) evaluated using the NanoDrop spectrophotometer. .. Reverse transcription was performed with 1 µg of total RNA using the Superscript Kit (Invitrogen) and random nonamer primers.

    Staining:

    Article Title: Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints
    Article Snippet: Differentiation ability was evaluated for each lineage by specific staining, using Alizarin Red S (Sigma-Aldrich), Oil Red O and Alcian Blue for osteogenic, adipogenic and chondrogenic differentiation, respectively. .. Afterwards, reverse transcription of 1 μg of total RNA to complementary DNA was performed using the Superscript kit (Invitrogen, Carlsbad, CA, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher superscript iv first strand synthesis system
    Superscript Iv First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iv first strand synthesis system/product/Thermo Fisher
    Average 90 stars, based on 116 article reviews
    Price from $9.99 to $1999.99
    superscript iv first strand synthesis system - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher qrt pcr kit
    Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr kit/product/Thermo Fisher
    Average 90 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    qrt pcr kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher taqman microrna reverse transcription kit
    Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman microrna reverse transcription kit/product/Thermo Fisher
    Average 90 stars, based on 458 article reviews
    Price from $9.99 to $1999.99
    taqman microrna reverse transcription kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results