superscript kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher superscript kit
    Superscript Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript kit/product/Thermo Fisher
    Average 90 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    superscript kit - by Bioz Stars, 2020-07
    90/100 stars

    Images

    Related Articles

    Filtration:

    Article Title: Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms
    Article Snippet: .. After removal of hydrolysis ions by G50 Sephadex filtration (USA Scientific catalog # 1415-1602), the fragmented mRNA was random primed with hexamers and reverse-transcribed using the Super Script II cDNA synthesis kit (Invitrogen catalog # 11917010). .. After second strand synthesis, the cDNA went through end-repair and ligation reactions according to the Illumina ChIP-Seq genomic DNA preparation kit protocol (Illumina catalog # IP102-1001), using the paired end adapters and amplification primers (Illumina Catalog # PE102-1004).

    Synthesized:

    Article Title: MasABK Proteins Interact with Proteins of the Type IV Pilin System to Affect Social Motility of Myxococcus xanthus
    Article Snippet: .. Double-stranded cDNAs were synthesized using Invitrogen's SuperScript cDNA Synthesis Kit with the standard protocol using random hexanucleotide primers (Invitrogen, Carlsbad, CA). .. RNA was harvested from WT, MxH2604 and MxH2627 grown in liquid culture to a density of 5×108 cells/ml as described elsewhere . cDNAs were fluorescently labeled with Cy3 using the standard NimbleGen Gene Expression Analysis protocol.

    Article Title: Differential Gene Expression in CD8+ Cells from HIV-1 Infected Subjects Showing Suppression of HIV Replication
    Article Snippet: .. Complementary DNA (cDNA) was synthesized with the SuperScript™ Double Stranded cDNA Synthesis Kit (Invitrogen Corporation, Carlsbad, CA, USA) using an oligo (dT) primer containing the T7 RNA polymerase site. .. The cDNA was then subjected to an in vitro transcription (IVT) reaction using the T7 RNA polymerase to generate a biotin-labeled complementary RNA (cRNA) (Enzo BioArray™ High Yield™ RNA Transcript Labeling kit, Enzo Life Sciences Inc, Farmingdale, NY, USA) according to the manufacturer instructions.

    Article Title: The E2A-HLF Oncoprotein Activates Groucho-Related Genes and Suppresses Runx1
    Article Snippet: .. Double-stranded cDNAs were synthesized with the Superscript cDNA synthesis kit (GIBCO-BRL) according to the manufacturer's protocol. .. Double-stranded cDNA (2 μg) isolated from both cells grown in the presence of zinc and cells deprived of zinc was digested with Dpn II, ligated with adapter, and amplified by PCR ( , ).

    Random Primed:

    Article Title: Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms
    Article Snippet: .. After removal of hydrolysis ions by G50 Sephadex filtration (USA Scientific catalog # 1415-1602), the fragmented mRNA was random primed with hexamers and reverse-transcribed using the Super Script II cDNA synthesis kit (Invitrogen catalog # 11917010). .. After second strand synthesis, the cDNA went through end-repair and ligation reactions according to the Illumina ChIP-Seq genomic DNA preparation kit protocol (Illumina catalog # IP102-1001), using the paired end adapters and amplification primers (Illumina Catalog # PE102-1004).

    Spectrophotometry:

    Article Title: Conserved Subgroups and Developmental Regulation in the Monocot rop Gene Family 1 Gene Family 1 [w]
    Article Snippet: .. RNA concentrations were determined by spectrophotometry, and cDNA was generated from 5 μg of total RNA using oligo-d(T) primers and the SuperScript cDNA Synthesis kit (Invitrogen). .. To determine relative expression levels of the rop genes, we used MTRP ( ) with each of the three primer sets.

    Generated:

    Article Title: Conserved Subgroups and Developmental Regulation in the Monocot rop Gene Family 1 Gene Family 1 [w]
    Article Snippet: .. RNA concentrations were determined by spectrophotometry, and cDNA was generated from 5 μg of total RNA using oligo-d(T) primers and the SuperScript cDNA Synthesis kit (Invitrogen). .. To determine relative expression levels of the rop genes, we used MTRP ( ) with each of the three primer sets.

    cDNA-AFLP Assay:

    Article Title: Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of ‘Président Roulin’ against Venturia inaequalis
    Article Snippet: .. Double stranded cDNA was finally obtained starting from 500 ng mRNA following the instructions of the Superscript Double Stranded cDNA Synthesis kit (Invitrogen Inc.). cDNA-AFLP analysis was performed with the AFLP Core Reagent kit (Invitrogen Inc.) as recommended by the manufacturer. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher superscript iii first strand synthesis system
    Effect of ETR on the expression of iNOS and proinflammatory cytokines in LPS-stimulated RAW264.7 cells. (A) Expression levels of iNOS mRNA (top) and protein (bottom) were determined by <t>RT-PCR</t> and western blotting. GAPDH and β-actin served as internal controls for RT-PCR and western blotting, respectively. (B) Expression levels of TNF-α, IL-1β and IL-6 mRNA were determined by RT-PCR. (C) Concentrations of IL-1β, TNF-α and IL-6 cytokines in cell culture supernatants were determined by ELISA. Data are presented as the mean ± standard deviation of <t>three</t> independent replicates. *P
    Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii first strand synthesis system/product/Thermo Fisher
    Average 99 stars, based on 4953 article reviews
    Price from $9.99 to $1999.99
    superscript iii first strand synthesis system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of ETR on the expression of iNOS and proinflammatory cytokines in LPS-stimulated RAW264.7 cells. (A) Expression levels of iNOS mRNA (top) and protein (bottom) were determined by RT-PCR and western blotting. GAPDH and β-actin served as internal controls for RT-PCR and western blotting, respectively. (B) Expression levels of TNF-α, IL-1β and IL-6 mRNA were determined by RT-PCR. (C) Concentrations of IL-1β, TNF-α and IL-6 cytokines in cell culture supernatants were determined by ELISA. Data are presented as the mean ± standard deviation of three independent replicates. *P

    Journal: Molecular Medicine Reports

    Article Title: Regulatory effects and molecular mechanism of Trigonostemon reidioides on lipopolysaccharide-induced inflammatory responses in RAW264.7 cells

    doi: 10.3892/mmr.2017.7297

    Figure Lengend Snippet: Effect of ETR on the expression of iNOS and proinflammatory cytokines in LPS-stimulated RAW264.7 cells. (A) Expression levels of iNOS mRNA (top) and protein (bottom) were determined by RT-PCR and western blotting. GAPDH and β-actin served as internal controls for RT-PCR and western blotting, respectively. (B) Expression levels of TNF-α, IL-1β and IL-6 mRNA were determined by RT-PCR. (C) Concentrations of IL-1β, TNF-α and IL-6 cytokines in cell culture supernatants were determined by ELISA. Data are presented as the mean ± standard deviation of three independent replicates. *P

    Article Snippet: RT-PCR was performed using the SuperScript® III First-Strand Synthesis System (Thermo Fisher Scientific, Inc.) and Taq DNA polymerase (Invitrogen; Thermo Fisher Scientific, Inc.).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation

    β maj globin gene expression affects β min globin gene activation by a looped enhancer. ( A ) Interaction frequency determined by chromatin conformation capture (3C) between locations across β-globin locus using HS2 enhancer as the anchor (gray bar) observed for induced ΔCore, ΔCore/GATA and ΔCore/GATA/KLF1 MEL cell lines and uninduced and induced control MEL cells. Yellow triangles along bottom of X axis indicate BglII restriction sites. ( B ) Primary transcripts of β maj and β min globin genes were examined by RT-qPCR after deletion of Core, Core/GATA or ΔCore/GATA/KLF1 elements in the β maj globin gene promoter in induced MEL cells. ( C ) RNA PolII occupancy at β maj and β min globin genes in control, ΔCore, ΔCore/GATA and ΔCore/GATA/KLF1 induced MEL cell lines determined by ChIP-qPCR. ( D ) KLF1 occupancy at β maj and β min globin genes in control, ΔCore, ΔCore/GATA and ΔCore/GATA/KLF1 induced MEL cell lines determined by ChIP-qPCR. Error bars indicate SEM of three biological replicates. (*) P

    Journal: Nucleic Acids Research

    Article Title: LDB1-mediated enhancer looping can be established independent of mediator and cohesin

    doi: 10.1093/nar/gkx433

    Figure Lengend Snippet: β maj globin gene expression affects β min globin gene activation by a looped enhancer. ( A ) Interaction frequency determined by chromatin conformation capture (3C) between locations across β-globin locus using HS2 enhancer as the anchor (gray bar) observed for induced ΔCore, ΔCore/GATA and ΔCore/GATA/KLF1 MEL cell lines and uninduced and induced control MEL cells. Yellow triangles along bottom of X axis indicate BglII restriction sites. ( B ) Primary transcripts of β maj and β min globin genes were examined by RT-qPCR after deletion of Core, Core/GATA or ΔCore/GATA/KLF1 elements in the β maj globin gene promoter in induced MEL cells. ( C ) RNA PolII occupancy at β maj and β min globin genes in control, ΔCore, ΔCore/GATA and ΔCore/GATA/KLF1 induced MEL cell lines determined by ChIP-qPCR. ( D ) KLF1 occupancy at β maj and β min globin genes in control, ΔCore, ΔCore/GATA and ΔCore/GATA/KLF1 induced MEL cell lines determined by ChIP-qPCR. Error bars indicate SEM of three biological replicates. (*) P

    Article Snippet: RNA was reverse transcribed using SuperScript® III First-Strand Synthesis System following manufacturer's instructions (Thermo Fisher Scientific) with random hexamers (Life Technologies).

    Techniques: Expressing, Activation Assay, Quantitative RT-PCR, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

    Expression of fruit bat tetherin is IFN-inducible and required for robust IFN-mediated inhibition of VSV infection. (A) A549 cells and the indicated fruit bat cell lines were incubated with increasing amounts of pan-IFN-α, inoculated with a single-cycle VSV vector bearing VSV-G and encoding luciferase (VSV*ΔG-FLuc), and the luciferase activity in cell lysates was quantified. The luciferase activity measured for cells treated with medium without pan-IFN-α was set as 100%. The results of a representative experiment performed with triplicate samples are shown and were confirmed in a separate experiment. Error bars indicate the SEM. The 50% inhibitory concentration (IC 50 ) was calculated for each cell line and is indicated. (B) Human (A549) and fruit bat (EpoNi/22.1) cells were pan-IFN-α or mock treated, and cellular RNA was extracted, reverse transcribed into cDNA, and analyzed for transcript levels of β-actin, Mx1 (myxovirus resistance protein), and tetherin by qRT-PCR. Shown are combined data (given as the fold change in expression upon IFN treatment, normalized to β-actin) of three independent experiments. Error bars indicate the SEM. (C) Human (A549) and fruit bat (EpoNi/22.1) cells were transfected with the indicated siRNAs (ns, nonsense = control) or left untransfected and then either treated with pan-IFN-α or mock treated, followed by inoculation with replication-competent VSV encoding eGFP. Finally, infected cells (as determined by the expression of eGFP) were detected via fluorescence microscopy. Similar results were obtained in five separate experiments. (D) Viral titers in the cellular supernatants of the untransfected, IFN-α- or mock-treated cells described in panel C were quantified. The relative (x-fold) differences between viral titers in the supernatants of pan-IFN-α- and mock-treated cells were calculated. The average of six independent experiments performed with triplicate samples is shown. Error bars indicate the SEM. (E) Viral titers in the cellular supernatants of the siRNA-transfected and IFN-treated cells described in panel C were quantified. The relative (x-fold) differences between viral titers of pan-IFN-α-treated cells transfected with control or tetherin-specific siRNA were calculated. The average of six independent experiments performed with triplicate samples is shown. Error bars indicate the SEM. (F) Relative tetherin transcript levels in IFN-treated fruit bat cells (EpoNi/22.1), which were previously transfected with either control (ns) or fruit bat tetherin-specific (batTetherin) siRNA, were compared by qRT-PCR (normalized against β-actin transcript levels). Shown are the combined results of three independent experiments performed with triplicate samples, in which tetherin transcript levels of IFN-treated cells that received control siRNA were set as 100%. Error bars indicate the SEM. (G) Human (A549) and fruit bat (EpoNi/22.1) cells were transfected with control or tetherin-specific siRNA prior to treatment with pan-IFN-α. At 48 h posttransfection, the cells were inoculated with single-cycle VSV vector pseudotyped with VSV-G (VSV*ΔG-FLuc). After incubation for 1 h, the cells were washed and further incubated for 8 h before virus-encoded luciferase activity was quantified in the cell lysates. Shown are normalized data from three independent experiments performed with quadruplicate samples in which transduction of cells without prior IFN treatment was set as 100%. Error bars indicate the SEM. The statistical significance of the data presented in panels D and E was analyzed by using a Mann-Whitney U test, while the data presented in panels F and G were analyzed by using a paired, two-tailed Student t test (ns, P > 0.05; **, P ≤ 0.01).

    Journal: Journal of Virology

    Article Title: Tetherin Inhibits Nipah Virus but Not Ebola Virus Replication in Fruit Bat Cells

    doi: 10.1128/JVI.01821-18

    Figure Lengend Snippet: Expression of fruit bat tetherin is IFN-inducible and required for robust IFN-mediated inhibition of VSV infection. (A) A549 cells and the indicated fruit bat cell lines were incubated with increasing amounts of pan-IFN-α, inoculated with a single-cycle VSV vector bearing VSV-G and encoding luciferase (VSV*ΔG-FLuc), and the luciferase activity in cell lysates was quantified. The luciferase activity measured for cells treated with medium without pan-IFN-α was set as 100%. The results of a representative experiment performed with triplicate samples are shown and were confirmed in a separate experiment. Error bars indicate the SEM. The 50% inhibitory concentration (IC 50 ) was calculated for each cell line and is indicated. (B) Human (A549) and fruit bat (EpoNi/22.1) cells were pan-IFN-α or mock treated, and cellular RNA was extracted, reverse transcribed into cDNA, and analyzed for transcript levels of β-actin, Mx1 (myxovirus resistance protein), and tetherin by qRT-PCR. Shown are combined data (given as the fold change in expression upon IFN treatment, normalized to β-actin) of three independent experiments. Error bars indicate the SEM. (C) Human (A549) and fruit bat (EpoNi/22.1) cells were transfected with the indicated siRNAs (ns, nonsense = control) or left untransfected and then either treated with pan-IFN-α or mock treated, followed by inoculation with replication-competent VSV encoding eGFP. Finally, infected cells (as determined by the expression of eGFP) were detected via fluorescence microscopy. Similar results were obtained in five separate experiments. (D) Viral titers in the cellular supernatants of the untransfected, IFN-α- or mock-treated cells described in panel C were quantified. The relative (x-fold) differences between viral titers in the supernatants of pan-IFN-α- and mock-treated cells were calculated. The average of six independent experiments performed with triplicate samples is shown. Error bars indicate the SEM. (E) Viral titers in the cellular supernatants of the siRNA-transfected and IFN-treated cells described in panel C were quantified. The relative (x-fold) differences between viral titers of pan-IFN-α-treated cells transfected with control or tetherin-specific siRNA were calculated. The average of six independent experiments performed with triplicate samples is shown. Error bars indicate the SEM. (F) Relative tetherin transcript levels in IFN-treated fruit bat cells (EpoNi/22.1), which were previously transfected with either control (ns) or fruit bat tetherin-specific (batTetherin) siRNA, were compared by qRT-PCR (normalized against β-actin transcript levels). Shown are the combined results of three independent experiments performed with triplicate samples, in which tetherin transcript levels of IFN-treated cells that received control siRNA were set as 100%. Error bars indicate the SEM. (G) Human (A549) and fruit bat (EpoNi/22.1) cells were transfected with control or tetherin-specific siRNA prior to treatment with pan-IFN-α. At 48 h posttransfection, the cells were inoculated with single-cycle VSV vector pseudotyped with VSV-G (VSV*ΔG-FLuc). After incubation for 1 h, the cells were washed and further incubated for 8 h before virus-encoded luciferase activity was quantified in the cell lysates. Shown are normalized data from three independent experiments performed with quadruplicate samples in which transduction of cells without prior IFN treatment was set as 100%. Error bars indicate the SEM. The statistical significance of the data presented in panels D and E was analyzed by using a Mann-Whitney U test, while the data presented in panels F and G were analyzed by using a paired, two-tailed Student t test (ns, P > 0.05; **, P ≤ 0.01).

    Article Snippet: Next, 1 µg of RNA was used as a template for cDNA synthesis using the SuperScript III first-strand synthesis system (Thermo Fisher Scientific) according to the manufacturer’s protocol (for random hexamers).

    Techniques: Expressing, Inhibition, Infection, Incubation, Plasmid Preparation, Luciferase, Activity Assay, Concentration Assay, Quantitative RT-PCR, Transfection, Fluorescence, Microscopy, Transduction, MANN-WHITNEY, Two Tailed Test

    Inhibition of mTOR-related genes and mTORC1 downstream effectors by RAD001. Notes: qPCR detection of mTOR-related genes, including ( A ) mTOR complexes, ( B ) mTORC1-positive regulation, and ( C ) mTORC2-positive regulation in T24 cells treated with 1 or 5 µM RAD001. Cells were seeded 24 hours before RAD001 treatment. Total RNA samples were extracted at 24 hours posttreatment, reverse transcribed, and subjected to the detection of genes involved in mTOR signaling. The values are shown as the mean ± SD of three independent experiments; * P

    Journal: Drug Design, Development and Therapy

    Article Title: Autophagy inhibition enhances RAD001-induced cytotoxicity in human bladder cancer cells

    doi: 10.2147/DDDT.S95900

    Figure Lengend Snippet: Inhibition of mTOR-related genes and mTORC1 downstream effectors by RAD001. Notes: qPCR detection of mTOR-related genes, including ( A ) mTOR complexes, ( B ) mTORC1-positive regulation, and ( C ) mTORC2-positive regulation in T24 cells treated with 1 or 5 µM RAD001. Cells were seeded 24 hours before RAD001 treatment. Total RNA samples were extracted at 24 hours posttreatment, reverse transcribed, and subjected to the detection of genes involved in mTOR signaling. The values are shown as the mean ± SD of three independent experiments; * P

    Article Snippet: Two micrograms of total RNA was subjected to reverse transcription using SuperScript III First-Strand Synthesis System (Thermo Fisher Scientific). qPCR was performed as previously described.

    Techniques: Inhibition, Real-time Polymerase Chain Reaction