superscript iii system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript iii system
    Superscript Iii System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii system/product/Thermo Fisher
    Average 99 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    superscript iii system - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Murine Hematopoietic Stem Cells and Multipotent Progenitors Express Truncated Intracellular Form of c-kit Receptor
    Article Snippet: .. The RNA from multiple batches of sorted cells (purity 98–99.5%) was isolated with RNeasy Micro Kit (Qiagen), and the RNA equivalents of 2 × 103 to 5 × 104 cells were reverse transcribed and amplified using SuperSCRIPT III System (Invitrogen). .. PCR reactions were performed in an Eppendorf Mastercyler for 35 cycles (95°C for 30 sec, 57–62°C for 45 sec, 72°C for 30 sec).

    Article Title: Molecular dissection of the pea shoot apical meristem *
    Article Snippet: RT- and real time-PCR For reverse transcription (RT)-PCR studies, 1 μg of DNA-free RNA extract was converted into first-strand cDNA using the SuperScript III system for first-strand cDNA synthesis (Invitrogen) and oligo(dT)12–18 . .. 2 μl of cDNA samples were amplified in a 25 μl PCR mixture.

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: Briefly, 500 ng of DNA isolated from the prostate were subjected to PCR amplification designed to detect polyomaviruses and HPV [ , , ]. .. For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ].

    Synthesized:

    Article Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
    Article Snippet: .. Complementary DNA was synthesized from 2 μg of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. Real-time PCR analysis was performed with 0.5 μl of the final cDNA synthesis mix using commercially produced rat-specific Taq-Man-based gene expression assays (Applied Biosystems, Foster City, CA).

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: .. Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. Real-time PCR analysis was performed with 0.5 ul of the final cDNA synthesis mix using commercially produced rat specific Taq-Man based gene expression assays (Applied Biosystems, Foster City, CA).

    Article Title: Salvianolic Acid B Inhibits Growth of Head and Neck Squamous Cell Carcinoma in vitro and in vivo via Cyclooxygenase-2 and Apoptotic Pathways
    Article Snippet: .. First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-1 and COX-2 were measured by taking advantage of real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-1 primer (6-FAM labeled probe, hs00168776_m1), or COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: Combination Effects of Salvianolic Acid B with Low Dose Celecoxib on Inhibition of Head and Neck Squamous Cell Carcinoma Growth in vitro and in vivo
    Article Snippet: .. First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-2 were measured by real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Quantitative RT-PCR:

    Article Title: Salvianolic Acid B Inhibits Growth of Head and Neck Squamous Cell Carcinoma in vitro and in vivo via Cyclooxygenase-2 and Apoptotic Pathways
    Article Snippet: Paragraph title: Real-time RT-PCR ... First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA).

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Purification was estimated through Bioanalyzer picoRNA chips (Agilent Technologies) and by RT-qPCR using specific primers for 18S rRNA and GAPDH (see for primer description). .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Article Title: Mesenchymal progenitor cells derived from traumatized human muscle
    Article Snippet: .. The RNA samples were reverse transcribed with the SuperScript III System for qRT-PCR (Invitrogen) using Oligo-dT and random hexamers. .. The expression levels of osteogenic, adipogenic, chondrogenic genes were assayed by conventional PCR using Platinum Taq (Invitrogen) following the manufacturer’s protocol (See for gene primers, annealing temperatures, PCR cycles and product sizes).

    Article Title: Combination Effects of Salvianolic Acid B with Low Dose Celecoxib on Inhibition of Head and Neck Squamous Cell Carcinoma Growth in vitro and in vivo
    Article Snippet: First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-2 were measured by real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: An epiblast stem cell-derived multipotent progenitor population for axial extension
    Article Snippet: Paragraph title: Quantitative RT-PCR (qRT-PCR) ... First-strand cDNA synthesis was performed with the Superscript III system (Invitrogen).

    Real-time Polymerase Chain Reaction:

    Article Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
    Article Snippet: Paragraph title: Real-time PCR analysis. ... Complementary DNA was synthesized from 2 μg of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA).

    Article Title: Molecular dissection of the pea shoot apical meristem *
    Article Snippet: .. RT- and real time-PCR For reverse transcription (RT)-PCR studies, 1 μg of DNA-free RNA extract was converted into first-strand cDNA using the SuperScript III system for first-strand cDNA synthesis (Invitrogen) and oligo(dT)12–18 . .. 2 μl of cDNA samples were amplified in a 25 μl PCR mixture.

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: Paragraph title: Real-time PCR Analysis ... Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA).

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche). .. To synthesize isotope-labeled D3-ac4C, cytidine (200 mg, 0.82 mmol) and D6 -acetic anhydride (78 μL, 0.82 mmol, Sigma-Aldrich) were dissolved in methanol (4 mL) and heated to reflux with stirring.

    Article Title: Combination Effects of Salvianolic Acid B with Low Dose Celecoxib on Inhibition of Head and Neck Squamous Cell Carcinoma Growth in vitro and in vivo
    Article Snippet: Paragraph title: Quantitative Real-time PCR ... First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA).

    Article Title: PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated With Host Persistence and Virulence
    Article Snippet: Paragraph title: RNA Isolation and Reverse Transcription qPCR ... One microgram of RNA was then used for reverse transcription (RT) with random primers, using the SuperScript III system (Thermo Fisher Scientifc, USA), as described elsewhere ( ).

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: Paragraph title: Real-time quantitative PCR (qPCR) assays ... A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures.

    Expressing:

    Article Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
    Article Snippet: Complementary DNA was synthesized from 2 μg of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. Real-time PCR analysis was performed with 0.5 μl of the final cDNA synthesis mix using commercially produced rat-specific Taq-Man-based gene expression assays (Applied Biosystems, Foster City, CA).

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. Real-time PCR analysis was performed with 0.5 ul of the final cDNA synthesis mix using commercially produced rat specific Taq-Man based gene expression assays (Applied Biosystems, Foster City, CA).

    Article Title: Salvianolic Acid B Inhibits Growth of Head and Neck Squamous Cell Carcinoma in vitro and in vivo via Cyclooxygenase-2 and Apoptotic Pathways
    Article Snippet: First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-1 and COX-2 were measured by taking advantage of real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-1 primer (6-FAM labeled probe, hs00168776_m1), or COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: Neuro-mesodermal progenitors (NMPs): a comparative study between pluripotent stem cells and embryo-derived populations
    Article Snippet: First-strand cDNA synthesis was performed using the Superscript III system (Invitrogen) and the quantification of double-stranded DNA was obtained using specific primers (see using QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q). .. Expression values were normalized against the housekeeping gene Ppia .

    Article Title: Mesenchymal progenitor cells derived from traumatized human muscle
    Article Snippet: The RNA samples were reverse transcribed with the SuperScript III System for qRT-PCR (Invitrogen) using Oligo-dT and random hexamers. .. The expression levels of osteogenic, adipogenic, chondrogenic genes were assayed by conventional PCR using Platinum Taq (Invitrogen) following the manufacturer’s protocol (See for gene primers, annealing temperatures, PCR cycles and product sizes).

    Article Title: Combination Effects of Salvianolic Acid B with Low Dose Celecoxib on Inhibition of Head and Neck Squamous Cell Carcinoma Growth in vitro and in vivo
    Article Snippet: First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-2 were measured by real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: Expression analysis was performed at three developmental stages of fruit development (pre-bloom, I; bloom, J; and fruit set with berries showing 5-10 mm equatorial diameter, K) according to Baggiolini [ ]). .. A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures.

    Article Title: An epiblast stem cell-derived multipotent progenitor population for axial extension
    Article Snippet: First-strand cDNA synthesis was performed with the Superscript III system (Invitrogen). .. Expression values were normalized against the housekeeping gene Ppia .

    Modification:

    Article Title: PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated With Host Persistence and Virulence
    Article Snippet: RNA was isolated using a modified protocol from the RNeasy Mini kit (Qiagen) described elsewhere ( ). .. One microgram of RNA was then used for reverse transcription (RT) with random primers, using the SuperScript III system (Thermo Fisher Scientifc, USA), as described elsewhere ( ).

    Derivative Assay:

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: Three biological samples where independently analyzed for each genotype × stage combination. qPCR was performed with the LightCycler® (Roche Diagnostics) real-time PCR system using SYBR Green® as the fluorescent dye to measure DNA amplicons derived from mRNA. .. A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures.

    Cell Culture:

    Article Title: Neuro-mesodermal progenitors (NMPs): a comparative study between pluripotent stem cells and embryo-derived populations
    Article Snippet: This population of cells was compared with the EM2-RFP4d (Epi-NMP RFP cells plated in a flask and cultured for 4 days in N2B27 supplemented with 20 ng/ml FGF2 and 3 μM Chiron to make Epi-meso2). .. First-strand cDNA synthesis was performed using the Superscript III system (Invitrogen) and the quantification of double-stranded DNA was obtained using specific primers (see using QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q).

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Total RNA was purified from cultured cells using Trizol (ThermoFisher Scientific) followed by treatment with Turbo™ DNase I (ThermoFisher Scientific). .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Molecular dissection of the pea shoot apical meristem *
    Article Snippet: .. RT- and real time-PCR For reverse transcription (RT)-PCR studies, 1 μg of DNA-free RNA extract was converted into first-strand cDNA using the SuperScript III system for first-strand cDNA synthesis (Invitrogen) and oligo(dT)12–18 . .. 2 μl of cDNA samples were amplified in a 25 μl PCR mixture.

    Article Title: The transmembrane inner ear (tmie) gene contributes to vestibular and lateral line development and function in the zebrafish (Danio rerio)
    Article Snippet: Paragraph title: tmie gene identification and RT-PCR analysis ... RNA was reverse-transcribed with the Superscript III system (Invitrogen).

    Article Title: Mesenchymal progenitor cells derived from traumatized human muscle
    Article Snippet: Paragraph title: 2.7 Reverse Transcription-Polymerase Chain Reaction ... The RNA samples were reverse transcribed with the SuperScript III System for qRT-PCR (Invitrogen) using Oligo-dT and random hexamers.

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures. .. For each gene, a calibration curve was constructed by measuring the fluorescence of four serial dilutions (101 -10-2 pg ul-1 ) of an RT-PCR product obtained with the same oligos and cDNA from PN40024 as the template to estimate copy numbers in total cDNA.

    Sequencing:

    Article Title: The transmembrane inner ear (tmie) gene contributes to vestibular and lateral line development and function in the zebrafish (Danio rerio)
    Article Snippet: The mouse Tmie protein sequence was used to search the zebrafish genome using the TBLASTN algorithm ( ). .. RNA was reverse-transcribed with the Superscript III system (Invitrogen).

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: Paragraph title: Viral sequence detection ... For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ].

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: Real-time quantitative PCR (qPCR) assays The transcript abundance of VvAGL11 was evaluated in the four genotypes of the RS × S progeny described above for sequence characterization: both heterozygous seedless parents of the progeny (Ruby Seedless and Sultanina), and an unrelated seeded common table grape genotype, Red Globe. .. A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures.

    Fluorescence:

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures. .. For each gene, a calibration curve was constructed by measuring the fluorescence of four serial dilutions (101 -10-2 pg ul-1 ) of an RT-PCR product obtained with the same oligos and cDNA from PN40024 as the template to estimate copy numbers in total cDNA.

    Isolation:

    Article Title: Murine Hematopoietic Stem Cells and Multipotent Progenitors Express Truncated Intracellular Form of c-kit Receptor
    Article Snippet: .. The RNA from multiple batches of sorted cells (purity 98–99.5%) was isolated with RNeasy Micro Kit (Qiagen), and the RNA equivalents of 2 × 103 to 5 × 104 cells were reverse transcribed and amplified using SuperSCRIPT III System (Invitrogen). .. PCR reactions were performed in an Eppendorf Mastercyler for 35 cycles (95°C for 30 sec, 57–62°C for 45 sec, 72°C for 30 sec).

    Article Title: Neuro-mesodermal progenitors (NMPs): a comparative study between pluripotent stem cells and embryo-derived populations
    Article Snippet: Total RNA was isolated from the three samples (EM2-RFP4d, EM2-RFP+4d and EM2-RFP-4d) using TRIzol (Invitrogen/Thermo Fisher Scientific). .. First-strand cDNA synthesis was performed using the Superscript III system (Invitrogen) and the quantification of double-stranded DNA was obtained using specific primers (see using QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q).

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Paragraph title: Isolation of total and polyadenylated RNA ... Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Article Title: PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated With Host Persistence and Virulence
    Article Snippet: Paragraph title: RNA Isolation and Reverse Transcription qPCR ... One microgram of RNA was then used for reverse transcription (RT) with random primers, using the SuperScript III system (Thermo Fisher Scientifc, USA), as described elsewhere ( ).

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: Briefly, 500 ng of DNA isolated from the prostate were subjected to PCR amplification designed to detect polyomaviruses and HPV [ , , ]. .. For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ].

    Article Title: An epiblast stem cell-derived multipotent progenitor population for axial extension
    Article Snippet: Total RNA was isolated from cells using Trizol. .. First-strand cDNA synthesis was performed with the Superscript III system (Invitrogen).

    Size-exclusion Chromatography:

    Article Title: Murine Hematopoietic Stem Cells and Multipotent Progenitors Express Truncated Intracellular Form of c-kit Receptor
    Article Snippet: The RNA from multiple batches of sorted cells (purity 98–99.5%) was isolated with RNeasy Micro Kit (Qiagen), and the RNA equivalents of 2 × 103 to 5 × 104 cells were reverse transcribed and amplified using SuperSCRIPT III System (Invitrogen). .. PCR reactions were performed in an Eppendorf Mastercyler for 35 cycles (95°C for 30 sec, 57–62°C for 45 sec, 72°C for 30 sec).

    Labeling:

    Article Title: Salvianolic Acid B Inhibits Growth of Head and Neck Squamous Cell Carcinoma in vitro and in vivo via Cyclooxygenase-2 and Apoptotic Pathways
    Article Snippet: First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-1 and COX-2 were measured by taking advantage of real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-1 primer (6-FAM labeled probe, hs00168776_m1), or COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: Combination Effects of Salvianolic Acid B with Low Dose Celecoxib on Inhibition of Head and Neck Squamous Cell Carcinoma Growth in vitro and in vivo
    Article Snippet: First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-2 were measured by real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Purification:

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Purification was estimated through Bioanalyzer picoRNA chips (Agilent Technologies) and by RT-qPCR using specific primers for 18S rRNA and GAPDH (see for primer description). .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Article Title: PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated With Host Persistence and Virulence
    Article Snippet: Further RNA purification steps were performed as recommended by the manufacturer. .. One microgram of RNA was then used for reverse transcription (RT) with random primers, using the SuperScript III system (Thermo Fisher Scientifc, USA), as described elsewhere ( ).

    Dot Blot:

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Enrichment of polyadenylated RNA [poly(A) RNA] was achieved through two rounds of selection with Oligo-(dT)25 Dynabeads (ThermoFisher Scientific) for LC-MS, dot blot or acRIP-seq, or two rounds of poly(A)Purist MAG (ThermoFisher Scientific) for ImmunoNorthern blot, according to the manufacturer’s instructions. .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Polymerase Chain Reaction:

    Article Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
    Article Snippet: Complementary DNA was synthesized from 2 μg of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. The PCR reactions were performed in an ABI 7500 real-time PCR thermocycler (Applied Biosystems).

    Article Title: Murine Hematopoietic Stem Cells and Multipotent Progenitors Express Truncated Intracellular Form of c-kit Receptor
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and polymerase chain reaction ... The RNA from multiple batches of sorted cells (purity 98–99.5%) was isolated with RNeasy Micro Kit (Qiagen), and the RNA equivalents of 2 × 103 to 5 × 104 cells were reverse transcribed and amplified using SuperSCRIPT III System (Invitrogen).

    Article Title: Molecular dissection of the pea shoot apical meristem *
    Article Snippet: RT- and real time-PCR For reverse transcription (RT)-PCR studies, 1 μg of DNA-free RNA extract was converted into first-strand cDNA using the SuperScript III system for first-strand cDNA synthesis (Invitrogen) and oligo(dT)12–18 . .. 2 μl of cDNA samples were amplified in a 25 μl PCR mixture.

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. The PCR reactions were performed in an I-Cyler real-time PCR thermocycler (BioRad).

    Article Title: Salvianolic Acid B Inhibits Growth of Head and Neck Squamous Cell Carcinoma in vitro and in vivo via Cyclooxygenase-2 and Apoptotic Pathways
    Article Snippet: .. First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-1 and COX-2 were measured by taking advantage of real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-1 primer (6-FAM labeled probe, hs00168776_m1), or COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: The transmembrane inner ear (tmie) gene contributes to vestibular and lateral line development and function in the zebrafish (Danio rerio)
    Article Snippet: RNA was reverse-transcribed with the Superscript III system (Invitrogen). .. PCR was performed using Advantage Taq polymerase (BD Biosciences-Clontech).

    Article Title: Neuro-mesodermal progenitors (NMPs): a comparative study between pluripotent stem cells and embryo-derived populations
    Article Snippet: .. First-strand cDNA synthesis was performed using the Superscript III system (Invitrogen) and the quantification of double-stranded DNA was obtained using specific primers (see using QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q). ..

    Article Title: Mesenchymal progenitor cells derived from traumatized human muscle
    Article Snippet: The RNA samples were reverse transcribed with the SuperScript III System for qRT-PCR (Invitrogen) using Oligo-dT and random hexamers. .. The expression levels of osteogenic, adipogenic, chondrogenic genes were assayed by conventional PCR using Platinum Taq (Invitrogen) following the manufacturer’s protocol (See for gene primers, annealing temperatures, PCR cycles and product sizes).

    Article Title: Combination Effects of Salvianolic Acid B with Low Dose Celecoxib on Inhibition of Head and Neck Squamous Cell Carcinoma Growth in vitro and in vivo
    Article Snippet: .. First-strand cDNA was synthesized by PCR using a SuperScript III system with oligo(dT) primers (Invitrogen, Carlsbad, CA). .. The expression levels of COX-2 were measured by real-time RT-PCR using TaqMan@ Universal PCR Master Mix (Applied Biosystems, Foster City, CA) with COX-2 primer (6-FAM labeled probe, Hs00153133_m1).

    Article Title: PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated With Host Persistence and Virulence
    Article Snippet: One microgram of RNA was then used for reverse transcription (RT) with random primers, using the SuperScript III system (Thermo Fisher Scientifc, USA), as described elsewhere ( ). .. Quantitative PCR was performed in a StepOne real-time PCR system (Thermo Fisher USA) in reaction samples (10 μl) containing 1 μl of cDNA samples, 1X Power SYBR green PCR master mix (Lifetech), and 10 μM of each primer for 16SrRNA or for pcsB ( ).

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: Briefly, 500 ng of DNA isolated from the prostate were subjected to PCR amplification designed to detect polyomaviruses and HPV [ , , ]. .. For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ].

    Article Title: An epiblast stem cell-derived multipotent progenitor population for axial extension
    Article Snippet: First-strand cDNA synthesis was performed with the Superscript III system (Invitrogen). .. The quantification of double-stranded DNA obtained for primer-specific genes was achieved with QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q).

    Construct:

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures. .. For each gene, a calibration curve was constructed by measuring the fluorescence of four serial dilutions (101 -10-2 pg ul-1 ) of an RT-PCR product obtained with the same oligos and cDNA from PN40024 as the template to estimate copy numbers in total cDNA.

    Nested PCR:

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: Viral sequences were identified in prostate tissue by nested PCR as previously described [ , , ]. .. For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ].

    Activated Clotting Time Assay:

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. The following assays were used: Beta-actin (Act b, catalog # Rn00667869_m1) and Vascular endothelial growth factor (Vegf, catalog # Rn00582935_m1).

    SYBR Green Assay:

    Article Title: Neuro-mesodermal progenitors (NMPs): a comparative study between pluripotent stem cells and embryo-derived populations
    Article Snippet: .. First-strand cDNA synthesis was performed using the Superscript III system (Invitrogen) and the quantification of double-stranded DNA was obtained using specific primers (see using QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q). ..

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche). .. To synthesize isotope-labeled D3-ac4C, cytidine (200 mg, 0.82 mmol) and D6 -acetic anhydride (78 μL, 0.82 mmol, Sigma-Aldrich) were dissolved in methanol (4 mL) and heated to reflux with stirring.

    Article Title: PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated With Host Persistence and Virulence
    Article Snippet: One microgram of RNA was then used for reverse transcription (RT) with random primers, using the SuperScript III system (Thermo Fisher Scientifc, USA), as described elsewhere ( ). .. Quantitative PCR was performed in a StepOne real-time PCR system (Thermo Fisher USA) in reaction samples (10 μl) containing 1 μl of cDNA samples, 1X Power SYBR green PCR master mix (Lifetech), and 10 μM of each primer for 16SrRNA or for pcsB ( ).

    Article Title: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine
    Article Snippet: Three biological samples where independently analyzed for each genotype × stage combination. qPCR was performed with the LightCycler® (Roche Diagnostics) real-time PCR system using SYBR Green® as the fluorescent dye to measure DNA amplicons derived from mRNA. .. A 100-ng aliquot of mRNA was used as the template for reverse transcription reactions to synthesize single-stranded cDNA using the SuperScript III® system and oligo(dT) primers (INVITROGEN) according to standard procedures.

    Article Title: An epiblast stem cell-derived multipotent progenitor population for axial extension
    Article Snippet: First-strand cDNA synthesis was performed with the Superscript III system (Invitrogen). .. The quantification of double-stranded DNA obtained for primer-specific genes was achieved with QuantiFast SYBR Green PCR Master Mix (Qiagen) and the standard cycler program (Qiagen RotorGene Q).

    RNA Extraction:

    Article Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
    Article Snippet: Total RNA was extracted from frozen brain cortex using an RNA extraction kit (RNAgents; Promega, Madison, WI). .. Complementary DNA was synthesized from 2 μg of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA).

    Article Title: Murine Hematopoietic Stem Cells and Multipotent Progenitors Express Truncated Intracellular Form of c-kit Receptor
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and polymerase chain reaction ... The RNA from multiple batches of sorted cells (purity 98–99.5%) was isolated with RNeasy Micro Kit (Qiagen), and the RNA equivalents of 2 × 103 to 5 × 104 cells were reverse transcribed and amplified using SuperSCRIPT III System (Invitrogen).

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: Total RNA was extracted from frozen brain cortex using an RNA extraction kit (RNAgents; Promega, Madison, WI). .. Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA).

    Selection:

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Enrichment of polyadenylated RNA [poly(A) RNA] was achieved through two rounds of selection with Oligo-(dT)25 Dynabeads (ThermoFisher Scientific) for LC-MS, dot blot or acRIP-seq, or two rounds of poly(A)Purist MAG (ThermoFisher Scientific) for ImmunoNorthern blot, according to the manufacturer’s instructions. .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Produced:

    Article Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain
    Article Snippet: Complementary DNA was synthesized from 2 μg of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. Real-time PCR analysis was performed with 0.5 μl of the final cDNA synthesis mix using commercially produced rat-specific Taq-Man-based gene expression assays (Applied Biosystems, Foster City, CA).

    Article Title: Hypoxia Inducible Factor-1 (HIF-1) Independent Microvascular Angiogenesis in the Aged Rat Brain
    Article Snippet: Complimentary DNA was synthesized from 2 ug of total RNA using the Superscript III system with an oligo-dT primer (Invitrogen, Carlsbad, CA). .. Real-time PCR analysis was performed with 0.5 ul of the final cDNA synthesis mix using commercially produced rat specific Taq-Man based gene expression assays (Applied Biosystems, Foster City, CA).

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ]. .. The HCMV detection was performed in two amplification rounds using sets of primers specific for the UL3 region, which produced amplicons of 420 and 188 bp, consecutively.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: Enrichment of polyadenylated RNA [poly(A) RNA] was achieved through two rounds of selection with Oligo-(dT)25 Dynabeads (ThermoFisher Scientific) for LC-MS, dot blot or acRIP-seq, or two rounds of poly(A)Purist MAG (ThermoFisher Scientific) for ImmunoNorthern blot, according to the manufacturer’s instructions. .. Briefly, RNA was reverse transcribed (RT) with random hexamers using the Superscript III system (ThermoFisher Scientific) according to the manufacturer’s suggestions, followed by qPCR using LightCycler 480 SYBR Green I Master Mix (Roche, Basil, Switzerland) in a LightCycler 96 Instrument (Roche).

    Staining:

    Article Title: Identification of viral infections in the prostate and evaluation of their association with cancer
    Article Snippet: The reaction products were electrophoresed through 3% agarose gels and visualized by ethidium bromide staining. .. For XMRV screening, 1 μg of RNA was reverse transcribed using the SSIII system (Invitrogen) and 2 μL of the cDNA reaction were used in the protocol described by Urisman et al. [ ].

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