superscript iii reverse transcriptase (Thermo Fisher)


Name:
Shandon 10000 Autopsy Saw
Description:
Cut safely and reduce operator fatigue with Thermo Scientific Shandon 10000 Autopsy Saw
Catalog Number:
10000
Price:
None
Category:
Instruments and Equipment
Applications:
Anatomical Pathology|Clinical
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Cut safely and reduce operator fatigue with Thermo Scientific Shandon 10000 Autopsy Saw
https://www.bioz.com/result/superscript iii reverse transcriptase/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "TET enzymes augment activation-induced deaminase (AID) expression via 5-hydroxymethylcytosine modifications at the Aicda superenhancer"
Article Title: TET enzymes augment activation-induced deaminase (AID) expression via 5-hydroxymethylcytosine modifications at the Aicda superenhancer
Journal: Science immunology
doi: 10.1126/sciimmunol.aau7523

Figure Legend Snippet: TET proteins facilitate CSR. ( A ) Flowchart of in vivo experiment. f.p., footpad. ( B ) Top: Flow cytometric analysis of CD19 + GL7 + Fas + GC B cells at the draining popliteal lymph nodes from WT and Tet2/3 DKO mice after NP-OVA immunization as in (A). Bottom: Decreased IgG1-switched cells among GC B cells in Tet2/3 DKO (YFP + GC BâÂÂgated) compared with WT mice (GC BâÂÂgated). ( C to F ) Quantification of experiments shown in (B). Data shown are aggregated results from two independent experiments. Means and SEs are shown. WT, n = 11; DKO, n = 12. ( G ) Flowchart of in vitro IgG1 switching. Cells were labeled with Cell trace violet and activated for 4 days with LPS and IL-4. ( H and I ) Flow cytometry plots (H) and quantification (I) of IgG1-switched B cells in Tet2/3 DKO ( n = 4) and WT ( n = 4) mice. Representative of at least three independent experiments. ( J ) Circular ó1 transcript was quantified by qRT-PCR and normalized to Gapdh and then to the level of WT. Representative experiment of two independent experiments with three technical replicates. ( K ) Flowchart of in vitro IgA switching. Cells were activated for 5 days with anti-CD40, rmIL-4, rmIL-5, and rhTGFò. ( L to N ) Flow cytometry plots (L) and quantification (M and N) of IgG1-switched (M) and IgA-switched cells (N). Representative of three independent experiments with three technical replicates. Statistical significance was calculated using unpaired two-tailed t test. * P
Techniques Used: In Vivo, Flow Cytometry, Mouse Assay, In Vitro, Labeling, Cytometry, Quantitative RT-PCR, Two Tailed Test

Figure Legend Snippet: TET2/3 facilitate CSR by regulating expression of the cytidine deaminase AID. ( A ) qRT-PCR analysis of Aicda mRNA expression in WT and Tet2/3 DKO B cells activated 4 days with LPS and IL-4. Aicda expression was normalized to Gapdh and then to the level in WT. Data shown are representative of two independent experiments with three technical replicates. * P
Techniques Used: Expressing, Quantitative RT-PCR
2) Product Images from "A comprehensive evaluation of Hippo pathway silencing in sarcomas"
Article Title: A comprehensive evaluation of Hippo pathway silencing in sarcomas
Journal: Oncotarget
doi: 10.18632/oncotarget.25824

Figure Legend Snippet: ( A ) Summary of cell lines diagram. Expression of the Hippo kinases was lost at the protein level in 0% (LATS1) to 58% (MST1) of the sarcoma cell lines, indicated by (+). Accumulation of the Hippo kinases with treatment with MG132, indicated by (+), was noted only for MST2, indicating that proteosomal degradation is an important mechanism by which MST2 expression is lost. Loss of expression at the RNA level was identified for MST1 (42%) and MST2 (25%) of sarcoma cell lines. Loss of expression at the RNA level for LATS1 and LATS2 was negligible. Deletions of the Hippo kinases were essentially absent from the sarcoma cell lines with the exception of LATS2 , where 1 of the 12 sarcoma cell lines (8%) demonstrated a deletion. Treatment with 10 μM 5-azacytidine resulted in a modest increase in expression in 8–17% of the sarcoma cell lines. Treatment with 0.5 μM TSA resulted in a reversal of expression in a higher percentage of cell lines, predominantly with MST1 and MST2 . Treatment with TSA and 5-azacytidine showed an additive effect with regards to re-expressing the Hippo kinases in some cell lines. ( B ) Expression of the Hippo kinases is regulated by at least three different mechanisms shown in this model, potentially targetable by different therapeutic interventions. Promoter (CpG island) hypermethylation is one mechanism that appears to modestly regulate the expression of predominantly MST1 and MST2 . Histone deacetylation can also promote silencing the expression of the Hippo kinases, again particularly MST1 and MST2 , and to a lesser degree LATS2 . Proteosomal degradation plays a role in in regulating the expression of MST2 and could be targeted as well.
Techniques Used: Expressing
3) Product Images from "1α,25(OH)2D3-glycosides from Solanum glaucophyllum leaves extract induce myoblasts differentiation through p38 MAPK and AKT activation"
Article Title: 1α,25(OH)2D3-glycosides from Solanum glaucophyllum leaves extract induce myoblasts differentiation through p38 MAPK and AKT activation
Journal: Biology Open
doi: 10.1242/bio.033670

Figure Legend Snippet: Genes regulation by SGE or synthetic 1α,25(OH) 2 D 3 during onset of differentiation. C2C12 cells were treated with SGE (10 nM), 1α,25(OH) 2 D 3 (1,25D, 1 nM), or vehicle alone (ctrl) in DM in time course experiments (24–72 h). Total RNA was extracted and reverse transcribed and data analysis was performed for each gene expression by qRT-PCR. Bar graphs show quantitative results from three experiments performed in duplicate and expressed as a ratio between mRNA of each gene (A) myoD1 , (B) myogenin , (C) MHC2b in DM under control conditions (ctrl) or treated (1,25D or SGE) previously normalized to GAPDH mRNA levels and to GM (time 0). The results were then represented in bar graphs. Significant differences between control and treated (SGE or 1,25D) conditions at each time point were analyzed by Student's t -test. ns: not significant. * P
Techniques Used: Expressing, Quantitative RT-PCR
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