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TaKaRa superscript iii reverse transcriptase
Validation by real-time RT-PCR of DNA <t>microarray</t> results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of <t>three</t> independent replicates. *, Significant difference ( P
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1) Product Images from "Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿"

Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿

Journal: Journal of Virology

doi: 10.1128/JVI.02220-09

Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P
Figure Legend Snippet: Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

Techniques Used: Quantitative RT-PCR, Microarray, Expressing

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Amplification:

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Synthesized:

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Quantitative RT-PCR:

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Article Title: Down-Regulation of miR-146a Expression Induces Allergic Conjunctivitis in Mice by Increasing TSLP Level
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Real-time Polymerase Chain Reaction:

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Microarray:

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Quantitation Assay:

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Expressing:

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Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿
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Transformation Assay:

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Infection:

Article Title: Comprehensive Analysis of Soybean Mosaic Virus P3 Protein Interactors and Hypersensitive Response-Like Lesion-Inducing Protein Function
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Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿
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Reverse Transcription Polymerase Chain Reaction:

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Sequencing:

Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿
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Isolation:

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Article Title: Comprehensive Analysis of Soybean Mosaic Virus P3 Protein Interactors and Hypersensitive Response-Like Lesion-Inducing Protein Function
Article Snippet: Soybean cDNA Library and Bait Vector Construction To construct the library, total RNA was isolated from soybean floral after SMV-SC15 strain infection using the RNA TRIzol Reagent (Invitrogen, Carlsbad, CA, USA). mRNA was isolated according to FastTrack® 2.0 Kit’s manufacturer’s procedure. .. The first-strand cDNA was synthesized from 3 μg of mRNA using attB2-Oligo (dT) primer by SuperScript III reverse transcriptase (Takara Bio, Dalian, China).

Article Title: Advanced Glycation Endproducts on Stored Erythrocytes increase Endothelial Reactive Oxygen Species Generation through Interaction with RAGE
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Article Title: Rapid genomic and transcriptomic alterations induced by wide hybridization: Chrysanthemum nankingense x Tanacetum vulgare and C. crassum x Crossostephium chinense (Asteraceae)
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Article Title: Down-Regulation of miR-146a Expression Induces Allergic Conjunctivitis in Mice by Increasing TSLP Level
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Purification:

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Polymerase Chain Reaction:

Article Title: Osteoblast-targeted delivery of miR-33-5p attenuates osteopenia development induced by mechanical unloading in mice
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Construct:

Article Title: Comprehensive Analysis of Soybean Mosaic Virus P3 Protein Interactors and Hypersensitive Response-Like Lesion-Inducing Protein Function
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cDNA Library Assay:

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Plasmid Preparation:

Article Title: Comprehensive Analysis of Soybean Mosaic Virus P3 Protein Interactors and Hypersensitive Response-Like Lesion-Inducing Protein Function
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SYBR Green Assay:

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Article Title: Activation of the Nucleotide Oligomerization Domain Signaling Pathway by the Non-bacterially Derived Xanthone Drug 5?6-Dimethylxanthenone-4-acetic Acid (Vadimezan) *
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Article Title: Advanced Glycation Endproducts on Stored Erythrocytes increase Endothelial Reactive Oxygen Species Generation through Interaction with RAGE
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Article Title: MicroRNA-544 inhibits glioma proliferation, invasion and migration but induces cell apoptosis by targeting PARK7
Article Snippet: The cDNA was synthesized using RT primer and SuperScript III reverse transcriptase following the manufacturer’s instructions (Takara, Japan). .. Quantitative RT-PCR was performed using iQTM 5 Multicolor Real-Time PCR Detection system (BioRad) with Realtime PCR Master Mix (SYBR Green, Toyobo, Osaka, Japan).

RNA Extraction:

Article Title: Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza
Article Snippet: Paragraph title: RNA extraction and quantitative real-time reverse transcription-PCR (qRT-PCR) ... RNA quantity was determined using a NanoDrop 2000C Spectrophotometer (Thermo Scientific, USA). cDNA synthesis was carried out using Superscript III Reverse Transcriptase (TaKaRa, China). qRT-PCRs were performed using the SYBR premix Ex Taq™ kit (TaKaRa, China) and carried out in triplicate for each tissue sample.

Agarose Gel Electrophoresis:

Article Title: Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats
Article Snippet: Quantification and quality assessment of RNA was carried out with Nano Drop spectrophotometer (Thermo Scientific) and running on 1% agarose gel prepared in DEPC-treated TBE buffer. .. RNA was treated with DNase before cDNA synthesis. cDNA was synthesized using 2 µg RNA with superscript-III reverse transcriptase (Takara, USA).

Article Title: Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza
Article Snippet: RNA integrity was analyzed on a 1.2% agarose gel. .. RNA quantity was determined using a NanoDrop 2000C Spectrophotometer (Thermo Scientific, USA). cDNA synthesis was carried out using Superscript III Reverse Transcriptase (TaKaRa, China). qRT-PCRs were performed using the SYBR premix Ex Taq™ kit (TaKaRa, China) and carried out in triplicate for each tissue sample.

Ethanol Precipitation:

Article Title: Rapid genomic and transcriptomic alterations induced by wide hybridization: Chrysanthemum nankingense x Tanacetum vulgare and C. crassum x Crossostephium chinense (Asteraceae)
Article Snippet: The first cDNA strand was synthesized from a 300 ng RNA based on random priming and SuperScript III Reverse Transcriptase (Takara, EC 2.7.7.49). .. The second strand was then synthesized by the addition of 10 U DNA polymerase I (Takara, EC 2.7.7.7) and 5 U RNase H (Takara, EC 3.1.26.4) [ ], and purified by extraction in phenol: chloroform: isoamyl alcohol (25:24:1, v/v) followed by ethanol precipitation.

Article Title: MicroRNA-544 inhibits glioma proliferation, invasion and migration but induces cell apoptosis by targeting PARK7
Article Snippet: The RNA was recovered using phenol/chloroform extraction followed by ethanol precipitation. .. The cDNA was synthesized using RT primer and SuperScript III reverse transcriptase following the manufacturer’s instructions (Takara, Japan).

Random Hexamer Labeling:

Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿
Article Snippet: .. For validation of microarray data, total RNA was reverse transcribed into cDNAs using SuperScript III reverse transcriptase and random hexamer (TaKaRa Bio). .. Primer and probe sets for relative quantification of human genes were selected from the product list of TaqMan gene expression assays (Applied Biosystems).

Spectrophotometry:

Article Title: Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats
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Article Title: Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza
Article Snippet: .. RNA quantity was determined using a NanoDrop 2000C Spectrophotometer (Thermo Scientific, USA). cDNA synthesis was carried out using Superscript III Reverse Transcriptase (TaKaRa, China). qRT-PCRs were performed using the SYBR premix Ex Taq™ kit (TaKaRa, China) and carried out in triplicate for each tissue sample. ..

Article Title: Down-Regulation of miR-146a Expression Induces Allergic Conjunctivitis in Mice by Increasing TSLP Level
Article Snippet: The purity of all RNA samples was quantified by NanoDrop® ND-1000 Spectrophotometer and stored at −80°C. .. The first strand cDNA was synthesized by RT from 1 μg of total RNA using superscript III reverse transcriptase (Takara, Tokyo, Japan).

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Article Title: MicroRNA-544 inhibits glioma proliferation, invasion and migration but induces cell apoptosis by targeting PARK7
Article Snippet: The RNA concentration was estimated by measuring the absorbance at 260 nm using a NanoDrop 2000c (Thermo Scientific). .. The cDNA was synthesized using RT primer and SuperScript III reverse transcriptase following the manufacturer’s instructions (Takara, Japan).

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    TaKaRa superscript iii reverse transcriptase
    Validation by real-time RT-PCR of DNA <t>microarray</t> results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of <t>three</t> independent replicates. *, Significant difference ( P
    Superscript Iii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

    Journal: Journal of Virology

    Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿

    doi: 10.1128/JVI.02220-09

    Figure Lengend Snippet: Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

    Article Snippet: For validation of microarray data, total RNA was reverse transcribed into cDNAs using SuperScript III reverse transcriptase and random hexamer (TaKaRa Bio).

    Techniques: Quantitative RT-PCR, Microarray, Expressing