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Keygen Biotech superscript iii reverse transcriptase
(a) Overall elastin (R2) after fractional carbon dioxide (CO 2 ) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO 2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori's aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μ m. (c) Total <t>RNA</t> was extracted from biopsied skin samples of different groups. Procollagen types I and <t>III</t> and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD ( n = 3). The asterisk (∗) indicates a significant difference ( P
Superscript Iii Reverse Transcriptase, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing"

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing

Journal: BioMed Research International

doi: 10.1155/2013/519126

(a) Overall elastin (R2) after fractional carbon dioxide (CO 2 ) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO 2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori's aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μ m. (c) Total RNA was extracted from biopsied skin samples of different groups. Procollagen types I and III and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD ( n = 3). The asterisk (∗) indicates a significant difference ( P
Figure Legend Snippet: (a) Overall elastin (R2) after fractional carbon dioxide (CO 2 ) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO 2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori's aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μ m. (c) Total RNA was extracted from biopsied skin samples of different groups. Procollagen types I and III and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD ( n = 3). The asterisk (∗) indicates a significant difference ( P

Techniques Used: Staining, Quantitative RT-PCR

2) Product Images from "The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study"

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study

Journal: PLoS ONE

doi: 10.1371/journal.pone.0099703

Baicalin protects against UVB irradiation-induced type III collagen fiber loss. (A) Skin specimens of dorsal trunk were harvested for immuno-staining using primary antibodies of procollagen type III collagen. Images are representative of results from 5 tissue samples. Original magnification ×40. (B) Total RNA was extracted from biopsied skin samples of different groups. Type III procollagen mRNA was determined by real-time RT-PCR analysis. Results are shown as means±SD (n = 5). The symbol (#) indicates a significant difference (p
Figure Legend Snippet: Baicalin protects against UVB irradiation-induced type III collagen fiber loss. (A) Skin specimens of dorsal trunk were harvested for immuno-staining using primary antibodies of procollagen type III collagen. Images are representative of results from 5 tissue samples. Original magnification ×40. (B) Total RNA was extracted from biopsied skin samples of different groups. Type III procollagen mRNA was determined by real-time RT-PCR analysis. Results are shown as means±SD (n = 5). The symbol (#) indicates a significant difference (p

Techniques Used: Irradiation, Immunostaining, Quantitative RT-PCR

Related Articles

Reverse Transcription Polymerase Chain Reaction:

Article Title: The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts
Article Snippet: Paragraph title: 2.5. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) ... In brief, we applied TRIzol to extract the total RNAs from HDFs and then synthesized the cDNAs from the separated RNA using SuperScript III Reverse Transcriptase (KeyGen, China).

Microarray:

Article Title: The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts
Article Snippet: Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) In order to confirm the results obtained from microarray, we applied qRT-PCR to remeasure the abundance of differentially expressed lncRNAs and mRNAs selected from microarray analysis. .. In brief, we applied TRIzol to extract the total RNAs from HDFs and then synthesized the cDNAs from the separated RNA using SuperScript III Reverse Transcriptase (KeyGen, China).

Isolation:

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
Article Snippet: .. Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study
Article Snippet: .. Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Synthesized:

Article Title: The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts
Article Snippet: .. In brief, we applied TRIzol to extract the total RNAs from HDFs and then synthesized the cDNAs from the separated RNA using SuperScript III Reverse Transcriptase (KeyGen, China). .. QRT-PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
Article Snippet: .. Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study
Article Snippet: .. Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Quantitative RT-PCR:

Article Title: The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts
Article Snippet: Paragraph title: 2.5. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) ... In brief, we applied TRIzol to extract the total RNAs from HDFs and then synthesized the cDNAs from the separated RNA using SuperScript III Reverse Transcriptase (KeyGen, China).

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study
Article Snippet: Paragraph title: Real-time RT-PCR detection ... Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China).

Polymerase Chain Reaction:

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
Article Snippet: Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study
Article Snippet: Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Expressing:

Article Title: The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts
Article Snippet: In brief, we applied TRIzol to extract the total RNAs from HDFs and then synthesized the cDNAs from the separated RNA using SuperScript III Reverse Transcriptase (KeyGen, China). .. The reactions were performed at 9°C for 10 min, then at 40 cycles at 95°C for 15 s, and followed by at 60°C for 30 s. The 2 (−ΔΔCt) methods were used to evaluate relative quantification of lncRNAs and mRNAs expression.

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
Article Snippet: Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. For each gene, fold-change was calculated as the difference in gene expression between two groups.

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study
Article Snippet: Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. For each gene, fold-change was calculated as the difference in gene expression between two groups.

Sequencing:

Article Title: The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts
Article Snippet: In brief, we applied TRIzol to extract the total RNAs from HDFs and then synthesized the cDNAs from the separated RNA using SuperScript III Reverse Transcriptase (KeyGen, China). .. QRT-PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
Article Snippet: Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study
Article Snippet: Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China). .. PCR was performed on ABI Prism 7700 Sequence Detector (Applied Biosystems).

Staining:

Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
Article Snippet: Serial sections (4 mm) were mounted onto silane-coated slides and stained by H & E, Masson-Trichrome, and Gomori's aldehyde fuchsin staining, respectively. .. Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China).

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    Keygen Biotech superscript iii reverse transcriptase
    (a) Overall elastin (R2) after fractional carbon dioxide (CO 2 ) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO 2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori's aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μ m. (c) Total <t>RNA</t> was extracted from biopsied skin samples of different groups. Procollagen types I and <t>III</t> and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD ( n = 3). The asterisk (∗) indicates a significant difference ( P
    Superscript Iii Reverse Transcriptase, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Keygen Biotech
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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    (a) Overall elastin (R2) after fractional carbon dioxide (CO 2 ) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO 2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori's aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μ m. (c) Total RNA was extracted from biopsied skin samples of different groups. Procollagen types I and III and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD ( n = 3). The asterisk (∗) indicates a significant difference ( P

    Journal: BioMed Research International

    Article Title: The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing

    doi: 10.1155/2013/519126

    Figure Lengend Snippet: (a) Overall elastin (R2) after fractional carbon dioxide (CO 2 ) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO 2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori's aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μ m. (c) Total RNA was extracted from biopsied skin samples of different groups. Procollagen types I and III and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD ( n = 3). The asterisk (∗) indicates a significant difference ( P

    Article Snippet: Total RNA was extracted from biopsy skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China).

    Techniques: Staining, Quantitative RT-PCR

    Baicalin protects against UVB irradiation-induced type III collagen fiber loss. (A) Skin specimens of dorsal trunk were harvested for immuno-staining using primary antibodies of procollagen type III collagen. Images are representative of results from 5 tissue samples. Original magnification ×40. (B) Total RNA was extracted from biopsied skin samples of different groups. Type III procollagen mRNA was determined by real-time RT-PCR analysis. Results are shown as means±SD (n = 5). The symbol (#) indicates a significant difference (p

    Journal: PLoS ONE

    Article Title: The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study

    doi: 10.1371/journal.pone.0099703

    Figure Lengend Snippet: Baicalin protects against UVB irradiation-induced type III collagen fiber loss. (A) Skin specimens of dorsal trunk were harvested for immuno-staining using primary antibodies of procollagen type III collagen. Images are representative of results from 5 tissue samples. Original magnification ×40. (B) Total RNA was extracted from biopsied skin samples of different groups. Type III procollagen mRNA was determined by real-time RT-PCR analysis. Results are shown as means±SD (n = 5). The symbol (#) indicates a significant difference (p

    Article Snippet: Total RNA was extracted from skin samples by using TRIzol (Invitrogen, USA). cDNA was synthesized from the isolated RNA using SuperScript III Reverse Transcriptase (Keygen, China).

    Techniques: Irradiation, Immunostaining, Quantitative RT-PCR