superscript iii reverse transcriptase thermo scientific  (Thermo Fisher)


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    Thermo Fisher superscript iii reverse transcriptase thermo scientific
    Superscript Iii Reverse Transcriptase Thermo Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase thermo scientific/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase thermo scientific - by Bioz Stars, 2020-07
    92/100 stars

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    Article Title: Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification
    Article Snippet: .. For the amplification following the Isothermal Mastermix protocol, the total volume of 25 μl per reaction tube included 15 μl Isothermal Master Mix, 5 μl template, 5 μl Primer Mix and 0.1 μl SuperScript® III Reverse Transcriptase (Thermo Scientific). ..

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    Thermo Fisher superscript iii reverse transcriptase
    Accumulation ( A ) and silencing activity ( B ) of cholesterol-modified siRNAs and TsiRNAs in a carrier-free mode in vitro. ( A ) Accumulation of monomer (Ch-siRNA) and trimeric (Ch-TsiRNA-2) cholesterol-modified siRNAs in KB-8-5 cells 4 h after addition (1 µM), measured by stem-loop <t>RT-PCR.</t> B) Silencing activity of Ch-siRNA, Ch-TsiRNA-1 and Ch-TsiRNA-2 <t>three</t> days after addition (5 µM) to KB-8-5-MDR1-GFP or KB-3-1-MDR1-GFP cells, fluorescence intensity value of untreated cells was used as a 100%.
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 7903 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-07
    99/100 stars
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    Accumulation ( A ) and silencing activity ( B ) of cholesterol-modified siRNAs and TsiRNAs in a carrier-free mode in vitro. ( A ) Accumulation of monomer (Ch-siRNA) and trimeric (Ch-TsiRNA-2) cholesterol-modified siRNAs in KB-8-5 cells 4 h after addition (1 µM), measured by stem-loop RT-PCR. B) Silencing activity of Ch-siRNA, Ch-TsiRNA-1 and Ch-TsiRNA-2 three days after addition (5 µM) to KB-8-5-MDR1-GFP or KB-3-1-MDR1-GFP cells, fluorescence intensity value of untreated cells was used as a 100%.

    Journal: Molecules

    Article Title: Trimeric Small Interfering RNAs and Their Cholesterol-Containing Conjugates Exhibit Improved Accumulation in Tumors, but Dramatically Reduced Silencing Activity

    doi: 10.3390/molecules25081877

    Figure Lengend Snippet: Accumulation ( A ) and silencing activity ( B ) of cholesterol-modified siRNAs and TsiRNAs in a carrier-free mode in vitro. ( A ) Accumulation of monomer (Ch-siRNA) and trimeric (Ch-TsiRNA-2) cholesterol-modified siRNAs in KB-8-5 cells 4 h after addition (1 µM), measured by stem-loop RT-PCR. B) Silencing activity of Ch-siRNA, Ch-TsiRNA-1 and Ch-TsiRNA-2 three days after addition (5 µM) to KB-8-5-MDR1-GFP or KB-3-1-MDR1-GFP cells, fluorescence intensity value of untreated cells was used as a 100%.

    Article Snippet: Synthesis of cDNA and stem-loop PCR was carried out using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA); and qPCR mix was assembled using BioMaster qPCR SYBR Blue (Biolabmix, Novosibirsk, Russia).

    Techniques: Activity Assay, Modification, In Vitro, Reverse Transcription Polymerase Chain Reaction, Fluorescence

    XPO5 is required for miRNA biogenesis. a Bioinformatic pipeline of quantitative small RNA-seq and data analysis. b Small RNAs between 20 and 23 nt showed strong depletion in XPO5 cKO skin samples. Small RNA reads from 18 to 27 nt were charted from small RNA cDNA libraries. c The depletion of miRNAs in XPO5 cKO skin is weaker than that in Dicer1 and Dgcr8 cKO skin. For each boxplot, the middle line is the median, the vertical line spans the data range, and the hinges are the first and third quartiles. A two-sided Mann–Whitney test was used for statistical tests. d Depletion of mature miRNA reads in XPO5 cKO skin is evident for highly expressed miRNAs (red coloured dots). e Depletion of mir-17-5p , mir-18 , mir-19a , and mir-20a except mir-92a in XPO5 cKO skin is measured by qPCR. f Depletion of mir-15a , mir-15b , and mir-16 in XPO5 cKO skin is measured by qPCR. g Depletion of mir-17-5p is confirmed by northern blotting. h Unchanged expression of mir-320 and mir-484 in XPO5 cKO skin is measured by qPCR. Data shown are mean s.d. from three independent experiments. ** P

    Journal: Nature Communications

    Article Title: XPO5 promotes primary miRNA processing independently of RanGTP

    doi: 10.1038/s41467-020-15598-x

    Figure Lengend Snippet: XPO5 is required for miRNA biogenesis. a Bioinformatic pipeline of quantitative small RNA-seq and data analysis. b Small RNAs between 20 and 23 nt showed strong depletion in XPO5 cKO skin samples. Small RNA reads from 18 to 27 nt were charted from small RNA cDNA libraries. c The depletion of miRNAs in XPO5 cKO skin is weaker than that in Dicer1 and Dgcr8 cKO skin. For each boxplot, the middle line is the median, the vertical line spans the data range, and the hinges are the first and third quartiles. A two-sided Mann–Whitney test was used for statistical tests. d Depletion of mature miRNA reads in XPO5 cKO skin is evident for highly expressed miRNAs (red coloured dots). e Depletion of mir-17-5p , mir-18 , mir-19a , and mir-20a except mir-92a in XPO5 cKO skin is measured by qPCR. f Depletion of mir-15a , mir-15b , and mir-16 in XPO5 cKO skin is measured by qPCR. g Depletion of mir-17-5p is confirmed by northern blotting. h Unchanged expression of mir-320 and mir-484 in XPO5 cKO skin is measured by qPCR. Data shown are mean s.d. from three independent experiments. ** P

    Article Snippet: For mRNA analysis, 1 μg total RNA was used to synthesize cDNA by Superscript III Reverse Transcriptase (Thermo Fisher Scientific).

    Techniques: RNA Sequencing Assay, MANN-WHITNEY, Real-time Polymerase Chain Reaction, Northern Blot, Expressing

    Molecular and biological properties of tomato torrado virus. a Schematic representation of the ToTV genome. The genomic material of ToTV comprises two polyadenylated RNA strands, RNA1 and RNA2, composed of 7829 nucleotides and 5404 nucleotides, respectively. RNA1 encodes for protease cofactor (PrcCo), helicase (Hel), viral protease (Pro) and RNA-dependent RNA polymerase (RdRP). RNA2 encodes for ORF1 (necessary for systemic infection) and ORF2 encoding movement protein (3A) followed by three capsid protein (CP) subunits (Vp35, Vp26 and Vp23). Dashed lines indicate borders of each CP subunit. b Symptoms induced by ToTV in Solanum lycopersicum cv. Beta Lux

    Journal: Virology Journal

    Article Title: Contribution of Tomato torrado virus Vp26 coat protein subunit to systemic necrosis induction and virus infectivity in Solanum lycopersicum

    doi: 10.1186/s12985-019-1117-9

    Figure Lengend Snippet: Molecular and biological properties of tomato torrado virus. a Schematic representation of the ToTV genome. The genomic material of ToTV comprises two polyadenylated RNA strands, RNA1 and RNA2, composed of 7829 nucleotides and 5404 nucleotides, respectively. RNA1 encodes for protease cofactor (PrcCo), helicase (Hel), viral protease (Pro) and RNA-dependent RNA polymerase (RdRP). RNA2 encodes for ORF1 (necessary for systemic infection) and ORF2 encoding movement protein (3A) followed by three capsid protein (CP) subunits (Vp35, Vp26 and Vp23). Dashed lines indicate borders of each CP subunit. b Symptoms induced by ToTV in Solanum lycopersicum cv. Beta Lux

    Article Snippet: For cloning purposes, cDNA was synthesized using 2 μg of total RNA, 1 μl of 50 μM oligo(dT)18 and 200 U SuperScript III reverse transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.

    Techniques: Infection

    CKII Phosphorylation of Spt6 Regulates Chromatin Integrity during Transcription (A) Schematic of the SRG1 and SER3 loci showing their expression patterns in WT and mutant strains, as indicated by red (WT) and green (mutant) arrows. (B) Representative RNA-seq tracks showing an increase in the expression level of the SER3 gene in WT, spt6 S8 → A8 , spt6 S8 → E8 , and spt6–1004 allele. (C) Quantitative real-time PCR detection of SRG1 and SER3 transcripts in the WT and spt6–1004 mutant strain. (D) Quantitative real-time PCR detection of SRG1 and SER3 transcripts in the WT and spt6 mutant ( spt6 S8 → A8 and spt6 S8 → E8 ) strains. (E) ChIP analysis of histone H3 levels across SRG1 and SER3 was performed with WT and spt6 mutant ( spt6 S8 → A8 and spt6 S8 → E8 ) strains. Amplicons are indicated below the schematic diagram of the genes. Quantitative real-time PCR and ChIP data are represented as means ± SDs of three independent biological experiments. Asterisks indicate significance values (**p

    Journal: Cell reports

    Article Title: Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction

    doi: 10.1016/j.celrep.2018.11.089

    Figure Lengend Snippet: CKII Phosphorylation of Spt6 Regulates Chromatin Integrity during Transcription (A) Schematic of the SRG1 and SER3 loci showing their expression patterns in WT and mutant strains, as indicated by red (WT) and green (mutant) arrows. (B) Representative RNA-seq tracks showing an increase in the expression level of the SER3 gene in WT, spt6 S8 → A8 , spt6 S8 → E8 , and spt6–1004 allele. (C) Quantitative real-time PCR detection of SRG1 and SER3 transcripts in the WT and spt6–1004 mutant strain. (D) Quantitative real-time PCR detection of SRG1 and SER3 transcripts in the WT and spt6 mutant ( spt6 S8 → A8 and spt6 S8 → E8 ) strains. (E) ChIP analysis of histone H3 levels across SRG1 and SER3 was performed with WT and spt6 mutant ( spt6 S8 → A8 and spt6 S8 → E8 ) strains. Amplicons are indicated below the schematic diagram of the genes. Quantitative real-time PCR and ChIP data are represented as means ± SDs of three independent biological experiments. Asterisks indicate significance values (**p

    Article Snippet: The isolated RNA was treated with 10 U of RNase-free DNase (Promega) for 30 minutes, followed by RNA cleanup (QIAGEN RNeasy Mini Kit, 74106). cDNA was synthesized from one mg of total RNA using random hexamer primers and Superscript Reverse Transcriptase III (Thermo-Fisher Scientific, 108–80044).

    Techniques: Expressing, Mutagenesis, RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation