superscript iii reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript iii reverse transcriptase kit
    A posterior program of gene expression is down-regulated after β-catenin-1 inhibition in acoels. (A) Experimental scheme for <t>RNA</t> sequencing. Hofstenia miamia were injected once a day for <t>three</t> consecutive days with control or β-catenin-1 dsRNA. Tails were collected in trizol at 3, 7, and 14 days after the first injection for RNA sequencing. Red box indicates tissue collected. (B) A cluster of 66 genes is down-regulated early after β-catenin-1 RNAi. Heatmap displaying gene expression counts as z-scores for indicated timepoints post first RNAi injection. *indicates annotation by best BLAST hit. Gene list provided in S5 Table . (C) Left: Validation of β-catenin-1 RNAi-dependent down-regulation of gene expression. FISH for indicated transcripts at day 9 post-RNAi initiation in homeostasis. Images are presented in grayscale with color inverted. Right: Genes down-regulated after β-catenin-1 RNAi are expressed in the posterior. FISH for indicated transcripts in two week old hatchlings. Transcript ID provided in parentheses. Images are presented in grayscale with color inverted. Scale bars, 200μm.
    Superscript Iii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A small set of conserved genes, including sp5 and Hox, are activated by Wnt signaling in the posterior of planarians and acoels"

    Article Title: A small set of conserved genes, including sp5 and Hox, are activated by Wnt signaling in the posterior of planarians and acoels

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1008401

    A posterior program of gene expression is down-regulated after β-catenin-1 inhibition in acoels. (A) Experimental scheme for RNA sequencing. Hofstenia miamia were injected once a day for three consecutive days with control or β-catenin-1 dsRNA. Tails were collected in trizol at 3, 7, and 14 days after the first injection for RNA sequencing. Red box indicates tissue collected. (B) A cluster of 66 genes is down-regulated early after β-catenin-1 RNAi. Heatmap displaying gene expression counts as z-scores for indicated timepoints post first RNAi injection. *indicates annotation by best BLAST hit. Gene list provided in S5 Table . (C) Left: Validation of β-catenin-1 RNAi-dependent down-regulation of gene expression. FISH for indicated transcripts at day 9 post-RNAi initiation in homeostasis. Images are presented in grayscale with color inverted. Right: Genes down-regulated after β-catenin-1 RNAi are expressed in the posterior. FISH for indicated transcripts in two week old hatchlings. Transcript ID provided in parentheses. Images are presented in grayscale with color inverted. Scale bars, 200μm.
    Figure Legend Snippet: A posterior program of gene expression is down-regulated after β-catenin-1 inhibition in acoels. (A) Experimental scheme for RNA sequencing. Hofstenia miamia were injected once a day for three consecutive days with control or β-catenin-1 dsRNA. Tails were collected in trizol at 3, 7, and 14 days after the first injection for RNA sequencing. Red box indicates tissue collected. (B) A cluster of 66 genes is down-regulated early after β-catenin-1 RNAi. Heatmap displaying gene expression counts as z-scores for indicated timepoints post first RNAi injection. *indicates annotation by best BLAST hit. Gene list provided in S5 Table . (C) Left: Validation of β-catenin-1 RNAi-dependent down-regulation of gene expression. FISH for indicated transcripts at day 9 post-RNAi initiation in homeostasis. Images are presented in grayscale with color inverted. Right: Genes down-regulated after β-catenin-1 RNAi are expressed in the posterior. FISH for indicated transcripts in two week old hatchlings. Transcript ID provided in parentheses. Images are presented in grayscale with color inverted. Scale bars, 200μm.

    Techniques Used: Expressing, Inhibition, RNA Sequencing Assay, Injection, Fluorescence In Situ Hybridization

    An anterior program of gene expression is up-regulated after β-catenin-1 inhibition in acoels. (A) Experimental scheme for RNA sequencing. Hofstenia miamia were injected once a day for three consecutive days with control or β-catenin-1 dsRNA. Tails were collected in trizol at 3, 7, and 14 days after the first injection for RNA sequencing. Red box indicates tissue collected. (B) 92 genes are up-regulated early after β-catenin-1 RNAi. Heatmap displaying gene expression counts as a z-score for indicated time-points post-RNAi initiation. *indicates annotation by best BLAST hit. Gene list provided in S5 Table . (C) Top: Validation of β-catenin-1 RNAi dependent up-regulation of gene expression. FISH for indicated transcripts at day 9 post-RNAi initiation in homeostasis. Images are presented in grayscale with color inverted. Bottom: Genes up-regulated after β-catenin-1 RNAi are expressed in the anterior. FISH for indicated transcripts in two week old hatchlings. Transcript ID provided in parentheses. Images are presented in grayscale with color inverted. All scale bars, 200μm.
    Figure Legend Snippet: An anterior program of gene expression is up-regulated after β-catenin-1 inhibition in acoels. (A) Experimental scheme for RNA sequencing. Hofstenia miamia were injected once a day for three consecutive days with control or β-catenin-1 dsRNA. Tails were collected in trizol at 3, 7, and 14 days after the first injection for RNA sequencing. Red box indicates tissue collected. (B) 92 genes are up-regulated early after β-catenin-1 RNAi. Heatmap displaying gene expression counts as a z-score for indicated time-points post-RNAi initiation. *indicates annotation by best BLAST hit. Gene list provided in S5 Table . (C) Top: Validation of β-catenin-1 RNAi dependent up-regulation of gene expression. FISH for indicated transcripts at day 9 post-RNAi initiation in homeostasis. Images are presented in grayscale with color inverted. Bottom: Genes up-regulated after β-catenin-1 RNAi are expressed in the anterior. FISH for indicated transcripts in two week old hatchlings. Transcript ID provided in parentheses. Images are presented in grayscale with color inverted. All scale bars, 200μm.

    Techniques Used: Expressing, Inhibition, RNA Sequencing Assay, Injection, Fluorescence In Situ Hybridization

    2) Product Images from "Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering"

    Article Title: Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-019-1255-1

    Deletion of upp gene from Rhodobacter sphaeroides genome. a Number of colonies obtained on RÄ agar plates plated with 10 −3 dilutions of R. sphaeroides conjugation mixtures with the homologous recombination (HR) control vectors pBBR_Cas9_Δupp500HR_NT and pBBR_Cas9_Δupp1000HR_NT, and HR editing vectors pBBR_Cas9_Δupp500HR_sp2 and pBBR_Cas9_Δupp1000HR_sp2. The error bars represent standard deviations from three replicate experiments. b Restreaks of randomly selected colonies from the above mentioned conjugations on RÄ agar plates supplemented with 5-FU. Only Δ upp mutants can grow on 5-FU plates. c Genome specific colony PCR amplification of the upp locus in cells conjugated with the pBBR_Cas9_Δupp500HR_NT, pBBR_Cas9_Δupp1000HR_NT, pBBR_Cas9_Δupp500HR_sp2 and pBBR_Cas9_Δupp1000HR_sp2 vectors. Amplification yields a 2992 bp product for the wild type upp gene and a 2374 bp product for the deleted upp gene. d Sequence verification of the desired upp deletion by Sanger sequencing
    Figure Legend Snippet: Deletion of upp gene from Rhodobacter sphaeroides genome. a Number of colonies obtained on RÄ agar plates plated with 10 −3 dilutions of R. sphaeroides conjugation mixtures with the homologous recombination (HR) control vectors pBBR_Cas9_Δupp500HR_NT and pBBR_Cas9_Δupp1000HR_NT, and HR editing vectors pBBR_Cas9_Δupp500HR_sp2 and pBBR_Cas9_Δupp1000HR_sp2. The error bars represent standard deviations from three replicate experiments. b Restreaks of randomly selected colonies from the above mentioned conjugations on RÄ agar plates supplemented with 5-FU. Only Δ upp mutants can grow on 5-FU plates. c Genome specific colony PCR amplification of the upp locus in cells conjugated with the pBBR_Cas9_Δupp500HR_NT, pBBR_Cas9_Δupp1000HR_NT, pBBR_Cas9_Δupp500HR_sp2 and pBBR_Cas9_Δupp1000HR_sp2 vectors. Amplification yields a 2992 bp product for the wild type upp gene and a 2374 bp product for the deleted upp gene. d Sequence verification of the desired upp deletion by Sanger sequencing

    Techniques Used: Conjugation Assay, Homologous Recombination, Polymerase Chain Reaction, Amplification, Sequencing

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    DNA Synthesis:

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    Synthesized:

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    Quantitative RT-PCR:

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    SYBR Green Assay:

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    Incubation:

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    Activity Assay:

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    Transfection:

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    Sequencing:

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    Cell Culture:

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    Generated:

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    Isolation:

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    Reverse Transcription Polymerase Chain Reaction:

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    Binding Assay:

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    RNA HS Assay:

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    Aqueous Normal-phase Chromatography:

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    Size-exclusion Chromatography:

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    Article Snippet: Complementary DNA (cDNA) was synthesized from 1 µg total RNA using SuperScript III reverse transcriptase kit (Invitrogen-Thermo Fisher Scientific) using oligo dT-primed reactions. .. Reactions were performed in a 10 µl volume of 25 ng/µl cDNA, 250 nM of each primer and 5 µl of QuantiFast SYBR Green PCR Master Mix (Qiagen, CA) on the Biosystems StepOnePlus™ Real-Time PCR System. qPCR condition was at 95 °C for 10 min, 95 °C for 10 sec. and 60 °C for 30 sec for 40 cycles.

    Purification:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: SuperScript III Reverse Transcriptase kit (Life Technologies) was used to synthesize the cDNA. .. The reaction contained 500 ng purified RNA from each sample in a final reaction volume of 20 µl, performed according to manufacturer’s instructions.

    Article Title: A small set of conserved genes, including sp5 and Hox, are activated by Wnt signaling in the posterior of planarians and acoels
    Article Snippet: Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit (Life Technologies). .. 1μg RNA input was used to prepare cDNA with the SuperScript III Reverse Transcriptase kit (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-β pathway
    Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... Total RNA was transcribed into complementary DNA (cDNA) by RT using the SuperScript III Reverse Transcriptase kit (Thermo Fisher Scientific, Inc.).

    Article Title: RETINOL SATURASE MODULATES LIPID METABOLISM AND THE PRODUCTION OF REACTIVE OXYGEN SPECIES
    Article Snippet: Complementary DNA (cDNA) was synthesized from 1 µg total RNA using SuperScript III reverse transcriptase kit (Invitrogen-Thermo Fisher Scientific) using oligo dT-primed reactions. .. Reactions were performed in a 10 µl volume of 25 ng/µl cDNA, 250 nM of each primer and 5 µl of QuantiFast SYBR Green PCR Master Mix (Qiagen, CA) on the Biosystems StepOnePlus™ Real-Time PCR System. qPCR condition was at 95 °C for 10 min, 95 °C for 10 sec. and 60 °C for 30 sec for 40 cycles.

    Article Title: High N-Acetyltransferase 1 Expression is Associated with Estrogen Receptor Expression in Breast Tumors, but is not Under Direct Regulation by Estradiol, 5α-androstane-3β, 17β-Diol, or Dihydrotestosterone in Breast Cancer Cells
    Article Snippet: Cells were collected and RNA was extracted using RNeasy kit (Qiagen). cDNA was synthesized using Superscript III Reverse Transcriptase kit (Invitrogen). .. PCR was performed under the following conditions: 94°C for 3 minutes, followed by 30 cycles of 94°C for 30 seconds, 62°C for 1 minute, 72°C for 1.5 minutes, and then 72°C for 5 minutes.

    Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice
    Article Snippet: Briefly, total RNAs were transcribed into cDNAs by the SuperScript III Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA). .. The ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) as well as 2× PCR Master Mix were used to perform the qRT-PCR.

    Article Title: Salivary Scavenger and Agglutinin (SALSA) Is Expressed in Mucosal Epithelial Cells and Decreased in Bronchial Epithelium of Asthmatic Horses
    Article Snippet: .. Polymerase Chain Reaction for Whole Gene Sequencing RNA extracted from bronchial endoscopic biopsies was reversed transcribed to cDNA using a Superscript III Reverse Transcriptase kit (Invitrogen). ..

    Article Title: Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering
    Article Snippet: Paragraph title: RNA extraction and reverse transcriptase PCR (RT-PCR) ... The SuperScript III Reverse Transcriptase kit (Invitrogen) was used for RT-PCR.

    Plasmid Preparation:

    Article Title: Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering
    Article Snippet: RNA extraction and reverse transcriptase PCR (RT-PCR) To check expression of the cas9 gene under the lac promoter, total RNA of R. sphaeroides conjugated with the pBBR_Cas9_NT plasmid was extracted employing the RNAeasy mini kit (Qiagen, Germany) according to the manufacturer’s protocol and treated with DNAse I (NEB) to remove genomic DNA contamination in the sample. .. The SuperScript III Reverse Transcriptase kit (Invitrogen) was used for RT-PCR.

    Software:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: About 1 μg of total RNA was subjected to first-strand complementary DNA synthesis by using the SuperScript® III Reverse Transcriptase Kit (Invitrogen) as directed by the manufacturer. .. Primers (listed in ) were designed using Primer Express 2.0 software (Applied Biosystems, Foster City, CA).

    Real-time Polymerase Chain Reaction:

    Article Title: Salivary Scavenger and Agglutinin (SALSA) Is Expressed in Mucosal Epithelial Cells and Decreased in Bronchial Epithelium of Asthmatic Horses
    Article Snippet: Paragraph title: Quantitative PCR ... RNA was extracted from each endoscopic biopsy, and reverse transcribed into cDNA using a Superscript III Reverse Transcriptase kit (Invitrogen, Mississauga, ON, Canada) ( ).

    Article Title: ONC201 Targets AR and AR-V7 Signaling, Reduces PSA and Synergizes with Everolimus in Prostate Cancer
    Article Snippet: 1 μg of total RNA from each sample was subjected to cDNA synthesis using SuperScript® III Reverse Transcriptase kit (Life technologies, Grand Island, NY), for detection of the indicated genes and the housekeeping gene GAPDH. .. The relative expression of the reported genes was determined using real-time PCR performed using an Applied Biosystems 7900HT Fast Real-Time PCR system.

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Specific qPCR amplification was confirmed by obtaining a single distinct peak in melt curve analysis, and qPCR product sequencing. .. SuperScript III Reverse Transcriptase kit (Life Technologies) was used to synthesize the cDNA.

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative PCR analysis ... About 1 μg of total RNA was subjected to first-strand complementary DNA synthesis by using the SuperScript® III Reverse Transcriptase Kit (Invitrogen) as directed by the manufacturer.

    Article Title: lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-β pathway
    Article Snippet: Total RNA was transcribed into complementary DNA (cDNA) by RT using the SuperScript III Reverse Transcriptase kit (Thermo Fisher Scientific, Inc.). .. The reaction conditions were as follows: 50°C for 20 min and 80°C for 5 min. SYBR® Green Real-Time PCR Master mix (Thermo Fisher Scientific., Inc.) was used to prepare PCR reactions.

    Article Title: Inhibiting autophagy reduces retinal degeneration caused by protein misfolding
    Article Snippet: Paragraph title: Real-time polymerase chain reaction (RT-PCR) ... Total RNA (500 ng) was converted into cDNA using the SuperScript III Reverse Transcriptase Kit (ThermoFisher Scientific, 18,080,093).

    Article Title: Isorhamnetin Alleviates Steatosis and Fibrosis in Mice with Nonalcoholic Steatohepatitis
    Article Snippet: First-strand cDNA was amplified from the total RNA (100 ng) using SuperScript III Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA). .. Real-time quantitative PCR of target gene expression was assayed by TaqMan predesigned primers (Applied Biosystems, Foster City, CA, USA) and TaqMan Gene Expression Master Mix (Life Technologies, Carlsbad, USA) using the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Article Title: RETINOL SATURASE MODULATES LIPID METABOLISM AND THE PRODUCTION OF REACTIVE OXYGEN SPECIES
    Article Snippet: Complementary DNA (cDNA) was synthesized from 1 µg total RNA using SuperScript III reverse transcriptase kit (Invitrogen-Thermo Fisher Scientific) using oligo dT-primed reactions. .. Reactions were performed in a 10 µl volume of 25 ng/µl cDNA, 250 nM of each primer and 5 µl of QuantiFast SYBR Green PCR Master Mix (Qiagen, CA) on the Biosystems StepOnePlus™ Real-Time PCR System. qPCR condition was at 95 °C for 10 min, 95 °C for 10 sec. and 60 °C for 30 sec for 40 cycles.

    Article Title: A small set of conserved genes, including sp5 and Hox, are activated by Wnt signaling in the posterior of planarians and acoels
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) ... 1μg RNA input was used to prepare cDNA with the SuperScript III Reverse Transcriptase kit (Invitrogen).

    Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice
    Article Snippet: Briefly, total RNAs were transcribed into cDNAs by the SuperScript III Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA). .. The ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) as well as 2× PCR Master Mix were used to perform the qRT-PCR.

    RNA Extraction:

    Article Title: Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative PCR analysis ... About 1 μg of total RNA was subjected to first-strand complementary DNA synthesis by using the SuperScript® III Reverse Transcriptase Kit (Invitrogen) as directed by the manufacturer.

    Article Title: Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering
    Article Snippet: Paragraph title: RNA extraction and reverse transcriptase PCR (RT-PCR) ... The SuperScript III Reverse Transcriptase kit (Invitrogen) was used for RT-PCR.

    Selection:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Paragraph title: Candidate Gene Selection, Primer Design, and Gene Expression in Penultimate Leaves Under Control and Salinity ... SuperScript III Reverse Transcriptase kit (Life Technologies) was used to synthesize the cDNA.

    In Vitro:

    Article Title: lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-β pathway
    Article Snippet: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from tissues and in vitro cultivated cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. Total RNA was transcribed into complementary DNA (cDNA) by RT using the SuperScript III Reverse Transcriptase kit (Thermo Fisher Scientific, Inc.).

    Spectrophotometry:

    Article Title: Isorhamnetin Alleviates Steatosis and Fibrosis in Mice with Nonalcoholic Steatohepatitis
    Article Snippet: First-strand cDNA was amplified from the total RNA (100 ng) using SuperScript III Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA). .. The quantification of total RNA and cDNA was measured on NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    BAC Assay:

    Article Title: Salivary Scavenger and Agglutinin (SALSA) Is Expressed in Mucosal Epithelial Cells and Decreased in Bronchial Epithelium of Asthmatic Horses
    Article Snippet: RNA was extracted from each endoscopic biopsy, and reverse transcribed into cDNA using a Superscript III Reverse Transcriptase kit (Invitrogen, Mississauga, ON, Canada) ( ). .. Reference genes were selected from a pool of five commonly used reference gene candidates that had been previously evaluated in equine samples: beta-actin (BAC ), ribosomal protein L32 (RPL32 ), zeta polypeptide (YWHAZ ), succinate dehydrogenase complex subunit A (SDHA ), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) ( , ).

    Standard Deviation:

    Article Title: A small set of conserved genes, including sp5 and Hox, are activated by Wnt signaling in the posterior of planarians and acoels
    Article Snippet: 1μg RNA input was used to prepare cDNA with the SuperScript III Reverse Transcriptase kit (Invitrogen). .. Bar graph shows relative expression values as 2-ΔΔCT with standard deviation and individual expression values.

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    Thermo Fisher superscript iii first strand synthesis system
    Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii reverse transcriptase
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 48 article reviews
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