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    Qiagen superscript iii reverse transcriptase kit
    Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total <t>RNA</t> of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least <t>three</t> experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P
    Superscript Iii Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "TLR3 Modulates the Response of NK Cells against Schistosoma japonicum"

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

    Journal: Journal of Immunology Research

    doi: 10.1155/2018/7519856

    Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P
    Figure Legend Snippet: Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P

    Techniques Used: Expressing, Infection, Mouse Assay, Isolation, Flow Cytometry, Cytometry, FACS, Real-time Polymerase Chain Reaction

    2) Product Images from "TLR3 Modulates the Response of NK Cells against Schistosoma japonicum"

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

    Journal: Journal of Immunology Research

    doi: 10.1155/2018/7519856

    Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P
    Figure Legend Snippet: Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P

    Techniques Used: Expressing, Infection, Mouse Assay, Isolation, Flow Cytometry, Cytometry, FACS, Real-time Polymerase Chain Reaction

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    RNA Extraction:

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    Amplification:

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    RNA Sequencing Assay:

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    Synthesized:

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    Isolation:

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    Quantitative RT-PCR:

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    Mouse Assay:

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    Real-time Polymerase Chain Reaction:

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    Polymerase Chain Reaction:

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    Article Title: Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p. Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p
    Article Snippet: 2.4 Quantitative real‐time PCR RNA was extracted from cells via TRIzol reagent (Invitrogen, Carlsbad, California), then cDNA was synthesized with SuperScript III reverse transcriptase kit (Qiagen, Valencia, California) according to the instruction from the manufacturer. .. The qRT‐PCR was performed with TaqMan Universal PCR master mix (Applied Biosystems, Foster City, California) as described previously.

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    Article Snippet: RNA was extracted from cells via TRIzol reagent (Invitrogen, Carlsbad, California), then cDNA was synthesized with SuperScript III reverse transcriptase kit (Qiagen, Valencia, California) according to the instruction from the manufacturer. .. The qRT‐PCR was performed with TaqMan Universal PCR master mix (Applied Biosystems, Foster City, California) as described previously.

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    Article Snippet: 1 μ g of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. PCR conditions were listed as follows: 95°C for 30 s, followed by 40 cycles of 95°C for 5 s and 60°C for 30 s. Amplification was carried out in triplicate. β -Actin mRNA was used for normalization.

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum
    Article Snippet: 1 μ g of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. PCR conditions were listed as follows: 95°C for 30 s, followed by 40 cycles of 95°C for 5 s and 60°C for 30 s. Amplification was carried out in triplicate. β -Actin mRNA was used for normalization.

    Article Title: Notch and Wnt Signaling Cooperate in Regulation of Dendritic Cell Differentiation
    Article Snippet: RNA was extracted with an RNase Mini kit and cDNA was synthesized using SuperScript III Reverse Transcriptase kit (QIAGEN, Valencia, CA). .. PCR was performed as described earlier ( ) with 2.5 μl cDNA, 12.5 μl SYBR Master Mixture (Applied Biosystems, Foster City, CA), and target gene-specific primers ( ).

    Incubation:

    Article Title: Effect of glucocorticoids on expression of cutaneous antimicrobial peptides in northern leopard frogs (Lithobates pipiens)
    Article Snippet: Samples were treated with DNase I Incubation Mix (QIAGEN, Mississauga, Ontario), and RNA yield and quality were analyzed by spectrophotometry at 260 and 280 nm (NanoDrop, Thermo Scientific, Wilmington, Delaware). .. The first-strand cDNA was synthesized using the Superscript III Reverse Transcriptase Kit (QIAGEN, Mississauga, Ontario).

    Article Title: Expression of TLR2, TLR3, TLR4, and TLR7 on pulmonary lymphocytes ofSchistosoma japonicum-infected C57BL/6 mice
    Article Snippet: 1 μg of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. Reaction mixtures were incubated for 95°C for 30 s, followed by 95°C for 5s and 60°C for 30s (40 cycles) .

    Infection:

    Article Title: Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver
    Article Snippet: RNA preparation for RT-PCR RNA was extracted from the hepatic lymphocytes of infected and normal mice with Trizol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions. .. The cDNA was synthesized with a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA).

    Article Title: USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication
    Article Snippet: RNA isolation and real-time PCR (qRT-PCR) Cellular viral DNA/RNA was isolated from the CD4 T-cells of HIV-1 infected individuals according to the instructions using AllPure DNA/RNA Micro Kit (Magen, R5112-02). .. Then cDNA was synthesized using a SuperScript III reverse transcriptase kit (Qiagen, Valencia, CA).

    Expressing:

    Article Title: ciaR impacts biofilm formation by regulating an arginine biosynthesis pathway in Streptococcus sanguinis SK36
    Article Snippet: Reverse transcription followed the standard procedure provided with the SuperScript™ III Reverse Transcriptase Kit (Qiagen). .. Gene expression in ΔciaR is relative to that in WT.

    Article Title: Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p. Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p
    Article Snippet: 2.4 Quantitative real‐time PCR RNA was extracted from cells via TRIzol reagent (Invitrogen, Carlsbad, California), then cDNA was synthesized with SuperScript III reverse transcriptase kit (Qiagen, Valencia, California) according to the instruction from the manufacturer. .. Relative mRNA and lncRNA expression were calculated with normalization to GAPDH, and U6 was used to normalize expression of miRNA.

    Article Title: Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p. Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p
    Article Snippet: RNA was extracted from cells via TRIzol reagent (Invitrogen, Carlsbad, California), then cDNA was synthesized with SuperScript III reverse transcriptase kit (Qiagen, Valencia, California) according to the instruction from the manufacturer. .. Relative mRNA and lncRNA expression were calculated with normalization to GAPDH, and U6 was used to normalize expression of miRNA.

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum
    Article Snippet: 1 μ g of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. The following primers were synthesized by Invitrogen (Shanghai, China): for TLR2, 5-CTCTCCGTCCCAACTGATGA-3 (forward) and 5-GGTCTGGTTGCATGGCTTTT-3 (reverse); for TLR3, 5-ATTCGCCCTCCTCTTGAACA-3 (forward) and 5-TCGAGCTGGGTGAGATTTGT-3 (reverse); for TL4, 5-AGGTTGAGAAGTCCCTGCTG-3 (forward) and 5-GGTCCAAGTTGCCGTTTCTT-3 (reverse); for TLR7, 5-GCATTCCCACTAACACCACC-3 (forward) and 5-ACACACATTGGCTTTGGACC-3 (reverse); for β -actin, 5-CCGTAAAGACCTCTATGCCAAC-3 (forward) and 5-GGGTGTAAAACGCAGCTCAGTA-3 (reverse). mRNA expression was analyzed with RT-qPCR by using Takara SYBR Premix Ex Taq II (RR820A).

    Article Title: Effect of glucocorticoids on expression of cutaneous antimicrobial peptides in northern leopard frogs (Lithobates pipiens)
    Article Snippet: Paragraph title: Measurement of antimicrobial peptide gene expression ... The first-strand cDNA was synthesized using the Superscript III Reverse Transcriptase Kit (QIAGEN, Mississauga, Ontario).

    Article Title: Expression of TLR2, TLR3, TLR4, and TLR7 on pulmonary lymphocytes ofSchistosoma japonicum-infected C57BL/6 mice
    Article Snippet: 1 μg of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. Primers were synthesized by Invitrogen (Shanghai, China) and were TLR2 sense 5-CTCTCCGTCCCAACTGATGA-3, antisense 5-GGTCTGGTTGCATGGCTTTT-3; TLR3 sense 5-ATTCGCCCTCCTCTTGAACA-3, antisense 5-TCGAGCTGGGTGAGATTTGT-3; TL4 sense 5-AGGTTGAGAAGTCCCTGCTG-3, antisense 5-GGTCCAAGTTGCCGTTTCTT-3; TLR7 sense 5-GCATTCCCACTAACACCACC-3, antisense 5-ACACACATTGGCTTTGGACC-3 (reverse); β-antisense 5-CCGTAAAGACCTCTATGCCAAC-3, antisense 5-GGGTGTAAAACGCAGCTCAGTA-3. mRNA expression was analyzed with RT-qPCR by using Takara SYBR Premix Ex Taq II (RR820A).

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum
    Article Snippet: 1 μ g of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. The following primers were synthesized by Invitrogen (Shanghai, China): for TLR2, 5-CTCTCCGTCCCAACTGATGA-3 (forward) and 5-GGTCTGGTTGCATGGCTTTT-3 (reverse); for TLR3, 5-ATTCGCCCTCCTCTTGAACA-3 (forward) and 5-TCGAGCTGGGTGAGATTTGT-3 (reverse); for TL4, 5-AGGTTGAGAAGTCCCTGCTG-3 (forward) and 5-GGTCCAAGTTGCCGTTTCTT-3 (reverse); for TLR7, 5-GCATTCCCACTAACACCACC-3 (forward) and 5-ACACACATTGGCTTTGGACC-3 (reverse); for β -actin, 5-CCGTAAAGACCTCTATGCCAAC-3 (forward) and 5-GGGTGTAAAACGCAGCTCAGTA-3 (reverse). mRNA expression was analyzed with RT-qPCR by using Takara SYBR Premix Ex Taq II (RR820A).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver
    Article Snippet: Paragraph title: RNA preparation for RT-PCR ... The cDNA was synthesized with a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA).

    Western Blot:

    Article Title: Notch and Wnt Signaling Cooperate in Regulation of Dendritic Cell Differentiation
    Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting ... RNA was extracted with an RNase Mini kit and cDNA was synthesized using SuperScript III Reverse Transcriptase kit (QIAGEN, Valencia, CA).

    Spectrophotometry:

    Article Title: Effect of glucocorticoids on expression of cutaneous antimicrobial peptides in northern leopard frogs (Lithobates pipiens)
    Article Snippet: Samples were treated with DNase I Incubation Mix (QIAGEN, Mississauga, Ontario), and RNA yield and quality were analyzed by spectrophotometry at 260 and 280 nm (NanoDrop, Thermo Scientific, Wilmington, Delaware). .. The first-strand cDNA was synthesized using the Superscript III Reverse Transcriptase Kit (QIAGEN, Mississauga, Ontario).

    Agarose Gel Electrophoresis:

    Article Title: Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver
    Article Snippet: The cDNA was synthesized with a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA). .. Q-PCR products were analysed on a 1.0% multi-welled agarose gel.

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    Qiagen superscript iii reverse transcriptase kit
    Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total <t>RNA</t> of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least <t>three</t> experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P
    Superscript Iii Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P

    Journal: Journal of Immunology Research

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

    doi: 10.1155/2018/7519856

    Figure Lengend Snippet: Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P

    Article Snippet: 1 μ g of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA).

    Techniques: Expressing, Infection, Mouse Assay, Isolation, Flow Cytometry, Cytometry, FACS, Real-time Polymerase Chain Reaction

    Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P

    Journal: Journal of Immunology Research

    Article Title: TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

    doi: 10.1155/2018/7519856

    Figure Lengend Snippet: Expression of different TLRs in mouse splenic lymphocytes and NK cells after S. japonicum infection. (a) Splenic lymphocytes were separated from normal and S. japonicum -infected wild-type mice, and CD3 − NK1.1 + NK cells were isolated from splenic lymphocytes by using flow cytometry and the purity of isolated splenic NK cells was identified by FACS. (b) Total RNA of splenic lymphocytes and NK cells was harvested, respectively. The accumulation of TLR2, TLR3, TLR4, and TLR7 mRNA was quantified by using qPCR. The levels of TLR transcripts were normalized to β -actin transcripts by using the relative quantity (RQ) = 2 −△△Ct method. Data represent means ± SEM of at least three experiments. (c, d) Expression of TLR2, TLR3, TLR4, and TLR7 on splenic NK cells was assessed by using flow cytometry. (c) A representative result is shown. (d) Statistic results of 6 to 8 independent results are shown. N: normal; INF: infected; ∗ P

    Article Snippet: 1 μ g of total RNA was transcribed to cDNA by using a SuperScript III Reverse Transcriptase Kit (Qiagen, Valencia, CA).

    Techniques: Expressing, Infection, Mouse Assay, Isolation, Flow Cytometry, Cytometry, FACS, Real-time Polymerase Chain Reaction