superscript iii first strand cdna synthesis kit  (Thermo Fisher)


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    Name:
    First Strand cDNA Synthesis Kit
    Description:
    Thermo Scientific First Strand cDNA Synthesis kit utilizes the recombinant M MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase Due to this feature full length cDNA can be synthesized from RNA templates up to 9 kb The recombinant RiboLock RNase Inhibitor supplied with the kit effectively protects RNA template from degradation The first strand of cDNA can be directly used as a template in PCR or in second strand cDNA synthesis Highlights• Efficient synthesis of first strand cDNA up to 9 kb• Reaction temperature 37°C• Supplied with the recombinant RiboLock RNase Inhibitor• Complete oligo dT 18 and random hexamer primers included with the kitApplications• First strand cDNA synthesis for RT PCR and real time RT PCR• Construction of cDNA libraries• Generation of probes for hybridization• aRNA synthesisThe kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 anneals selectively on the poly A tail of mRNA Random hexamer primers do not require the presence of poly A Therefore they can be used for transcription of the 5 end regions of mRNA or cDNA synthesis using RNA without poly A tail e g micro RNAs Gene specific primers may also be used with the kits
    Catalog Number:
    k1612
    Price:
    None
    Category:
    Kits and Assays
    Applications:
    Cloning|cDNA Libraries & Library Construction
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    Structured Review

    Thermo Fisher superscript iii first strand cdna synthesis kit
    Thermo Scientific First Strand cDNA Synthesis kit utilizes the recombinant M MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase Due to this feature full length cDNA can be synthesized from RNA templates up to 9 kb The recombinant RiboLock RNase Inhibitor supplied with the kit effectively protects RNA template from degradation The first strand of cDNA can be directly used as a template in PCR or in second strand cDNA synthesis Highlights• Efficient synthesis of first strand cDNA up to 9 kb• Reaction temperature 37°C• Supplied with the recombinant RiboLock RNase Inhibitor• Complete oligo dT 18 and random hexamer primers included with the kitApplications• First strand cDNA synthesis for RT PCR and real time RT PCR• Construction of cDNA libraries• Generation of probes for hybridization• aRNA synthesisThe kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 anneals selectively on the poly A tail of mRNA Random hexamer primers do not require the presence of poly A Therefore they can be used for transcription of the 5 end regions of mRNA or cDNA synthesis using RNA without poly A tail e g micro RNAs Gene specific primers may also be used with the kits
    https://www.bioz.com/result/superscript iii first strand cdna synthesis kit/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript iii first strand cdna synthesis kit - by Bioz Stars, 2021-03
    86/100 stars

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    Generated:

    Article Title: Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation
    Article Snippet: Poly(A)-enriched RNAs were captured with oligo(dT) Dynabeads (Thermo Fisher Scientific) according to manufacturer’s instructions and fragmented in fragmentation buffer (50 mM Tris acetate pH 8.1, 100 mM KOAc, 30 mM MgOA) for 5 mins at 80 °C. .. First-strand cDNA was generated using SuperScript III (Invitrogen) and random primers following manufacturer’s instructions. .. Second-strand cDNA was generated with the BioNotI-P5-PET oligo and using Phusion High-Fidelity Polymerase (NEB) as per manufacturer’s instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Muscular tissues of the squid Doryteuthis pealeii express identical myosin heavy chain isoforms: an alternative mechanism for tuning contractile speed
    Article Snippet: Total RNA was extracted from 30–50 mg of each tissue using the GeneJET RNA Purification Kit (Fermentas, Glen Burnie, MD, USA) and treated with DNase I (Fermentas). .. First strand cDNA synthesis was performed using 125–250 ng total RNA with the Moloney murine leukemia virus reverse transcriptase (M-MuLV RT) from the Phusion RT-PCR Kit according to the manufacturer's instructions (Finnzymes, Espoo, Finland). .. An oligo(dT)15 primer was used to synthesize cDNA for conventional PCR, an anchor primer (Q_total) was used for 3′ rapid amplification of cDNA ends (RACE) cDNA synthesis and a myosin-specific primer (MHC_RT) was used for 5′ RACE cDNA synthesis ( ).

    Amplification:

    Article Title: Metatranscriptomic and functional metagenomic analysis of methylphosphonate utilization by marine bacteria
    Article Snippet: RNA amplification and cDNA synthesis were performed as described previously (McCarren et al., ; Shi et al., ). .. In brief, roughly 10–20 ng of total RNA were amplified using MessageAmp II Bacteria kit (Ambion) and T7Bpm IdT16 VN, an oligo(dT) primer containing a promoter sequence for T7 RNA polymerase and a Bpm I recognition site. cDNA was synthesized from the amplified RNA using Superscript III (Invitrogen) and random hexamers for first-strand complementary DNA synthesis, and the SuperScript Double Stranded cDNA Synthesis kit (Invitrogen) for second-strand synthesis. .. The resulting cDNA was purified using the Qiaquick PCR purification kit (Qiagen), digested with Bpm I to remove poly(A) tails and used directly for pyrosequencing. gDNA and cDNA single-stranded libraries were prepared and sequenced using standard GS FLX protocols (454 Life Sciences, Roche) in a Roche Genome Sequencer FLX (Roche Applied Science, Indianapolis, IN).

    Sequencing:

    Article Title: Metatranscriptomic and functional metagenomic analysis of methylphosphonate utilization by marine bacteria
    Article Snippet: RNA amplification and cDNA synthesis were performed as described previously (McCarren et al., ; Shi et al., ). .. In brief, roughly 10–20 ng of total RNA were amplified using MessageAmp II Bacteria kit (Ambion) and T7Bpm IdT16 VN, an oligo(dT) primer containing a promoter sequence for T7 RNA polymerase and a Bpm I recognition site. cDNA was synthesized from the amplified RNA using Superscript III (Invitrogen) and random hexamers for first-strand complementary DNA synthesis, and the SuperScript Double Stranded cDNA Synthesis kit (Invitrogen) for second-strand synthesis. .. The resulting cDNA was purified using the Qiaquick PCR purification kit (Qiagen), digested with Bpm I to remove poly(A) tails and used directly for pyrosequencing. gDNA and cDNA single-stranded libraries were prepared and sequenced using standard GS FLX protocols (454 Life Sciences, Roche) in a Roche Genome Sequencer FLX (Roche Applied Science, Indianapolis, IN).

    Synthesized:

    Article Title: Metatranscriptomic and functional metagenomic analysis of methylphosphonate utilization by marine bacteria
    Article Snippet: RNA amplification and cDNA synthesis were performed as described previously (McCarren et al., ; Shi et al., ). .. In brief, roughly 10–20 ng of total RNA were amplified using MessageAmp II Bacteria kit (Ambion) and T7Bpm IdT16 VN, an oligo(dT) primer containing a promoter sequence for T7 RNA polymerase and a Bpm I recognition site. cDNA was synthesized from the amplified RNA using Superscript III (Invitrogen) and random hexamers for first-strand complementary DNA synthesis, and the SuperScript Double Stranded cDNA Synthesis kit (Invitrogen) for second-strand synthesis. .. The resulting cDNA was purified using the Qiaquick PCR purification kit (Qiagen), digested with Bpm I to remove poly(A) tails and used directly for pyrosequencing. gDNA and cDNA single-stranded libraries were prepared and sequenced using standard GS FLX protocols (454 Life Sciences, Roche) in a Roche Genome Sequencer FLX (Roche Applied Science, Indianapolis, IN).

    Article Title: DNA Cytosine Methylation in the Bovine Leukemia Virus Promoter Is Associated with Latency in a Lymphoma-derived B-cell Line
    Article Snippet: Total RNA samples were extracted from 1 × 106 cells using the RNeasy Plus mini kit (Qiagen) according to the manufacturer's instructions and digested with TURBO DNase I (TURBO DNA-freeTM kit, Ambion) to ensure the removal of genomic DNA. .. First strand cDNA was synthesized using SuperScript III reverse transcriptase (Invitrogen). .. Quantitative real-time PCR (qPCR) reactions were performed using MESA GREEN qPCR MasterMix (Eurogentec), and 96-well optical reaction plates were read in an Applied Biosystems StepOnePlus real-time PCR system (comparative Ct (ΔΔCt ) quantification method).

    DNA Synthesis:

    Article Title: Metatranscriptomic and functional metagenomic analysis of methylphosphonate utilization by marine bacteria
    Article Snippet: RNA amplification and cDNA synthesis were performed as described previously (McCarren et al., ; Shi et al., ). .. In brief, roughly 10–20 ng of total RNA were amplified using MessageAmp II Bacteria kit (Ambion) and T7Bpm IdT16 VN, an oligo(dT) primer containing a promoter sequence for T7 RNA polymerase and a Bpm I recognition site. cDNA was synthesized from the amplified RNA using Superscript III (Invitrogen) and random hexamers for first-strand complementary DNA synthesis, and the SuperScript Double Stranded cDNA Synthesis kit (Invitrogen) for second-strand synthesis. .. The resulting cDNA was purified using the Qiaquick PCR purification kit (Qiagen), digested with Bpm I to remove poly(A) tails and used directly for pyrosequencing. gDNA and cDNA single-stranded libraries were prepared and sequenced using standard GS FLX protocols (454 Life Sciences, Roche) in a Roche Genome Sequencer FLX (Roche Applied Science, Indianapolis, IN).

    Quantitative RT-PCR:

    Article Title: Tumour-suppressive microRNA-29s inhibit cancer cell migration and invasion by targeting laminin–integrin signalling in head and neck squamous cell carcinoma
    Article Snippet: Both cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum in a humidified atmosphere containing 5% CO2 at 37 °C. .. Quantitative real-time RT–PCR (qRT–PCR) First-strand cDNA was synthesised from 1 μ g of total RNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. Gene-specific PCR products were assayed continuously using a 7900-HT Real-Time PCR System according to the manufacturer's protocol.

    Article Title: LRIG1 is a pleiotropic androgen receptor-regulated feedback tumor suppressor in prostate cancer
    Article Snippet: RNA isolation and quantitative RT-PCR analysis Total RNA was isolated from cells, mouse prostates or human xenograft tumors using RNeasy mini kit (Qiagen) following the manufacturer’s protocol. .. For qRT-PCR, first-strand cDNA synthesis from total RNA was carried out using SuperScript® III Frist-Strand synthesis kit (Life Technology), and the resulting cDNA was then incubated with iTaq Universal SYBR Green Supermix (BIO-RAD) and the respective mRNA levels were analyzed by qRT-PCR in ABI Prism 7900HT Real-Time PCR detection system by normalizing to human GAPDH or mouse Gapdh. qRT-PCR primers were listed in Supplementary Table . .. Western blotting and quantitative Wes immunoassays Whole cell lysates (WCL) from cells or tumor tissues were prepared in complete RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.5% Triton X-100) containing protease inhibitor mixture, and the protein concentrations were measured via MicroBCA kit (Pierce).

    Incubation:

    Article Title: LRIG1 is a pleiotropic androgen receptor-regulated feedback tumor suppressor in prostate cancer
    Article Snippet: RNA isolation and quantitative RT-PCR analysis Total RNA was isolated from cells, mouse prostates or human xenograft tumors using RNeasy mini kit (Qiagen) following the manufacturer’s protocol. .. For qRT-PCR, first-strand cDNA synthesis from total RNA was carried out using SuperScript® III Frist-Strand synthesis kit (Life Technology), and the resulting cDNA was then incubated with iTaq Universal SYBR Green Supermix (BIO-RAD) and the respective mRNA levels were analyzed by qRT-PCR in ABI Prism 7900HT Real-Time PCR detection system by normalizing to human GAPDH or mouse Gapdh. qRT-PCR primers were listed in Supplementary Table . .. Western blotting and quantitative Wes immunoassays Whole cell lysates (WCL) from cells or tumor tissues were prepared in complete RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.5% Triton X-100) containing protease inhibitor mixture, and the protein concentrations were measured via MicroBCA kit (Pierce).

    SYBR Green Assay:

    Article Title: LRIG1 is a pleiotropic androgen receptor-regulated feedback tumor suppressor in prostate cancer
    Article Snippet: RNA isolation and quantitative RT-PCR analysis Total RNA was isolated from cells, mouse prostates or human xenograft tumors using RNeasy mini kit (Qiagen) following the manufacturer’s protocol. .. For qRT-PCR, first-strand cDNA synthesis from total RNA was carried out using SuperScript® III Frist-Strand synthesis kit (Life Technology), and the resulting cDNA was then incubated with iTaq Universal SYBR Green Supermix (BIO-RAD) and the respective mRNA levels were analyzed by qRT-PCR in ABI Prism 7900HT Real-Time PCR detection system by normalizing to human GAPDH or mouse Gapdh. qRT-PCR primers were listed in Supplementary Table . .. Western blotting and quantitative Wes immunoassays Whole cell lysates (WCL) from cells or tumor tissues were prepared in complete RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.5% Triton X-100) containing protease inhibitor mixture, and the protein concentrations were measured via MicroBCA kit (Pierce).

    Real-time Polymerase Chain Reaction:

    Article Title: LRIG1 is a pleiotropic androgen receptor-regulated feedback tumor suppressor in prostate cancer
    Article Snippet: RNA isolation and quantitative RT-PCR analysis Total RNA was isolated from cells, mouse prostates or human xenograft tumors using RNeasy mini kit (Qiagen) following the manufacturer’s protocol. .. For qRT-PCR, first-strand cDNA synthesis from total RNA was carried out using SuperScript® III Frist-Strand synthesis kit (Life Technology), and the resulting cDNA was then incubated with iTaq Universal SYBR Green Supermix (BIO-RAD) and the respective mRNA levels were analyzed by qRT-PCR in ABI Prism 7900HT Real-Time PCR detection system by normalizing to human GAPDH or mouse Gapdh. qRT-PCR primers were listed in Supplementary Table . .. Western blotting and quantitative Wes immunoassays Whole cell lysates (WCL) from cells or tumor tissues were prepared in complete RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.5% Triton X-100) containing protease inhibitor mixture, and the protein concentrations were measured via MicroBCA kit (Pierce).

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  • 97
    Thermo Fisher superscript iii kit
    STIM1 depletion reduces ER Ca 2+ content in T cells. ( A ) Expression of STIM1 ( Left ) and Orai1 ( Right ) in control and JP4-depleted Jurkat cells detected by immunoblotting and quantitative <t>RT-PCR,</t> respectively. The transcript data show mean ± SEM of triplicates. ( B ) STIM1 expression in control (Scr) or STIM1-depleted (STIM1 KD) Jurkat cells determined by immunoblotting. β-Actin was used as a loading control. ( C ) SOCE and ER Ca 2+ content measurements in control (Scr) or STIM1-depleted (STIM1 KD) Jurkat cells after passive store depletion with thapsigargin (TG) (1 μM) in Ca 2+ -free solution, and addition of 2 mM Ca 2+ -containing solution ( Left ). Traces show averaged (±SEM) responses from 30 to 50 Jurkat T cells. Bar graphs show change in ER Ca 2+ content and SOCE (±SEM) from <t>three</t> independent experiments. Control (Scr) and STIM1-depleted (KD) Jurkat cells were treated with ionomycin (1 μM, iono) in Ca 2+ -free solution to measure the ER Ca 2+ content ( Right two panels). Traces show averaged (±SEM) responses from 30 to 50 cells, and bar graph shows change in ER Ca 2+ content (±SEM) from three independent experiments. * P
    Superscript Iii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii kit/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript iii kit - by Bioz Stars, 2021-03
    97/100 stars
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    STIM1 depletion reduces ER Ca 2+ content in T cells. ( A ) Expression of STIM1 ( Left ) and Orai1 ( Right ) in control and JP4-depleted Jurkat cells detected by immunoblotting and quantitative RT-PCR, respectively. The transcript data show mean ± SEM of triplicates. ( B ) STIM1 expression in control (Scr) or STIM1-depleted (STIM1 KD) Jurkat cells determined by immunoblotting. β-Actin was used as a loading control. ( C ) SOCE and ER Ca 2+ content measurements in control (Scr) or STIM1-depleted (STIM1 KD) Jurkat cells after passive store depletion with thapsigargin (TG) (1 μM) in Ca 2+ -free solution, and addition of 2 mM Ca 2+ -containing solution ( Left ). Traces show averaged (±SEM) responses from 30 to 50 Jurkat T cells. Bar graphs show change in ER Ca 2+ content and SOCE (±SEM) from three independent experiments. Control (Scr) and STIM1-depleted (KD) Jurkat cells were treated with ionomycin (1 μM, iono) in Ca 2+ -free solution to measure the ER Ca 2+ content ( Right two panels). Traces show averaged (±SEM) responses from 30 to 50 cells, and bar graph shows change in ER Ca 2+ content (±SEM) from three independent experiments. * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Junctophilin-4, a component of the endoplasmic reticulum–plasma membrane junctions, regulates Ca2+ dynamics in T cells

    doi: 10.1073/pnas.1524229113

    Figure Lengend Snippet: STIM1 depletion reduces ER Ca 2+ content in T cells. ( A ) Expression of STIM1 ( Left ) and Orai1 ( Right ) in control and JP4-depleted Jurkat cells detected by immunoblotting and quantitative RT-PCR, respectively. The transcript data show mean ± SEM of triplicates. ( B ) STIM1 expression in control (Scr) or STIM1-depleted (STIM1 KD) Jurkat cells determined by immunoblotting. β-Actin was used as a loading control. ( C ) SOCE and ER Ca 2+ content measurements in control (Scr) or STIM1-depleted (STIM1 KD) Jurkat cells after passive store depletion with thapsigargin (TG) (1 μM) in Ca 2+ -free solution, and addition of 2 mM Ca 2+ -containing solution ( Left ). Traces show averaged (±SEM) responses from 30 to 50 Jurkat T cells. Bar graphs show change in ER Ca 2+ content and SOCE (±SEM) from three independent experiments. Control (Scr) and STIM1-depleted (KD) Jurkat cells were treated with ionomycin (1 μM, iono) in Ca 2+ -free solution to measure the ER Ca 2+ content ( Right two panels). Traces show averaged (±SEM) responses from 30 to 50 cells, and bar graph shows change in ER Ca 2+ content (±SEM) from three independent experiments. * P

    Article Snippet: For RT-PCR, purified RNA was oligo(dT)-primed for first-strand cDNA synthesis (Superscript III kit; Invitrogen).

    Techniques: Expressing, Quantitative RT-PCR

    DRAIC knockout increases tumorigenic property of prostate cancer cells through NF-κB pathway. (A) A schematic illustration of DRAIC knockout strategy using CRISPR/Cas9 in LNCaP cells is shown. The sgRNAs were designed from DRAIC exon 2 and exon 4 as indicated by the arrowhead to knockout Exon 2–4. (B) Three representative single clones with KO of DRAIC identified by truncated PCR product from DRAIC genomic DNA. M: DNA molecular weight marker. (C) RT-qPCR of DRAIC RNA from WT and KO clones. Mean±s.d, n=4, **p

    Journal: Cancer research

    Article Title: Long noncoding RNA DRAIC inhibits prostate cancer progression by interacting with IKK to inhibit NF-κB activation

    doi: 10.1158/0008-5472.CAN-19-3460

    Figure Lengend Snippet: DRAIC knockout increases tumorigenic property of prostate cancer cells through NF-κB pathway. (A) A schematic illustration of DRAIC knockout strategy using CRISPR/Cas9 in LNCaP cells is shown. The sgRNAs were designed from DRAIC exon 2 and exon 4 as indicated by the arrowhead to knockout Exon 2–4. (B) Three representative single clones with KO of DRAIC identified by truncated PCR product from DRAIC genomic DNA. M: DNA molecular weight marker. (C) RT-qPCR of DRAIC RNA from WT and KO clones. Mean±s.d, n=4, **p

    Article Snippet: 1 μg of total RNA was reverse transcribed using SuperScript III First-Strand cDNA synthesis kit (Thermo Fisher Scientific, catalog no. 18080051).

    Techniques: Knock-Out, CRISPR, Clone Assay, Polymerase Chain Reaction, Molecular Weight, Marker, Quantitative RT-PCR

    Role of Interferon Signaling in VRP-Induced Host Gene Expression in Infected versus Bystander BMDC BMDC (10 6 ) generated from wildtype 129sv/ev mice (black bars) were infected with FLAG-PABP VRP at a low MOI (0.5). At 6 h, RNA was isolated from mock and VRP-infected BMDC by either 1) preparing cell lysates for isolation of FLAG-PABP-bound mRNA via anti-FLAG immunoprecipitation, or 2) using UltraSpec reagent to isolate total cellular RNA. The mRNP-tagging method specifically isolated mRNA from the minority of infected BMDC via the bound FLAG-PABP. Conversely, total RNA extraction was used to isolate cellular RNA from the entire VRP-infected BMDC culture, with the majority of the population being DCs that had not been infected. To examine the contribution of signaling through the IFNαβ receptor, the same analysis was carried out in BMDC derived from IFNαβR−/− mice (hatched bars). cDNA was generated from each RNA isolation, and assessed for changes in host gene expression by Taqman real-time PCR. Three independent samples were normalized to GAPDH signal and analyzed in comparison to mock infected BMDC: Infected cell anti-FLAG(PABP) signal was compared to mock PABP signal, and infected total RNA signal was compared to mock total RNA signal. The data are shown as the geometric mean, ± the standard error of the mean.

    Journal: PLoS Pathogens

    Article Title: A Two-Phase Innate Host Response to Alphavirus Infection Identified by mRNP-Tagging In Vivo

    doi: 10.1371/journal.ppat.0030199

    Figure Lengend Snippet: Role of Interferon Signaling in VRP-Induced Host Gene Expression in Infected versus Bystander BMDC BMDC (10 6 ) generated from wildtype 129sv/ev mice (black bars) were infected with FLAG-PABP VRP at a low MOI (0.5). At 6 h, RNA was isolated from mock and VRP-infected BMDC by either 1) preparing cell lysates for isolation of FLAG-PABP-bound mRNA via anti-FLAG immunoprecipitation, or 2) using UltraSpec reagent to isolate total cellular RNA. The mRNP-tagging method specifically isolated mRNA from the minority of infected BMDC via the bound FLAG-PABP. Conversely, total RNA extraction was used to isolate cellular RNA from the entire VRP-infected BMDC culture, with the majority of the population being DCs that had not been infected. To examine the contribution of signaling through the IFNαβ receptor, the same analysis was carried out in BMDC derived from IFNαβR−/− mice (hatched bars). cDNA was generated from each RNA isolation, and assessed for changes in host gene expression by Taqman real-time PCR. Three independent samples were normalized to GAPDH signal and analyzed in comparison to mock infected BMDC: Infected cell anti-FLAG(PABP) signal was compared to mock PABP signal, and infected total RNA signal was compared to mock total RNA signal. The data are shown as the geometric mean, ± the standard error of the mean.

    Article Snippet: A one-tube DNase treatment and reverse transcription protocol was used to generate cDNA, using SuperScript III Reverse Transcriptase First Strand cDNA kit (Invitrogen).

    Techniques: Expressing, Infection, Generated, Mouse Assay, Isolation, Immunoprecipitation, RNA Extraction, Derivative Assay, Real-time Polymerase Chain Reaction

    The relative abundance of the various diazotroph clades. The cyanobacteria are labeled in shades of green, the Gammaproteobacteria are in blue, the Alphaproteobacteria are in red, the cluster-III diazotrophs are in yellow, and the Firmicutes are in purple. The abundance of the diazotroph clades are shown in (A) the surface and (B) DCM waters of all the stations, as well as (C) for two cDNA samples collected in the surface and DCM layers of ST.3.

    Journal: PLoS ONE

    Article Title: Highly heterogeneous diazotroph communities in the Kuroshio Current and the Tokara Strait, Japan

    doi: 10.1371/journal.pone.0186875

    Figure Lengend Snippet: The relative abundance of the various diazotroph clades. The cyanobacteria are labeled in shades of green, the Gammaproteobacteria are in blue, the Alphaproteobacteria are in red, the cluster-III diazotrophs are in yellow, and the Firmicutes are in purple. The abundance of the diazotroph clades are shown in (A) the surface and (B) DCM waters of all the stations, as well as (C) for two cDNA samples collected in the surface and DCM layers of ST.3.

    Article Snippet: For cDNA synthesis, the extracted RNA was first treated with DNase I to remove any contaminating DNA, after which it was reverse-transcribed with the SuperScript III First Strand cDNA Synthesis kit (Invitrogen, Thermo Fisher Scientific Corp.).

    Techniques: Labeling