superscript iii cdna kit  (Thermo Fisher)


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    Name:
    SuperScript Plus Direct cDNA Labeling Module no purification
    Description:
    The SuperScript Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript III Reverse Transcriptase RT with the simplicity of direct labeling In place of an enzyme spike in the streamlined protocol incorporates balanced sets of novel array optimized nucleotides conjugated to Alexa Fluor 555 and 647 dyes This results in more reproducible array data with higher correlation coefficients than using standalone reagents The SuperScript Plus Direct cDNA Labeling System provides • Optimized all inclusive direct labeling with balanced aha Alexa Fluor nucleotides enabling higher numbers of array positives• Improved correlation coefficients for more accurate data Figure 1 • Low elution purification columns included for ease of useIn the SuperScript Plus cDNA Labeling method anchored oligo dT anneals to the mRNA template Figure 2 SuperScript III RT extends from the priming site incorporating dUTP labeled with Alexa Fluor dye directly to produce labeled cDNA Template RNA is degraded by base hydrolysis Free nucleotides and other contaminants are removed using low elution volume purification columns before hybridization to the microarray
    Catalog Number:
    l101504
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Labeling|Nucleic Acid Labeling & Oligo Synthesis|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher superscript iii cdna kit
    The SuperScript Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript III Reverse Transcriptase RT with the simplicity of direct labeling In place of an enzyme spike in the streamlined protocol incorporates balanced sets of novel array optimized nucleotides conjugated to Alexa Fluor 555 and 647 dyes This results in more reproducible array data with higher correlation coefficients than using standalone reagents The SuperScript Plus Direct cDNA Labeling System provides • Optimized all inclusive direct labeling with balanced aha Alexa Fluor nucleotides enabling higher numbers of array positives• Improved correlation coefficients for more accurate data Figure 1 • Low elution purification columns included for ease of useIn the SuperScript Plus cDNA Labeling method anchored oligo dT anneals to the mRNA template Figure 2 SuperScript III RT extends from the priming site incorporating dUTP labeled with Alexa Fluor dye directly to produce labeled cDNA Template RNA is degraded by base hydrolysis Free nucleotides and other contaminants are removed using low elution volume purification columns before hybridization to the microarray
    https://www.bioz.com/result/superscript iii cdna kit/product/Thermo Fisher
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    superscript iii cdna kit - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Origin and Evolution of the Sponge Aggregation Factor Gene Family
    Article Snippet: .. After DNase I treatment (Invitrogen), cDNA was synthesized from RNA using SuperScript III reverse transcriptase (Invitrogen) and oligo(dT)15 and random pentadecamer primers , according to manufacturer’s guidelines. ..

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. RT-PCR Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Fusarium oxysporum Triggers Tissue-Specific Transcriptional Reprogramming in Arabidopsis thaliana
    Article Snippet: .. F . oxysporum RT-qPCR First-strand cDNA was synthesized from 1μg RNA using SuperScript RNA H- Reverse Transcriptase (Invitrogen) and oligo (dT) primer, according to the manufacturer's instructions. .. RT-qPCR products were amplified in 10μl reactions containing 2μl cDNA, 2x SYBR® Green PCR Master Mix (Applied Biosystems) and 0.3 pmol primers.

    Article Title: OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae
    Article Snippet: .. First-stand cDNA was synthesized from total RNA using the Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA). .. RT-qPCR was performed using SYBR Green detection reagents (Quanta Biosciences, Carlsbad, CA, USA) in Applied Biosystem’s 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) with the primers (catBqF/catBqR, oxyRqF/oxyRqR), and gyrB was used as a reference gene (Additional file : Table S2).

    Isolation:

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. RT-PCR Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Labeling:

    Article Title: Differential expression of MUC genes in endometrial and cervical tissues and tumors
    Article Snippet: .. The cDNA probes (MUC1 , MUC2 , MUC5B , MUC5AC , MUC8 and β-actin ) were labeled by the random priming technique using DECAprime II kit (Ambion, Austin, TX). .. Hybridization of 32 P-labeled cDNA probes was carried out at 42°C for 14–16 h. After hybridization, membranes were washed twice in 2× SSC and 0.1% SDS for 10 min. at room temperature, followed by two additional washes for 20 min at 65°C in 1× SSC and 0.1% SDS solution.

    Real-time Polymerase Chain Reaction:

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Generated:

    Article Title: Aerobic metabolism in Vibrio cholerae is required for population expansion during infection
    Article Snippet: .. Complementary cDNA was generated from RNA using Superscript III Reverse Transcriptase (Thermo Scientific). .. RT-qPCR reactions were carried out using SYBR Green Master Mix (Applied Biosystems) with 5ng cDNA.

    Cell Culture:

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Quantitative RT-PCR:

    Article Title: Fusarium oxysporum Triggers Tissue-Specific Transcriptional Reprogramming in Arabidopsis thaliana
    Article Snippet: .. F . oxysporum RT-qPCR First-strand cDNA was synthesized from 1μg RNA using SuperScript RNA H- Reverse Transcriptase (Invitrogen) and oligo (dT) primer, according to the manufacturer's instructions. .. RT-qPCR products were amplified in 10μl reactions containing 2μl cDNA, 2x SYBR® Green PCR Master Mix (Applied Biosystems) and 0.3 pmol primers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. RT-PCR Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

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  • 99
    Thermo Fisher superscript iii first strand synthesis system
    DRAIC knockout increases tumorigenic property of prostate cancer cells through NF-κB pathway. (A) A schematic illustration of DRAIC knockout strategy using CRISPR/Cas9 in LNCaP cells is shown. The sgRNAs were designed from DRAIC exon 2 and exon 4 as indicated by the arrowhead to knockout Exon 2–4. (B) <t>Three</t> representative single clones with KO of DRAIC identified by truncated PCR product from DRAIC genomic DNA. M: DNA molecular weight marker. (C) RT-qPCR of DRAIC <t>RNA</t> from WT and KO clones. Mean±s.d, n=4, **p
    Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii first strand synthesis system/product/Thermo Fisher
    Average 99 stars, based on 4953 article reviews
    Price from $9.99 to $1999.99
    superscript iii first strand synthesis system - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher total rna
    <t>RNA-seq</t> after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c – f Volcano plots of fold change vs p -value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c , MAB-MiPs untreated vs MAB-MiPs+PMC d , fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e , fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f . Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from <t>ChIP-qPCR</t> experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA ( n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total rna/product/Thermo Fisher
    Average 99 stars, based on 277 article reviews
    Price from $9.99 to $1999.99
    total rna - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript iii kit
    Detection of LASV clade II and IV. a Schematic of LASV SHERLOCK assays targeting the two most common clades of LASV: clades II (LASV-II assay) and IV (LASV-IV assay). For the LASV-II assay, <t>three</t> crRNAs were designed and tested. Two crRNAs are multiplexed to encompass the clade’s genetic diversity (IIA/IIB or IIA/IIC). Each crRNA was tested using three technical replicates. b – d Heat maps are measured in fluorescence (a.u.). b Detection of LASV RNA from suspected LF clinical samples using crRNAs IIA, IIB, IIC, or a combination of crRNAs. c Test of cross-reactivity between different viral species using MARV, EBOV, and LASV viral seedstock <t>cDNA.</t> The LASV-II and LASV-IV assays do not cross-react with MARV or EBOV seed stocks. d Test of cross-reactivity between LASV clade-specific assays using clinical samples from recent outbreaks in Nigeria and Sierra Leone. The LASV-II and LASV-IV assays provide clade-specific detection. e SHERLOCK testing using the LASV-II assay of RNA extracted from seven confirmed LASV-positive and three confirmed LASV-negative samples collected from suspected LF patients in Nigeria during the 2018 outbreak. Error bar indicates 95% confidence interval. f Results from e were compared head-to-head to those from the gold standard Nikisins RT-qPCR assay, next-generation sequencing (genome assembled), and lateral flow detection. g SHERLOCK testing using the LASV-IV assay of RNA extracted from seven confirmed LASV-positive and three confirmed LASV-negative samples collected from suspected LF patients in Sierra Leone. Error bar indicates 95% confidence interval. h Results from g were compared head-to-head to those from the gold standard Nikisins RT-qPCR assay, a second Broad RT-qPCR, NGS, and lateral flow detection. Source dare are in the Source Data file.
    Superscript Iii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii kit/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    superscript iii kit - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    DRAIC knockout increases tumorigenic property of prostate cancer cells through NF-κB pathway. (A) A schematic illustration of DRAIC knockout strategy using CRISPR/Cas9 in LNCaP cells is shown. The sgRNAs were designed from DRAIC exon 2 and exon 4 as indicated by the arrowhead to knockout Exon 2–4. (B) Three representative single clones with KO of DRAIC identified by truncated PCR product from DRAIC genomic DNA. M: DNA molecular weight marker. (C) RT-qPCR of DRAIC RNA from WT and KO clones. Mean±s.d, n=4, **p

    Journal: Cancer research

    Article Title: Long noncoding RNA DRAIC inhibits prostate cancer progression by interacting with IKK to inhibit NF-κB activation

    doi: 10.1158/0008-5472.CAN-19-3460

    Figure Lengend Snippet: DRAIC knockout increases tumorigenic property of prostate cancer cells through NF-κB pathway. (A) A schematic illustration of DRAIC knockout strategy using CRISPR/Cas9 in LNCaP cells is shown. The sgRNAs were designed from DRAIC exon 2 and exon 4 as indicated by the arrowhead to knockout Exon 2–4. (B) Three representative single clones with KO of DRAIC identified by truncated PCR product from DRAIC genomic DNA. M: DNA molecular weight marker. (C) RT-qPCR of DRAIC RNA from WT and KO clones. Mean±s.d, n=4, **p

    Article Snippet: 1 μg of total RNA was reverse transcribed using SuperScript III First-Strand cDNA synthesis kit (Thermo Fisher Scientific, catalog no. 18080051).

    Techniques: Knock-Out, CRISPR, Clone Assay, Polymerase Chain Reaction, Molecular Weight, Marker, Quantitative RT-PCR

    RNA-seq after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c – f Volcano plots of fold change vs p -value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c , MAB-MiPs untreated vs MAB-MiPs+PMC d , fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e , fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f . Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from ChIP-qPCR experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA ( n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)

    Journal: Nature Communications

    Article Title: MicroRNAs promote skeletal muscle differentiation of mesodermal iPSC-derived progenitors

    doi: 10.1038/s41467-017-01359-w

    Figure Lengend Snippet: RNA-seq after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c – f Volcano plots of fold change vs p -value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c , MAB-MiPs untreated vs MAB-MiPs+PMC d , fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e , fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f . Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from ChIP-qPCR experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA ( n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)

    Article Snippet: Gene qPCR was performed on 1:5 diluted cDNA obtained from 1 μg total RNA (SybrGreen mix, SSIII cDNA production kit and RNA extraction kit from Thermo Fisher Scientific), using Viia7 384-plate reader (Thermo Fisher Scientific; final primer concentration, 100 nM; final volume, 10 μl; PGK , internal reference; thermal profile, 95 °C 15 s, 60 °C 60 s, 40×). miRNA qPCR was performed on 1:15 diluted cDNA obtained from 20 ng total miRNA preparation (miRNA isolation kit, Thermo Fisher Scientific).

    Techniques: RNA Sequencing Assay, Quantitation Assay, Methylation Sequencing, Methylation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Marker, Derivative Assay

    Detection of LASV clade II and IV. a Schematic of LASV SHERLOCK assays targeting the two most common clades of LASV: clades II (LASV-II assay) and IV (LASV-IV assay). For the LASV-II assay, three crRNAs were designed and tested. Two crRNAs are multiplexed to encompass the clade’s genetic diversity (IIA/IIB or IIA/IIC). Each crRNA was tested using three technical replicates. b – d Heat maps are measured in fluorescence (a.u.). b Detection of LASV RNA from suspected LF clinical samples using crRNAs IIA, IIB, IIC, or a combination of crRNAs. c Test of cross-reactivity between different viral species using MARV, EBOV, and LASV viral seedstock cDNA. The LASV-II and LASV-IV assays do not cross-react with MARV or EBOV seed stocks. d Test of cross-reactivity between LASV clade-specific assays using clinical samples from recent outbreaks in Nigeria and Sierra Leone. The LASV-II and LASV-IV assays provide clade-specific detection. e SHERLOCK testing using the LASV-II assay of RNA extracted from seven confirmed LASV-positive and three confirmed LASV-negative samples collected from suspected LF patients in Nigeria during the 2018 outbreak. Error bar indicates 95% confidence interval. f Results from e were compared head-to-head to those from the gold standard Nikisins RT-qPCR assay, next-generation sequencing (genome assembled), and lateral flow detection. g SHERLOCK testing using the LASV-IV assay of RNA extracted from seven confirmed LASV-positive and three confirmed LASV-negative samples collected from suspected LF patients in Sierra Leone. Error bar indicates 95% confidence interval. h Results from g were compared head-to-head to those from the gold standard Nikisins RT-qPCR assay, a second Broad RT-qPCR, NGS, and lateral flow detection. Source dare are in the Source Data file.

    Journal: Nature Communications

    Article Title: Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

    doi: 10.1038/s41467-020-17994-9

    Figure Lengend Snippet: Detection of LASV clade II and IV. a Schematic of LASV SHERLOCK assays targeting the two most common clades of LASV: clades II (LASV-II assay) and IV (LASV-IV assay). For the LASV-II assay, three crRNAs were designed and tested. Two crRNAs are multiplexed to encompass the clade’s genetic diversity (IIA/IIB or IIA/IIC). Each crRNA was tested using three technical replicates. b – d Heat maps are measured in fluorescence (a.u.). b Detection of LASV RNA from suspected LF clinical samples using crRNAs IIA, IIB, IIC, or a combination of crRNAs. c Test of cross-reactivity between different viral species using MARV, EBOV, and LASV viral seedstock cDNA. The LASV-II and LASV-IV assays do not cross-react with MARV or EBOV seed stocks. d Test of cross-reactivity between LASV clade-specific assays using clinical samples from recent outbreaks in Nigeria and Sierra Leone. The LASV-II and LASV-IV assays provide clade-specific detection. e SHERLOCK testing using the LASV-II assay of RNA extracted from seven confirmed LASV-positive and three confirmed LASV-negative samples collected from suspected LF patients in Nigeria during the 2018 outbreak. Error bar indicates 95% confidence interval. f Results from e were compared head-to-head to those from the gold standard Nikisins RT-qPCR assay, next-generation sequencing (genome assembled), and lateral flow detection. g SHERLOCK testing using the LASV-IV assay of RNA extracted from seven confirmed LASV-positive and three confirmed LASV-negative samples collected from suspected LF patients in Sierra Leone. Error bar indicates 95% confidence interval. h Results from g were compared head-to-head to those from the gold standard Nikisins RT-qPCR assay, a second Broad RT-qPCR, NGS, and lateral flow detection. Source dare are in the Source Data file.

    Article Snippet: AMPure RNA clean beads (Beckman Coulter Genomics) were used to clean and concentrate samples. cDNA synthesis was performed using the Superscript III kit (Thermo Fischer) plus dNTPs, random primers, and SUPERASE-IN for first-strand synthesis.

    Techniques: Ii Assay, Fluorescence, Quantitative RT-PCR, Next-Generation Sequencing

    Detection of EBOV. a Schematic of the SHERLOCK EBOV assay. b , c Detection of a serial dilution of EBOV synthetic DNA using ( b ) mean fluorescence of three technical replicates and ( c ) lateral flow readouts. Error bars indicate ±1 SD for three technical replicates. d Test of cross-reactivity using MARV, EBOV, and LASV viral seedstock cDNA. Heat map is measured in Fluorescence (a.u.). e SHERLOCK testing of cDNA extracted from 12 confirmed EBOV-positive and 4 confirmed EBOV-negative samples collected from suspected EVD patients during the 2014 outbreak in Sierra Leone. Error bars indicate 95% confidence interval. f Four of the samples from e were also tested by collaborators using lateral flow detection. g , h Detection of serial dilution of synthetic RNA from Ituri, DRC and Makona, Sierra Leone using ( g ) fluorescence where error bars indicate ±1 SD for three technical replicates and ( h ) lateral flow readouts carried out at USAMRIID. Source data are in the Source Data file.

    Journal: Nature Communications

    Article Title: Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

    doi: 10.1038/s41467-020-17994-9

    Figure Lengend Snippet: Detection of EBOV. a Schematic of the SHERLOCK EBOV assay. b , c Detection of a serial dilution of EBOV synthetic DNA using ( b ) mean fluorescence of three technical replicates and ( c ) lateral flow readouts. Error bars indicate ±1 SD for three technical replicates. d Test of cross-reactivity using MARV, EBOV, and LASV viral seedstock cDNA. Heat map is measured in Fluorescence (a.u.). e SHERLOCK testing of cDNA extracted from 12 confirmed EBOV-positive and 4 confirmed EBOV-negative samples collected from suspected EVD patients during the 2014 outbreak in Sierra Leone. Error bars indicate 95% confidence interval. f Four of the samples from e were also tested by collaborators using lateral flow detection. g , h Detection of serial dilution of synthetic RNA from Ituri, DRC and Makona, Sierra Leone using ( g ) fluorescence where error bars indicate ±1 SD for three technical replicates and ( h ) lateral flow readouts carried out at USAMRIID. Source data are in the Source Data file.

    Article Snippet: AMPure RNA clean beads (Beckman Coulter Genomics) were used to clean and concentrate samples. cDNA synthesis was performed using the Superscript III kit (Thermo Fischer) plus dNTPs, random primers, and SUPERASE-IN for first-strand synthesis.

    Techniques: Serial Dilution, Fluorescence