superfect lipid transfection reagent  (Qiagen)

 
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    Name:
    SuperFect Transfection Reagent
    Description:
    For transfection of a broad range of eukaryotic cell lines with DNA Kit contents Qiagen SuperFect Transfection Reagent 1 2mL For 40 Transfections in 160mm Dishes or 640 Transfections in 12 well Plates Eukaryotic Cell Type Ideal for Transient and Stable Transfection of a Broad Range of Cell Lines Including 293 B16 BHK 21 COS 1 and CHO SuperFect Reagent is Suited for Studies on Gene Expression and Function Drug Discovery Development Studies Activated dendrimer Technology Excellent Reproducibility and Low Cytotoxicity Benefits Suitable for a broad range of cell lines Transfection can be performed in the presence of serum Activated dendrimers ensure reproducibility
    Catalog Number:
    301305
    Price:
    312
    Category:
    SuperFect Transfection Reagent
    Buy from Supplier


    Structured Review

    Qiagen superfect lipid transfection reagent
    SuperFect Transfection Reagent
    For transfection of a broad range of eukaryotic cell lines with DNA Kit contents Qiagen SuperFect Transfection Reagent 1 2mL For 40 Transfections in 160mm Dishes or 640 Transfections in 12 well Plates Eukaryotic Cell Type Ideal for Transient and Stable Transfection of a Broad Range of Cell Lines Including 293 B16 BHK 21 COS 1 and CHO SuperFect Reagent is Suited for Studies on Gene Expression and Function Drug Discovery Development Studies Activated dendrimer Technology Excellent Reproducibility and Low Cytotoxicity Benefits Suitable for a broad range of cell lines Transfection can be performed in the presence of serum Activated dendrimers ensure reproducibility
    https://www.bioz.com/result/superfect lipid transfection reagent/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superfect lipid transfection reagent - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Gadolinium conjugated TiO2-DNA oligonucleotide nanoconjugates show prolonged intracellular retention period and T1-weighted contrast enhancement in Magnetic Resonance images
    Article Snippet: .. These two different types of cells were either transfected by electroporation or chemically (SuperFect reagent) with complete nanoconjugate or its components. shows a set of T1 -weighted images acquired at an inversion time of 1000 ms. Signal enhancement was only observed in those samples that contained cells treated with TiO2 -DNA-Gd while cell samples treated with TiO2 or “free” Gd CA showed no T1 -weighted enhancement at this time. ..

    Transfection:

    Article Title: Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells
    Article Snippet: .. ApH4ON or a mock cis element corresponding to OctON (octamer transcription factor binding cis element oligonucleotide) were transfected using SuperFect™ reagent following the manufacturer's instructions (Qiagen). ..

    Article Title: CREB Activation Induces Adipogenesis in 3T3-L1 Cells
    Article Snippet: .. Plates of 3T3-L1 fibroblasts and adipocytes were grown to 70 to 80% confluency and transfected with the indicated plasmids with Superfect Reagent (Qiagen, Valencia, Calif.) according to the manufacturer's recommendations. .. Cells stably transfected with the plasmid pVgRXR were selected in conventional medium containing 500 μg of Zeocin per ml, and cells stably transfected with pIND-VP16-CREB, pIND-KCREB (or pIND-VP16-KCREB and pIND-LacZ) plasmids were selected in medium containing 500 μg of Geneticin per ml.

    Article Title: Protein Kinase A Regulates Cholinergic Gene Expression in PC12 Cells: REST4 Silences the Silencing Activity of Neuron-Restrictive Silencer Factor/REST
    Article Snippet: .. SuperFect transfection reagent, plasmid kits, and RNeasy MINI kits (Total RNA system) were obtained from Qiagen Inc. (Valencia, Calif.). .. The GalactoLight system was purchased from Tropix Inc. (Bedford, Mass.).

    Article Title: Primary activation of interferon A and interferon B gene transcription by interferon regulatory factor 3
    Article Snippet: .. For the transient transfection assay, subconfluent cells (5 × 105 /60-mm dish) were transfected with 1–2 μg of the reporter plasmids and indicated amounts of IRF expression plasmids using Superfect transfection reagent (Qiagen). .. The total amount of DNA used in each transfection was kept constant, and the β-galactosidase expressing plasmid (0.1 μg) was included as internal standard.

    Expressing:

    Article Title: Primary activation of interferon A and interferon B gene transcription by interferon regulatory factor 3
    Article Snippet: .. For the transient transfection assay, subconfluent cells (5 × 105 /60-mm dish) were transfected with 1–2 μg of the reporter plasmids and indicated amounts of IRF expression plasmids using Superfect transfection reagent (Qiagen). .. The total amount of DNA used in each transfection was kept constant, and the β-galactosidase expressing plasmid (0.1 μg) was included as internal standard.

    Incubation:

    Article Title: NF-?B Controls Cell Growth and Differentiation through Transcriptional Regulation of Cyclin D1
    Article Snippet: .. The following day, a total of 2.5 μg of plasmid DNA was incubated with Superfect as recommended by the manufacturer (Qiagen). ..

    Mass Spectrometry:

    Article Title: Gadolinium conjugated TiO2-DNA oligonucleotide nanoconjugates show prolonged intracellular retention period and T1-weighted contrast enhancement in Magnetic Resonance images
    Article Snippet: .. These two different types of cells were either transfected by electroporation or chemically (SuperFect reagent) with complete nanoconjugate or its components. shows a set of T1 -weighted images acquired at an inversion time of 1000 ms. Signal enhancement was only observed in those samples that contained cells treated with TiO2 -DNA-Gd while cell samples treated with TiO2 or “free” Gd CA showed no T1 -weighted enhancement at this time. ..

    Binding Assay:

    Article Title: Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells
    Article Snippet: .. ApH4ON or a mock cis element corresponding to OctON (octamer transcription factor binding cis element oligonucleotide) were transfected using SuperFect™ reagent following the manufacturer's instructions (Qiagen). ..

    Transient Transfection Assay:

    Article Title: Primary activation of interferon A and interferon B gene transcription by interferon regulatory factor 3
    Article Snippet: .. For the transient transfection assay, subconfluent cells (5 × 105 /60-mm dish) were transfected with 1–2 μg of the reporter plasmids and indicated amounts of IRF expression plasmids using Superfect transfection reagent (Qiagen). .. The total amount of DNA used in each transfection was kept constant, and the β-galactosidase expressing plasmid (0.1 μg) was included as internal standard.

    Plasmid Preparation:

    Article Title: Protein Kinase A Regulates Cholinergic Gene Expression in PC12 Cells: REST4 Silences the Silencing Activity of Neuron-Restrictive Silencer Factor/REST
    Article Snippet: .. SuperFect transfection reagent, plasmid kits, and RNeasy MINI kits (Total RNA system) were obtained from Qiagen Inc. (Valencia, Calif.). .. The GalactoLight system was purchased from Tropix Inc. (Bedford, Mass.).

    Article Title: NF-?B Controls Cell Growth and Differentiation through Transcriptional Regulation of Cyclin D1
    Article Snippet: .. The following day, a total of 2.5 μg of plasmid DNA was incubated with Superfect as recommended by the manufacturer (Qiagen). ..

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  • Logo
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  • Bioz Stars
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  • 99
    Qiagen superfect
    Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), <t>SuperFect</t> (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p
    Superfect, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfect/product/Qiagen
    Average 99 stars, based on 1372 article reviews
    Price from $9.99 to $1999.99
    superfect - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: Transfection, Quantitative RT-PCR, Expressing, MTT Assay