superfect lipid transfection reagent  (Qiagen)

 
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    Name:
    SuperFect Transfection Reagent
    Description:
    For transfection of a broad range of eukaryotic cell lines with DNA Kit contents Qiagen SuperFect Transfection Reagent 1 2mL For 40 Transfections in 160mm Dishes or 640 Transfections in 12 well Plates Eukaryotic Cell Type Ideal for Transient and Stable Transfection of a Broad Range of Cell Lines Including 293 B16 BHK 21 COS 1 and CHO SuperFect Reagent is Suited for Studies on Gene Expression and Function Drug Discovery Development Studies Activated dendrimer Technology Excellent Reproducibility and Low Cytotoxicity Benefits Suitable for a broad range of cell lines Transfection can be performed in the presence of serum Activated dendrimers ensure reproducibility
    Catalog Number:
    301305
    Price:
    312
    Category:
    SuperFect Transfection Reagent
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    Structured Review

    Qiagen superfect lipid transfection reagent
    SuperFect Transfection Reagent
    For transfection of a broad range of eukaryotic cell lines with DNA Kit contents Qiagen SuperFect Transfection Reagent 1 2mL For 40 Transfections in 160mm Dishes or 640 Transfections in 12 well Plates Eukaryotic Cell Type Ideal for Transient and Stable Transfection of a Broad Range of Cell Lines Including 293 B16 BHK 21 COS 1 and CHO SuperFect Reagent is Suited for Studies on Gene Expression and Function Drug Discovery Development Studies Activated dendrimer Technology Excellent Reproducibility and Low Cytotoxicity Benefits Suitable for a broad range of cell lines Transfection can be performed in the presence of serum Activated dendrimers ensure reproducibility
    https://www.bioz.com/result/superfect lipid transfection reagent/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superfect lipid transfection reagent - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: NF-?B Controls Cell Growth and Differentiation through Transcriptional Regulation of Cyclin D1
    Article Snippet: .. The following day, a total of 2.5 μg of plasmid DNA was incubated with Superfect as recommended by the manufacturer (Qiagen). ..

    Article Title: Abnormal Mammary Gland Development and Growth Retardation in Female Mice and MCF7 Breast Cancer Cells Lacking Androgen Receptor
    Article Snippet: .. For generation of AR −/− MCF7 cells, parental MCF7 cells were transfected with the AatII-linearized AR targeting vector using SuperFect (QIAGEN) and selected with 400 μg/ml neomycin. .. The genotypes of surviving clones were screened by Southern blot analyses.

    Transfection:

    Article Title: Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells
    Article Snippet: .. ApH4ON or a mock cis element corresponding to OctON (octamer transcription factor binding cis element oligonucleotide) were transfected using SuperFect™ reagent following the manufacturer's instructions (Qiagen). ..

    Article Title: Expression of the Mouse Pre-T Cell Receptor ? Gene Is Controlled by an Upstream Region Containing a Transcriptional Enhancer
    Article Snippet: .. Cell lines LR1, BW5147, MEL, and NIH3T3 were transfected using Fugene 6 reagent (Roche Molecular Biochemicals); cells from cell lines LR2 and 642 were transfected using Superfect reagent (Qiagen). ..

    Article Title: Atg3-Mediated Lipidation of Atg8 Is Involved in Encystation of Acanthamoeba
    Article Snippet: .. Transient transfection was performed by Superfect transfection reagent (Qiagen, Valencia, California, USA) as previously described [ ]. .. Atg3 gene silencing by siRNA Transfection of siRNA was performed for the confirmation of gene silencing of AcAtg3.

    Article Title: Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells
    Article Snippet: .. The transfection reagent, SuperFect™, was purchased from Qiagen (Chatsworth, CA, U.S.A.). .. Primary renal cell lines LLC-PK1, generated from porcine proximal tubules were purchased from A.T.C.C. (Manassas, VA, U.S.A.).

    Article Title: Abnormal Mammary Gland Development and Growth Retardation in Female Mice and MCF7 Breast Cancer Cells Lacking Androgen Receptor
    Article Snippet: .. For generation of AR −/− MCF7 cells, parental MCF7 cells were transfected with the AatII-linearized AR targeting vector using SuperFect (QIAGEN) and selected with 400 μg/ml neomycin. .. The genotypes of surviving clones were screened by Southern blot analyses.

    Article Title: Increase of androgen-induced cell death and androgen receptor transactivation by BRCA1 in prostate cancer cells
    Article Snippet: .. LNCaP T47D and MCF-7 cells were transfected by using SuperFect (Qiagen, Chatsworth, CA). .. GST–BRCA1 fusion proteins and GST control protein were purified as described by the manufacturer (Amersham Pharmacia).

    other:

    Article Title: Triazine dendrimers as non-viral gene delivery systems: Effects of molecular structure on biological activity
    Article Snippet: Poly(ethylene imine) (Polymin™ , 25 kDa) was a gift from BASF (Ludwigshafen, Germany), PAMAM (ethylenediamine core, amino surface) 2nd and 3rd generation was bought from Sigma-Aldrich Laborchemikalien GmbH, Seelze, Germany and SuperFect™ from Qiagen, Hilden, Germany.

    Binding Assay:

    Article Title: Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells
    Article Snippet: .. ApH4ON or a mock cis element corresponding to OctON (octamer transcription factor binding cis element oligonucleotide) were transfected using SuperFect™ reagent following the manufacturer's instructions (Qiagen). ..

    Incubation:

    Article Title: NF-?B Controls Cell Growth and Differentiation through Transcriptional Regulation of Cyclin D1
    Article Snippet: .. The following day, a total of 2.5 μg of plasmid DNA was incubated with Superfect as recommended by the manufacturer (Qiagen). ..

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  • 99
    Qiagen superfect
    Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), <t>SuperFect</t> (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p
    Superfect, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfect/product/Qiagen
    Average 99 stars, based on 1370 article reviews
    Price from $9.99 to $1999.99
    superfect - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: Transfection, Quantitative RT-PCR, Expressing, MTT Assay