Structured Review

Promega superase
Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or <t>SUPERase·</t> In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.
Superase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superase/product/Promega
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99
superase - by Bioz Stars, 2020-09
99/100 stars

Images

1) Product Images from "Characterization of the ribonuclease activity on the skin surface"

Article Title: Characterization of the ribonuclease activity on the skin surface

Journal: Genetic Vaccines and Therapy

doi: 10.1186/1479-0556-4-4

Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or SUPERase· In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.
Figure Legend Snippet: Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or SUPERase· In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.

Techniques Used: Activity Assay, Concentration Assay, Incubation

Related Articles

RNA Extraction:

Article Title: Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses
Article Snippet: .. exRNA stability To test whether synthetic miRNA-like oligoribonucleotide sequences were intrinsically stable in BALF, 22 nucleotide RNA calibrators (calibrators 1-4 ( )) were spiked into BALF and incubated for 5min at 37°C with or without RNAsin ribonuclease inhibitor (Promega) before RNA extraction. .. To test whether endogenous ex-miRNAs were stable to exogenous RNases, RNase A (Thermo Scientific) was added to BALF or total RNA previously isolated from BALF at 0.04-40ug/ml and incubated for 37°C for 15min before RNA extraction.

Article Title: Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses
Article Snippet: .. To test whether synthetic miRNA-like oligoribonucleotide sequences were intrinsically stable in BALF, 22 nucleotide RNA calibrators (calibrators 1-4 ( )) were spiked into BALF and incubated for 5min at 37°C with or without RNAsin ribonuclease inhibitor (Promega) before RNA extraction. .. To test whether endogenous ex-miRNAs were stable to exogenous RNases, RNase A (Thermo Scientific) was added to BALF or total RNA previously isolated from BALF at 0.04-40ug/ml and incubated for 37°C for 15min before RNA extraction.

Amplification:

Article Title: Chicken Cells Sense Influenza A Virus Infection through MDA5 and CARDIF Signaling Involving LGP2
Article Snippet: .. RNA was extracted using TRIzol (Invitrogen), DNase I (Ambion), and RNase inhibitor (RNasin Plus; Promega) or with the Nucleospin RNA II kit (Macherey-Nagel) and amplified with the SuperScript III platinum one-step quantitative RT-PCR system (Invitrogen) using oligonucleotide primers and probes ( ) purchased from Microsynth. ..

In Vitro:

Article Title: Structural insight into the human mitochondrial tRNA purine N1-methyltransferase and ribonuclease P complexes
Article Snippet: .. RNase P activity assay RNase P cleavage was performed by mixing 300 nm MRPP1–MRPP2, 150 nm MRPP3, 10 units of RNase inhibitors (RNasin from Promega), and 400 nm in vitro transcribed pre-(mt) tRNAIle in a buffer of 30 mm Tris-HCl, pH 8, 40 mm NaCl, 4.5 mm MgCl2 , and 2 mm DTT to a total reaction volume of 8.25 μl. .. The reaction was performed at room temperature and stopped at set times by transferring 1 μl of the reaction mixture into 5 μl of 500 mm EDTA and heating to 95 °C.

Quantitative RT-PCR:

Article Title: Chicken Cells Sense Influenza A Virus Infection through MDA5 and CARDIF Signaling Involving LGP2
Article Snippet: .. RNA was extracted using TRIzol (Invitrogen), DNase I (Ambion), and RNase inhibitor (RNasin Plus; Promega) or with the Nucleospin RNA II kit (Macherey-Nagel) and amplified with the SuperScript III platinum one-step quantitative RT-PCR system (Invitrogen) using oligonucleotide primers and probes ( ) purchased from Microsynth. ..

Concentration Assay:

Article Title: Evaluation of human pancreatic RNase as effector molecule in a therapeutic antibody platform
Article Snippet: .. In addition, RNase activity of MN-IgG-RNase was also tested in the presence of a concentration series of up to 50 fold molar excess of human placental RNase inhibitor (RNasin Plus, Promega). .. Bovine RNase (Applichem) was used as positive control for RI mediated activity inhibition.

Incubation:

Article Title: ALS mutant FUS disrupts nuclear localization and sequesters wild-type FUS within cytoplasmic stress granules
Article Snippet: .. 5 µl of an RNase A/T1 mix (Fermentas, Yorkshire, UK) or RNasin Plus RNase inhibitor (Promega, Southampton, UK) was added to the tubes and incubated at 37°C for 5 min, then placed back on ice. .. Mouse anti-Myc (Cell Signalling Technology, 1:100), rabbit anti-HA (Cell Signalling Technology, 1:100) or mouse anti-FUS (Santa Cruz Biotechnology, Inc., 1:100) was added to the lysates as appropriate followed by the addition of 20 µl of protein A beads (Invitrogen Dynabeads).

Article Title: Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses
Article Snippet: .. exRNA stability To test whether synthetic miRNA-like oligoribonucleotide sequences were intrinsically stable in BALF, 22 nucleotide RNA calibrators (calibrators 1-4 ( )) were spiked into BALF and incubated for 5min at 37°C with or without RNAsin ribonuclease inhibitor (Promega) before RNA extraction. .. To test whether endogenous ex-miRNAs were stable to exogenous RNases, RNase A (Thermo Scientific) was added to BALF or total RNA previously isolated from BALF at 0.04-40ug/ml and incubated for 37°C for 15min before RNA extraction.

Article Title: Telomerase in kinetoplastid parasitic protozoa
Article Snippet: .. Activity in the extracts was tested for RNase A sensitivity by incubation with 100 ng of RNase A (Sigma) for 5 min at 37°C before or after the telomerase reaction step and with or without 1 unit of RNase inhibitor RNasin (Promega) before addition of RNase A. ..

Article Title: Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses
Article Snippet: .. To test whether synthetic miRNA-like oligoribonucleotide sequences were intrinsically stable in BALF, 22 nucleotide RNA calibrators (calibrators 1-4 ( )) were spiked into BALF and incubated for 5min at 37°C with or without RNAsin ribonuclease inhibitor (Promega) before RNA extraction. .. To test whether endogenous ex-miRNAs were stable to exogenous RNases, RNase A (Thermo Scientific) was added to BALF or total RNA previously isolated from BALF at 0.04-40ug/ml and incubated for 37°C for 15min before RNA extraction.

other:

Article Title: DNA Aptamers to Human Immunodeficiency Virus Reverse Transcriptase Selected by a Primer-Free SELEX Method: Characterization and Comparison with Other Aptamers
Article Snippet: RNase inhibitor (RNasin) was from Promega.

Activity Assay:

Article Title: Evaluation of human pancreatic RNase as effector molecule in a therapeutic antibody platform
Article Snippet: .. In addition, RNase activity of MN-IgG-RNase was also tested in the presence of a concentration series of up to 50 fold molar excess of human placental RNase inhibitor (RNasin Plus, Promega). .. Bovine RNase (Applichem) was used as positive control for RI mediated activity inhibition.

Article Title: Telomerase in kinetoplastid parasitic protozoa
Article Snippet: .. Activity in the extracts was tested for RNase A sensitivity by incubation with 100 ng of RNase A (Sigma) for 5 min at 37°C before or after the telomerase reaction step and with or without 1 unit of RNase inhibitor RNasin (Promega) before addition of RNase A. ..

Article Title: Structural insight into the human mitochondrial tRNA purine N1-methyltransferase and ribonuclease P complexes
Article Snippet: .. RNase P activity assay RNase P cleavage was performed by mixing 300 nm MRPP1–MRPP2, 150 nm MRPP3, 10 units of RNase inhibitors (RNasin from Promega), and 400 nm in vitro transcribed pre-(mt) tRNAIle in a buffer of 30 mm Tris-HCl, pH 8, 40 mm NaCl, 4.5 mm MgCl2 , and 2 mm DTT to a total reaction volume of 8.25 μl. .. The reaction was performed at room temperature and stopped at set times by transferring 1 μl of the reaction mixture into 5 μl of 500 mm EDTA and heating to 95 °C.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Promega superase
    Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or <t>SUPERase·</t> In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.
    Superase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase/product/Promega
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    superase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Promega superase• in rnase inhibitor
    Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or <t>SUPERase·</t> In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.
    Superase• In Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase• in rnase inhibitor/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superase• in rnase inhibitor - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or SUPERase· In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.

    Journal: Genetic Vaccines and Therapy

    Article Title: Characterization of the ribonuclease activity on the skin surface

    doi: 10.1186/1479-0556-4-4

    Figure Lengend Snippet: Trans-protection of mRNA against skin surface ribonuclease activity . Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin ® (R-in, final concentration 1 U/μl) or SUPERase· In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.

    Article Snippet: Ribonuclease inhibitors The ribonuclease inhibitors RNasin® and SUPERase· In™ were purchased from Promega (Mannheim, Germany) and Ambion (Huntingdon, UK).

    Techniques: Activity Assay, Concentration Assay, Incubation