substrate dimethyl lys 4 h3 21 solution  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical substrate dimethyl lys 4 h3 21 solution
    Spectroscopic analysis of cis or trans <t>-3-chloroallyl-Lys-4</t> <t>H3-21</t> ( 3 and 4 ) treated GST-LSD1. UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. A) LSD1 treated with
    Substrate Dimethyl Lys 4 H3 21 Solution, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/substrate dimethyl lys 4 h3 21 solution/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    substrate dimethyl lys 4 h3 21 solution - by Bioz Stars, 2020-07
    85/100 stars

    Images

    1) Product Images from "Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors"

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    Journal: Journal of the American Chemical Society

    doi: 10.1021/ja909996p

    Spectroscopic analysis of cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ) treated GST-LSD1. UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. A) LSD1 treated with
    Figure Legend Snippet: Spectroscopic analysis of cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ) treated GST-LSD1. UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. A) LSD1 treated with

    Techniques Used:

    Spectroscopic and MALDI-TOF analysis of hydrazino-Lys-4 H3-21 ( 18 ) treated GST-LSD1. A) UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. LSD1 treated with 18 (red)
    Figure Legend Snippet: Spectroscopic and MALDI-TOF analysis of hydrazino-Lys-4 H3-21 ( 18 ) treated GST-LSD1. A) UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. LSD1 treated with 18 (red)

    Techniques Used:

    Synthesis of peptide inhibitors cis -3-chloroallyl-Lys-4 H3-21 ( 3 ), trans -3-chloroallyl-Lys-4 H3-21 ( 4 ) and hydrazino-Lys-4 H3-21 ( 18 ). Incorporation of Fmoc-hydroxynorleucine in the four position of H3-21 yields the resin bound peptide. Mesylation of
    Figure Legend Snippet: Synthesis of peptide inhibitors cis -3-chloroallyl-Lys-4 H3-21 ( 3 ), trans -3-chloroallyl-Lys-4 H3-21 ( 4 ) and hydrazino-Lys-4 H3-21 ( 18 ). Incorporation of Fmoc-hydroxynorleucine in the four position of H3-21 yields the resin bound peptide. Mesylation of

    Techniques Used:

    Time- and concentration-dependent inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ) and trans -3-chloroallyl-Lys-4 H3-21 ( 4 ). A and C) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of
    Figure Legend Snippet: Time- and concentration-dependent inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ) and trans -3-chloroallyl-Lys-4 H3-21 ( 4 ). A and C) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of

    Techniques Used: Concentration Assay

    Inhibition of GST-LSD1 by endo -cyclopropyl-Lys-4 H3-21 ( 6 ) and endo -dimethylcyclopropyl-Lys-4 H3-21 ( 7 ). A and C) Steady-state progress curves obtained for the inhibition of LSD1 in the presence of concentrations of 6 ranging from 0 to 49.6 µM
    Figure Legend Snippet: Inhibition of GST-LSD1 by endo -cyclopropyl-Lys-4 H3-21 ( 6 ) and endo -dimethylcyclopropyl-Lys-4 H3-21 ( 7 ). A and C) Steady-state progress curves obtained for the inhibition of LSD1 in the presence of concentrations of 6 ranging from 0 to 49.6 µM

    Techniques Used: Inhibition

    Proposed mechanism of inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). Pathway a , two electron oxidation of 18 by the flavin yields the diazene. A second oxidation to the primary diazonium species produces a powerful electrophilic site at the alpha
    Figure Legend Snippet: Proposed mechanism of inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). Pathway a , two electron oxidation of 18 by the flavin yields the diazene. A second oxidation to the primary diazonium species produces a powerful electrophilic site at the alpha

    Techniques Used:

    Time- and concentration-dependent inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). A) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of 18 ranging from 0.1 to 0.4 µM. B) Rate constants
    Figure Legend Snippet: Time- and concentration-dependent inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). A) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of 18 ranging from 0.1 to 0.4 µM. B) Rate constants

    Techniques Used: Concentration Assay

    Proposed mechanism of inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ). Flavin mediated two electron oxidation of 3 yields an α,β-unsaturated iminium species. Pathway a ) Nucleophilic attack by the N 5 of the reduced flavin
    Figure Legend Snippet: Proposed mechanism of inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ). Flavin mediated two electron oxidation of 3 yields an α,β-unsaturated iminium species. Pathway a ) Nucleophilic attack by the N 5 of the reduced flavin

    Techniques Used:

    MALDI-TOF assay demonstrating the inactivation of GST-LSD1 by phenelzine. A) LSD1 preincubated with phenelzine prior to exposure to dimethyl-Lys-4 H3-21 substrate fails to appreciably demethylate. B) LSD1 efficiently demethylates dimethyl-Lys-4 H3-21
    Figure Legend Snippet: MALDI-TOF assay demonstrating the inactivation of GST-LSD1 by phenelzine. A) LSD1 preincubated with phenelzine prior to exposure to dimethyl-Lys-4 H3-21 substrate fails to appreciably demethylate. B) LSD1 efficiently demethylates dimethyl-Lys-4 H3-21

    Techniques Used:

    MALDI-TOF analysis of GST-LSD1 incubated with cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ). A) Inactivator 3 is seen as a minor peak, while a new signal corresponding to the predicted mass of a 3 -FAD adduct is now apparent. Additionally, a signal
    Figure Legend Snippet: MALDI-TOF analysis of GST-LSD1 incubated with cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ). A) Inactivator 3 is seen as a minor peak, while a new signal corresponding to the predicted mass of a 3 -FAD adduct is now apparent. Additionally, a signal

    Techniques Used: Incubation

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    Worthington Biochemical substrate dimethyl lys 4 h3 21 solution
    Spectroscopic analysis of cis or trans <t>-3-chloroallyl-Lys-4</t> <t>H3-21</t> ( 3 and 4 ) treated GST-LSD1. UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. A) LSD1 treated with
    Substrate Dimethyl Lys 4 H3 21 Solution, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/substrate dimethyl lys 4 h3 21 solution/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    substrate dimethyl lys 4 h3 21 solution - by Bioz Stars, 2020-07
    85/100 stars
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    Spectroscopic analysis of cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ) treated GST-LSD1. UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. A) LSD1 treated with

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Spectroscopic analysis of cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ) treated GST-LSD1. UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. A) LSD1 treated with

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques:

    Spectroscopic and MALDI-TOF analysis of hydrazino-Lys-4 H3-21 ( 18 ) treated GST-LSD1. A) UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. LSD1 treated with 18 (red)

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Spectroscopic and MALDI-TOF analysis of hydrazino-Lys-4 H3-21 ( 18 ) treated GST-LSD1. A) UV/Vis spectra of native LSD1 (blue) shows the distinctive two maxima of the fully oxidized flavin and the one electron reduced semiquinone. LSD1 treated with 18 (red)

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques:

    Synthesis of peptide inhibitors cis -3-chloroallyl-Lys-4 H3-21 ( 3 ), trans -3-chloroallyl-Lys-4 H3-21 ( 4 ) and hydrazino-Lys-4 H3-21 ( 18 ). Incorporation of Fmoc-hydroxynorleucine in the four position of H3-21 yields the resin bound peptide. Mesylation of

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Synthesis of peptide inhibitors cis -3-chloroallyl-Lys-4 H3-21 ( 3 ), trans -3-chloroallyl-Lys-4 H3-21 ( 4 ) and hydrazino-Lys-4 H3-21 ( 18 ). Incorporation of Fmoc-hydroxynorleucine in the four position of H3-21 yields the resin bound peptide. Mesylation of

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques:

    Time- and concentration-dependent inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ) and trans -3-chloroallyl-Lys-4 H3-21 ( 4 ). A and C) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Time- and concentration-dependent inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ) and trans -3-chloroallyl-Lys-4 H3-21 ( 4 ). A and C) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques: Concentration Assay

    Inhibition of GST-LSD1 by endo -cyclopropyl-Lys-4 H3-21 ( 6 ) and endo -dimethylcyclopropyl-Lys-4 H3-21 ( 7 ). A and C) Steady-state progress curves obtained for the inhibition of LSD1 in the presence of concentrations of 6 ranging from 0 to 49.6 µM

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Inhibition of GST-LSD1 by endo -cyclopropyl-Lys-4 H3-21 ( 6 ) and endo -dimethylcyclopropyl-Lys-4 H3-21 ( 7 ). A and C) Steady-state progress curves obtained for the inhibition of LSD1 in the presence of concentrations of 6 ranging from 0 to 49.6 µM

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques: Inhibition

    Proposed mechanism of inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). Pathway a , two electron oxidation of 18 by the flavin yields the diazene. A second oxidation to the primary diazonium species produces a powerful electrophilic site at the alpha

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Proposed mechanism of inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). Pathway a , two electron oxidation of 18 by the flavin yields the diazene. A second oxidation to the primary diazonium species produces a powerful electrophilic site at the alpha

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques:

    Time- and concentration-dependent inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). A) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of 18 ranging from 0.1 to 0.4 µM. B) Rate constants

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Time- and concentration-dependent inactivation of GST-LSD1 by hydrazino-Lys-4 H3-21 ( 18 ). A) Steady-state progress curves obtained for the inactivation of LSD1 in the presence of concentrations of 18 ranging from 0.1 to 0.4 µM. B) Rate constants

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques: Concentration Assay

    Proposed mechanism of inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ). Flavin mediated two electron oxidation of 3 yields an α,β-unsaturated iminium species. Pathway a ) Nucleophilic attack by the N 5 of the reduced flavin

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: Proposed mechanism of inactivation of GST-LSD1 by cis -3-chloroallyl-Lys-4 H3-21 ( 3 ). Flavin mediated two electron oxidation of 3 yields an α,β-unsaturated iminium species. Pathway a ) Nucleophilic attack by the N 5 of the reduced flavin

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques:

    MALDI-TOF assay demonstrating the inactivation of GST-LSD1 by phenelzine. A) LSD1 preincubated with phenelzine prior to exposure to dimethyl-Lys-4 H3-21 substrate fails to appreciably demethylate. B) LSD1 efficiently demethylates dimethyl-Lys-4 H3-21

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: MALDI-TOF assay demonstrating the inactivation of GST-LSD1 by phenelzine. A) LSD1 preincubated with phenelzine prior to exposure to dimethyl-Lys-4 H3-21 substrate fails to appreciably demethylate. B) LSD1 efficiently demethylates dimethyl-Lys-4 H3-21

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques:

    MALDI-TOF analysis of GST-LSD1 incubated with cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ). A) Inactivator 3 is seen as a minor peak, while a new signal corresponding to the predicted mass of a 3 -FAD adduct is now apparent. Additionally, a signal

    Journal: Journal of the American Chemical Society

    Article Title: Comparative Analysis of Small Molecules and Histone Substrate Analogs as LSD1 Lysine Demethylase Inhibitors

    doi: 10.1021/ja909996p

    Figure Lengend Snippet: MALDI-TOF analysis of GST-LSD1 incubated with cis or trans -3-chloroallyl-Lys-4 H3-21 ( 3 and 4 ). A) Inactivator 3 is seen as a minor peak, while a new signal corresponding to the predicted mass of a 3 -FAD adduct is now apparent. Additionally, a signal

    Article Snippet: The 150 µL reactions were initiated by the addition of 50 µl of buffered substrate (dimethyl-Lys-4 H3-21) solution to reaction mixtures (100 µl) consisting of 50 mM HEPES buffer (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.76 µM horseradish peroxidase (Worthington Biochemical Corporation), and 185 nM GST-LSD1.

    Techniques: Incubation