subcloning efficiency dh5α competent cells  (Thermo Fisher)


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    Name:
    Subcloning Efficiency DH5α Competent Cells
    Description:
    Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of 1 x 106 cfu µg plasmid DNA Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting Features of Subcloning Efficiency DH5α Competent Cells • Designed for general everyday use• Contain genetic markers useful for general applicationsIdeal for routine subcloning proceduresSubcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries These economical cells yield 1 x 106 transformants µg control DNA per 50 µL reaction Flexible cloning capabilitiesSubcloning Efficiency DH5α Competent Cells contain the following genetic markers resulting in these benefits • lacZΔM15 for blue white color screening of colonies on plates containing X gal or Bluo gal• recA1 ensures increased insert stability and prevents unwanted recombination• endA1 improves the yield and quality of plasmid DNA prepared from minipreps• DH5α competent cells support replication of M13mp vectors but do not support plaque formationGenotype F Φ80lacZΔM15 Δ lacZYA argF U169 recA1 endA1 hsdR17 rk mk phoA supE44 thi 1 gyrA96 relA1 λ
    Catalog Number:
    18265017
    Price:
    None
    Applications:
    Chemically Competent Cells for Cloning|Cloning|Transformation
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher subcloning efficiency dh5α competent cells
    D1 anaerobic protein purity. Protein was expressed in <t>DH5α</t> from the pASG-IBA35 vector A. Steps of D1 anaerobic purification process. Numbers above the gel mark fractions: 1 – before induction, 2- after induction, 3 – lysate, 4- pellet after centrifugation at 40 000 rcf, 5- NiNTA flow through, 6 – elution (this D1 anaerobic protein batch was used for EXAFS experiments). The marked band with mass 30 kDa corresponds to D1 protein. B. The purity of D1 anaerobic protein batch used for TSA, crystallization and SEC HPLC analysis (lanes 1 and 2).
    Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of 1 x 106 cfu µg plasmid DNA Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting Features of Subcloning Efficiency DH5α Competent Cells • Designed for general everyday use• Contain genetic markers useful for general applicationsIdeal for routine subcloning proceduresSubcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries These economical cells yield 1 x 106 transformants µg control DNA per 50 µL reaction Flexible cloning capabilitiesSubcloning Efficiency DH5α Competent Cells contain the following genetic markers resulting in these benefits • lacZΔM15 for blue white color screening of colonies on plates containing X gal or Bluo gal• recA1 ensures increased insert stability and prevents unwanted recombination• endA1 improves the yield and quality of plasmid DNA prepared from minipreps• DH5α competent cells support replication of M13mp vectors but do not support plaque formationGenotype F Φ80lacZΔM15 Δ lacZYA argF U169 recA1 endA1 hsdR17 rk mk phoA supE44 thi 1 gyrA96 relA1 λ
    https://www.bioz.com/result/subcloning efficiency dh5α competent cells/product/Thermo Fisher
    Average 96 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    subcloning efficiency dh5α competent cells - by Bioz Stars, 2020-08
    96/100 stars

    Images

    1) Product Images from "Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterization"

    Article Title: Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterization

    Journal: Protein expression and purification

    doi: 10.1016/j.pep.2017.03.019

    D1 anaerobic protein purity. Protein was expressed in DH5α from the pASG-IBA35 vector A. Steps of D1 anaerobic purification process. Numbers above the gel mark fractions: 1 – before induction, 2- after induction, 3 – lysate, 4- pellet after centrifugation at 40 000 rcf, 5- NiNTA flow through, 6 – elution (this D1 anaerobic protein batch was used for EXAFS experiments). The marked band with mass 30 kDa corresponds to D1 protein. B. The purity of D1 anaerobic protein batch used for TSA, crystallization and SEC HPLC analysis (lanes 1 and 2).
    Figure Legend Snippet: D1 anaerobic protein purity. Protein was expressed in DH5α from the pASG-IBA35 vector A. Steps of D1 anaerobic purification process. Numbers above the gel mark fractions: 1 – before induction, 2- after induction, 3 – lysate, 4- pellet after centrifugation at 40 000 rcf, 5- NiNTA flow through, 6 – elution (this D1 anaerobic protein batch was used for EXAFS experiments). The marked band with mass 30 kDa corresponds to D1 protein. B. The purity of D1 anaerobic protein batch used for TSA, crystallization and SEC HPLC analysis (lanes 1 and 2).

    Techniques Used: Plasmid Preparation, Purification, Centrifugation, Flow Cytometry, Crystallization Assay, Size-exclusion Chromatography, High Performance Liquid Chromatography

    Related Articles

    Clone Assay:

    Article Title: Intracellular redistribution of neuronal peroxisomes in response to ACBD5 expression
    Article Snippet: .. Plasmids and antibodies All plasmids were cloned and amplified using DH5α Competent Cells (Invitrogen, Cat. No. 18265–017). .. The plasmids encoding EGFP-SKL, myc-ACBD5(human), FLAG-ACBD5(human)-FFAT, ACBD5(human)-ACB and FLAG-ACBD5(human)-WT were generated as described in a previous publication (16).

    Article Title: A Duplex PCR-Based Assay for Measuring the Amount of Bacterial Contamination in a Nucleic Acid Extract from a Culture of Free-Living Protists
    Article Snippet: .. The amplified sequences were cloned into a plasmid vector using the PGEM-T Easy Vector System (Promega) and Subcloning Efficiency DH5α Competent Cells (Invitrogen). .. The plasmids were extracted using a Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: In non-transplant patients with multiple myeloma, the pre-treatment level of clonotypic cells predicts event-free survival
    Article Snippet: .. The β2m and patient specific IgH VDJ amplicons were cloned using pGEM-T Easy Vector System I (Promega, Madison, WI, USA) and Subcloning Efficiency DH5α cells (Invitrogen, Carlsbad, CA, USA) per manufacturer’s instructions. .. Plasmids were isolated using QIAprep Spin Miniprep Kit (Qiagen Inc., Mississauga, Ontario, Canada) and the identity of the insert was confirmed by PCR with the primers used to generate the original amplicon.

    Article Title: Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor ▿ ▿ †
    Article Snippet: .. The SCO3287 - SCO3291 ( abeABCD and abeR ) gene cluster was excised from cosmid E15 (Table ) using KpnI, and the desired fragment was gel purified and cloned into pIJ2925 (Table ) using a rapid DNA ligation kit (Roche) prior to transformation into subcloning-efficiency DH5α E. coli cells (Invitrogen). .. The recombinant plasmid (pMC123) was isolated and digested with BglII before the resulting SCO3287 - SCO3291 -containing fragment was subcloned into the BamHI site of pWHM3, generating pMC120. pMC120 was passaged through the nonmethylating E. coli strain ET12567/pUZ8002 (Table ) prior to introduction into S. coelicolor .

    Transfection:

    Article Title: Chromosomal Polymorphism in the Sporothrix schenckii Complex
    Article Snippet: .. These plasmids were transfected into Subcloning Efficiency DH5-α Competent Cells (Invitrogen, USA) according to the manufacturer’s protocol. .. Plasmids were purified with the NucleoSpin Plasmid kit (Macherey-Nagel, Germany) and sequenced with the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA) and an ABI 3730 DNA Analyzer (Applied Biosystems, USA).

    Amplification:

    Article Title: Intracellular redistribution of neuronal peroxisomes in response to ACBD5 expression
    Article Snippet: .. Plasmids and antibodies All plasmids were cloned and amplified using DH5α Competent Cells (Invitrogen, Cat. No. 18265–017). .. The plasmids encoding EGFP-SKL, myc-ACBD5(human), FLAG-ACBD5(human)-FFAT, ACBD5(human)-ACB and FLAG-ACBD5(human)-WT were generated as described in a previous publication (16).

    Article Title: A Duplex PCR-Based Assay for Measuring the Amount of Bacterial Contamination in a Nucleic Acid Extract from a Culture of Free-Living Protists
    Article Snippet: .. The amplified sequences were cloned into a plasmid vector using the PGEM-T Easy Vector System (Promega) and Subcloning Efficiency DH5α Competent Cells (Invitrogen). .. The plasmids were extracted using a Qiaprep Spin Miniprep Kit (Qiagen).

    DNA Ligation:

    Article Title: Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor ▿ ▿ †
    Article Snippet: .. The SCO3287 - SCO3291 ( abeABCD and abeR ) gene cluster was excised from cosmid E15 (Table ) using KpnI, and the desired fragment was gel purified and cloned into pIJ2925 (Table ) using a rapid DNA ligation kit (Roche) prior to transformation into subcloning-efficiency DH5α E. coli cells (Invitrogen). .. The recombinant plasmid (pMC123) was isolated and digested with BglII before the resulting SCO3287 - SCO3291 -containing fragment was subcloned into the BamHI site of pWHM3, generating pMC120. pMC120 was passaged through the nonmethylating E. coli strain ET12567/pUZ8002 (Table ) prior to introduction into S. coelicolor .

    Subcloning:

    Article Title: Chromosomal Polymorphism in the Sporothrix schenckii Complex
    Article Snippet: .. These plasmids were transfected into Subcloning Efficiency DH5-α Competent Cells (Invitrogen, USA) according to the manufacturer’s protocol. .. Plasmids were purified with the NucleoSpin Plasmid kit (Macherey-Nagel, Germany) and sequenced with the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA) and an ABI 3730 DNA Analyzer (Applied Biosystems, USA).

    Article Title: A Duplex PCR-Based Assay for Measuring the Amount of Bacterial Contamination in a Nucleic Acid Extract from a Culture of Free-Living Protists
    Article Snippet: .. The amplified sequences were cloned into a plasmid vector using the PGEM-T Easy Vector System (Promega) and Subcloning Efficiency DH5α Competent Cells (Invitrogen). .. The plasmids were extracted using a Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: Deletion of TLR5 results in spontaneous colitis in mice
    Article Snippet: .. Subcloning Efficiency DH5α Chemically Competent Cells (Invitrogen) were used for the transformation. .. Transformants for inserts were screened on Luria-Bertani/ampicillin/IPTG/X-Gal plates and selected positive white colonies and grown overnight at 37°C by shaking; plasmid DNA was isolated (QIAGEN) and sequenced using M13 forward and reverse primers at Genomic Services, Agencourt Bioscience Corp.

    Article Title: In non-transplant patients with multiple myeloma, the pre-treatment level of clonotypic cells predicts event-free survival
    Article Snippet: .. The β2m and patient specific IgH VDJ amplicons were cloned using pGEM-T Easy Vector System I (Promega, Madison, WI, USA) and Subcloning Efficiency DH5α cells (Invitrogen, Carlsbad, CA, USA) per manufacturer’s instructions. .. Plasmids were isolated using QIAprep Spin Miniprep Kit (Qiagen Inc., Mississauga, Ontario, Canada) and the identity of the insert was confirmed by PCR with the primers used to generate the original amplicon.

    Article Title: Translational Regulation by an Intramolecular Stem-Loop Is Required for Intermolecular RNA Regulation of the par Addiction Module ▿ Addiction Module ▿ †
    Article Snippet: .. Transformation into E. coli was achieved with Subcloning Efficiency DH5α chemically competent cells (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor ▿ ▿ †
    Article Snippet: .. The SCO3287 - SCO3291 ( abeABCD and abeR ) gene cluster was excised from cosmid E15 (Table ) using KpnI, and the desired fragment was gel purified and cloned into pIJ2925 (Table ) using a rapid DNA ligation kit (Roche) prior to transformation into subcloning-efficiency DH5α E. coli cells (Invitrogen). .. The recombinant plasmid (pMC123) was isolated and digested with BglII before the resulting SCO3287 - SCO3291 -containing fragment was subcloned into the BamHI site of pWHM3, generating pMC120. pMC120 was passaged through the nonmethylating E. coli strain ET12567/pUZ8002 (Table ) prior to introduction into S. coelicolor .

    Purification:

    Article Title: Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor ▿ ▿ †
    Article Snippet: .. The SCO3287 - SCO3291 ( abeABCD and abeR ) gene cluster was excised from cosmid E15 (Table ) using KpnI, and the desired fragment was gel purified and cloned into pIJ2925 (Table ) using a rapid DNA ligation kit (Roche) prior to transformation into subcloning-efficiency DH5α E. coli cells (Invitrogen). .. The recombinant plasmid (pMC123) was isolated and digested with BglII before the resulting SCO3287 - SCO3291 -containing fragment was subcloned into the BamHI site of pWHM3, generating pMC120. pMC120 was passaged through the nonmethylating E. coli strain ET12567/pUZ8002 (Table ) prior to introduction into S. coelicolor .

    Transformation Assay:

    Article Title: Deletion of TLR5 results in spontaneous colitis in mice
    Article Snippet: .. Subcloning Efficiency DH5α Chemically Competent Cells (Invitrogen) were used for the transformation. .. Transformants for inserts were screened on Luria-Bertani/ampicillin/IPTG/X-Gal plates and selected positive white colonies and grown overnight at 37°C by shaking; plasmid DNA was isolated (QIAGEN) and sequenced using M13 forward and reverse primers at Genomic Services, Agencourt Bioscience Corp.

    Article Title: Translational Regulation by an Intramolecular Stem-Loop Is Required for Intermolecular RNA Regulation of the par Addiction Module ▿ Addiction Module ▿ †
    Article Snippet: .. Transformation into E. coli was achieved with Subcloning Efficiency DH5α chemically competent cells (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor ▿ ▿ †
    Article Snippet: .. The SCO3287 - SCO3291 ( abeABCD and abeR ) gene cluster was excised from cosmid E15 (Table ) using KpnI, and the desired fragment was gel purified and cloned into pIJ2925 (Table ) using a rapid DNA ligation kit (Roche) prior to transformation into subcloning-efficiency DH5α E. coli cells (Invitrogen). .. The recombinant plasmid (pMC123) was isolated and digested with BglII before the resulting SCO3287 - SCO3291 -containing fragment was subcloned into the BamHI site of pWHM3, generating pMC120. pMC120 was passaged through the nonmethylating E. coli strain ET12567/pUZ8002 (Table ) prior to introduction into S. coelicolor .

    Plasmid Preparation:

    Article Title: A Duplex PCR-Based Assay for Measuring the Amount of Bacterial Contamination in a Nucleic Acid Extract from a Culture of Free-Living Protists
    Article Snippet: .. The amplified sequences were cloned into a plasmid vector using the PGEM-T Easy Vector System (Promega) and Subcloning Efficiency DH5α Competent Cells (Invitrogen). .. The plasmids were extracted using a Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: In non-transplant patients with multiple myeloma, the pre-treatment level of clonotypic cells predicts event-free survival
    Article Snippet: .. The β2m and patient specific IgH VDJ amplicons were cloned using pGEM-T Easy Vector System I (Promega, Madison, WI, USA) and Subcloning Efficiency DH5α cells (Invitrogen, Carlsbad, CA, USA) per manufacturer’s instructions. .. Plasmids were isolated using QIAprep Spin Miniprep Kit (Qiagen Inc., Mississauga, Ontario, Canada) and the identity of the insert was confirmed by PCR with the primers used to generate the original amplicon.

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  • 96
    Thermo Fisher subcloning efficiency dh5α competent cells
    Subcloning Efficiency Dh5α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning efficiency dh5α competent cells/product/Thermo Fisher
    Average 96 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    subcloning efficiency dh5α competent cells - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

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