study umuc3  (ATCC)

Journal: Cancer Science

Article Title: Alix‐normalized exosomal programmed death‐ligand 1 analysis in urine enables precision monitoring of urothelial cancer

doi: 10.1111/cas.16106

Figure Lengend Snippet: Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from T24 CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.

Article Snippet: The cell lines used in this study (UMUC3, T24, RWPE, and A549) were obtained from ATCC.

Techniques: Expressing, Isolation, Microscopy, Transmission Assay, Binding Assay

Journal: Molecular Cancer Therapeutics

Article Title: Cyclooxygenase inhibitors in urinary bladder cancer:in vitroandin vivoeffects

doi: 10.1158/1535-7163.mct-05-0117

Figure Lengend Snippet: Figure 1. In vitro COX-2 expression in three human TCC cell lines (HT1376, RT4, and UMUC3) detected by Western blot as described in Materials and Methods. Actin confirms equal loading of protein.

Article Snippet: The cell lines used for these studies (HT1376, RT4, and UMUC3) were obtained from the American Type Culture Collection (Rockville, MD).

Techniques: In Vitro, Expressing, Western Blot

Journal: Molecular Cancer Therapeutics

Article Title: Cyclooxygenase inhibitors in urinary bladder cancer:in vitroandin vivoeffects

doi: 10.1158/1535-7163.mct-05-0117

Figure Lengend Snippet: Figure 2. Photomicrographs demonstrating COX-2 immunoreactivity in HT1376 cells in vitro (A) and in HT1376 xenografts in nu/nu mice (B). COX-2 expression is absent in UMUC3 cells in vitro (C) and in UMUC3 xenografts in nu/nu mice (D). COX-2 expression (note eosinophilic cytoplasmic staining in immunoreactive cells) was detected by immunocytochemistry (A and C) and immunohisto- chemistry (B and D).

Article Snippet: The cell lines used for these studies (HT1376, RT4, and UMUC3) were obtained from the American Type Culture Collection (Rockville, MD).

Techniques: In Vitro, Expressing, Staining, Immunocytochemistry, Immunohistochemistry

Journal: Molecular Cancer Therapeutics

Article Title: Cyclooxygenase inhibitors in urinary bladder cancer:in vitroandin vivoeffects

doi: 10.1158/1535-7163.mct-05-0117

Figure Lengend Snippet: Figure 3. Effects of celecoxib, piroxicam, and NS-398 (72-h exposure) on the growth of HT1376 urinary bladder cancer cells. Piroxicam and NS- 398 did not inhibit growth. Celecoxib had minimal inhibitory effects at 25 Amol/L and marked inhibitory effects at 50 and 100 Amol/L. Concentrations of celecoxib reached in humans are typically V5 Amol/L (25). Results with RT4 and UMUC3 cells were similar to those observed in HT1376 cells (data not shown). Experiments were repeated thrice.

Article Snippet: The cell lines used for these studies (HT1376, RT4, and UMUC3) were obtained from the American Type Culture Collection (Rockville, MD).

Techniques: