streptomycin  (Thermo Fisher)


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    Name:
    Streptomycin Sulfate
    Description:
    Streptomycin Sulfate is a water soluble antibiotic originally purified from Streptomyces griseus Streptomycin Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria Streptomycin Sulfate is highly active against gram negative with some activity against gram positive bacteria Gibco Streptomycin Sulfate is used alone or in combination with penicillin an antibiotic highly active against gram positive bacteria for the prevention of bacterial contamination of cell cultures The recommended working concentration ranges from 50 to 100 µg ml We offer a variety of antibiotics and antimycotics for cell culture applications Product UseFor Research Use Only Not intended for animal or human diagnostic or therapeutic use Dual Site cGMP ManufacturingFor supply chain continuity we manufacture Gibco Streptomycin Sulfate at two separate facilities located in Grand Island NY and Scotland UK Both sites are compliant with cGMP manufacturing requirements are certified to ISO 13485 and are registered with the FDA as medical device manufacturers
    Catalog Number:
    11860038
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cloning|Microbiological Culture|Transformation
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    Structured Review

    Thermo Fisher streptomycin
    Streptomycin Sulfate is a water soluble antibiotic originally purified from Streptomyces griseus Streptomycin Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria Streptomycin Sulfate is highly active against gram negative with some activity against gram positive bacteria Gibco Streptomycin Sulfate is used alone or in combination with penicillin an antibiotic highly active against gram positive bacteria for the prevention of bacterial contamination of cell cultures The recommended working concentration ranges from 50 to 100 µg ml We offer a variety of antibiotics and antimycotics for cell culture applications Product UseFor Research Use Only Not intended for animal or human diagnostic or therapeutic use Dual Site cGMP ManufacturingFor supply chain continuity we manufacture Gibco Streptomycin Sulfate at two separate facilities located in Grand Island NY and Scotland UK Both sites are compliant with cGMP manufacturing requirements are certified to ISO 13485 and are registered with the FDA as medical device manufacturers
    https://www.bioz.com/result/streptomycin/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptomycin - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: BRCA1/BARD1-dependent ubiquitination of NF2 regulates Hippo-YAP1 signaling
    Article Snippet: HEK293T, HEK293A (human embryonic kidney cell), U2OS (human osteosarcoma cell), and H-1299 (human non–small-cell lung carcinoma cell) were maintained in DMEM (Gibco, Invitrogen) supplemented with glutamine, 10% FCS, 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen) at 37 °C with 5% CO2 . .. MCF10A cells (human mammary epithelial cell) were cultured in DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Invitrogen), 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 10 μg/mL insulin, 100 ng/mL cholera toxin, and 100 μg/mL streptomycin (Invitrogen) at 37 °C with 5% CO2 . ..

    Article Title: Cyclin G2 promotes cell cycle arrest in breast cancer cells responding to fulvestrant and metformin and correlates with patient survival
    Article Snippet: .. For estrogen depletion experiments, plated cells were washed twice with 1x PBS (Gibco) and cultured for indicated periods of time in phenol red free MEM medium containing 10% heat inactivated charcoal-dextran (CD)-treated FBS, 2 mM L -glutamine, 1 mM sodium pyruvate, 100 units/mL penicillin and 100 μg/mL streptomycin sulfate, 10 μg/mL bovine insulin and 10 mM HEPES (Gibco). ..

    Article Title: TACR3 mutations disrupt NK3R function through distinct mechanisms in GnRH-deficient patients
    Article Snippet: The sequences of the mutant cDNAs were confirmed by bidirectional sequencing. .. Both HEK293 and COS-7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (Invitrogen, Carlsbad, CA, USA) and supplemented with 10% (v/v) fetal bovine serum (Omega, Tarzana, CA, USA) in 5% CO2 humidified air at 37°C. ..

    other:

    Article Title: The phenotypic and molecular characteristics of antimicrobial resistance of Salmonella enterica subsp. enterica serovar Typhimurium in Henan Province, China
    Article Snippet: Antimicrobial susceptibility testing The susceptibility of each isolate to the following 13 antimicrobial agents was evaluated: ceftriaxone (AXO), cefoxitin (FOX), ampicillin (AMP), amoxicillin/clavulanic acid, 2:1 ratio (AUG2), CIP, nalidixic acid (NAL), AZI, tetracycline (TET), chloramphenicol (CHL), trimethoprim/sulfamethoxazole (SXT), sulfisoxazole (FIS), gentamicin (GEN), and streptomycin (STR).

    Modification:

    Article Title: TACR3 mutations disrupt NK3R function through distinct mechanisms in GnRH-deficient patients
    Article Snippet: The sequences of the mutant cDNAs were confirmed by bidirectional sequencing. .. Both HEK293 and COS-7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (Invitrogen, Carlsbad, CA, USA) and supplemented with 10% (v/v) fetal bovine serum (Omega, Tarzana, CA, USA) in 5% CO2 humidified air at 37°C. ..

    Article Title: Expression of the Hutchinson-Gilford Progeria Mutation Leads to Aberrant Dentin Formation
    Article Snippet: The specimen surfaces were sputter-coated with platinum after drying and examined with a scanning electron microscope (JSM-6400; JEOL, Tokyo, Japan) under 20-kV conditions. .. MDPC-23 cells , a murine dental papilla cell line, were maintained in Dulbecco’s modified Eagle medium (DMEM; Invitrogen, New York, NY, USA) with 10% fetal bovine serum (FBS; Invitrogen), and 100 IU/ml penicillin-100 μg/ml streptomycin (Invitrogen). .. For mouse dental pulp cells (DPCs), the pulp of mandibular first and second molars from 7- to 8-day-old mice was isolated and digested in a solution of 3 mg/mL collagenase type I (Worthington Biochemical Corp., Freehold, NJ, USA) and 4 mg/mL dispase (Boehringer, Mannheim, Germany) in a serum-free alpha modification of Eagle’s medium (α-MEM; Invitrogen) for 1 h at 37 °C.

    Purification:

    Article Title: Emergence of stealth polymorphs that escape α-synuclein amyloid monitoring, take over and acutely spread in neurons
    Article Snippet: The site-specific non-phosphorylable mutant S129A was obtained by site-directed mutagenesis of pET24-α-Syn. .. α-syn purification Pellet was thaw in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA and 1 mM PMSF, Pierce Complete EDTA-free protease inhibitors tablet (Thermofisher) buffer and sonicated 3 times 45 sec (Bandelin Sonoplus – VS70T probe) previous to be centrifuged. .. Streptomycin sulphate was added to supernatant to final concentration of 10 mg/mL and the solution was stirring for 15 minutes at 4 °C then centrifuge.

    Sonication:

    Article Title: Emergence of stealth polymorphs that escape α-synuclein amyloid monitoring, take over and acutely spread in neurons
    Article Snippet: The site-specific non-phosphorylable mutant S129A was obtained by site-directed mutagenesis of pET24-α-Syn. .. α-syn purification Pellet was thaw in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA and 1 mM PMSF, Pierce Complete EDTA-free protease inhibitors tablet (Thermofisher) buffer and sonicated 3 times 45 sec (Bandelin Sonoplus – VS70T probe) previous to be centrifuged. .. Streptomycin sulphate was added to supernatant to final concentration of 10 mg/mL and the solution was stirring for 15 minutes at 4 °C then centrifuge.

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    Thermo Fisher mesc medium
    SIRT1 expression during iPSC formation and differentiation of ESCs and iPSCs in mouse model. (A) Temporal expression of Sirt1 mRNA on day 0 to day 20 after DOX treatment. MEF without DOX on day 20 (20-DOX) and <t>mESC</t> were included. (B) Western blotting analysis of SIRT1, OCT4 and PCNA in 2°F/1B MEF without (-DOX) and with (+DOX) DOX treatment for 15 days, serially passaged iPSC from passages 4 (P4), 5–7 (P5, P6, P7) and differentiated colonies at passage 4 (Diff-P4). (C) The relative expression levels of SIRT1 protein in MEF, <t>miPSC</t> and mESC. (D) Relative SIRT1 protein expressions in embryoid bodies collected from mESC and miPSC on days 2, 5, 8, 11, 14 and 17 after differentiation. D0 are the undifferentiated cell control. *p
    Mesc Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mesc medium/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    97
    Thermo Fisher penicillin g
    Suggested diagnosis approach by disk diffusion method or Vitek 2® method to determine susceptibility of S. epidermidis to <t>penicillin</t> G
    Penicillin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin g/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin g - by Bioz Stars, 2021-04
    97/100 stars
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    N/A
    Perform accurate Mtb susceptibility testing utilizing the antituberculosis drug streptomycin with Thermo Scientific VersaTREK Myco Streptomycin
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    SIRT1 expression during iPSC formation and differentiation of ESCs and iPSCs in mouse model. (A) Temporal expression of Sirt1 mRNA on day 0 to day 20 after DOX treatment. MEF without DOX on day 20 (20-DOX) and mESC were included. (B) Western blotting analysis of SIRT1, OCT4 and PCNA in 2°F/1B MEF without (-DOX) and with (+DOX) DOX treatment for 15 days, serially passaged iPSC from passages 4 (P4), 5–7 (P5, P6, P7) and differentiated colonies at passage 4 (Diff-P4). (C) The relative expression levels of SIRT1 protein in MEF, miPSC and mESC. (D) Relative SIRT1 protein expressions in embryoid bodies collected from mESC and miPSC on days 2, 5, 8, 11, 14 and 17 after differentiation. D0 are the undifferentiated cell control. *p

    Journal: PLoS ONE

    Article Title: Sirtuin 1 Facilitates Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts through the miR-34a and p53 Pathways

    doi: 10.1371/journal.pone.0045633

    Figure Lengend Snippet: SIRT1 expression during iPSC formation and differentiation of ESCs and iPSCs in mouse model. (A) Temporal expression of Sirt1 mRNA on day 0 to day 20 after DOX treatment. MEF without DOX on day 20 (20-DOX) and mESC were included. (B) Western blotting analysis of SIRT1, OCT4 and PCNA in 2°F/1B MEF without (-DOX) and with (+DOX) DOX treatment for 15 days, serially passaged iPSC from passages 4 (P4), 5–7 (P5, P6, P7) and differentiated colonies at passage 4 (Diff-P4). (C) The relative expression levels of SIRT1 protein in MEF, miPSC and mESC. (D) Relative SIRT1 protein expressions in embryoid bodies collected from mESC and miPSC on days 2, 5, 8, 11, 14 and 17 after differentiation. D0 are the undifferentiated cell control. *p

    Article Snippet: L4 and miPSC were cultured in mESC medium [DMEM with high glucose, 100 units/ml penicillin and 100 µg/ml streptomycin (Gibco, Life Technologies), 0.1 mM MEM non-essential amino-acids (Gibco), sodium pyruvate (110 mg/L, Gibco), 50 µM beta-mercaptoethanol, 15% FBS (Gibco) and 1000 units/ml LIF (Millipore).

    Techniques: Expressing, Western Blot

    TPUF represses endogenously expressed VEGFA gene in HEK293 cell line. (a) Mutations and binding sequences of TPUFs designed for VEGFA 3′ UTR recognition. Black, WT modules and corresponding RNA bases. Red, mutant modules and corresponding RNA bases. Blue, a mismatch in the recognition sequence. Ct, C-terminus, Nt, N-terminus of the protein. (b) The graph demonstrates inhibition of hypoxia-induced VEGFA expression in cells transfected with engineered TPUFs VEGF3 and VEGF7. In hypoxic (+) cultures, VEGFA expression was induced with 500 μM CoCl 2 24 hours after transfection and then cultivated for 24 hours. Secreted VEGFA levels measured by ELISA were normalized to total protein amounts from lysed cells measured by Bradford Assay. Data represented as mean ± SD: n.s., not significant, *P ≤ 0.05, **P ≤ 0.01, (n = 3, t test). (c) The graph demonstrates inhibition of DHB-induced VEGFA expression in cells transfected with TPUFs WT, VEGF1, VEGF3, and VEGF7. HEK293 cells with the integrated V24P-GS60 transcriptional activator of endogenous VEGFA promoter were treated with 100 nM DHB 24 hours after TPUF transfection and then cultivated for 24 hours. ELISA and normalization to total protein amounts as in the previous panel. Data represented as mean ± SD: n.s., not significant, *P ≤ 0.05. (d) Western blot of effector proteins using anti-Flag antibody shows greater expression of TPUFs VEGF3 and VEGF7 compared with TPUF (WT) and other mutants. Anti-α–tubulin antibody was used as a loading control.

    Journal: Journal of Biological Engineering

    Article Title: Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA

    doi: 10.1186/1754-1611-8-7

    Figure Lengend Snippet: TPUF represses endogenously expressed VEGFA gene in HEK293 cell line. (a) Mutations and binding sequences of TPUFs designed for VEGFA 3′ UTR recognition. Black, WT modules and corresponding RNA bases. Red, mutant modules and corresponding RNA bases. Blue, a mismatch in the recognition sequence. Ct, C-terminus, Nt, N-terminus of the protein. (b) The graph demonstrates inhibition of hypoxia-induced VEGFA expression in cells transfected with engineered TPUFs VEGF3 and VEGF7. In hypoxic (+) cultures, VEGFA expression was induced with 500 μM CoCl 2 24 hours after transfection and then cultivated for 24 hours. Secreted VEGFA levels measured by ELISA were normalized to total protein amounts from lysed cells measured by Bradford Assay. Data represented as mean ± SD: n.s., not significant, *P ≤ 0.05, **P ≤ 0.01, (n = 3, t test). (c) The graph demonstrates inhibition of DHB-induced VEGFA expression in cells transfected with TPUFs WT, VEGF1, VEGF3, and VEGF7. HEK293 cells with the integrated V24P-GS60 transcriptional activator of endogenous VEGFA promoter were treated with 100 nM DHB 24 hours after TPUF transfection and then cultivated for 24 hours. ELISA and normalization to total protein amounts as in the previous panel. Data represented as mean ± SD: n.s., not significant, *P ≤ 0.05. (d) Western blot of effector proteins using anti-Flag antibody shows greater expression of TPUFs VEGF3 and VEGF7 compared with TPUF (WT) and other mutants. Anti-α–tubulin antibody was used as a loading control.

    Article Snippet: The cells were induced 24 h after transfection with 100 nM DHB, in the presence of pen /strep (Gibco).

    Techniques: Binding Assay, Mutagenesis, Sequencing, Inhibition, Expressing, Transfection, Enzyme-linked Immunosorbent Assay, Bradford Assay, Western Blot

    eIF4E overexpression correlates with increased HA synthesis. ( A ) Fluorescence staining of HA (in green) using biotinylated HA-binding protein with streptavidin-FITC in U2Os cells overexpressing eIF4E, S53A mutant or vector control in the presence or absence of Streptomyces Hyaluronidase treatment. DAPI is in blue. Note cell surface expression of HA in response to eIF4E overexpression. All confocal settings are identical between specimens and thus lower signal is indicative of less HA. A × 40 objective with no digital zoom was used. ( B ) 2x digital zoom in confocal images of HA from part ( A ). ( C ) Quantification of fluorophore-assisted carbohydrate electrophoresis (FACE) gels (Sup Figure 1e f ) for HA levels in U2Os cells expressing eIF4E, S53A mutant or vector control, and U2Os cells overexpressing eIF4E following HAS3/eIF4E knockdown or pharmacological inhibition with ribavirin (Rib). ( D ) Fluorescence staining of HA (in green) following siRNA to eIF4E or ribavirin treatment in U2Os cells overexpressing eIF4E. DAPI is in blue. A × 63 objective with no digital zoom used. For bar graphs, the mean ± SD are shown. Experiments were carried out in triplicate, at least three independent times. **p

    Journal: eLife

    Article Title: The eukaryotic translation initiation factor eIF4E harnesses hyaluronan production to drive its malignant activity

    doi: 10.7554/eLife.29830

    Figure Lengend Snippet: eIF4E overexpression correlates with increased HA synthesis. ( A ) Fluorescence staining of HA (in green) using biotinylated HA-binding protein with streptavidin-FITC in U2Os cells overexpressing eIF4E, S53A mutant or vector control in the presence or absence of Streptomyces Hyaluronidase treatment. DAPI is in blue. Note cell surface expression of HA in response to eIF4E overexpression. All confocal settings are identical between specimens and thus lower signal is indicative of less HA. A × 40 objective with no digital zoom was used. ( B ) 2x digital zoom in confocal images of HA from part ( A ). ( C ) Quantification of fluorophore-assisted carbohydrate electrophoresis (FACE) gels (Sup Figure 1e f ) for HA levels in U2Os cells expressing eIF4E, S53A mutant or vector control, and U2Os cells overexpressing eIF4E following HAS3/eIF4E knockdown or pharmacological inhibition with ribavirin (Rib). ( D ) Fluorescence staining of HA (in green) following siRNA to eIF4E or ribavirin treatment in U2Os cells overexpressing eIF4E. DAPI is in blue. A × 63 objective with no digital zoom used. For bar graphs, the mean ± SD are shown. Experiments were carried out in triplicate, at least three independent times. **p

    Article Snippet: Mono-Mac-6 (MM6) cells (obtained from DSMZ, Cat# ACC 124) were maintained in RPMI 1640 (ThermoFisher Scientific) supplemented with 10% FBS, 1% penicillin-streptavidin, 1% MEM non-essential amino acids (ThermoFisher Scientific, Cat# 11140076) and 10 µg/ml recombinant human insulin (Sigma Aldrich, Cat# 91077C).

    Techniques: Over Expression, Fluorescence, Staining, Binding Assay, Mutagenesis, Plasmid Preparation, Expressing, Electrophoresis, Inhibition

    Suggested diagnosis approach by disk diffusion method or Vitek 2® method to determine susceptibility of S. epidermidis to penicillin G

    Journal: BMC Microbiology

    Article Title: Performance of penicillinase detection tests in Staphylococcus epidermidis: comparison of different phenotypic methods

    doi: 10.1186/s12866-020-01929-x

    Figure Lengend Snippet: Suggested diagnosis approach by disk diffusion method or Vitek 2® method to determine susceptibility of S. epidermidis to penicillin G

    Article Snippet: Diffusion method - reading inhibition diameters The disk diffusion method was performed on Mueller-Hinton agar plates (Oxoid®, Dardilly, France) with a 0.5 Mac Farland bacterial suspension, and by using a 1 unit disk of penicillin G (Oxoid®, Dardilly, France) according to EUCAST recommendations [ ].

    Techniques: Diffusion-based Assay

    a Dispersal of diffusion diameters according to the presence of the bla Z gene. b Distribution of Penicillin G MIC (Vitek 2®) for 182 S. epidermidis isolates according to the presence of bla Z gene. Black bars represent blaZ positive S. epidermidis isolates and grey bars represent blaZ negative S. epidermidis isolates

    Journal: BMC Microbiology

    Article Title: Performance of penicillinase detection tests in Staphylococcus epidermidis: comparison of different phenotypic methods

    doi: 10.1186/s12866-020-01929-x

    Figure Lengend Snippet: a Dispersal of diffusion diameters according to the presence of the bla Z gene. b Distribution of Penicillin G MIC (Vitek 2®) for 182 S. epidermidis isolates according to the presence of bla Z gene. Black bars represent blaZ positive S. epidermidis isolates and grey bars represent blaZ negative S. epidermidis isolates

    Article Snippet: Diffusion method - reading inhibition diameters The disk diffusion method was performed on Mueller-Hinton agar plates (Oxoid®, Dardilly, France) with a 0.5 Mac Farland bacterial suspension, and by using a 1 unit disk of penicillin G (Oxoid®, Dardilly, France) according to EUCAST recommendations [ ].

    Techniques: Diffusion-based Assay