Article Title: Homing Choices of Breast Cancer Cells Revealed by Tissue Specific Invasion and Extravasation Lab-on-a-chip Platforms
Figure Lengend Snippet: Extravasation of metastatic breast cancer cells into lung, liver and breast microenvironments. a) 3D schematic of the EX-chip. Zoom-in image of 3D EX-chip showing the side 2 view (Cancer cells: Red, Endothelial cells: Green, Direction of extravasation: Blue arrow). b) Representative Z-stack images showing side 2 views of endothelial monolayer formation by HUVEC-C cells (green) and extravasated (arrow head) or associated (arrow) MDA-MB-231 cells (red) into lung, liver or breast microenvironments generated by WI-38, BRL-3A and MCF-10A cell lines, respectively (Scale bar: 200 μm). c) The number of extravasated and associated MDA-MB-231 cells. Each black dot represents the cell number for one post gap within the EX-chip, while the red dot is the average number of cells for each condition (n=9).
Article Snippet: MDA-MB-231, its derivatives and MCF-7 were cultured in DMEM high glucose (11965092, Gibco) with Fetal Bovine Serum (FBS, 10%) (A3840001, Gibco) and Penicillin/Streptomycin (15070063, Gibco, 1%); MCF-10A was cultured in DMEM-F12 high glucose (11330057, Gibco) with Horse Serum (04-004-1A, Biological Industries, 5%), Insulin (I9278, Sigma, 10 μg mL-1 ), Cholera Toxin (C8052, Sigma, 100 ng mL-1 ), EGF (E9644, Sigma, 20 ng mL-1 ), Hydrocortisone (H0888, Sigma, 0.5 μg mL-1 ) and Penicillin/Streptomycin (1%).
Techniques: Chromatin Immunoprecipitation, Multiple Displacement Amplification, Generated