streptavidin peroxidase conjugate  (Millipore)


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    Millipore streptavidin peroxidase conjugate
    Streptavidin Peroxidase Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin peroxidase conjugate/product/Millipore
    Average 95 stars, based on 242 article reviews
    Price from $9.99 to $1999.99
    streptavidin peroxidase conjugate - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Enhancement of soluble CD28 levels in the serum of Graves’ disease
    Article Snippet: Paragraph title: Detection of soluble CD28 by ELISA ... After washing twice, soluble CD28 captured by coating the antibody was incubated with biotin-conjugated anti-CD28 mAb (prepared in our lab, clone 2D5), followed by HRP-streptavidin (Sigma, MO, USA) incubation for 1 hour at 37°C and then reacted with the substrate TMB (KPL, UK).

    Article Title: HSP90 inhibitors decrease AID levels and activity in mice and in human cells
    Article Snippet: Briefly, ELISA plates were coated with 100 μl of 50 μg/mL NP26 -BSA, for capturing total anti-NP antibodies, or NP4 -BSA, for capturing high affinity anti-NP antibodies. .. IgG1 was detected using 50 μl biotin-anti-IgG1 for 2 h followed by 50 μl of HRP-streptavidin for 1 h and visualized using 2,2′-Azino-bis(3-ethybenzothiazoline-6-sulfonic acid) (Sigma), and OD read at 405 nm.

    Article Title: Mycoplasma promotes malignant transformation in vivo, and its DnaK, a bacterial chaperon protein, has broad oncogenic properties
    Article Snippet: Paragraph title: ELISA-Based Assay for Detection of PARP1 Activity. ... The wells then were incubated first with HRP-Streptavidin (Sigma-Aldrich) for 1 h at room temperature and then with a colorimetric substrate, following two washes with 1× PBS+0.1% Triton X-100 and two washes with 1× PBS.

    Article Title: Shortened Engineered Human Antibody CH2 Domains
    Article Snippet: To confirm the result, a biotin-conjugated commercial mouse anti-human CH2 monoclonal antibody (AbD Serotec) was also used for ELISA at concentrations of 0–10 μg/ml. .. HRP-streptavidin (Sigma) was used for detection of biotinylated mouse anti-human CH2 antibody.

    SDS-Gel:

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: .. To confirm the biotin moiety in biotin-BDNF, proteins on SDS gel were transferred to a PVDF membrane and blotted with HRP-streptavidin. showed that both HPLC peaks of biotin-BDNF bind tightly to streptavidin, confirming the presence of biotin tags. ..

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: .. To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152). .. For immunoprecipitation, 3T3 cells expressing TrkB (a generous gift from Dr. Chengbiao Wu, UC San Diago) were cultured in a 15 cm dish till ∼70% confluency.

    Incubation:

    Article Title: β2 adrenergic agonist attenuates house dust mite-induced allergic airway inflammation through dendritic cells
    Article Snippet: Each well was filled with a blocking solution of 1% BSA (Sigma-Aldrich) in PBS, and incubated for 1 hour. .. After washing, IgG1 bound to the plate was detected using biotin-labeled rat anti-mouse IgG1 (Bio-Rad AbD Serotec Ltd, Oxford UK), HRP-streptavidin (Sigma-Aldrich), and 3,3’,5,5’-tetramethylbenzidine (TMB, Invitrogen, CA).

    Article Title: Enhancement of soluble CD28 levels in the serum of Graves’ disease
    Article Snippet: .. After washing twice, soluble CD28 captured by coating the antibody was incubated with biotin-conjugated anti-CD28 mAb (prepared in our lab, clone 2D5), followed by HRP-streptavidin (Sigma, MO, USA) incubation for 1 hour at 37°C and then reacted with the substrate TMB (KPL, UK). ..

    Article Title: HSP90 inhibitors decrease AID levels and activity in mice and in human cells
    Article Snippet: Serial dilutions of mouse serum were loaded in duplicate wells and incubated overnight at 4°C. .. IgG1 was detected using 50 μl biotin-anti-IgG1 for 2 h followed by 50 μl of HRP-streptavidin for 1 h and visualized using 2,2′-Azino-bis(3-ethybenzothiazoline-6-sulfonic acid) (Sigma), and OD read at 405 nm.

    Article Title: Mycoplasma promotes malignant transformation in vivo, and its DnaK, a bacterial chaperon protein, has broad oncogenic properties
    Article Snippet: .. The wells then were incubated first with HRP-Streptavidin (Sigma-Aldrich) for 1 h at room temperature and then with a colorimetric substrate, following two washes with 1× PBS+0.1% Triton X-100 and two washes with 1× PBS. ..

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: .. To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152). .. For immunoprecipitation, 3T3 cells expressing TrkB (a generous gift from Dr. Chengbiao Wu, UC San Diago) were cultured in a 15 cm dish till ∼70% confluency.

    Article Title: Heligmosomoides polygyrus elicits a dominant non-protective antibody response directed against restricted glycan and peptide epitopes
    Article Snippet: .. Alternatively, IgA blots were incubated with biotinylated anti-mouse IgA (1/500, Invitrogen ) followed by HRP-streptavidin (1/2000, Sigma), and developed as above. ..

    Article Title: A Novel Method for Producing Mono-biotinylated, Biologically Active Neurotrophic Factors: An Essential Reagent for Single Molecule Study of Axonal Transport
    Article Snippet: HRP-streptavidin was from Chemicon (San Diego, CA, Cat# 18-152). .. For precipitation of proteins with trichloroacetic acid (TCA from Sigma, Cat# T6399), 100% ice-cold TCA was added to the supernatant to a final concentration of 5-7% and incubated on ice for 20 min.

    Activity Assay:

    Article Title: Mycoplasma promotes malignant transformation in vivo, and its DnaK, a bacterial chaperon protein, has broad oncogenic properties
    Article Snippet: Paragraph title: ELISA-Based Assay for Detection of PARP1 Activity. ... The wells then were incubated first with HRP-Streptavidin (Sigma-Aldrich) for 1 h at room temperature and then with a colorimetric substrate, following two washes with 1× PBS+0.1% Triton X-100 and two washes with 1× PBS.

    Article Title: Ubiquitin Receptor RPN13 Mediates the Inhibitory Interaction of Diphenyldihaloketones CLEFMA and EF24 With the 26S Proteasome
    Article Snippet: Specific fluorogenic peptide substrates for proteasome activity assays were purchased from Enzo Life Sciences (Farmingdale, NY). .. Anti-actin primary antibody, horse radish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody, and HRP-streptavidin were from Sigma-Aldrich (St. Louis, MO).

    Solubility:

    Article Title: Genome-Wide Analysis of PDZ Domain Binding Reveals Inherent Functional Overlap within the PDZ Interaction Network
    Article Snippet: PDZ domain binding assay Biotinylated peptides were designed according to Stifler et al. with an N-terminal tri-peptide (NNG) to increase solubility. .. For the binding assay, biotinylated peptides (4 µM) (purchased from United Peptide Corporation, UPC) were used, which were detected with HRP-Streptavidin and HRP substrate 3,3,5,5-Tetramethylbenzidine (TMB) (Sigma).

    Expressing:

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152). .. For immunoprecipitation, 3T3 cells expressing TrkB (a generous gift from Dr. Chengbiao Wu, UC San Diago) were cultured in a 15 cm dish till ∼70% confluency.

    Western Blot:

    Article Title: 4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils
    Article Snippet: .. The blotted membrane was blocked in 2% Block Ace Solution (Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan) for 1 h, probed with the mouse anti-GAPDH mAb (16 ng/mL) or rabbit anticathepsin G pAb (0.5 μ g/mL) for 2 h, and then visualized using the biotinylated antimouse or antirabbit immunoglobulins pAb, HRP-streptavidin, and Immobilon Western Chemiluminescent HRP Substrate (Millipore Co.). .. A LAS-1000 UV mini F85 Luminoimage Analyzer (Fujifilm Co., Tokyo, Japan) was used for chemiluminescence detection.

    Article Title: Combination of TRAIL with Bortezomib Shifted Apoptotic Signaling from DR4 to DR5 Death Receptor by Selective Internalization and Degradation of DR4
    Article Snippet: .. For western blot biotinylated goat antibodies to TRAIL death and decoy receptors were purchased from R & D Systems (Minneapolis, USA), HRP streptavidin and antibodies to actin were from Sigma-Aldrich (St. Lotus, USA). .. Recombinant TRAIL preparation Wild type and mutant variant DR5-B (containing 6 amino acid substitutions Y189N, R191K, Q193R, H264R, I266L and D269H) of TRAIL (114–281) were expressed in E. coli BL21(DE3) strain and purified as we previously described with some modifications .

    Article Title: Tubulovesicular Structures within Vesicular Stomatitis Virus G Protein-Pseudotyped Lentiviral Vector Preparations Carry DNA and Stimulate Antiviral Responses via Toll-Like Receptor 9 ▿
    Article Snippet: Paragraph title: Western blotting. ... Secondary reagents were horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Santa Cruz Biotechnology) or HRP-streptavidin (Sigma).

    Article Title: Ubiquitin Receptor RPN13 Mediates the Inhibitory Interaction of Diphenyldihaloketones CLEFMA and EF24 With the 26S Proteasome
    Article Snippet: Anti-actin primary antibody, horse radish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody, and HRP-streptavidin were from Sigma-Aldrich (St. Louis, MO). .. The immunoblots were imaged using Ultraquant image acquisition machine (Claremont, CA).

    Article Title: Heligmosomoides polygyrus elicits a dominant non-protective antibody response directed against restricted glycan and peptide epitopes
    Article Snippet: Paragraph title: 1D and 2D gel electrophoresis and Western blotting ... Alternatively, IgA blots were incubated with biotinylated anti-mouse IgA (1/500, Invitrogen ) followed by HRP-streptavidin (1/2000, Sigma), and developed as above.

    High Performance Liquid Chromatography:

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: .. To confirm the biotin moiety in biotin-BDNF, proteins on SDS gel were transferred to a PVDF membrane and blotted with HRP-streptavidin. showed that both HPLC peaks of biotin-BDNF bind tightly to streptavidin, confirming the presence of biotin tags. ..

    Silver Staining:

    Article Title: 4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils
    Article Snippet: For silver staining, 12% polyacrylamide gels were stained using a Wako Silver Stain II Kit (Wako Pure Chemical Industries Ltd.). .. The blotted membrane was blocked in 2% Block Ace Solution (Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan) for 1 h, probed with the mouse anti-GAPDH mAb (16 ng/mL) or rabbit anticathepsin G pAb (0.5 μ g/mL) for 2 h, and then visualized using the biotinylated antimouse or antirabbit immunoglobulins pAb, HRP-streptavidin, and Immobilon Western Chemiluminescent HRP Substrate (Millipore Co.).

    Immunoprecipitation:

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152). .. For immunoprecipitation, 3T3 cells expressing TrkB (a generous gift from Dr. Chengbiao Wu, UC San Diago) were cultured in a 15 cm dish till ∼70% confluency.

    Cell Culture:

    Article Title: Combination of TRAIL with Bortezomib Shifted Apoptotic Signaling from DR4 to DR5 Death Receptor by Selective Internalization and Degradation of DR4
    Article Snippet: Paragraph title: Cell culture and reagents ... For western blot biotinylated goat antibodies to TRAIL death and decoy receptors were purchased from R & D Systems (Minneapolis, USA), HRP streptavidin and antibodies to actin were from Sigma-Aldrich (St. Lotus, USA).

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152). .. For immunoprecipitation, 3T3 cells expressing TrkB (a generous gift from Dr. Chengbiao Wu, UC San Diago) were cultured in a 15 cm dish till ∼70% confluency.

    Recombinant:

    Article Title: Enhancement of soluble CD28 levels in the serum of Graves’ disease
    Article Snippet: After washing, recombinant human CD28 protein (R & D Systems, Abingdon, UK) and samples were triplicately added and incubated for 2 hours at 37˚C. .. After washing twice, soluble CD28 captured by coating the antibody was incubated with biotin-conjugated anti-CD28 mAb (prepared in our lab, clone 2D5), followed by HRP-streptavidin (Sigma, MO, USA) incubation for 1 hour at 37°C and then reacted with the substrate TMB (KPL, UK).

    Isolation:

    Article Title: Shortened Engineered Human Antibody CH2 Domains
    Article Snippet: Anti-CH2 Fab m01m1 identified from a human Fab naïve library ( ) by panning against isolated CH2 expressed in E. coli , which recognized the conformational epitope of CH2 (data not shown), was added at concentrations of 0.064–200 μg/ml. .. HRP-streptavidin (Sigma) was used for detection of biotinylated mouse anti-human CH2 antibody.

    Mouse Assay:

    Article Title: HSP90 inhibitors decrease AID levels and activity in mice and in human cells
    Article Snippet: Mice were immunized with NP18 -CGG (Biosearch Technologies) in Imject Alum adjuvant (Thermo Scientific) intraperitoneally. .. IgG1 was detected using 50 μl biotin-anti-IgG1 for 2 h followed by 50 μl of HRP-streptavidin for 1 h and visualized using 2,2′-Azino-bis(3-ethybenzothiazoline-6-sulfonic acid) (Sigma), and OD read at 405 nm.

    Blocking Assay:

    Article Title: 4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils
    Article Snippet: .. The blotted membrane was blocked in 2% Block Ace Solution (Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan) for 1 h, probed with the mouse anti-GAPDH mAb (16 ng/mL) or rabbit anticathepsin G pAb (0.5 μ g/mL) for 2 h, and then visualized using the biotinylated antimouse or antirabbit immunoglobulins pAb, HRP-streptavidin, and Immobilon Western Chemiluminescent HRP Substrate (Millipore Co.). .. A LAS-1000 UV mini F85 Luminoimage Analyzer (Fujifilm Co., Tokyo, Japan) was used for chemiluminescence detection.

    Article Title: β2 adrenergic agonist attenuates house dust mite-induced allergic airway inflammation through dendritic cells
    Article Snippet: Each well was filled with a blocking solution of 1% BSA (Sigma-Aldrich) in PBS, and incubated for 1 hour. .. After washing, IgG1 bound to the plate was detected using biotin-labeled rat anti-mouse IgG1 (Bio-Rad AbD Serotec Ltd, Oxford UK), HRP-streptavidin (Sigma-Aldrich), and 3,3’,5,5’-tetramethylbenzidine (TMB, Invitrogen, CA).

    Article Title: Tubulovesicular Structures within Vesicular Stomatitis Virus G Protein-Pseudotyped Lentiviral Vector Preparations Carry DNA and Stimulate Antiviral Responses via Toll-Like Receptor 9 ▿
    Article Snippet: After blocking in 5% bovine serum albumin, the membrane was stained with mouse anti-VSV-G (MAb P5D4 ( ) (Cancer Research UK), mouse anti-GFP (MAb GFP 3E1; Cancer Research UK), mouse anti-β-actin (MAb clone AC-15; Sigma), or biotinylated mouse anti-HIVp24 (Aalto). .. Secondary reagents were horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Santa Cruz Biotechnology) or HRP-streptavidin (Sigma).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: A Novel Method for Producing Mono-biotinylated, Biologically Active Neurotrophic Factors: An Essential Reagent for Single Molecule Study of Axonal Transport
    Article Snippet: Rabbit monoclonal antibody against pAkt was from Epitomics Inc (Cat2214-1). .. HRP-streptavidin was from Chemicon (San Diego, CA, Cat# 18-152).

    Purification:

    Article Title: Ubiquitin Receptor RPN13 Mediates the Inhibitory Interaction of Diphenyldihaloketones CLEFMA and EF24 With the 26S Proteasome
    Article Snippet: Highly purified human 26, 20, and 19S proteasomes were obtained from Boston Biochem (Cambridge, MA). .. Anti-actin primary antibody, horse radish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody, and HRP-streptavidin were from Sigma-Aldrich (St. Louis, MO).

    SDS Page:

    Article Title: 4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils
    Article Snippet: Paragraph title: 4.6. SDS-PAGE and Western Blotting ... The blotted membrane was blocked in 2% Block Ace Solution (Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan) for 1 h, probed with the mouse anti-GAPDH mAb (16 ng/mL) or rabbit anticathepsin G pAb (0.5 μ g/mL) for 2 h, and then visualized using the biotinylated antimouse or antirabbit immunoglobulins pAb, HRP-streptavidin, and Immobilon Western Chemiluminescent HRP Substrate (Millipore Co.).

    Article Title: Listeria monocytogenes Triggers the Cell Surface Expression of Gp96 Protein and Interacts with Its N Terminus to Support Cellular Infection *
    Article Snippet: Samples were analyzed by SDS-PAGE followed by immunoblotting. .. Primary and secondary antibodies were diluted in 2.5% skim milk in buffer A as follows: anti-Gp96, 1:2000; anti-E-cadherin, 1:500; anti-β-catenin (H-102, sc-7199, Santa Cruz Biotechnology), 1:500; anti-actin (AC-15, Sigma-Aldrich), 1:5000; HRP-conjugated secondary antibodies (PARIS Anticorps), 1:2000; and HRP-streptavidin (Sigma-Aldrich), 1:2000.

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: Paragraph title: SDS-page and immunoblotting ... To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152).

    Software:

    Article Title: 4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils
    Article Snippet: The blotted membrane was blocked in 2% Block Ace Solution (Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan) for 1 h, probed with the mouse anti-GAPDH mAb (16 ng/mL) or rabbit anticathepsin G pAb (0.5 μ g/mL) for 2 h, and then visualized using the biotinylated antimouse or antirabbit immunoglobulins pAb, HRP-streptavidin, and Immobilon Western Chemiluminescent HRP Substrate (Millipore Co.). .. The density of each band was quantified by Multi Gauge software (Version 3.0; Fujifilm Co.) and expressed in arbitrary units.

    Electrophoresis:

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: Electrophoresis of unmodified and biotinylated BDNF were carried out using 12% SDS-page gels. .. To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152).

    Article Title: Heligmosomoides polygyrus elicits a dominant non-protective antibody response directed against restricted glycan and peptide epitopes
    Article Snippet: Paragraph title: 1D and 2D gel electrophoresis and Western blotting ... Alternatively, IgA blots were incubated with biotinylated anti-mouse IgA (1/500, Invitrogen ) followed by HRP-streptavidin (1/2000, Sigma), and developed as above.

    Negative Control:

    Article Title: Shortened Engineered Human Antibody CH2 Domains
    Article Snippet: Briefly, CH2, m01, m01s, and human serum albumin (negative control) were coated on 96-well plates at a concentration of 2 μg/ml. .. HRP-streptavidin (Sigma) was used for detection of biotinylated mouse anti-human CH2 antibody.

    Binding Assay:

    Article Title: Genome-Wide Analysis of PDZ Domain Binding Reveals Inherent Functional Overlap within the PDZ Interaction Network
    Article Snippet: .. For the binding assay, biotinylated peptides (4 µM) (purchased from United Peptide Corporation, UPC) were used, which were detected with HRP-Streptavidin and HRP substrate 3,3,5,5-Tetramethylbenzidine (TMB) (Sigma). ..

    Preserving:

    Article Title: Enhancement of soluble CD28 levels in the serum of Graves’ disease
    Article Snippet: After washing twice, soluble CD28 captured by coating the antibody was incubated with biotin-conjugated anti-CD28 mAb (prepared in our lab, clone 2D5), followed by HRP-streptavidin (Sigma, MO, USA) incubation for 1 hour at 37°C and then reacted with the substrate TMB (KPL, UK). .. In addition, stability was analysed by the detection with four known soluble CD28 concentrations (1 ng/ml, 5 ng/ml, 10 ng/ml and 15 ng/ml) after plate coating and preservation at 4°C for 60 days.

    Concentration Assay:

    Article Title: Enhancement of soluble CD28 levels in the serum of Graves’ disease
    Article Snippet: Detection of soluble CD28 by ELISA The concentration of sCD28 in plasma was evaluated by ELISA. .. After washing twice, soluble CD28 captured by coating the antibody was incubated with biotin-conjugated anti-CD28 mAb (prepared in our lab, clone 2D5), followed by HRP-streptavidin (Sigma, MO, USA) incubation for 1 hour at 37°C and then reacted with the substrate TMB (KPL, UK).

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: To check the concentration of proteins and verify their molecular weights, SDS gels were stained with 0.025% Coomassie Blue R solution (Bio-Rad) for 1 hr before washing in destaining solutions of 7% acetic acid and 40% methanol. .. To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152).

    Article Title: Shortened Engineered Human Antibody CH2 Domains
    Article Snippet: Briefly, CH2, m01, m01s, and human serum albumin (negative control) were coated on 96-well plates at a concentration of 2 μg/ml. .. HRP-streptavidin (Sigma) was used for detection of biotinylated mouse anti-human CH2 antibody.

    Article Title: A Novel Method for Producing Mono-biotinylated, Biologically Active Neurotrophic Factors: An Essential Reagent for Single Molecule Study of Axonal Transport
    Article Snippet: HRP-streptavidin was from Chemicon (San Diego, CA, Cat# 18-152). .. For precipitation of proteins with trichloroacetic acid (TCA from Sigma, Cat# T6399), 100% ice-cold TCA was added to the supernatant to a final concentration of 5-7% and incubated on ice for 20 min.

    Two-Dimensional Gel Electrophoresis:

    Article Title: Heligmosomoides polygyrus elicits a dominant non-protective antibody response directed against restricted glycan and peptide epitopes
    Article Snippet: Paragraph title: 1D and 2D gel electrophoresis and Western blotting ... Alternatively, IgA blots were incubated with biotinylated anti-mouse IgA (1/500, Invitrogen ) followed by HRP-streptavidin (1/2000, Sigma), and developed as above.

    Staining:

    Article Title: 4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils
    Article Snippet: For silver staining, 12% polyacrylamide gels were stained using a Wako Silver Stain II Kit (Wako Pure Chemical Industries Ltd.). .. The blotted membrane was blocked in 2% Block Ace Solution (Dainippon Sumitomo Pharma Co. Ltd., Osaka, Japan) for 1 h, probed with the mouse anti-GAPDH mAb (16 ng/mL) or rabbit anticathepsin G pAb (0.5 μ g/mL) for 2 h, and then visualized using the biotinylated antimouse or antirabbit immunoglobulins pAb, HRP-streptavidin, and Immobilon Western Chemiluminescent HRP Substrate (Millipore Co.).

    Article Title: Tubulovesicular Structures within Vesicular Stomatitis Virus G Protein-Pseudotyped Lentiviral Vector Preparations Carry DNA and Stimulate Antiviral Responses via Toll-Like Receptor 9 ▿
    Article Snippet: After blocking in 5% bovine serum albumin, the membrane was stained with mouse anti-VSV-G (MAb P5D4 ( ) (Cancer Research UK), mouse anti-GFP (MAb GFP 3E1; Cancer Research UK), mouse anti-β-actin (MAb clone AC-15; Sigma), or biotinylated mouse anti-HIVp24 (Aalto). .. Secondary reagents were horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Santa Cruz Biotechnology) or HRP-streptavidin (Sigma).

    Article Title: Functional characterization and axonal transport of quantum dot labeled BDNF
    Article Snippet: To check the concentration of proteins and verify their molecular weights, SDS gels were stained with 0.025% Coomassie Blue R solution (Bio-Rad) for 1 hr before washing in destaining solutions of 7% acetic acid and 40% methanol. .. To verify the addition of biotin labels to BDNF, proteins separated by SDS gel were transferred onto a PVDF membrane (Fisher Scientific), blocked in 5% milk, and incubated with HRP-streptavidin (Millipore, Billerica, MA; Cat# 18-152).

    Article Title: Heligmosomoides polygyrus elicits a dominant non-protective antibody response directed against restricted glycan and peptide epitopes
    Article Snippet: HES and HEx (1-10 μg) were separated, silver stained or blotted as previously described ( ). .. Alternatively, IgA blots were incubated with biotinylated anti-mouse IgA (1/500, Invitrogen ) followed by HRP-streptavidin (1/2000, Sigma), and developed as above.

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  • 95
    Millipore hrp conjugated streptavidin
    Discovery and validation of SLRP–Hsp47 interactions. A , far-Western blotting to detect potential intracellular SLRP interactants. Lysates of chondrocyte-like ATDC5 cells were run on SDS-PAGE, and the proteins were transferred onto a nitrocellulose membrane. Membrane strips were blocked with BSA and incubated with biotinylated proteins: BSA (negative control), decorin (DCN), fibromodulin (FM), or lumican (LUM). Binding was detected with <t>streptavidin-HRP</t> and peroxidase substrate. The arrow marks a presumed common interactant of DCN, FM, and LUM. The corresponding region was cut out from the SDS-polyacrylamide gel and analyzed by MS. B , co-immunoprecipitation assays to detect potential SLRP–Hsp47 interactions. Lysates from ATDC5 cells were used alone (−) or mixed with 1 μg of recombinant DCN, His-tagged FM, or His-tagged LUM. The lysates were incubated with decorin rabbit anti-serum ( IP : DCN ) or mouse anti-His antibodies ( IP : his-tag ) and with Protein A– or Protein G–Sepharose, respectively. Co-precipitated proteins were run on SDS-PAGE and immunoblotted for Hsp47 ( WB : HSP47 ). The capture of SLRPs was validated with the corresponding antisera ( WB : DCN , FM , LUM ). C , pulldown assays to validate SLRP–Hsp47 interactions. Magnetic beads were coated with BSA or Hsp47 (bait) and incubated with biotinylated DCN, FM, or LUM (all prey). The beads were washed, and the eluates were run on SDS-PAGE. The binding was detected by blotting with streptavidin-HRP.
    Hrp Conjugated Streptavidin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated streptavidin/product/Millipore
    Average 95 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated streptavidin - by Bioz Stars, 2020-02
    95/100 stars
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    95
    Millipore streptavidin conjugated to hrp
    Discovery and validation of SLRP–Hsp47 interactions. A , far-Western blotting to detect potential intracellular SLRP interactants. Lysates of chondrocyte-like ATDC5 cells were run on SDS-PAGE, and the proteins were transferred onto a nitrocellulose membrane. Membrane strips were blocked with BSA and incubated with biotinylated proteins: BSA (negative control), decorin (DCN), fibromodulin (FM), or lumican (LUM). Binding was detected with <t>streptavidin-HRP</t> and peroxidase substrate. The arrow marks a presumed common interactant of DCN, FM, and LUM. The corresponding region was cut out from the SDS-polyacrylamide gel and analyzed by MS. B , co-immunoprecipitation assays to detect potential SLRP–Hsp47 interactions. Lysates from ATDC5 cells were used alone (−) or mixed with 1 μg of recombinant DCN, His-tagged FM, or His-tagged LUM. The lysates were incubated with decorin rabbit anti-serum ( IP : DCN ) or mouse anti-His antibodies ( IP : his-tag ) and with Protein A– or Protein G–Sepharose, respectively. Co-precipitated proteins were run on SDS-PAGE and immunoblotted for Hsp47 ( WB : HSP47 ). The capture of SLRPs was validated with the corresponding antisera ( WB : DCN , FM , LUM ). C , pulldown assays to validate SLRP–Hsp47 interactions. Magnetic beads were coated with BSA or Hsp47 (bait) and incubated with biotinylated DCN, FM, or LUM (all prey). The beads were washed, and the eluates were run on SDS-PAGE. The binding was detected by blotting with streptavidin-HRP.
    Streptavidin Conjugated To Hrp, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Discovery and validation of SLRP–Hsp47 interactions. A , far-Western blotting to detect potential intracellular SLRP interactants. Lysates of chondrocyte-like ATDC5 cells were run on SDS-PAGE, and the proteins were transferred onto a nitrocellulose membrane. Membrane strips were blocked with BSA and incubated with biotinylated proteins: BSA (negative control), decorin (DCN), fibromodulin (FM), or lumican (LUM). Binding was detected with streptavidin-HRP and peroxidase substrate. The arrow marks a presumed common interactant of DCN, FM, and LUM. The corresponding region was cut out from the SDS-polyacrylamide gel and analyzed by MS. B , co-immunoprecipitation assays to detect potential SLRP–Hsp47 interactions. Lysates from ATDC5 cells were used alone (−) or mixed with 1 μg of recombinant DCN, His-tagged FM, or His-tagged LUM. The lysates were incubated with decorin rabbit anti-serum ( IP : DCN ) or mouse anti-His antibodies ( IP : his-tag ) and with Protein A– or Protein G–Sepharose, respectively. Co-precipitated proteins were run on SDS-PAGE and immunoblotted for Hsp47 ( WB : HSP47 ). The capture of SLRPs was validated with the corresponding antisera ( WB : DCN , FM , LUM ). C , pulldown assays to validate SLRP–Hsp47 interactions. Magnetic beads were coated with BSA or Hsp47 (bait) and incubated with biotinylated DCN, FM, or LUM (all prey). The beads were washed, and the eluates were run on SDS-PAGE. The binding was detected by blotting with streptavidin-HRP.

    Journal: The Journal of Biological Chemistry

    Article Title: The endoplasmic reticulum–resident collagen chaperone Hsp47 interacts with and promotes the secretion of decorin, fibromodulin, and lumican

    doi: 10.1074/jbc.RA117.000758

    Figure Lengend Snippet: Discovery and validation of SLRP–Hsp47 interactions. A , far-Western blotting to detect potential intracellular SLRP interactants. Lysates of chondrocyte-like ATDC5 cells were run on SDS-PAGE, and the proteins were transferred onto a nitrocellulose membrane. Membrane strips were blocked with BSA and incubated with biotinylated proteins: BSA (negative control), decorin (DCN), fibromodulin (FM), or lumican (LUM). Binding was detected with streptavidin-HRP and peroxidase substrate. The arrow marks a presumed common interactant of DCN, FM, and LUM. The corresponding region was cut out from the SDS-polyacrylamide gel and analyzed by MS. B , co-immunoprecipitation assays to detect potential SLRP–Hsp47 interactions. Lysates from ATDC5 cells were used alone (−) or mixed with 1 μg of recombinant DCN, His-tagged FM, or His-tagged LUM. The lysates were incubated with decorin rabbit anti-serum ( IP : DCN ) or mouse anti-His antibodies ( IP : his-tag ) and with Protein A– or Protein G–Sepharose, respectively. Co-precipitated proteins were run on SDS-PAGE and immunoblotted for Hsp47 ( WB : HSP47 ). The capture of SLRPs was validated with the corresponding antisera ( WB : DCN , FM , LUM ). C , pulldown assays to validate SLRP–Hsp47 interactions. Magnetic beads were coated with BSA or Hsp47 (bait) and incubated with biotinylated DCN, FM, or LUM (all prey). The beads were washed, and the eluates were run on SDS-PAGE. The binding was detected by blotting with streptavidin-HRP.

    Article Snippet: Then the membrane was incubated with HRP-conjugated streptavidin diluted 1:50,000 in blocking buffer and incubated for 1 h, washed 3 × 10 min in TBST, and developed using Luminata forte (Millipore) and a CCD camera.

    Techniques: Far Western Blot, SDS Page, Incubation, Negative Control, Binding Assay, Mass Spectrometry, Immunoprecipitation, Recombinant, Western Blot, Magnetic Beads