streptavidin poly  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin poly
    Streptavidin Poly, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin poly/product/Thermo Fisher
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin poly - by Bioz Stars, 2020-03
    83/100 stars

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    Western Blot:

    Article Title: Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers
    Article Snippet: Each eluted protein sample was split into two; one portion was used for Western detection and the other was subjected to in-gel digestion followed by mass spectrometric analysis. .. The membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 and 1% bovine serum albumin (BSA), then hybridized with streptavidin–poly(horseradish peroxidase) conjugate (SA-HRP; Pierce Protein Biology Products, Rockford, IL, USA) in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Corp., Osaka, Japan) for two hours.

    Sample Prep:

    Article Title: Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers
    Article Snippet: Paragraph title: Detection of crosslinked products, and sample preparation for protein identification ... The membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 and 1% bovine serum albumin (BSA), then hybridized with streptavidin–poly(horseradish peroxidase) conjugate (SA-HRP; Pierce Protein Biology Products, Rockford, IL, USA) in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Corp., Osaka, Japan) for two hours.

    Molecular Weight:

    Article Title: Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers
    Article Snippet: The membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 and 1% bovine serum albumin (BSA), then hybridized with streptavidin–poly(horseradish peroxidase) conjugate (SA-HRP; Pierce Protein Biology Products, Rockford, IL, USA) in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Corp., Osaka, Japan) for two hours. .. The gel was stained with SYPRO Ruby protein gel stain (Molecular Probes, Eugene, OR), and the gel regions with the corresponding molecular weight to the chemiluminescent bands observed on SA-HRP detection were excised and sliced into 1 mm3 pieces.

    Staining:

    Article Title: Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers
    Article Snippet: The membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 and 1% bovine serum albumin (BSA), then hybridized with streptavidin–poly(horseradish peroxidase) conjugate (SA-HRP; Pierce Protein Biology Products, Rockford, IL, USA) in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Corp., Osaka, Japan) for two hours. .. The gel was stained with SYPRO Ruby protein gel stain (Molecular Probes, Eugene, OR), and the gel regions with the corresponding molecular weight to the chemiluminescent bands observed on SA-HRP detection were excised and sliced into 1 mm3 pieces.

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    Thermo Fisher streptavidin poly hrp
    Schematic of an amplification strategy using <t>streptavidin</t> <t>poly-HRP.</t> The streptavidin poly-HRP is attached to the biotinylated anti-human IL-6 detection antibody.
    Streptavidin Poly Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin poly hrp/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    streptavidin poly hrp - by Bioz Stars, 2020-03
    99/100 stars
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    Schematic of an amplification strategy using streptavidin poly-HRP. The streptavidin poly-HRP is attached to the biotinylated anti-human IL-6 detection antibody.

    Journal: Lab on a Chip

    Article Title: Fabrication of immunosensor microwell arrays from gold compact discs for detection of cancer biomarker proteins †

    doi: 10.1039/c1lc20833k

    Figure Lengend Snippet: Schematic of an amplification strategy using streptavidin poly-HRP. The streptavidin poly-HRP is attached to the biotinylated anti-human IL-6 detection antibody.

    Article Snippet: In this work, we chose an alternative, rapid amplification strategy that substitutes streptavidin poly-HRP for the more commonly used streptavidin HRP to improve sensitivity and limit of detection.

    Techniques: Amplification

    H4 10 Bpa cross-linking patterns of the mutant Mediator complexes. SDS-PAGE blot probed with streptavidin poly-HRP to detect and compare the pattern of biotinylated peptide cross-linked to Mediator subunits in WT Mediator and the mutant Mediator complexes. Equimolar amounts (∼7.5 nM) of each indicated Mediator complex were incubated with H4 10 Bpa (4 µM) and exposed to UV for 15 min. Asterisk refers to the same weak signal as in Fig. 2-A.

    Journal: PLoS ONE

    Article Title: Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions

    doi: 10.1371/journal.pone.0038416

    Figure Lengend Snippet: H4 10 Bpa cross-linking patterns of the mutant Mediator complexes. SDS-PAGE blot probed with streptavidin poly-HRP to detect and compare the pattern of biotinylated peptide cross-linked to Mediator subunits in WT Mediator and the mutant Mediator complexes. Equimolar amounts (∼7.5 nM) of each indicated Mediator complex were incubated with H4 10 Bpa (4 µM) and exposed to UV for 15 min. Asterisk refers to the same weak signal as in Fig. 2-A.

    Article Snippet: SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits resolved on 10% SDS-polyacrylamide.

    Techniques: Mutagenesis, SDS Page, Incubation

    H4 10 Bpa has four specific cross-linking targets within Mediator. (A) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits. WT H4, H4 10 Bpa and H4 22 Bpa (4 µM) were incubated in the presence or absence of the WT Mediator complex (∼7.5 nM) and exposed to UV for 15 min when indicated. Cross-linking products were resolved on 6% SDS-polyacrylamide gel, transferred to to PVDF, detected by streptavidin poly-HRP, and referred to as BCTs (H4 10 B pa C ross-linking T argets). A weak band with relatively poor reproducibility was asterisked. (B) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits after different UV exposure times. Identical mixtures, which contain 7.5 nM Mediator complex and 4 µM H4 10 Bpa, were exposed to UV for 0, 5, 10 or 15 min. (C) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits in reactions with varient H4 10 Bpa concentration. WT Mediator complex (∼7.5 nM) was incubated with 1 µM, 2 µM or 4 µM H4 10 Bpa peptide and exposed to UV irradiation for 15 min. (D) Western blot analysis of histone tail peptide binding experiment comparing Mediator binding affinity for H4 10 Bpa and H2B tail peptide under the identical concentrations to the cross-linking reactions. WT Mediator complex (∼3 nM) was mixed with H4 10 Bpa (4 µM) or synthetic biotinylated histone H2B N’-tail peptide (4 µM or 8 µM) as the inputs. The basic steps and layout of the analysis were as described earlier ( Fig. 1 ). (E) and (F) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits, after H4 22 Bpa (E) or H2B tail peptide (F) was added at the indicated concentration.

    Journal: PLoS ONE

    Article Title: Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions

    doi: 10.1371/journal.pone.0038416

    Figure Lengend Snippet: H4 10 Bpa has four specific cross-linking targets within Mediator. (A) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits. WT H4, H4 10 Bpa and H4 22 Bpa (4 µM) were incubated in the presence or absence of the WT Mediator complex (∼7.5 nM) and exposed to UV for 15 min when indicated. Cross-linking products were resolved on 6% SDS-polyacrylamide gel, transferred to to PVDF, detected by streptavidin poly-HRP, and referred to as BCTs (H4 10 B pa C ross-linking T argets). A weak band with relatively poor reproducibility was asterisked. (B) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits after different UV exposure times. Identical mixtures, which contain 7.5 nM Mediator complex and 4 µM H4 10 Bpa, were exposed to UV for 0, 5, 10 or 15 min. (C) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits in reactions with varient H4 10 Bpa concentration. WT Mediator complex (∼7.5 nM) was incubated with 1 µM, 2 µM or 4 µM H4 10 Bpa peptide and exposed to UV irradiation for 15 min. (D) Western blot analysis of histone tail peptide binding experiment comparing Mediator binding affinity for H4 10 Bpa and H2B tail peptide under the identical concentrations to the cross-linking reactions. WT Mediator complex (∼3 nM) was mixed with H4 10 Bpa (4 µM) or synthetic biotinylated histone H2B N’-tail peptide (4 µM or 8 µM) as the inputs. The basic steps and layout of the analysis were as described earlier ( Fig. 1 ). (E) and (F) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits, after H4 22 Bpa (E) or H2B tail peptide (F) was added at the indicated concentration.

    Article Snippet: SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits resolved on 10% SDS-polyacrylamide.

    Techniques: SDS Page, Incubation, Concentration Assay, Irradiation, Western Blot, Binding Assay

    Med5(Nut1)p, Med14(Rgr1)p, Med17(Srb4)p and Med1p are H4 10 Bpa cross-linking targets. Silver staining (A) and immunoblotting analysis (B) comparing the composition of affinity-purified MYC -tagged and non- MYC -tagged WT Mediator complexes after 10% SDS-PAGE. (C) A comparison of the cross-linking patterns of MYC -tagged Mediator complexes with the WT pattern using an SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits. Each indicated Mediator species (∼7.5 nM) was incubated with H4 10 Bpa (4 µM). A ‘Long Exposure’ of the 79 kD region on the SDS-PAGE blot probed with streptavidin poly-HRP is shown for a better view of the weak BCT4 signal in each sample.

    Journal: PLoS ONE

    Article Title: Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions

    doi: 10.1371/journal.pone.0038416

    Figure Lengend Snippet: Med5(Nut1)p, Med14(Rgr1)p, Med17(Srb4)p and Med1p are H4 10 Bpa cross-linking targets. Silver staining (A) and immunoblotting analysis (B) comparing the composition of affinity-purified MYC -tagged and non- MYC -tagged WT Mediator complexes after 10% SDS-PAGE. (C) A comparison of the cross-linking patterns of MYC -tagged Mediator complexes with the WT pattern using an SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits. Each indicated Mediator species (∼7.5 nM) was incubated with H4 10 Bpa (4 µM). A ‘Long Exposure’ of the 79 kD region on the SDS-PAGE blot probed with streptavidin poly-HRP is shown for a better view of the weak BCT4 signal in each sample.

    Article Snippet: SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits resolved on 10% SDS-polyacrylamide.

    Techniques: Silver Staining, Affinity Purification, SDS Page, Incubation