streptavidin peroxidase complex  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin peroxidase complex
    Apoptotic activity in the left ventricle. A. showing high expression of caspase- 3 in_cardiac myocytes (arrow head) surrounding the necrotic area, endothelial cells (thin arrow), and neutrophil polymorphs (thick arrow) in 24-hour post MI, Alexa Fluor 488 immunofluorescent technique. B. showing no expression of caspase 3 in 24-hour sham-operated left ventricle. C, showing high expression of cleaved caspase- 3 in cardiac myocytes (thick arrows). Many apoptotic bodies (arrow head) are seen in the infarcted area of left ventricle, <t>streptavidin-</t> biotin immunoperoxidase method. D, showing very low apoptotic activity, only one cell stained with anti-cleaved caspase 3 (arrow head) in the area of infraction in the left ventricle of 4-hour post MI, streptavidin- biotin immunoperoxidase method. E, F, Showing no staining with anti-cleaved caspase 3 in the left ventricle of 30 minutes and 60 minutes post MI, streptavidin- biotin immunoperoxidase method.
    Streptavidin Peroxidase Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 33 article reviews
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    streptavidin peroxidase complex - by Bioz Stars, 2020-07
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    Images

    1) Product Images from "Galectin-1 in Early Acute Myocardial Infarction"

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0086994

    Apoptotic activity in the left ventricle. A. showing high expression of caspase- 3 in_cardiac myocytes (arrow head) surrounding the necrotic area, endothelial cells (thin arrow), and neutrophil polymorphs (thick arrow) in 24-hour post MI, Alexa Fluor 488 immunofluorescent technique. B. showing no expression of caspase 3 in 24-hour sham-operated left ventricle. C, showing high expression of cleaved caspase- 3 in cardiac myocytes (thick arrows). Many apoptotic bodies (arrow head) are seen in the infarcted area of left ventricle, streptavidin- biotin immunoperoxidase method. D, showing very low apoptotic activity, only one cell stained with anti-cleaved caspase 3 (arrow head) in the area of infraction in the left ventricle of 4-hour post MI, streptavidin- biotin immunoperoxidase method. E, F, Showing no staining with anti-cleaved caspase 3 in the left ventricle of 30 minutes and 60 minutes post MI, streptavidin- biotin immunoperoxidase method.
    Figure Legend Snippet: Apoptotic activity in the left ventricle. A. showing high expression of caspase- 3 in_cardiac myocytes (arrow head) surrounding the necrotic area, endothelial cells (thin arrow), and neutrophil polymorphs (thick arrow) in 24-hour post MI, Alexa Fluor 488 immunofluorescent technique. B. showing no expression of caspase 3 in 24-hour sham-operated left ventricle. C, showing high expression of cleaved caspase- 3 in cardiac myocytes (thick arrows). Many apoptotic bodies (arrow head) are seen in the infarcted area of left ventricle, streptavidin- biotin immunoperoxidase method. D, showing very low apoptotic activity, only one cell stained with anti-cleaved caspase 3 (arrow head) in the area of infraction in the left ventricle of 4-hour post MI, streptavidin- biotin immunoperoxidase method. E, F, Showing no staining with anti-cleaved caspase 3 in the left ventricle of 30 minutes and 60 minutes post MI, streptavidin- biotin immunoperoxidase method.

    Techniques Used: Activity Assay, Expressing, Staining

    Galectin-1 expression 30 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. A galectin-1 positive control section from prostate gland showing cytoplasmic expression of galectin- 1 by prostatic acini. E, F G. Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. H, I J. Representative sections from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.
    Figure Legend Snippet: Galectin-1 expression 30 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. A galectin-1 positive control section from prostate gland showing cytoplasmic expression of galectin- 1 by prostatic acini. E, F G. Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. H, I J. Representative sections from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.

    Techniques Used: Expressing, Ligation, Negative Control, Staining, Positive Control

    Ki-67 proliferative activity in the left ventricle. A, B, C, D, Low Ki-67 proliferative activity in normal left ventricle showing nuclear staining of Ki-67 in one interstitial cell (arrow head). E, F, G, 20 minutes MI; I,J, K, 30 minutes MI; M,N,O 60 minutes MI; Q,R,S, 4-hour MI, Low Ki-67 proliferative activity: showing nuclear staining of Ki-67 in few endothelial cells (arrow head) in left ventricle. U, V, W, 24-hour MI, high Ki-67 proliferative activity showing nuclear staining of Ki-67 in a large number of endothelial cells (arrow head) in the infarction area of left ventricle. H, L, P, T, X, showing faint nuclear staining of Ki-67 in one endothelial cell in 20 minutes, 30 minutes, 60 minutes, 4-hours and 24 hour sham-operated left ventricle. A, E, I, M, Q, U are stained by Streptavidin-Biotin immunoperoxidase method. The others are stained by Alexa Fluor 488 immunofluorescent technique.
    Figure Legend Snippet: Ki-67 proliferative activity in the left ventricle. A, B, C, D, Low Ki-67 proliferative activity in normal left ventricle showing nuclear staining of Ki-67 in one interstitial cell (arrow head). E, F, G, 20 minutes MI; I,J, K, 30 minutes MI; M,N,O 60 minutes MI; Q,R,S, 4-hour MI, Low Ki-67 proliferative activity: showing nuclear staining of Ki-67 in few endothelial cells (arrow head) in left ventricle. U, V, W, 24-hour MI, high Ki-67 proliferative activity showing nuclear staining of Ki-67 in a large number of endothelial cells (arrow head) in the infarction area of left ventricle. H, L, P, T, X, showing faint nuclear staining of Ki-67 in one endothelial cell in 20 minutes, 30 minutes, 60 minutes, 4-hours and 24 hour sham-operated left ventricle. A, E, I, M, Q, U are stained by Streptavidin-Biotin immunoperoxidase method. The others are stained by Alexa Fluor 488 immunofluorescent technique.

    Techniques Used: Activity Assay, Staining

    Galectin-1 expression 4 hours following ligation of LAD. A.A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing low expression of galectin-1 in the left ventricle (thin arrow) and right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), streptavidin- biotin immunoperoxidase method. E F, Representative section of the left ventricle from area supplied by LAD showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), Alexa Fluor 488 immunofluorescent technique.
    Figure Legend Snippet: Galectin-1 expression 4 hours following ligation of LAD. A.A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing low expression of galectin-1 in the left ventricle (thin arrow) and right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), streptavidin- biotin immunoperoxidase method. E F, Representative section of the left ventricle from area supplied by LAD showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), Alexa Fluor 488 immunofluorescent technique.

    Techniques Used: Expressing, Ligation, Negative Control, Staining

    HIF-1 α expression of the heart. A. Representative section of naïve heart showing nuclear expression of HIF-1 α by few endothelial cells (arrow head), streptavidin- biotin immunoperoxidase method. B. Negative control section showing no HIF-1 α staining, streptavidin- biotin immunoperoxidase method. C. Positive control section of mouse placenta showing nuclear staining of HIF-1 α by trophoblastic cells, Rhodamine, immunofluorescent technique. D. Negative control section for HIF-1 α. Rhodamine, immunofluorescent technique. E,I,M Q shows representative sections from the anterior wall of left ventricle in the area supplied by LAD 20 min, 30 min, 60 min and 4 hours following ligation of LAD, showing variable nuclear staining of HIF-1 α by cardiac myocytes at different time points (arrow head) and endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. F,J,N R shows their corresponding Sham operated hearts showing low nuclear expression of HIF-1 α by few endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. Low and high power views of the left ventricle 20 min (G H), 30 min (K L), 60 min (O P) and 4 hours (S T) following ligation of LAD showing high nuclear staining of HIF-1 α by cardiac myocytes (arrow head), Rhodamine, immunofluorescent technique.
    Figure Legend Snippet: HIF-1 α expression of the heart. A. Representative section of naïve heart showing nuclear expression of HIF-1 α by few endothelial cells (arrow head), streptavidin- biotin immunoperoxidase method. B. Negative control section showing no HIF-1 α staining, streptavidin- biotin immunoperoxidase method. C. Positive control section of mouse placenta showing nuclear staining of HIF-1 α by trophoblastic cells, Rhodamine, immunofluorescent technique. D. Negative control section for HIF-1 α. Rhodamine, immunofluorescent technique. E,I,M Q shows representative sections from the anterior wall of left ventricle in the area supplied by LAD 20 min, 30 min, 60 min and 4 hours following ligation of LAD, showing variable nuclear staining of HIF-1 α by cardiac myocytes at different time points (arrow head) and endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. F,J,N R shows their corresponding Sham operated hearts showing low nuclear expression of HIF-1 α by few endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. Low and high power views of the left ventricle 20 min (G H), 30 min (K L), 60 min (O P) and 4 hours (S T) following ligation of LAD showing high nuclear staining of HIF-1 α by cardiac myocytes (arrow head), Rhodamine, immunofluorescent technique.

    Techniques Used: Expressing, Negative Control, Staining, Positive Control, Ligation

    Galectin-1 and HIF-1 α expression 24 hours following ligation of LAD. A. A high power view of left ventricle in an area supplied by LAD showing coagulative necrosis (thin arrows) accompanied by heavy neutrophil polymorphs infiltration (arrow heads), H E. B. A low power view of the heart showing areas of high expression of galectin-1 (thick arrows) surrounding areas of no expression (star shape) in the left ventricle and interventricular septum in the area supplied by LAD, streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thick arrows), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow), surrounding areas of no expression (thin arrow). Many neutrophil polymorphs are also showing cytoplasmic expression of Galectin-1(arrow head), Streptavidin- biotin immunoperoxidase method. H, I J. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow) surrounding areas of no expression (thin arrow), many neutrophil polymorphs (arrow head) show cytoplasmic expression of Galectin-1, Alexa Fluor 488 immunofluorescent technique. K. Representative section from left ventricle showing nuclear expression of HIF-1 α by cardiac myocytes (arrow head) and neutrophil polymorphs (thin arrow), Rhodamine, immunofluorescent technique. L. Representative section from left ventricle showing cytoplasmic expression of Galectin-1 by cardiac myocytes (thick arrow), endothelial cells (arrow head) and neutrophil polymorphs (thin arrows), Alexa Fluor 488 immunofluorescent technique. M. Co-localization of Galectin-1 and HIF-1 α in cardiac myocytes (thick arrow), endothelial cell (arrow head) and neutrophil polymorphs (thin arrow), Alexa Fluor-Rhodamine immunofluorescent technique.
    Figure Legend Snippet: Galectin-1 and HIF-1 α expression 24 hours following ligation of LAD. A. A high power view of left ventricle in an area supplied by LAD showing coagulative necrosis (thin arrows) accompanied by heavy neutrophil polymorphs infiltration (arrow heads), H E. B. A low power view of the heart showing areas of high expression of galectin-1 (thick arrows) surrounding areas of no expression (star shape) in the left ventricle and interventricular septum in the area supplied by LAD, streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thick arrows), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow), surrounding areas of no expression (thin arrow). Many neutrophil polymorphs are also showing cytoplasmic expression of Galectin-1(arrow head), Streptavidin- biotin immunoperoxidase method. H, I J. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow) surrounding areas of no expression (thin arrow), many neutrophil polymorphs (arrow head) show cytoplasmic expression of Galectin-1, Alexa Fluor 488 immunofluorescent technique. K. Representative section from left ventricle showing nuclear expression of HIF-1 α by cardiac myocytes (arrow head) and neutrophil polymorphs (thin arrow), Rhodamine, immunofluorescent technique. L. Representative section from left ventricle showing cytoplasmic expression of Galectin-1 by cardiac myocytes (thick arrow), endothelial cells (arrow head) and neutrophil polymorphs (thin arrows), Alexa Fluor 488 immunofluorescent technique. M. Co-localization of Galectin-1 and HIF-1 α in cardiac myocytes (thick arrow), endothelial cell (arrow head) and neutrophil polymorphs (thin arrow), Alexa Fluor-Rhodamine immunofluorescent technique.

    Techniques Used: Expressing, Ligation, Negative Control, Staining

    Bcl2 activity in left ventricle. A, B, C, D, showing high cytoplasmic expression of bcl2 by cardiac myocytes (thick arrow), and endothelial cells (arrow head) at 30 minutes post MI in an area supplied by LAD in the left ventricle, streptavidin- biotin immunoperoxidase method. E, F, showing very low expression of bcl2 in few endothelial cells (arrow head) in the left ventricle of naïve and 30-minutes sham operated heart. Streptavidin- biotin immunoperoxidase method.
    Figure Legend Snippet: Bcl2 activity in left ventricle. A, B, C, D, showing high cytoplasmic expression of bcl2 by cardiac myocytes (thick arrow), and endothelial cells (arrow head) at 30 minutes post MI in an area supplied by LAD in the left ventricle, streptavidin- biotin immunoperoxidase method. E, F, showing very low expression of bcl2 in few endothelial cells (arrow head) in the left ventricle of naïve and 30-minutes sham operated heart. Streptavidin- biotin immunoperoxidase method.

    Techniques Used: Activity Assay, Expressing

    Galectin-1 expression 60 minutes following ligation of LAD. A B, A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing low expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G, Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or absence of expression (star shape), streptavidin- biotin immunoperoxidase method. H, I J, Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.
    Figure Legend Snippet: Galectin-1 expression 60 minutes following ligation of LAD. A B, A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing low expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G, Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or absence of expression (star shape), streptavidin- biotin immunoperoxidase method. H, I J, Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.

    Techniques Used: Expressing, Ligation, Negative Control, Staining

    Galectin-1 expression 20 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of galectin-1 staining. D, E F. Representative sections of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow D). High power views show a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) and endothelial cells (arrow head, E F), There is also nuclear expression of galectin 1 by cardiac myocytes (thick arrow E F),streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle shows a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow).There is also high expression of galectin-1 in the Z bands (arrow head), Alexa Fluor 488 immunofluorescent technique.
    Figure Legend Snippet: Galectin-1 expression 20 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of galectin-1 staining. D, E F. Representative sections of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow D). High power views show a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) and endothelial cells (arrow head, E F), There is also nuclear expression of galectin 1 by cardiac myocytes (thick arrow E F),streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle shows a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow).There is also high expression of galectin-1 in the Z bands (arrow head), Alexa Fluor 488 immunofluorescent technique.

    Techniques Used: Expressing, Ligation, Negative Control, Staining

    2) Product Images from "Dysregulated expression of microRNAs and mRNAs in myocardial infarction"

    Article Title: Dysregulated expression of microRNAs and mRNAs in myocardial infarction

    Journal: American Journal of Translational Research

    doi:

    HIF1α expression of the heart of MI mice with treatment of antagomiRs. A B. The expression of HIF1α in the nuclei of cardiomyocytes was detected in three-day MI mice heart with treatment of antagomiR-210. C D. Four weeks of MI when treatment with antagomiR-210 relative to antagomiR_MM group. Streptavidin- biotin immunoperoxidase method was used.
    Figure Legend Snippet: HIF1α expression of the heart of MI mice with treatment of antagomiRs. A B. The expression of HIF1α in the nuclei of cardiomyocytes was detected in three-day MI mice heart with treatment of antagomiR-210. C D. Four weeks of MI when treatment with antagomiR-210 relative to antagomiR_MM group. Streptavidin- biotin immunoperoxidase method was used.

    Techniques Used: Expressing, Mouse Assay

    3) Product Images from "Galectin-1: a biomarker of surgical stress in murine model of cardiac surgery"

    Article Title: Galectin-1: a biomarker of surgical stress in murine model of cardiac surgery

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Galectin-1 in the heart. A. Low power view of heart from non-operated control group showing low and patchy expression of GAL-1, mainly in the right ventricle (arrow head) and interventricular septum (thin arrow), Immunoperoxidase streptavidin-biotin method.
    Figure Legend Snippet: Galectin-1 in the heart. A. Low power view of heart from non-operated control group showing low and patchy expression of GAL-1, mainly in the right ventricle (arrow head) and interventricular septum (thin arrow), Immunoperoxidase streptavidin-biotin method.

    Techniques Used: Expressing

    Related Articles

    Flow Cytometry:

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    In Vitro:

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    Isolation:

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    Cytometry:

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    Infection:

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    Mouse Assay:

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    Incubation:

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    Labeling:

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    Article Snippet: .. In vitro binding and internalization assays Antibodies, Fab fragments, streptavidin (Invitrogen), and bovine serum albumin (BSA, Fisher Scientific) were covalently labeled with Alexa Fluor 488 sulfodichlorophenol ester (Alexa488) and Alexa Fluor 750 succinimidyl esters (Alexa750) (Life Technologies). ..

    Article Title: The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4
    Article Snippet: .. Isolated leukocytes from lungs of uninfected control as well as infected mice were stained with labeled monoclonal antibodies (mAbs) specific for cell surface markers or intracellular transcription factors or cytokines. mAbs for flow cytometry specific for CD11c (-Alexa-fluor 700), CD11b (-PECy7 or -APCCy7), MHCII (-PE), F4/80 (-PerCP-Cy5.5), DCsign (-APC), CD8(-APCCy7 -PECy5), CD4 (- FITC), Ly6c (-efluor-450), CD80 (-PE), CD86 (-PECy5), CD115 (-FITC), CD3 (-FITC) and CD3 (-efluor-450) were obtained from eBioscience and CD68 (-biotinylated plus streptavidin conjugated with Qdot605) was obteined from Invitrogen. .. The intracellular fixation and permeabilization buffer set of eBioscience was used to perform IFN-γ- APC, TNF-α-PE and NOS2-PE.

    Protease Inhibitor:

    Article Title: TRMP, a p53-inducible long noncoding RNA, regulates G1/S cell cycle progression by modulating IRES-dependent p27 translation
    Article Snippet: .. Reagents and antibodies The following reagents used in this study were purchased from the indicated sources: cycloheximide (Sigma, 20 μg/ml), doxorubicin (Sigma, 0.5 μg/ml), doxycycline (Sigma, 1 μg/ml), Hoechst 33342 (Sigma, 1 μg/ml), lipofectamine 2000 (Invitrogen), streptavidin-coated agarose beads (Thermo Fisher Scientific), complete EDTA-free protease inhibitor cocktail (Roche Applied Science), antibodies against GAPDH (Santa Cruz, sc-166545, 1:5000), p53 (Santa Cruz, sc-126, 1:1000), p21 (Sigma, #P1484, 1:2000), Flag (Sigma, #F3165, 1:4000), p27 (Abcam, Y236, 1:1000), cyclin D1 (Santa Cruz, sc-753, 1:1000), cyclin E1 (Santa Cruz, sc-481, 1:1000), PTBP1 (Proteintech, 12582-1-AP, 1:1000), HRP-conjugated secondary antibodies against mouse (115-035-062), and rabbit (111-035-144) (Jackson ImmunoResearch, 1:10000). .. Cell culture A549 and H1299 cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma) supplemented with 10% FBS and antibiotics (GIBCO).

    Staining:

    Article Title: The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4
    Article Snippet: .. Isolated leukocytes from lungs of uninfected control as well as infected mice were stained with labeled monoclonal antibodies (mAbs) specific for cell surface markers or intracellular transcription factors or cytokines. mAbs for flow cytometry specific for CD11c (-Alexa-fluor 700), CD11b (-PECy7 or -APCCy7), MHCII (-PE), F4/80 (-PerCP-Cy5.5), DCsign (-APC), CD8(-APCCy7 -PECy5), CD4 (- FITC), Ly6c (-efluor-450), CD80 (-PE), CD86 (-PECy5), CD115 (-FITC), CD3 (-FITC) and CD3 (-efluor-450) were obtained from eBioscience and CD68 (-biotinylated plus streptavidin conjugated with Qdot605) was obteined from Invitrogen. .. The intracellular fixation and permeabilization buffer set of eBioscience was used to perform IFN-γ- APC, TNF-α-PE and NOS2-PE.

    Binding Assay:

    Article Title: Side-by-Side Comparison of Commonly Used Biomolecules That Differ in Size and Affinity on Tumor Uptake and Internalization
    Article Snippet: .. In vitro binding and internalization assays Antibodies, Fab fragments, streptavidin (Invitrogen), and bovine serum albumin (BSA, Fisher Scientific) were covalently labeled with Alexa Fluor 488 sulfodichlorophenol ester (Alexa488) and Alexa Fluor 750 succinimidyl esters (Alexa750) (Life Technologies). ..

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  • 88
    Thermo Fisher streptavidin horse radish peroxidase hrp
    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a <t>streptavidin-horse</t> radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.
    Streptavidin Horse Radish Peroxidase Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horse radish peroxidase hrp/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    streptavidin horse radish peroxidase hrp - by Bioz Stars, 2020-07
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    92
    Thermo Fisher streptavidin peroxidase complex
    Apoptotic activity in the left ventricle. A. showing high expression of caspase- 3 in_cardiac myocytes (arrow head) surrounding the necrotic area, endothelial cells (thin arrow), and neutrophil polymorphs (thick arrow) in 24-hour post MI, Alexa Fluor 488 immunofluorescent technique. B. showing no expression of caspase 3 in 24-hour sham-operated left ventricle. C, showing high expression of cleaved caspase- 3 in cardiac myocytes (thick arrows). Many apoptotic bodies (arrow head) are seen in the infarcted area of left ventricle, <t>streptavidin-</t> biotin immunoperoxidase method. D, showing very low apoptotic activity, only one cell stained with anti-cleaved caspase 3 (arrow head) in the area of infraction in the left ventricle of 4-hour post MI, streptavidin- biotin immunoperoxidase method. E, F, Showing no staining with anti-cleaved caspase 3 in the left ventricle of 30 minutes and 60 minutes post MI, streptavidin- biotin immunoperoxidase method.
    Streptavidin Peroxidase Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin peroxidase complex/product/Thermo Fisher
    Average 92 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    streptavidin peroxidase complex - by Bioz Stars, 2020-07
    92/100 stars
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    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a streptavidin-horse radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.

    Journal: PLoS ONE

    Article Title: p53 Dimers Associate with a Head-to-Tail Response Element to Repress Cyclin B Transcription

    doi: 10.1371/journal.pone.0042615

    Figure Lengend Snippet: Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a streptavidin-horse radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.

    Article Snippet: The electrophoretic mobility of the complexes was detected using streptavidin-horse radish peroxidase (HRP) and a chemiluminescent substrate (Thermo Scientific).

    Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Incubation, Recombinant, Labeling, Polyacrylamide Gel Electrophoresis

    Apoptotic activity in the left ventricle. A. showing high expression of caspase- 3 in_cardiac myocytes (arrow head) surrounding the necrotic area, endothelial cells (thin arrow), and neutrophil polymorphs (thick arrow) in 24-hour post MI, Alexa Fluor 488 immunofluorescent technique. B. showing no expression of caspase 3 in 24-hour sham-operated left ventricle. C, showing high expression of cleaved caspase- 3 in cardiac myocytes (thick arrows). Many apoptotic bodies (arrow head) are seen in the infarcted area of left ventricle, streptavidin- biotin immunoperoxidase method. D, showing very low apoptotic activity, only one cell stained with anti-cleaved caspase 3 (arrow head) in the area of infraction in the left ventricle of 4-hour post MI, streptavidin- biotin immunoperoxidase method. E, F, Showing no staining with anti-cleaved caspase 3 in the left ventricle of 30 minutes and 60 minutes post MI, streptavidin- biotin immunoperoxidase method.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Apoptotic activity in the left ventricle. A. showing high expression of caspase- 3 in_cardiac myocytes (arrow head) surrounding the necrotic area, endothelial cells (thin arrow), and neutrophil polymorphs (thick arrow) in 24-hour post MI, Alexa Fluor 488 immunofluorescent technique. B. showing no expression of caspase 3 in 24-hour sham-operated left ventricle. C, showing high expression of cleaved caspase- 3 in cardiac myocytes (thick arrows). Many apoptotic bodies (arrow head) are seen in the infarcted area of left ventricle, streptavidin- biotin immunoperoxidase method. D, showing very low apoptotic activity, only one cell stained with anti-cleaved caspase 3 (arrow head) in the area of infraction in the left ventricle of 4-hour post MI, streptavidin- biotin immunoperoxidase method. E, F, Showing no staining with anti-cleaved caspase 3 in the left ventricle of 30 minutes and 60 minutes post MI, streptavidin- biotin immunoperoxidase method.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Activity Assay, Expressing, Staining

    Galectin-1 expression 30 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. A galectin-1 positive control section from prostate gland showing cytoplasmic expression of galectin- 1 by prostatic acini. E, F G. Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. H, I J. Representative sections from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Galectin-1 expression 30 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. A galectin-1 positive control section from prostate gland showing cytoplasmic expression of galectin- 1 by prostatic acini. E, F G. Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. H, I J. Representative sections from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing, Ligation, Negative Control, Staining, Positive Control

    Ki-67 proliferative activity in the left ventricle. A, B, C, D, Low Ki-67 proliferative activity in normal left ventricle showing nuclear staining of Ki-67 in one interstitial cell (arrow head). E, F, G, 20 minutes MI; I,J, K, 30 minutes MI; M,N,O 60 minutes MI; Q,R,S, 4-hour MI, Low Ki-67 proliferative activity: showing nuclear staining of Ki-67 in few endothelial cells (arrow head) in left ventricle. U, V, W, 24-hour MI, high Ki-67 proliferative activity showing nuclear staining of Ki-67 in a large number of endothelial cells (arrow head) in the infarction area of left ventricle. H, L, P, T, X, showing faint nuclear staining of Ki-67 in one endothelial cell in 20 minutes, 30 minutes, 60 minutes, 4-hours and 24 hour sham-operated left ventricle. A, E, I, M, Q, U are stained by Streptavidin-Biotin immunoperoxidase method. The others are stained by Alexa Fluor 488 immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Ki-67 proliferative activity in the left ventricle. A, B, C, D, Low Ki-67 proliferative activity in normal left ventricle showing nuclear staining of Ki-67 in one interstitial cell (arrow head). E, F, G, 20 minutes MI; I,J, K, 30 minutes MI; M,N,O 60 minutes MI; Q,R,S, 4-hour MI, Low Ki-67 proliferative activity: showing nuclear staining of Ki-67 in few endothelial cells (arrow head) in left ventricle. U, V, W, 24-hour MI, high Ki-67 proliferative activity showing nuclear staining of Ki-67 in a large number of endothelial cells (arrow head) in the infarction area of left ventricle. H, L, P, T, X, showing faint nuclear staining of Ki-67 in one endothelial cell in 20 minutes, 30 minutes, 60 minutes, 4-hours and 24 hour sham-operated left ventricle. A, E, I, M, Q, U are stained by Streptavidin-Biotin immunoperoxidase method. The others are stained by Alexa Fluor 488 immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Activity Assay, Staining

    Galectin-1 expression 4 hours following ligation of LAD. A.A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing low expression of galectin-1 in the left ventricle (thin arrow) and right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), streptavidin- biotin immunoperoxidase method. E F, Representative section of the left ventricle from area supplied by LAD showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), Alexa Fluor 488 immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Galectin-1 expression 4 hours following ligation of LAD. A.A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing low expression of galectin-1 in the left ventricle (thin arrow) and right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of staining of galectin-1. D. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), streptavidin- biotin immunoperoxidase method. E F, Representative section of the left ventricle from area supplied by LAD showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or no expression (star shape), Alexa Fluor 488 immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing, Ligation, Negative Control, Staining

    HIF-1 α expression of the heart. A. Representative section of naïve heart showing nuclear expression of HIF-1 α by few endothelial cells (arrow head), streptavidin- biotin immunoperoxidase method. B. Negative control section showing no HIF-1 α staining, streptavidin- biotin immunoperoxidase method. C. Positive control section of mouse placenta showing nuclear staining of HIF-1 α by trophoblastic cells, Rhodamine, immunofluorescent technique. D. Negative control section for HIF-1 α. Rhodamine, immunofluorescent technique. E,I,M Q shows representative sections from the anterior wall of left ventricle in the area supplied by LAD 20 min, 30 min, 60 min and 4 hours following ligation of LAD, showing variable nuclear staining of HIF-1 α by cardiac myocytes at different time points (arrow head) and endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. F,J,N R shows their corresponding Sham operated hearts showing low nuclear expression of HIF-1 α by few endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. Low and high power views of the left ventricle 20 min (G H), 30 min (K L), 60 min (O P) and 4 hours (S T) following ligation of LAD showing high nuclear staining of HIF-1 α by cardiac myocytes (arrow head), Rhodamine, immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: HIF-1 α expression of the heart. A. Representative section of naïve heart showing nuclear expression of HIF-1 α by few endothelial cells (arrow head), streptavidin- biotin immunoperoxidase method. B. Negative control section showing no HIF-1 α staining, streptavidin- biotin immunoperoxidase method. C. Positive control section of mouse placenta showing nuclear staining of HIF-1 α by trophoblastic cells, Rhodamine, immunofluorescent technique. D. Negative control section for HIF-1 α. Rhodamine, immunofluorescent technique. E,I,M Q shows representative sections from the anterior wall of left ventricle in the area supplied by LAD 20 min, 30 min, 60 min and 4 hours following ligation of LAD, showing variable nuclear staining of HIF-1 α by cardiac myocytes at different time points (arrow head) and endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. F,J,N R shows their corresponding Sham operated hearts showing low nuclear expression of HIF-1 α by few endothelial cells (thin arrow), streptavidin- biotin immunoperoxidase method. Low and high power views of the left ventricle 20 min (G H), 30 min (K L), 60 min (O P) and 4 hours (S T) following ligation of LAD showing high nuclear staining of HIF-1 α by cardiac myocytes (arrow head), Rhodamine, immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing, Negative Control, Staining, Positive Control, Ligation

    Galectin-1 and HIF-1 α expression 24 hours following ligation of LAD. A. A high power view of left ventricle in an area supplied by LAD showing coagulative necrosis (thin arrows) accompanied by heavy neutrophil polymorphs infiltration (arrow heads), H E. B. A low power view of the heart showing areas of high expression of galectin-1 (thick arrows) surrounding areas of no expression (star shape) in the left ventricle and interventricular septum in the area supplied by LAD, streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thick arrows), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow), surrounding areas of no expression (thin arrow). Many neutrophil polymorphs are also showing cytoplasmic expression of Galectin-1(arrow head), Streptavidin- biotin immunoperoxidase method. H, I J. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow) surrounding areas of no expression (thin arrow), many neutrophil polymorphs (arrow head) show cytoplasmic expression of Galectin-1, Alexa Fluor 488 immunofluorescent technique. K. Representative section from left ventricle showing nuclear expression of HIF-1 α by cardiac myocytes (arrow head) and neutrophil polymorphs (thin arrow), Rhodamine, immunofluorescent technique. L. Representative section from left ventricle showing cytoplasmic expression of Galectin-1 by cardiac myocytes (thick arrow), endothelial cells (arrow head) and neutrophil polymorphs (thin arrows), Alexa Fluor 488 immunofluorescent technique. M. Co-localization of Galectin-1 and HIF-1 α in cardiac myocytes (thick arrow), endothelial cell (arrow head) and neutrophil polymorphs (thin arrow), Alexa Fluor-Rhodamine immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Galectin-1 and HIF-1 α expression 24 hours following ligation of LAD. A. A high power view of left ventricle in an area supplied by LAD showing coagulative necrosis (thin arrows) accompanied by heavy neutrophil polymorphs infiltration (arrow heads), H E. B. A low power view of the heart showing areas of high expression of galectin-1 (thick arrows) surrounding areas of no expression (star shape) in the left ventricle and interventricular septum in the area supplied by LAD, streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thick arrows), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G. Representative section of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow), surrounding areas of no expression (thin arrow). Many neutrophil polymorphs are also showing cytoplasmic expression of Galectin-1(arrow head), Streptavidin- biotin immunoperoxidase method. H, I J. Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thick arrow) surrounding areas of no expression (thin arrow), many neutrophil polymorphs (arrow head) show cytoplasmic expression of Galectin-1, Alexa Fluor 488 immunofluorescent technique. K. Representative section from left ventricle showing nuclear expression of HIF-1 α by cardiac myocytes (arrow head) and neutrophil polymorphs (thin arrow), Rhodamine, immunofluorescent technique. L. Representative section from left ventricle showing cytoplasmic expression of Galectin-1 by cardiac myocytes (thick arrow), endothelial cells (arrow head) and neutrophil polymorphs (thin arrows), Alexa Fluor 488 immunofluorescent technique. M. Co-localization of Galectin-1 and HIF-1 α in cardiac myocytes (thick arrow), endothelial cell (arrow head) and neutrophil polymorphs (thin arrow), Alexa Fluor-Rhodamine immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing, Ligation, Negative Control, Staining

    Bcl2 activity in left ventricle. A, B, C, D, showing high cytoplasmic expression of bcl2 by cardiac myocytes (thick arrow), and endothelial cells (arrow head) at 30 minutes post MI in an area supplied by LAD in the left ventricle, streptavidin- biotin immunoperoxidase method. E, F, showing very low expression of bcl2 in few endothelial cells (arrow head) in the left ventricle of naïve and 30-minutes sham operated heart. Streptavidin- biotin immunoperoxidase method.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Bcl2 activity in left ventricle. A, B, C, D, showing high cytoplasmic expression of bcl2 by cardiac myocytes (thick arrow), and endothelial cells (arrow head) at 30 minutes post MI in an area supplied by LAD in the left ventricle, streptavidin- biotin immunoperoxidase method. E, F, showing very low expression of bcl2 in few endothelial cells (arrow head) in the left ventricle of naïve and 30-minutes sham operated heart. Streptavidin- biotin immunoperoxidase method.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Activity Assay, Expressing

    Galectin-1 expression 60 minutes following ligation of LAD. A B, A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing low expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G, Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or absence of expression (star shape), streptavidin- biotin immunoperoxidase method. H, I J, Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Galectin-1 expression 60 minutes following ligation of LAD. A B, A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. C. Sham operated heart showing low expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. D. Negative control section of the heart showing absence of staining of galectin-1. E, F G, Representative sections of the left ventricle from areas supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) surrounding areas of low or absence of expression (star shape), streptavidin- biotin immunoperoxidase method. H, I J, Representative section from left ventricle showing a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow), Alexa Fluor 488 immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing, Ligation, Negative Control, Staining

    Galectin-1 expression 20 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of galectin-1 staining. D, E F. Representative sections of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow D). High power views show a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) and endothelial cells (arrow head, E F), There is also nuclear expression of galectin 1 by cardiac myocytes (thick arrow E F),streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle shows a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow).There is also high expression of galectin-1 in the Z bands (arrow head), Alexa Fluor 488 immunofluorescent technique.

    Journal: PLoS ONE

    Article Title: Galectin-1 in Early Acute Myocardial Infarction

    doi: 10.1371/journal.pone.0086994

    Figure Lengend Snippet: Galectin-1 expression 20 minutes following ligation of LAD. A. A low power view of the heart showing a high expression of galectin-1 in the anterior wall of left ventricle in the area supplied by LAD (thin arrow) and the interventricular septum (thick arrow). There is also increase in the expression of galectin-1 in the right ventricle (arrow head), streptavidin- biotin immunoperoxidase method. B. Sham operated heart showing lower expression of galectin-1 in the left ventricle and right ventricle (thin arrow), streptavidin- biotin immunoperoxidase method. C. Negative control section of the heart showing absence of galectin-1 staining. D, E F. Representative sections of the left ventricle from area supplied by LAD showing high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow D). High power views show a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow) and endothelial cells (arrow head, E F), There is also nuclear expression of galectin 1 by cardiac myocytes (thick arrow E F),streptavidin- biotin immunoperoxidase method. G, H I. Representative section from left ventricle shows a well demarcated area of high cytoplasmic expression of galectin-1 by cardiac myocytes (thin arrow).There is also high expression of galectin-1 in the Z bands (arrow head), Alexa Fluor 488 immunofluorescent technique.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing, Ligation, Negative Control, Staining

    HIF1α expression of the heart of MI mice with treatment of antagomiRs. A B. The expression of HIF1α in the nuclei of cardiomyocytes was detected in three-day MI mice heart with treatment of antagomiR-210. C D. Four weeks of MI when treatment with antagomiR-210 relative to antagomiR_MM group. Streptavidin- biotin immunoperoxidase method was used.

    Journal: American Journal of Translational Research

    Article Title: Dysregulated expression of microRNAs and mRNAs in myocardial infarction

    doi:

    Figure Lengend Snippet: HIF1α expression of the heart of MI mice with treatment of antagomiRs. A B. The expression of HIF1α in the nuclei of cardiomyocytes was detected in three-day MI mice heart with treatment of antagomiR-210. C D. Four weeks of MI when treatment with antagomiR-210 relative to antagomiR_MM group. Streptavidin- biotin immunoperoxidase method was used.

    Article Snippet: Finally, sections were incubated with streptavidin–peroxidase complex for 20 minutes at room temperature (Thermo Scientific), DAB chromogen (Thermo Scientific) added and counter staining done with haematoxylin.

    Techniques: Expressing, Mouse Assay

    Galectin-1 in the heart. A. Low power view of heart from non-operated control group showing low and patchy expression of GAL-1, mainly in the right ventricle (arrow head) and interventricular septum (thin arrow), Immunoperoxidase streptavidin-biotin method.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Galectin-1: a biomarker of surgical stress in murine model of cardiac surgery

    doi:

    Figure Lengend Snippet: Galectin-1 in the heart. A. Low power view of heart from non-operated control group showing low and patchy expression of GAL-1, mainly in the right ventricle (arrow head) and interventricular septum (thin arrow), Immunoperoxidase streptavidin-biotin method.

    Article Snippet: Finally, sections were incubated with streptavidin-peroxidase complex for 20 minutes at room temperature (Thermo Scientific, USA), DAB chromogen (Thermo Scientific, USA) added and counter staining done with haematoxylin.

    Techniques: Expressing