streptavidin magnetic beads  (New England Biolabs)


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    Name:
    Streptavidin Magnetic Beads
    Description:
    Streptavidin Magnetic Beads 5 ml
    Catalog Number:
    s1420s
    Price:
    303
    Size:
    5 ml
    Category:
    Magnetic Separation Equipment
    Buy from Supplier


    Structured Review

    New England Biolabs streptavidin magnetic beads
    Streptavidin Magnetic Beads
    Streptavidin Magnetic Beads 5 ml
    https://www.bioz.com/result/streptavidin magnetic beads/product/New England Biolabs
    Average 99 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    streptavidin magnetic beads - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: 60-mm dishes of HEK cells were transfected 1:3 (GFP: gene of interest) with either hERG-NT or -CM. .. Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol.

    Magnetic Beads:

    Article Title: Nascent chromatin occupancy profiling reveals locus- and factor-specific chromatin maturation dynamics behind the DNA replication fork
    Article Snippet: .. Biotin conjugated EdU-labeled DNA was enriched using 5 µL streptavidin magnetic beads (New England Biolabs). .. Beads were resuspended in blocking solution (2% I-Block [Thermo Fisher Scientific], 25 mM Tris, 150 mM NaCl, and 0.5% SDS) for 2 h with gentle shaking at room temperature.

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol. .. Isolated RNA was then cleaned up using Zymo RNA Clean and Concentrator-5 kit (catalogue no. R1015, Zymo).

    Article Title: Synthetic HIV V3 Glycopeptide Immunogen Carrying a N334 N-Glycan Induces Glycan-Dependent Antibodies with Promiscuous Site Recognition
    Article Snippet: .. Streptavidin magnetic beads (1 mg, New England BioLabs) were first incubated with the synthetic A244 V3 peptide 10 (0.5 mg/mL, 500 μ L) at 37 °C for 30 min. After washing the beads three times with PBS buffer, 1 mL of antisera elicited by glycopeptide immunogen 15 was added and incubated for 30 min at 37 °C to subtract the peptide specific antibodies. ..

    Isolation:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol. .. Isolated RNA was then cleaned up using Zymo RNA Clean and Concentrator-5 kit (catalogue no. R1015, Zymo).

    Labeling:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: 24 h post-transfection, cells were exposed to 4-thiouridine (catalogue no. T4509-25MG, Sigma) for 30 min. Total RNA was then harvested, and RNA was isolated using RNeasy mini kit (catalogue no. 74104, Qiagen); RNA was then labeled with EZ-Link HPDP-Biotin (catalogue no. 21341, ThermoFisher Scientific), and RNA was purified with Zymo RNA Clean and Concentrator-25 (catalogue no. R1017, Zymo, Irvine, CA). .. Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol.

    Purification:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: 24 h post-transfection, cells were exposed to 4-thiouridine (catalogue no. T4509-25MG, Sigma) for 30 min. Total RNA was then harvested, and RNA was isolated using RNeasy mini kit (catalogue no. 74104, Qiagen); RNA was then labeled with EZ-Link HPDP-Biotin (catalogue no. 21341, ThermoFisher Scientific), and RNA was purified with Zymo RNA Clean and Concentrator-25 (catalogue no. R1017, Zymo, Irvine, CA). .. Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol.

    Real-time Polymerase Chain Reaction:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol. .. 1 μg of total RNA was then converted to cDNA using the SuperScript VILO cDNA synthesis kit (catalogue no. 11754050, ThermoFisher Scientific), and the qPCR protocol as described above was followed.

    Incubation:

    Article Title: Nascent chromatin occupancy profiling reveals locus- and factor-specific chromatin maturation dynamics behind the DNA replication fork
    Article Snippet: DNA was incubated in click chemistry reaction buffer as previously described ( ; ; ), with the exception that CuSO4 and ascorbic acid were replaced with CuBr and TBTA (Sigma-Aldrich). .. Biotin conjugated EdU-labeled DNA was enriched using 5 µL streptavidin magnetic beads (New England Biolabs).

    Article Title: Synthetic HIV V3 Glycopeptide Immunogen Carrying a N334 N-Glycan Induces Glycan-Dependent Antibodies with Promiscuous Site Recognition
    Article Snippet: .. Streptavidin magnetic beads (1 mg, New England BioLabs) were first incubated with the synthetic A244 V3 peptide 10 (0.5 mg/mL, 500 μ L) at 37 °C for 30 min. After washing the beads three times with PBS buffer, 1 mL of antisera elicited by glycopeptide immunogen 15 was added and incubated for 30 min at 37 °C to subtract the peptide specific antibodies. ..

    Blocking Assay:

    Article Title: Nascent chromatin occupancy profiling reveals locus- and factor-specific chromatin maturation dynamics behind the DNA replication fork
    Article Snippet: Biotin conjugated EdU-labeled DNA was enriched using 5 µL streptavidin magnetic beads (New England Biolabs). .. Beads were resuspended in blocking solution (2% I-Block [Thermo Fisher Scientific], 25 mM Tris, 150 mM NaCl, and 0.5% SDS) for 2 h with gentle shaking at room temperature.

    Modification:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: To determine the rate of nascent mRNA hERG-NT and hERG-CM production, the protocol from Newman et al. ( ) was modified. .. Biotin-labeled RNA was then isolated with streptavidin magnetic beads (catalogue no. S1420, New England Biolabs) and eluted in 5% 2-mercaptoethanol.

    Binding Assay:

    Article Title: Nascent chromatin occupancy profiling reveals locus- and factor-specific chromatin maturation dynamics behind the DNA replication fork
    Article Snippet: Biotin conjugated EdU-labeled DNA was enriched using 5 µL streptavidin magnetic beads (New England Biolabs). .. Beads were then washed twice with cold binding buffer ( ).

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  • 90
    New England Biolabs streptavidin magnetic beads
    Streptavidin Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin magnetic beads/product/New England Biolabs
    Average 90 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    streptavidin magnetic beads - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    New England Biolabs hydrophilic streptavidin magnetic beads
    SMRT-Cappable-seq identifies full-length transcripts in bacteria. a Schema of the SMRT-Cappable-seq methodology. 5′ triphosphorylated transcripts are capped with a desthio-biotinylated (DTB) cap analog and bound to the <t>streptavidin</t> beads to specifically capture primary transcripts starting at TSS. The polyadenylation step (A-tailing) ensures the priming of the anchored poly dT primer for cDNA synthesis at the most 3′end of the transcript. b Integrative Genomics Viewer (IGV) representation of the mapping of SMRT-Cappable-seq reads (top) compared to Illumina RNA-seq reads (bottom) in the mprA locus. Forward oriented reads are labeled in pink, reverse oriented reads are labeled in blue. c Comparison between gene expression level in Read counts Per Kilobase of transcript, per Million mapped reads (RPKM) for Illumina RNA-seq and SMRT-Cappable-seq. The Spearman’s rank correlation is 0.798 ( p value
    Hydrophilic Streptavidin Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydrophilic streptavidin magnetic beads/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydrophilic streptavidin magnetic beads - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results


    SMRT-Cappable-seq identifies full-length transcripts in bacteria. a Schema of the SMRT-Cappable-seq methodology. 5′ triphosphorylated transcripts are capped with a desthio-biotinylated (DTB) cap analog and bound to the streptavidin beads to specifically capture primary transcripts starting at TSS. The polyadenylation step (A-tailing) ensures the priming of the anchored poly dT primer for cDNA synthesis at the most 3′end of the transcript. b Integrative Genomics Viewer (IGV) representation of the mapping of SMRT-Cappable-seq reads (top) compared to Illumina RNA-seq reads (bottom) in the mprA locus. Forward oriented reads are labeled in pink, reverse oriented reads are labeled in blue. c Comparison between gene expression level in Read counts Per Kilobase of transcript, per Million mapped reads (RPKM) for Illumina RNA-seq and SMRT-Cappable-seq. The Spearman’s rank correlation is 0.798 ( p value

    Journal: Nature Communications

    Article Title: SMRT-Cappable-seq reveals complex operon variants in bacteria

    doi: 10.1038/s41467-018-05997-6

    Figure Lengend Snippet: SMRT-Cappable-seq identifies full-length transcripts in bacteria. a Schema of the SMRT-Cappable-seq methodology. 5′ triphosphorylated transcripts are capped with a desthio-biotinylated (DTB) cap analog and bound to the streptavidin beads to specifically capture primary transcripts starting at TSS. The polyadenylation step (A-tailing) ensures the priming of the anchored poly dT primer for cDNA synthesis at the most 3′end of the transcript. b Integrative Genomics Viewer (IGV) representation of the mapping of SMRT-Cappable-seq reads (top) compared to Illumina RNA-seq reads (bottom) in the mprA locus. Forward oriented reads are labeled in pink, reverse oriented reads are labeled in blue. c Comparison between gene expression level in Read counts Per Kilobase of transcript, per Million mapped reads (RPKM) for Illumina RNA-seq and SMRT-Cappable-seq. The Spearman’s rank correlation is 0.798 ( p value

    Article Snippet: The capped RNA was enriched using hydrophilic streptavidin magnetic beads (New England Biolabs).

    Techniques: RNA Sequencing Assay, Labeling, Expressing