streptavidin hrp complex  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin hrp complex
    Streptavidin Hrp Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin hrp complex/product/Thermo Fisher
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin hrp complex - by Bioz Stars, 2020-03
    80/100 stars

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    Related Articles

    Microscopy:

    Article Title: Matrix Metalloprotease 8-Dependent Extracellular Matrix Cleavage at the Blood–CSF Barrier Contributes to Lethality during Systemic Inflammatory Diseases
    Article Snippet: After another washing step, sections were counterstained with Hoechst, mounted, and visualized with a Leica TCS SP5 II confocal microscope. .. Sections were again rinsed in PBS and incubated with streptavidin–HRP complex (85-9043; Zymed) for 45 min. After rinsing in PBS, sections were incubated in 3,30-diaminobenzidine (Merck) and H2 O2 /PBS for 6 min.

    Immunohistochemistry:

    Article Title: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, the tissue sections were treated with biotinylated anti-mouse secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin HRP complex (Zymed).

    Labeling:

    Article Title: Matrix Metalloprotease 8-Dependent Extracellular Matrix Cleavage at the Blood–CSF Barrier Contributes to Lethality during Systemic Inflammatory Diseases
    Article Snippet: For immunoperoxidase labeling of glial fibrillary acidic protein (GFAP) (Z0334; Dako) and CD45 (550539; BD PharMingen), sections were incubated in H2 O2 /PBS for 10 min. After rinsing in PBS, sections were boiled in citrate buffer and again rinsed. .. Sections were again rinsed in PBS and incubated with streptavidin–HRP complex (85-9043; Zymed) for 45 min. After rinsing in PBS, sections were incubated in 3,30-diaminobenzidine (Merck) and H2 O2 /PBS for 6 min.

    Incubation:

    Article Title: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-mouse secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin HRP complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored by two independent observers.

    Article Title: Matrix Metalloprotease 8-Dependent Extracellular Matrix Cleavage at the Blood–CSF Barrier Contributes to Lethality during Systemic Inflammatory Diseases
    Article Snippet: .. Sections were again rinsed in PBS and incubated with streptavidin–HRP complex (85-9043; Zymed) for 45 min. After rinsing in PBS, sections were incubated in 3,30-diaminobenzidine (Merck) and H2 O2 /PBS for 6 min. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-mouse secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin HRP complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored by two independent observers.

    Immunostaining:

    Article Title: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-mouse secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin HRP complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored by two independent observers.

    Expressing:

    Article Title: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
    Article Snippet: Immunohistochemistry Immunohistochemistry was done to study altered protein expression in 171 human ESCC tissue,which was the same samples as our previous report[ ]. .. After washing, the tissue sections were treated with biotinylated anti-mouse secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin HRP complex (Zymed).

    Blocking Assay:

    Article Title: Matrix Metalloprotease 8-Dependent Extracellular Matrix Cleavage at the Blood–CSF Barrier Contributes to Lethality during Systemic Inflammatory Diseases
    Article Snippet: Sections were then incubated with blocking buffer (PBS supplemented with 10% FCS and 1% Triton X-100) for 15 min, followed by overnight incubation with primary antibody: GFAP (1:200) or CD45 (1:100). .. Sections were again rinsed in PBS and incubated with streptavidin–HRP complex (85-9043; Zymed) for 45 min. After rinsing in PBS, sections were incubated in 3,30-diaminobenzidine (Merck) and H2 O2 /PBS for 6 min.

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    Thermo Fisher streptavidin horse radish peroxidase hrp
    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a <t>streptavidin-horse</t> radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.
    Streptavidin Horse Radish Peroxidase Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horse radish peroxidase hrp/product/Thermo Fisher
    Average 80 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    streptavidin horse radish peroxidase hrp - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    99
    Thermo Fisher avidin hrp
    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a <t>streptavidin-horse</t> radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.
    Avidin Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin hrp/product/Thermo Fisher
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    avidin hrp - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a streptavidin-horse radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.

    Journal: PLoS ONE

    Article Title: p53 Dimers Associate with a Head-to-Tail Response Element to Repress Cyclin B Transcription

    doi: 10.1371/journal.pone.0042615

    Figure Lengend Snippet: Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a streptavidin-horse radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.

    Article Snippet: The electrophoretic mobility of the complexes was detected using streptavidin-horse radish peroxidase (HRP) and a chemiluminescent substrate (Thermo Scientific).

    Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Incubation, Recombinant, Labeling, Polyacrylamide Gel Electrophoresis

    Identification of genome-wide association of Klf9 in HT22 cell chromatin using chromatin-streptavidin precipitation sequencing. a HT22 [BirA/FLBIO-Klf9] cells express biotinylated Klf9. Whole cell extracts from the HT22 parent cell line (lane 1), HT22 [BirA] cells (lane 2) or HT22 [BirA/FLBIO-Klf9] (lane 3) were fractionated on 10% SDS-PAGE and analyzed by Western blotting using streptavidin-HRP. b Chromatin-streptavidin precipitation gives ~25-fold enrichment at the Klf13 promoter in HT22 [BirA/FLBIO-Klf9] cells compared with HT22 [BirA] cells. Precipitated DNA was analyzed by qPCR at the Klf13 promoter and intron (negative control region). The asterisk indicates a statistically significant difference by Student’s two-sample t -test ( p

    Journal: BMC Genomics

    Article Title: The Krüppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding

    doi: 10.1186/s12864-017-3640-7

    Figure Lengend Snippet: Identification of genome-wide association of Klf9 in HT22 cell chromatin using chromatin-streptavidin precipitation sequencing. a HT22 [BirA/FLBIO-Klf9] cells express biotinylated Klf9. Whole cell extracts from the HT22 parent cell line (lane 1), HT22 [BirA] cells (lane 2) or HT22 [BirA/FLBIO-Klf9] (lane 3) were fractionated on 10% SDS-PAGE and analyzed by Western blotting using streptavidin-HRP. b Chromatin-streptavidin precipitation gives ~25-fold enrichment at the Klf13 promoter in HT22 [BirA/FLBIO-Klf9] cells compared with HT22 [BirA] cells. Precipitated DNA was analyzed by qPCR at the Klf13 promoter and intron (negative control region). The asterisk indicates a statistically significant difference by Student’s two-sample t -test ( p

    Article Snippet: To detect FLBIO-Klf9 we incubated the membrane with Streptavidin-HRP (Thermo; diluted 1:1000) for 1 h. Immune or streptavidin-HRP complexes were revealed by chemiluminescence detection using Pierce ECL Substrate (ThermoFisher Scientific).

    Techniques: GWAS, Sequencing, SDS Page, Western Blot, Real-time Polymerase Chain Reaction, Negative Control