streptavidin horseradish peroxidase reporter probe  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin horseradish peroxidase reporter probe
    Streptavidin Horseradish Peroxidase Reporter Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horseradish peroxidase reporter probe/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin horseradish peroxidase reporter probe - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Protein Binding:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: After any remaining protein binding sites were blocked with 1% normal mouse serum in Tris-buffered saline supplemented with 0.05% Tween-20 (TTBS, pH 7.4), wells were washed four times in TTBS, then human CSF at 1% or varying concentrations of recombinant human S100β standards (Genway #288258) prepared in normal mouse serum in TTBS supplemented with 1 mM CaCl2 were added for 90 minutes at 22°C (100 µl per well). .. After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C.

    Incubation:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: .. After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C. ..

    Purification:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: Each assay was normalized using serial 2-fold dilutions of standard proteins: brain extract treated with activated calpain and a purified bovine spinal cord neurofilament preparation, respectively. .. After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C.

    Sandwich ELISA:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: For the S100β sandwich ELISA, the S100β capture antibody (Abcam AB8334 at 1/10,000 dilution) was adsorbed onto Nunc maxisorp 96 well plates overnight at 4°C in 50 mM sodium carbonate buffer. .. After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C.

    Generated:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C. .. Fluorescent product generated over 30 minutes at 37°C was measured using a plate-reading spectrofluorimeter (Packard Fluorocount) set for 485 nm excitation and 590 nm emission.

    Recombinant:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: After any remaining protein binding sites were blocked with 1% normal mouse serum in Tris-buffered saline supplemented with 0.05% Tween-20 (TTBS, pH 7.4), wells were washed four times in TTBS, then human CSF at 1% or varying concentrations of recombinant human S100β standards (Genway #288258) prepared in normal mouse serum in TTBS supplemented with 1 mM CaCl2 were added for 90 minutes at 22°C (100 µl per well). .. After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C.

    Immunofluorescence:

    Article Title: Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage
    Article Snippet: Paragraph title: Sandwich enzyme-linked immunofluorescence assays ... After another four washes in TTBS, the streptavidin-horseradish peroxidase reporter probe was added (Invitrogen; 1/20,000 in 0.2% bovine serum albumin in TTBS/1 mM CaCl2 ) and incubated for 1 hour at 22°C.

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    Thermo Fisher hrp conjugated streptavidin
    Restoring protein synthesis delays retinal degeneration. (A) Protein synthesis levels depicted by the detection of azidohomoalanine incorporation into proteins with <t>HRP-conjugated</t> <t>streptavidin</t> and (B) coomassie staining for normalization of the membrane. (C) Graph showing relative levels of protein synthesis in the retinas of C57BL/6J (n = 3), rd16 (n = 3), and rd16 4ebp1/2 −/− (n = 3) mice at P15. (D) ERG waveforms of three groups and (E) graph showing mean ERG a- and b- wave amplitudes of C57BL/6J (n = 4), rd16 (n = 6), and rd16 4ebp1/2 −/− (n = 6) mice at P17. (F) Representative images of H E-stained retinal sections taken at P18 in the regions denoted as * in (G). (G) Spider plots depicting number of photoreceptor nuclei in the ONL as counted by a masked investigator. The spidergram was generated by plotting the number of nuclei using 200 μm step in the distance from the ONH for both hemispheres. (H) TUNEL staining of retinal sections from C57BL/6J (n = 3), rd16 (n = 4), and rd16 4ebp1/2 −/− (n = 3) eyes at P15. (I) Graph showing TUNEL-positive nuclei per square millimeter in the three groups. Green, TUNEL; blue, DAPI; a.u, arbitrary units; kD, kilodaltons. Error bars: SEM. Statistical significance denoted by *P
    Hrp Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated streptavidin/product/Thermo Fisher
    Average 90 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
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    97
    Thermo Fisher streptavidin hrp conjugates
    Correlation between apoptotic signaling and the generation of large DNAs with decreased generation of excised oligonucleotide repair products. ( A ) Analysis of large DNA fragments and excised oligomers released from genomic DNA following UV irradiation. HeLa cells were exposed to 20 J/m 2 of UVC and incubated for the indicated time points. After extraction of low molecular weight of DNAs from the cells, the DNA molecules were labeled with biotin at 3′-end using terminal deoxynucleotidyl transferase (TdT). The labeled DNA molecules were separated by gel electrophoresis, transferred to a nylon membrane and immobilized by UV cross-linking. The membrane was then incubated with <t>HRP-conjugated</t> <t>streptavidin,</t> and the labeled DNA molecules were detected with a chemiluminescence reagent (upper panel). A small portion of the UV-irradiated cells were lysed and analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. ( B ) Quantification of the excised oligonucleotide repair products and cleaved PARP signals as shown in ( A ). The relative signal intensities were determined by setting the highest signal to 100. The results were quantified and are plotted as mean and standard deviations. ( C ) Quantitative analysis of the excised repair products and the large DNA molecules. ( D ) Wild-type (AA8) and XPG mutant (UV135) CHO cell lines were exposed to UV and processed as described above for the detection of soluble DNAs.
    Streptavidin Hrp Conjugates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin hrp conjugates/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Thermo Fisher streptavidin poly hrp
    H4 10 Bpa cross-linking patterns of the mutant Mediator complexes. SDS-PAGE blot probed with <t>streptavidin</t> <t>poly-HRP</t> to detect and compare the pattern of biotinylated peptide cross-linked to Mediator subunits in WT Mediator and the mutant Mediator complexes. Equimolar amounts (∼7.5 nM) of each indicated Mediator complex were incubated with H4 10 Bpa (4 µM) and exposed to UV for 15 min. Asterisk refers to the same weak signal as in Fig. 2-A.
    Streptavidin Poly Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin poly hrp/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    streptavidin poly hrp - by Bioz Stars, 2020-04
    93/100 stars
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    Image Search Results


    Restoring protein synthesis delays retinal degeneration. (A) Protein synthesis levels depicted by the detection of azidohomoalanine incorporation into proteins with HRP-conjugated streptavidin and (B) coomassie staining for normalization of the membrane. (C) Graph showing relative levels of protein synthesis in the retinas of C57BL/6J (n = 3), rd16 (n = 3), and rd16 4ebp1/2 −/− (n = 3) mice at P15. (D) ERG waveforms of three groups and (E) graph showing mean ERG a- and b- wave amplitudes of C57BL/6J (n = 4), rd16 (n = 6), and rd16 4ebp1/2 −/− (n = 6) mice at P17. (F) Representative images of H E-stained retinal sections taken at P18 in the regions denoted as * in (G). (G) Spider plots depicting number of photoreceptor nuclei in the ONL as counted by a masked investigator. The spidergram was generated by plotting the number of nuclei using 200 μm step in the distance from the ONH for both hemispheres. (H) TUNEL staining of retinal sections from C57BL/6J (n = 3), rd16 (n = 4), and rd16 4ebp1/2 −/− (n = 3) eyes at P15. (I) Graph showing TUNEL-positive nuclei per square millimeter in the three groups. Green, TUNEL; blue, DAPI; a.u, arbitrary units; kD, kilodaltons. Error bars: SEM. Statistical significance denoted by *P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Role of Translational Attenuation in Inherited Retinal Degeneration

    doi: 10.1167/iovs.19-27512

    Figure Lengend Snippet: Restoring protein synthesis delays retinal degeneration. (A) Protein synthesis levels depicted by the detection of azidohomoalanine incorporation into proteins with HRP-conjugated streptavidin and (B) coomassie staining for normalization of the membrane. (C) Graph showing relative levels of protein synthesis in the retinas of C57BL/6J (n = 3), rd16 (n = 3), and rd16 4ebp1/2 −/− (n = 3) mice at P15. (D) ERG waveforms of three groups and (E) graph showing mean ERG a- and b- wave amplitudes of C57BL/6J (n = 4), rd16 (n = 6), and rd16 4ebp1/2 −/− (n = 6) mice at P17. (F) Representative images of H E-stained retinal sections taken at P18 in the regions denoted as * in (G). (G) Spider plots depicting number of photoreceptor nuclei in the ONL as counted by a masked investigator. The spidergram was generated by plotting the number of nuclei using 200 μm step in the distance from the ONH for both hemispheres. (H) TUNEL staining of retinal sections from C57BL/6J (n = 3), rd16 (n = 4), and rd16 4ebp1/2 −/− (n = 3) eyes at P15. (I) Graph showing TUNEL-positive nuclei per square millimeter in the three groups. Green, TUNEL; blue, DAPI; a.u, arbitrary units; kD, kilodaltons. Error bars: SEM. Statistical significance denoted by *P

    Article Snippet: Biotinylated proteins then were probed with HRP-conjugated streptavidin (S911; Thermo Fisher Scientific), incubated in ECL substrate (RPN2232; GE Healthcare, Chicago, IL, USA) and then imaged using a LI-COR (Lincoln, NE, USA) Fc imaging system.

    Techniques: Staining, Mouse Assay, Generated, TUNEL Assay

    Correlation between apoptotic signaling and the generation of large DNAs with decreased generation of excised oligonucleotide repair products. ( A ) Analysis of large DNA fragments and excised oligomers released from genomic DNA following UV irradiation. HeLa cells were exposed to 20 J/m 2 of UVC and incubated for the indicated time points. After extraction of low molecular weight of DNAs from the cells, the DNA molecules were labeled with biotin at 3′-end using terminal deoxynucleotidyl transferase (TdT). The labeled DNA molecules were separated by gel electrophoresis, transferred to a nylon membrane and immobilized by UV cross-linking. The membrane was then incubated with HRP-conjugated streptavidin, and the labeled DNA molecules were detected with a chemiluminescence reagent (upper panel). A small portion of the UV-irradiated cells were lysed and analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. ( B ) Quantification of the excised oligonucleotide repair products and cleaved PARP signals as shown in ( A ). The relative signal intensities were determined by setting the highest signal to 100. The results were quantified and are plotted as mean and standard deviations. ( C ) Quantitative analysis of the excised repair products and the large DNA molecules. ( D ) Wild-type (AA8) and XPG mutant (UV135) CHO cell lines were exposed to UV and processed as described above for the detection of soluble DNAs.

    Journal: Scientific Reports

    Article Title: Simultaneous detection of nucleotide excision repair events and apoptosis-induced DNA fragmentation in genotoxin-treated cells

    doi: 10.1038/s41598-018-20527-6

    Figure Lengend Snippet: Correlation between apoptotic signaling and the generation of large DNAs with decreased generation of excised oligonucleotide repair products. ( A ) Analysis of large DNA fragments and excised oligomers released from genomic DNA following UV irradiation. HeLa cells were exposed to 20 J/m 2 of UVC and incubated for the indicated time points. After extraction of low molecular weight of DNAs from the cells, the DNA molecules were labeled with biotin at 3′-end using terminal deoxynucleotidyl transferase (TdT). The labeled DNA molecules were separated by gel electrophoresis, transferred to a nylon membrane and immobilized by UV cross-linking. The membrane was then incubated with HRP-conjugated streptavidin, and the labeled DNA molecules were detected with a chemiluminescence reagent (upper panel). A small portion of the UV-irradiated cells were lysed and analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. ( B ) Quantification of the excised oligonucleotide repair products and cleaved PARP signals as shown in ( A ). The relative signal intensities were determined by setting the highest signal to 100. The results were quantified and are plotted as mean and standard deviations. ( C ) Quantitative analysis of the excised repair products and the large DNA molecules. ( D ) Wild-type (AA8) and XPG mutant (UV135) CHO cell lines were exposed to UV and processed as described above for the detection of soluble DNAs.

    Article Snippet: The entire blot was probed with streptavidin-HRP conjugates, and the entire image is displayed in each of the figures along with longer exposures of the region of the gel corresponding to the excised products of repair.

    Techniques: Irradiation, Incubation, Molecular Weight, Labeling, Nucleic Acid Electrophoresis, SDS Page, Mutagenesis

    H4 10 Bpa cross-linking patterns of the mutant Mediator complexes. SDS-PAGE blot probed with streptavidin poly-HRP to detect and compare the pattern of biotinylated peptide cross-linked to Mediator subunits in WT Mediator and the mutant Mediator complexes. Equimolar amounts (∼7.5 nM) of each indicated Mediator complex were incubated with H4 10 Bpa (4 µM) and exposed to UV for 15 min. Asterisk refers to the same weak signal as in Fig. 2-A.

    Journal: PLoS ONE

    Article Title: Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions

    doi: 10.1371/journal.pone.0038416

    Figure Lengend Snippet: H4 10 Bpa cross-linking patterns of the mutant Mediator complexes. SDS-PAGE blot probed with streptavidin poly-HRP to detect and compare the pattern of biotinylated peptide cross-linked to Mediator subunits in WT Mediator and the mutant Mediator complexes. Equimolar amounts (∼7.5 nM) of each indicated Mediator complex were incubated with H4 10 Bpa (4 µM) and exposed to UV for 15 min. Asterisk refers to the same weak signal as in Fig. 2-A.

    Article Snippet: SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits resolved on 10% SDS-polyacrylamide.

    Techniques: Mutagenesis, SDS Page, Incubation

    H4 10 Bpa has four specific cross-linking targets within Mediator. (A) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits. WT H4, H4 10 Bpa and H4 22 Bpa (4 µM) were incubated in the presence or absence of the WT Mediator complex (∼7.5 nM) and exposed to UV for 15 min when indicated. Cross-linking products were resolved on 6% SDS-polyacrylamide gel, transferred to to PVDF, detected by streptavidin poly-HRP, and referred to as BCTs (H4 10 B pa C ross-linking T argets). A weak band with relatively poor reproducibility was asterisked. (B) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits after different UV exposure times. Identical mixtures, which contain 7.5 nM Mediator complex and 4 µM H4 10 Bpa, were exposed to UV for 0, 5, 10 or 15 min. (C) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits in reactions with varient H4 10 Bpa concentration. WT Mediator complex (∼7.5 nM) was incubated with 1 µM, 2 µM or 4 µM H4 10 Bpa peptide and exposed to UV irradiation for 15 min. (D) Western blot analysis of histone tail peptide binding experiment comparing Mediator binding affinity for H4 10 Bpa and H2B tail peptide under the identical concentrations to the cross-linking reactions. WT Mediator complex (∼3 nM) was mixed with H4 10 Bpa (4 µM) or synthetic biotinylated histone H2B N’-tail peptide (4 µM or 8 µM) as the inputs. The basic steps and layout of the analysis were as described earlier ( Fig. 1 ). (E) and (F) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits, after H4 22 Bpa (E) or H2B tail peptide (F) was added at the indicated concentration.

    Journal: PLoS ONE

    Article Title: Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions

    doi: 10.1371/journal.pone.0038416

    Figure Lengend Snippet: H4 10 Bpa has four specific cross-linking targets within Mediator. (A) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits. WT H4, H4 10 Bpa and H4 22 Bpa (4 µM) were incubated in the presence or absence of the WT Mediator complex (∼7.5 nM) and exposed to UV for 15 min when indicated. Cross-linking products were resolved on 6% SDS-polyacrylamide gel, transferred to to PVDF, detected by streptavidin poly-HRP, and referred to as BCTs (H4 10 B pa C ross-linking T argets). A weak band with relatively poor reproducibility was asterisked. (B) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits after different UV exposure times. Identical mixtures, which contain 7.5 nM Mediator complex and 4 µM H4 10 Bpa, were exposed to UV for 0, 5, 10 or 15 min. (C) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits in reactions with varient H4 10 Bpa concentration. WT Mediator complex (∼7.5 nM) was incubated with 1 µM, 2 µM or 4 µM H4 10 Bpa peptide and exposed to UV irradiation for 15 min. (D) Western blot analysis of histone tail peptide binding experiment comparing Mediator binding affinity for H4 10 Bpa and H2B tail peptide under the identical concentrations to the cross-linking reactions. WT Mediator complex (∼3 nM) was mixed with H4 10 Bpa (4 µM) or synthetic biotinylated histone H2B N’-tail peptide (4 µM or 8 µM) as the inputs. The basic steps and layout of the analysis were as described earlier ( Fig. 1 ). (E) and (F) SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits, after H4 22 Bpa (E) or H2B tail peptide (F) was added at the indicated concentration.

    Article Snippet: SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits resolved on 10% SDS-polyacrylamide.

    Techniques: SDS Page, Incubation, Concentration Assay, Irradiation, Western Blot, Binding Assay

    Med5(Nut1)p, Med14(Rgr1)p, Med17(Srb4)p and Med1p are H4 10 Bpa cross-linking targets. Silver staining (A) and immunoblotting analysis (B) comparing the composition of affinity-purified MYC -tagged and non- MYC -tagged WT Mediator complexes after 10% SDS-PAGE. (C) A comparison of the cross-linking patterns of MYC -tagged Mediator complexes with the WT pattern using an SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits. Each indicated Mediator species (∼7.5 nM) was incubated with H4 10 Bpa (4 µM). A ‘Long Exposure’ of the 79 kD region on the SDS-PAGE blot probed with streptavidin poly-HRP is shown for a better view of the weak BCT4 signal in each sample.

    Journal: PLoS ONE

    Article Title: Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions

    doi: 10.1371/journal.pone.0038416

    Figure Lengend Snippet: Med5(Nut1)p, Med14(Rgr1)p, Med17(Srb4)p and Med1p are H4 10 Bpa cross-linking targets. Silver staining (A) and immunoblotting analysis (B) comparing the composition of affinity-purified MYC -tagged and non- MYC -tagged WT Mediator complexes after 10% SDS-PAGE. (C) A comparison of the cross-linking patterns of MYC -tagged Mediator complexes with the WT pattern using an SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated H4 10 Bpa peptide cross-linked to Mediator subunits. Each indicated Mediator species (∼7.5 nM) was incubated with H4 10 Bpa (4 µM). A ‘Long Exposure’ of the 79 kD region on the SDS-PAGE blot probed with streptavidin poly-HRP is shown for a better view of the weak BCT4 signal in each sample.

    Article Snippet: SDS-PAGE blot probed with streptavidin poly-HRP to detect biotinylated peptide cross-linked to Mediator subunits resolved on 10% SDS-polyacrylamide.

    Techniques: Silver Staining, Affinity Purification, SDS Page, Incubation